Experiment: R2A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS01215 and CA265_RS01220 overlap by 8 nucleotides CA265_RS01220 and CA265_RS01225 are separated by 2 nucleotides CA265_RS01225 and CA265_RS01230 are separated by 404 nucleotides
CA265_RS01215: CA265_RS01215 - sterol desaturase, at 302,503 to 303,465
_RS01215
CA265_RS01220: CA265_RS01220 - hypothetical protein, at 303,458 to 303,658
_RS01220
CA265_RS01225: CA265_RS01225 - hypothetical protein, at 303,661 to 303,921
_RS01225
CA265_RS01230: CA265_RS01230 - hypothetical protein, at 304,326 to 306,656
_RS01230
Position (kb)
303
304 Strain fitness (log2 ratio)
-2
-1
0
1 at 302.665 kb on - strand, within CA265_RS01215 at 302.718 kb on - strand, within CA265_RS01215 at 302.725 kb on - strand, within CA265_RS01215 at 302.801 kb on + strand, within CA265_RS01215 at 302.823 kb on + strand, within CA265_RS01215 at 302.826 kb on + strand, within CA265_RS01215 at 302.906 kb on + strand, within CA265_RS01215 at 302.911 kb on - strand, within CA265_RS01215 at 302.931 kb on + strand, within CA265_RS01215 at 302.934 kb on + strand, within CA265_RS01215 at 302.935 kb on - strand, within CA265_RS01215 at 302.955 kb on + strand, within CA265_RS01215 at 302.961 kb on + strand, within CA265_RS01215 at 303.004 kb on + strand, within CA265_RS01215 at 303.030 kb on - strand, within CA265_RS01215 at 303.053 kb on + strand, within CA265_RS01215 at 303.053 kb on + strand, within CA265_RS01215 at 303.053 kb on + strand, within CA265_RS01215 at 303.054 kb on - strand, within CA265_RS01215 at 303.092 kb on + strand, within CA265_RS01215 at 303.093 kb on - strand, within CA265_RS01215 at 303.154 kb on - strand, within CA265_RS01215 at 303.184 kb on + strand, within CA265_RS01215 at 303.250 kb on + strand, within CA265_RS01215 at 303.250 kb on + strand, within CA265_RS01215 at 303.268 kb on + strand, within CA265_RS01215 at 303.275 kb on + strand, within CA265_RS01215 at 303.327 kb on + strand, within CA265_RS01215 at 303.327 kb on + strand, within CA265_RS01215 at 303.328 kb on - strand, within CA265_RS01215 at 303.366 kb on - strand, within CA265_RS01215 at 303.367 kb on + strand, within CA265_RS01215 at 303.497 kb on + strand, within CA265_RS01220 at 303.595 kb on - strand, within CA265_RS01220 at 303.595 kb on - strand, within CA265_RS01220 at 303.623 kb on + strand, within CA265_RS01220 at 303.640 kb on + strand at 303.681 kb on - strand at 303.711 kb on + strand, within CA265_RS01225 at 303.730 kb on - strand, within CA265_RS01225 at 303.759 kb on + strand, within CA265_RS01225 at 303.759 kb on + strand, within CA265_RS01225 at 303.814 kb on - strand, within CA265_RS01225 at 303.834 kb on + strand, within CA265_RS01225 at 303.891 kb on + strand, within CA265_RS01225 at 303.967 kb on - strand at 303.990 kb on - strand at 304.002 kb on + strand at 304.131 kb on + strand at 304.131 kb on + strand at 304.132 kb on - strand at 304.198 kb on - strand at 304.272 kb on + strand at 304.273 kb on - strand at 304.273 kb on - strand at 304.367 kb on - strand at 304.453 kb on + strand at 304.490 kb on - strand at 