Experiment: D-Gluconic Acid (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_2028 and Ac3H11_2029 are separated by 107 nucleotides Ac3H11_2029 and Ac3H11_2030 are separated by 18 nucleotides
Ac3H11_2028 - Methyl-accepting chemotaxis protein I (serine chemoreceptor protein), at 247,829 to 249,481
_2028
Ac3H11_2029 - General secretion pathway protein E / Type II secretion cytoplasmic ATP binding protein (PulE, ATPase), at 249,589 to 250,977
_2029
Ac3H11_2030 - General secretion pathway protein D / Type II secretion outermembrane pore forming protein (PulD), at 250,996 to 253,242
_2030
Position (kb) 249
250
251 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 249.310 kb on + strand, within Ac3H11_2028 at 249.311 kb on - strand, within Ac3H11_2028 at 249.401 kb on + strand at 249.401 kb on + strand at 249.401 kb on + strand at 249.401 kb on + strand at 249.401 kb on + strand at 249.502 kb on + strand at 249.503 kb on - strand at 249.524 kb on + strand at 249.524 kb on + strand at 249.525 kb on - strand at 249.630 kb on + strand at 249.630 kb on + strand at 249.839 kb on + strand, within Ac3H11_2029 at 249.840 kb on - strand, within Ac3H11_2029 at 249.840 kb on - strand, within Ac3H11_2029 at 250.137 kb on - strand, within Ac3H11_2029 at 250.202 kb on + strand, within Ac3H11_2029 at 250.202 kb on + strand, within Ac3H11_2029 at 250.202 kb on + strand, within Ac3H11_2029 at 250.202 kb on + strand, within Ac3H11_2029 at 250.203 kb on - strand, within Ac3H11_2029 at 250.203 kb on - strand, within Ac3H11_2029 at 250.784 kb on + strand, within Ac3H11_2029 at 250.998 kb on - strand at 250.998 kb on - strand at 250.998 kb on - strand at 250.998 kb on - strand at 251.306 kb on + strand, within Ac3H11_2030 at 251.306 kb on + strand, within Ac3H11_2030 at 251.306 kb on + strand, within Ac3H11_2030 at 251.307 kb on - strand, within Ac3H11_2030 at 251.444 kb on + strand, within Ac3H11_2030 at 251.444 kb on + strand, within Ac3H11_2030 at 251.445 kb on - strand, within Ac3H11_2030 at 251.445 kb on - strand, within Ac3H11_2030 at 251.445 kb on - strand, within Ac3H11_2030 at 251.567 kb on + strand, within Ac3H11_2030 at 251.568 kb on - strand, within Ac3H11_2030 at 251.702 kb on + strand, within Ac3H11_2030 at 251.702 kb on + strand, within Ac3H11_2030 at 251.702 kb on + strand, within Ac3H11_2030 at 251.702 kb on + strand, within Ac3H11_2030 at 251.703 kb on - strand, within Ac3H11_2030 at 251.703 kb on - strand, within Ac3H11_2030 at 251.703 kb on - strand, within Ac3H11_2030 at 251.703 kb on - strand, within Ac3H11_2030 at 251.703 kb on - strand, within Ac3H11_2030 at 251.705 kb on + strand, within Ac3H11_2030 at 251.959 kb on + strand, within Ac3H11_2030
Per-strain Table
Position Strand Gene LocusTag Fraction D-Gluconic Acid (C) remove 249,310 + Ac3H11_2028 0.90 -1.6 249,311 - Ac3H11_2028 0.90 +2.5 249,401 + -0.2 249,401 + -0.6 249,401 + -1.3 249,401 + +0.5 249,401 + -1.4 249,502 + +1.1 249,503 - -0.3 249,524 + -0.1 249,524 + +0.6 249,525 - +0.1 249,630 + -3.0 249,630 + -1.1 249,839 + Ac3H11_2029 0.18 -0.6 249,840 - Ac3H11_2029 0.18 -2.2 249,840 - Ac3H11_2029 0.18 -2.3 250,137 - Ac3H11_2029 0.39 +0.1 250,202 + Ac3H11_2029 0.44 -0.6 250,202 + Ac3H11_2029 0.44 -2.9 250,202 + Ac3H11_2029 0.44 -1.1 250,202 + Ac3H11_2029 0.44 -1.8 250,203 - Ac3H11_2029 0.44 -2.7 250,203 - Ac3H11_2029 0.44 +0.2 250,784 + Ac3H11_2029 0.86 -2.3 250,998 - +0.8 250,998 - -1.8 250,998 - +0.5 250,998 - +0.1 251,306 + Ac3H11_2030 0.14 -0.4 251,306 + Ac3H11_2030 0.14 +1.1 251,306 + Ac3H11_2030 0.14 -0.4 251,307 - Ac3H11_2030 0.14 -1.4 251,444 + Ac3H11_2030 0.20 -0.1 251,444 + Ac3H11_2030 0.20 -1.4 251,445 - Ac3H11_2030 0.20 -1.9 251,445 - Ac3H11_2030 0.20 +1.1 251,445 - Ac3H11_2030 0.20 +0.7 251,567 + Ac3H11_2030 0.25 -0.6 251,568 - Ac3H11_2030 0.25 +2.5 251,702 + Ac3H11_2030 0.31 -1.3 251,702 + Ac3H11_2030 0.31 +2.0 251,702 + Ac3H11_2030 0.31 -2.0 251,702 + Ac3H11_2030 0.31 -0.7 251,703 - Ac3H11_2030 0.31 -1.2 251,703 - Ac3H11_2030 0.31 -1.1 251,703 - Ac3H11_2030 0.31 -0.1 251,703 - Ac3H11_2030 0.31 -0.2 251,703 - Ac3H11_2030 0.31 -1.3 251,705 + Ac3H11_2030 0.32 -0.6 251,959 + Ac3H11_2030 0.43 -1.3
Or see this region's nucleotide sequence