Experiment: R2A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS04440 and CA265_RS04445 are separated by 106 nucleotides CA265_RS04445 and CA265_RS04450 overlap by 10 nucleotides CA265_RS04450 and CA265_RS04455 are separated by 36 nucleotides
CA265_RS04440: CA265_RS04440 - orotidine-5'-phosphate decarboxylase, at 1,065,026 to 1,065,856
_RS04440
CA265_RS04445: CA265_RS04445 - hypothetical protein, at 1,065,963 to 1,066,217
_RS04445
CA265_RS04450: CA265_RS04450 - hypothetical protein, at 1,066,208 to 1,066,531
_RS04450
CA265_RS04455: CA265_RS04455 - hypothetical protein, at 1,066,568 to 1,067,851
_RS04455
Position (kb)
1065
1066
1067 Strain fitness (log2 ratio)
-2
-1
0
1 at 1065.023 kb on + strand at 1065.025 kb on + strand at 1065.849 kb on + strand at 1065.890 kb on - strand at 1065.890 kb on - strand at 1065.894 kb on - strand at 1065.939 kb on + strand at 1065.958 kb on - strand at 1065.962 kb on + strand at 1065.962 kb on + strand at 1065.963 kb on - strand at 1065.993 kb on - strand, within CA265_RS04445 at 1066.125 kb on - strand, within CA265_RS04445 at 1066.194 kb on - strand at 1066.215 kb on + strand at 1066.313 kb on + strand, within CA265_RS04450 at 1066.314 kb on - strand, within CA265_RS04450 at 1066.314 kb on - strand, within CA265_RS04450 at 1066.365 kb on - strand, within CA265_RS04450 at 1066.367 kb on + strand, within CA265_RS04450 at 1066.368 kb on - strand, within CA265_RS04450 at 1066.378 kb on + strand, within CA265_RS04450 at 1066.379 kb on - strand, within CA265_RS04450 at 1066.421 kb on - strand, within CA265_RS04450 at 1066.485 kb on - strand, within CA265_RS04450 at 1066.485 kb on - strand, within CA265_RS04450 at 1066.527 kb on + strand at 1066.540 kb on + strand at 1066.540 kb on + strand at 1066.595 kb on + strand at 1066.596 kb on - strand at 1066.753 kb on - strand, within CA265_RS04455 at 1066.766 kb on - strand, within CA265_RS04455 at 1066.766 kb on - strand, within CA265_RS04455 at 1066.821 kb on + strand, within CA265_RS04455 at 1066.833 kb on + strand, within CA265_RS04455 at 1066.833 kb on + strand, within CA265_RS04455 at 1066.834 kb on - strand, within CA265_RS04455 at 1066.834 kb on - strand, within CA265_RS04455 at 1066.881 kb on + strand, within CA265_RS04455 at 1066.932 kb on - strand, within CA265_RS04455 at 1066.980 kb on + strand, within CA265_RS04455 at 1066.980 kb on + strand, within CA265_RS04455 at 1066.985 kb on - strand, within CA265_RS04455 at 1067.028 kb on + strand, within CA265_RS04455
Per-strain Table
Position Strand Gene LocusTag Fraction R2A remove 1,065,023 + +0.7 1,065,025 + +0.0 1,065,849 + +0.1 1,065,890 - +0.4 1,065,890 - -0.2 1,065,894 - +0.2 1,065,939 + +0.2 1,065,958 - -0.5 1,065,962 + -0.4 1,065,962 + +0.1 1,065,963 - -0.0 1,065,993 - CA265_RS04445 0.12 +0.6 1,066,125 - CA265_RS04445 0.64 -0.1 1,066,194 - -0.2 1,066,215 + -0.6 1,066,313 + CA265_RS04450 0.32 -0.0 1,066,314 - CA265_RS04450 0.33 +0.4 1,066,314 - CA265_RS04450 0.33 -0.8 1,066,365 - CA265_RS04450 0.48 +0.5 1,066,367 + CA265_RS04450 0.49 -0.1 1,066,368 - CA265_RS04450 0.49 -0.7 1,066,378 + CA265_RS04450 0.52 -0.3 1,066,379 - CA265_RS04450 0.53 -0.5 1,066,421 - CA265_RS04450 0.66 +0.5 1,066,485 - CA265_RS04450 0.85 -0.8 1,066,485 - CA265_RS04450 0.85 -0.7 1,066,527 + +0.3 1,066,540 + +1.0 1,066,540 + +0.3 1,066,595 + -0.2 1,066,596 - -2.0 1,066,753 - CA265_RS04455 0.14 -1.2 1,066,766 - CA265_RS04455 0.15 +0.2 1,066,766 - CA265_RS04455 0.15 +0.2 1,066,821 + CA265_RS04455 0.20 +0.3 1,066,833 + CA265_RS04455 0.21 -2.7 1,066,833 + CA265_RS04455 0.21 -1.3 1,066,834 - CA265_RS04455 0.21 -1.2 1,066,834 - CA265_RS04455 0.21 +0.8 1,066,881 + CA265_RS04455 0.24 -0.4 1,066,932 - CA265_RS04455 0.28 -2.0 1,066,980 + CA265_RS04455 0.32 -0.2 1,066,980 + CA265_RS04455 0.32 -0.4 1,066,985 - CA265_RS04455 0.32 -0.3 1,067,028 + CA265_RS04455 0.36 -1.6
Or see this region's nucleotide sequence