304.502 kb on + strand at 304.530 kb on - strand at 304.550 kb on - strand at 304.555 kb on + strand at 304.590 kb on - strand, within CA265_RS01230 at 304.592 kb on - strand, within CA265_RS01230 at 304.739 kb on + strand, within CA265_RS01230 at 304.747 kb on - strand, within CA265_RS01230 at 304.749 kb on - strand, within CA265_RS01230 at 304.749 kb on - strand, within CA265_RS01230 at 304.773 kb on + strand, within CA265_RS01230 at 304.774 kb on - strand, within CA265_RS01230 at 304.776 kb on + strand, within CA265_RS01230 at 304.776 kb on + strand, within CA265_RS01230 at 304.777 kb on - strand, within CA265_RS01230 at 304.919 kb on + strand, within CA265_RS01230 at 304.919 kb on + strand, within CA265_RS01230 at 304.919 kb on + strand, within CA265_RS01230
Per-strain Table
Position Strand Gene LocusTag Fraction R2A remove 302,665 - CA265_RS01215 0.17 +0.1 302,718 - CA265_RS01215 0.22 -1.1 302,725 - CA265_RS01215 0.23 -0.9 302,801 + CA265_RS01215 0.31 -0.1 302,823 + CA265_RS01215 0.33 +0.7 302,826 + CA265_RS01215 0.34 +0.2 302,906 + CA265_RS01215 0.42 -0.4 302,911 - CA265_RS01215 0.42 -0.2 302,931 + CA265_RS01215 0.44 +0.6 302,934 + CA265_RS01215 0.45 -0.3 302,935 - CA265_RS01215 0.45 -1.0 302,955 + CA265_RS01215 0.47 -0.3 302,961 + CA265_RS01215 0.48 +0.2 303,004 + CA265_RS01215 0.52 +0.1 303,030 - CA265_RS01215 0.55 -0.2 303,053 + CA265_RS01215 0.57 -2.6 303,053 + CA265_RS01215 0.57 -0.1 303,053 + CA265_RS01215 0.57 +0.4 303,054 - CA265_RS01215 0.57 +0.3 303,092 + CA265_RS01215 0.61 -1.3 303,093 - CA265_RS01215 0.61 +0.4 303,154 - CA265_RS01215 0.68 -0.0 303,184 + CA265_RS01215 0.71 +0.2 303,250 + CA265_RS01215 0.78 +0.5 303,250 + CA265_RS01215 0.78 -0.8 303,268 + CA265_RS01215 0.79 -0.5 303,275 + CA265_RS01215 0.80 +0.1 303,327 + CA265_RS01215 0.86 -0.7 303,327 + CA265_RS01215 0.86 -0.2 303,328 - CA265_RS01215 0.86 +0.8 303,366 - CA265_RS01215 0.90 -0.4 303,367 + CA265_RS01215 0.90 +0.1 303,497 + CA265_RS01220 0.19 +0.7 303,595 - CA265_RS01220 0.68 -0.2 303,595 - CA265_RS01220 0.68 -0.4 303,623 + CA265_RS01220 0.82 -0.5 303,640 + -0.5 303,681 - -0.4 303,711 + CA265_RS01225 0.19 +0.4 303,730 - CA265_RS01225 0.26 +0.8 303,759 + CA265_RS01225 0.38 +0.9 303,759 + CA265_RS01225 0.38 +0.2 303,814 - CA265_RS01225 0.59 -0.2 303,834 + CA265_RS01225 0.66 +0.5 303,891 + CA265_RS01225 0.88 -0.1 303,967 - +0.1 303,990 - -0.2 304,002 + -0.5 304,131 + -0.2 304,131 + -0.2 304,132 - -0.9 304,198 - -0.2 304,272 + +1.3 304,273 - -0.9 304,273 - +0.2 304,367 - +0.7 304,453 + -0.8 304,490 - +0.5 304,502 + -2.0 304,530 - -0.3 304,550 - -1.2 304,555 + -0.6 304,590 - CA265_RS01230 0.11 -0.9 304,592 - CA265_RS01230 0.11 -0.1 304,739 + CA265_RS01230 0.18 -0.3 304,747 - CA265_RS01230 0.18 +0.4 304,749 - CA265_RS01230 0.18 -0.4 304,749 - CA265_RS01230 0.18 -0.8 304,773 + CA265_RS01230 0.19 -0.3 304,774 - CA265_RS01230 0.19 +0.4 304,776 + CA265_RS01230 0.19 -0.3 304,776 + CA265_RS01230 0.19 +0.6 304,777 - CA265_RS01230 0.19 -0.1 304,919 + CA265_RS01230 0.25 -0.8 304,919 + CA265_RS01230 0.25 -1.5 304,919 + CA265_RS01230 0.25 +0.3
Or see this region's nucleotide sequence