Experiment: Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; 1,3-Propanediol_timecourse1; 20 generations
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS08150 and MMP_RS08155 are separated by 102 nucleotides MMP_RS08155 and MMP_RS08160 are separated by 21 nucleotides MMP_RS08160 and MMP_RS08165 are separated by 32 nucleotides MMP_RS08165 and MMP_RS08170 are separated by 143 nucleotides
MMP_RS08150: MMP_RS08150 - agmatinase, at 1,534,843 to 1,535,691
_RS08150
MMP_RS08155: MMP_RS08155 - hypothetical protein, at 1,535,794 to 1,536,036
_RS08155
MMP_RS08160: MMP_RS08160 - EamA family transporter, at 1,536,058 to 1,536,471
_RS08160
MMP_RS08165: MMP_RS08165 - phosphoglycerate dehydrogenase, at 1,536,504 to 1,538,075
_RS08165
MMP_RS08170: MMP_RS08170 - glutamine-hydrolyzing carbamoyl-phosphate synthase small subunit, at 1,538,219 to 1,539,319
_RS08170
Position (kb) 1536
1537
1538
1539 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 1535.542 kb on + strand at 1535.544 kb on + strand at 1535.634 kb on - strand at 1535.677 kb on + strand at 1535.693 kb on + strand at 1535.750 kb on - strand at 1535.762 kb on - strand at 1535.763 kb on + strand at 1535.763 kb on + strand at 1535.810 kb on - strand at 1535.832 kb on + strand, within MMP_RS08155 at 1535.856 kb on - strand, within MMP_RS08155 at 1535.856 kb on - strand, within MMP_RS08155 at 1535.860 kb on - strand, within MMP_RS08155 at 1535.860 kb on - strand, within MMP_RS08155 at 1535.860 kb on - strand, within MMP_RS08155 at 1535.860 kb on - strand, within MMP_RS08155 at 1535.861 kb on + strand, within MMP_RS08155 at 1535.861 kb on + strand, within MMP_RS08155 at 1535.861 kb on + strand, within MMP_RS08155 at 1535.861 kb on + strand, within MMP_RS08155 at 1535.865 kb on + strand, within MMP_RS08155 at 1535.901 kb on + strand, within MMP_RS08155 at 1536.197 kb on + strand, within MMP_RS08160 at 1536.332 kb on + strand, within MMP_RS08160 at 1536.335 kb on + strand, within MMP_RS08160 at 1536.343 kb on - strand, within MMP_RS08160 at 1536.364 kb on - strand, within MMP_RS08160 at 1536.421 kb on + strand, within MMP_RS08160 at 1536.425 kb on + strand, within MMP_RS08160 at 1536.426 kb on - strand, within MMP_RS08160 at 1536.432 kb on + strand at 1536.506 kb on + strand at 1536.506 kb on + strand at 1536.532 kb on + strand at 1536.591 kb on - strand at 1536.651 kb on - strand at 1537.063 kb on + strand, within MMP_RS08165 at 1537.357 kb on - strand, within MMP_RS08165 at 1537.378 kb on + strand, within MMP_RS08165 at 1538.061 kb on + strand at 1538.077 kb on - strand at 1538.154 kb on + strand at 1538.154 kb on + strand at 1538.162 kb on - strand at 1538.709 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; 1,3-Propanediol_timecourse1; 20 generations remove 1,535,542 + -5.4 1,535,544 + -4.5 1,535,634 - -4.6 1,535,677 + -4.4 1,535,693 + +1.4 1,535,750 - -2.1 1,535,762 - -1.5 1,535,763 + -2.1 1,535,763 + -1.4 1,535,810 - -2.3 1,535,832 + MMP_RS08155 0.16 -1.5 1,535,856 - MMP_RS08155 0.26 -1.5 1,535,856 - MMP_RS08155 0.26 -2.2 1,535,860 - MMP_RS08155 0.27 -2.4 1,535,860 - MMP_RS08155 0.27 -1.9 1,535,860 - MMP_RS08155 0.27 -3.2 1,535,860 - MMP_RS08155 0.27 -1.6 1,535,861 + MMP_RS08155 0.28 -2.1 1,535,861 + MMP_RS08155 0.28 -2.2 1,535,861 + MMP_RS08155 0.28 -1.9 1,535,861 + MMP_RS08155 0.28 -2.5 1,535,865 + MMP_RS08155 0.29 -1.4 1,535,901 + MMP_RS08155 0.44 -1.6 1,536,197 + MMP_RS08160 0.34 +0.7 1,536,332 + MMP_RS08160 0.66 +1.2 1,536,335 + MMP_RS08160 0.67 +0.3 1,536,343 - MMP_RS08160 0.69 +0.8 1,536,364 - MMP_RS08160 0.74 +0.1 1,536,421 + MMP_RS08160 0.88 +0.3 1,536,425 + MMP_RS08160 0.89 +0.6 1,536,426 - MMP_RS08160 0.89 +1.4 1,536,432 + +1.0 1,536,506 + +1.1 1,536,506 + +0.1 1,536,532 + -0.3 1,536,591 - -2.4 1,536,651 - -1.3 1,537,063 + MMP_RS08165 0.36 -3.3 1,537,357 - MMP_RS08165 0.54 -2.5 1,537,378 + MMP_RS08165 0.56 -4.3 1,538,061 + -3.1 1,538,077 - -3.0 1,538,154 + +0.1 1,538,154 + +1.0 1,538,162 - +0.6 1,538,709 + -1.1
Or see this region's nucleotide sequence