Experiment: R2A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS01945 and CA265_RS01950 are separated by 178 nucleotides CA265_RS01950 and CA265_RS01955 overlap by 14 nucleotides CA265_RS01955 and CA265_RS01960 are separated by 459 nucleotides
CA265_RS01945: CA265_RS01945 - hypothetical protein, at 493,846 to 496,143
_RS01945
CA265_RS01950: CA265_RS01950 - hypothetical protein, at 496,322 to 496,552
_RS01950
CA265_RS01955: CA265_RS01955 - VapC toxin family PIN domain ribonuclease, at 496,539 to 496,930
_RS01955
CA265_RS01960: CA265_RS01960 - division/cell wall cluster transcriptional repressor MraZ, at 497,390 to 497,857
_RS01960
Position (kb)
496
497 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 495.331 kb on + strand, within CA265_RS01945 at 495.359 kb on - strand, within CA265_RS01945 at 495.433 kb on + strand, within CA265_RS01945 at 495.444 kb on + strand, within CA265_RS01945 at 495.568 kb on + strand, within CA265_RS01945 at 495.591 kb on + strand, within CA265_RS01945 at 495.592 kb on - strand, within CA265_RS01945 at 495.629 kb on - strand, within CA265_RS01945 at 495.630 kb on + strand, within CA265_RS01945 at 495.631 kb on - strand, within CA265_RS01945 at 495.649 kb on - strand, within CA265_RS01945 at 495.649 kb on - strand, within CA265_RS01945 at 495.710 kb on + strand, within CA265_RS01945 at 495.778 kb on + strand, within CA265_RS01945 at 495.778 kb on + strand, within CA265_RS01945 at 495.779 kb on - strand, within CA265_RS01945 at 495.779 kb on - strand, within CA265_RS01945 at 495.779 kb on - strand, within CA265_RS01945 at 495.855 kb on + strand, within CA265_RS01945 at 495.858 kb on - strand, within CA265_RS01945 at 495.889 kb on + strand, within CA265_RS01945 at 495.890 kb on - strand, within CA265_RS01945 at 495.892 kb on - strand, within CA265_RS01945 at 495.892 kb on - strand, within CA265_RS01945 at 496.032 kb on + strand at 496.062 kb on + strand at 496.063 kb on - strand at 496.075 kb on + strand at 496.141 kb on + strand at 496.142 kb on - strand at 496.145 kb on + strand at 496.145 kb on + strand at 496.176 kb on + strand at 496.196 kb on - strand at 496.244 kb on + strand at 496.245 kb on - strand at 496.274 kb on - strand at 496.284 kb on + strand at 496.285 kb on - strand at 496.306 kb on - strand at 496.310 kb on - strand at 496.329 kb on + strand at 496.339 kb on + strand at 496.385 kb on - strand, within CA265_RS01950 at 496.389 kb on + strand, within CA265_RS01950 at 496.408 kb on - strand, within CA265_RS01950 at 496.477 kb on + strand, within CA265_RS01950 at 496.504 kb on + strand, within CA265_RS01950 at 496.504 kb on + strand, within CA265_RS01950 at 496.504 kb on + strand, within CA265_RS01950 at 496.550 kb on + strand at 496.558 kb on + strand at 496.558 kb on + strand at 496.559 kb on - strand at 496.565 kb on + strand at 496.565 kb on + strand at 496.568 kb on + strand at 496.568 kb on + strand at 496.569 kb on - strand at 496.569 kb on - strand at 496.569 kb on - strand at 496.569 kb on - strand at 496.583 kb on - strand, within CA265_RS01955 at 496.668 kb on - strand, within CA265_RS01955 at 496.668 kb on - strand, within CA265_RS01955 at 496.738 kb on + strand, within CA265_RS01955 at 496.831 kb on + strand, within CA265_RS01955 at 496.848 kb on + strand, within CA265_RS01955 at 496.849 kb on - strand, within CA265_RS01955 at 496.913 kb on - strand at 497.138 kb on - strand at 497.156 kb on - strand at 497.167 kb on - strand at 497.220 kb on + strand at 497.221 kb on - strand at 497.263 kb on + strand at 497.263 kb on + strand at 497.274 kb on + strand at 497.289 kb on - strand at 497.500 kb on + strand, within CA265_RS01960
Per-strain Table
Position Strand Gene LocusTag Fraction R2A remove 495,331 + CA265_RS01945 0.65 -0.6 495,359 - CA265_RS01945 0.66 +0.3 495,433 + CA265_RS01945 0.69 +0.2 495,444 + CA265_RS01945 0.70 -0.6 495,568 + CA265_RS01945 0.75 -0.8 495,591 + CA265_RS01945 0.76 -0.9 495,592 - CA265_RS01945 0.76 +0.8 495,629 - CA265_RS01945 0.78 -1.2 495,630 + CA265_RS01945 0.78 -0.3 495,631 - CA265_RS01945 0.78 -0.3 495,649 - CA265_RS01945 0.78 -0.5 495,649 - CA265_RS01945 0.78 -0.7 495,710 + CA265_RS01945 0.81 -0.4 495,778 + CA265_RS01945 0.84 -0.6 495,778 + CA265_RS01945 0.84 -0.1 495,779 - CA265_RS01945 0.84 +0.4 495,779 - CA265_RS01945 0.84 -0.6 495,779 - CA265_RS01945 0.84 +0.1 495,855 + CA265_RS01945 0.87 -0.7 495,858 - CA265_RS01945 0.88 +0.2 495,889 + CA265_RS01945 0.89 +0.8 495,890 - CA265_RS01945 0.89 -1.0 495,892 - CA265_RS01945 0.89 +0.4 495,892 - CA265_RS01945 0.89 -0.4 496,032 + -0.4 496,062 + -0.9 496,063 - -0.7 496,075 + +1.3 496,141 + +0.5 496,142 - +0.4 496,145 + +0.9 496,145 + +0.2 496,176 + +0.2 496,196 - +0.8 496,244 + -0.1 496,245 - +0.7 496,274 - -0.2 496,284 + -0.3 496,285 - +0.0 496,306 - +0.9 496,310 - +1.6 496,329 + -0.2 496,339 + +0.2 496,385 - CA265_RS01950 0.27 -0.7 496,389 + CA265_RS01950 0.29 +0.5 496,408 - CA265_RS01950 0.37 -0.9 496,477 + CA265_RS01950 0.67 +0.5 496,504 + CA265_RS01950 0.79 +0.6 496,504 + CA265_RS01950 0.79 -0.0 496,504 + CA265_RS01950 0.79 +1.2 496,550 + +0.3 496,558 + -1.4 496,558 + +0.2 496,559 - -0.5 496,565 + -0.8 496,565 + +0.6 496,568 + +0.4 496,568 + +0.0 496,569 - -0.1 496,569 - -0.1 496,569 - +0.7 496,569 - +0.0 496,583 - CA265_RS01955 0.11 +0.4 496,668 - CA265_RS01955 0.33 -0.3 496,668 - CA265_RS01955 0.33 +0.3 496,738 + CA265_RS01955 0.51 -0.2 496,831 + CA265_RS01955 0.74 +0.3 496,848 + CA265_RS01955 0.79 -0.7 496,849 - CA265_RS01955 0.79 +0.1 496,913 - +0.6 497,138 - -0.1 497,156 - +0.6 497,167 - -0.5 497,220 + +0.2 497,221 - -0.1 497,263 + -0.4 497,263 + +2.3 497,274 + -1.9 497,289 - +0.5 497,500 + CA265_RS01960 0.24 -0.6
Or see this region's nucleotide sequence