Experiment: Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; 1,3-Propanediol_timecourse1; 20 generations
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS08775 and MMP_RS08780 are separated by 9 nucleotides MMP_RS08780 and MMP_RS08785 are separated by 5 nucleotides MMP_RS08785 and MMP_RS08790 are separated by 8 nucleotides MMP_RS08790 and MMP_RS08795 are separated by 113 nucleotides MMP_RS08795 and MMP_RS08800 are separated by 22 nucleotides MMP_RS08800 and MMP_RS08805 overlap by 44 nucleotides
MMP_RS08775: MMP_RS08775 - methanogenesis marker 9 domain-containing protein, at 1,645,101 to 1,645,556
_RS08775
MMP_RS08780: MMP_RS08780 - 2-amino-5-formylamino-6-ribosylaminopyrimidin-4(3H)-one 5'-monophosphate deformylase, at 1,645,566 to 1,646,231
_RS08780
MMP_RS08785: MMP_RS08785 - RNA-protein complex protein Nop10, at 1,646,237 to 1,646,392
_RS08785
MMP_RS08790: MMP_RS08790 - translation initiation factor IF-2 subunit alpha, at 1,646,401 to 1,647,198
_RS08790
MMP_RS08795: MMP_RS08795 - 30S ribosomal protein S27e, at 1,647,312 to 1,647,497
_RS08795
MMP_RS08800: MMP_RS08800 - 50S ribosomal protein L44e, at 1,647,520 to 1,647,801
_RS08800
MMP_RS08805: MMP_RS08805 - hypothetical protein, at 1,647,758 to 1,648,345
_RS08805
Position (kb)
1646
1647
1648 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1 at 1645.416 kb on - strand, within MMP_RS08775 at 1645.513 kb on + strand at 1645.556 kb on - strand at 1645.556 kb on - strand at 1645.559 kb on + strand at 1645.567 kb on - strand at 1645.740 kb on - strand at 1645.740 kb on - strand at 1645.804 kb on + strand at 1645.804 kb on + strand at 1645.838 kb on - strand at 1645.850 kb on - strand at 1645.851 kb on + strand at 1645.917 kb on - strand at 1645.919 kb on - strand at 1645.967 kb on + strand at 1645.980 kb on + strand at 1646.021 kb on + strand at 1646.072 kb on - strand at 1646.095 kb on - strand at 1646.175 kb on - strand at 1646.175 kb on - strand at 1646.231 kb on - strand at 1646.277 kb on - strand, within MMP_RS08785 at 1646.524 kb on - strand, within MMP_RS08790 at 1646.550 kb on + strand, within MMP_RS08790 at 1646.713 kb on - strand, within MMP_RS08790 at 1647.112 kb on - strand, within MMP_RS08790 at 1647.218 kb on - strand at 1647.219 kb on + strand at 1647.223 kb on - strand at 1647.224 kb on + strand at 1647.230 kb on + strand at 1647.240 kb on - strand at 1647.243 kb on + strand at 1647.250 kb on + strand at 1647.277 kb on - strand at 1647.278 kb on + strand at 1647.283 kb on - strand at 1647.304 kb on + strand at 1647.313 kb on - strand at 1647.319 kb on + strand at 1647.322 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; 1,3-Propanediol_timecourse1; 20 generations remove 1,645,416 - MMP_RS08775 0.69 +1.0 1,645,513 + +0.4 1,645,556 - +1.1 1,645,556 - +0.5 1,645,559 + +0.4 1,645,567 - +0.9 1,645,740 - -4.2 1,645,740 - -5.8 1,645,804 + -4.9 1,645,804 + -5.4 1,645,838 - -5.2 1,645,850 - -3.3 1,645,851 + -5.0 1,645,917 - -3.0 1,645,919 - -4.2 1,645,967 + -4.6 1,645,980 + -5.4 1,646,021 + -3.9 1,646,072 - -4.9 1,646,095 - -5.2 1,646,175 - -3.0 1,646,175 - -4.8 1,646,231 - -4.7 1,646,277 - MMP_RS08785 0.26 -2.5 1,646,524 - MMP_RS08790 0.15 -1.8 1,646,550 + MMP_RS08790 0.19 -5.6 1,646,713 - MMP_RS08790 0.39 -4.7 1,647,112 - MMP_RS08790 0.89 -3.0 1,647,218 - -5.7 1,647,219 + -4.5 1,647,223 - -4.5 1,647,224 + -4.7 1,647,230 + -3.0 1,647,240 - -4.8 1,647,243 + -5.3 1,647,250 + -1.8 1,647,277 - +0.6 1,647,278 + +1.0 1,647,283 - -0.2 1,647,304 + +0.1 1,647,313 - -1.9 1,647,319 + +0.9 1,647,322 + -0.5
Or see this region's nucleotide sequence