Experiment: Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; 1,3-Propanediol_timecourse1; 20 generations
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS07530 and MMP_RS07535 are separated by 20 nucleotides MMP_RS07535 and MMP_RS07540 are separated by 15 nucleotides MMP_RS07540 and MMP_RS07545 are separated by 19 nucleotides MMP_RS07545 and MMP_RS07550 are separated by 13 nucleotides
MMP_RS07530: MMP_RS07530 - 4Fe-4S binding protein, at 1,426,400 to 1,426,885
_RS07530
MMP_RS07535: MMP_RS07535 - 4Fe-4S binding protein, at 1,426,906 to 1,427,664
_RS07535
MMP_RS07540: MMP_RS07540 - CBS domain-containing protein, at 1,427,680 to 1,428,321
_RS07540
MMP_RS07545: MMP_RS07545 - HTH domain-containing protein, at 1,428,341 to 1,428,736
_RS07545
MMP_RS07550: MMP_RS07550 - L-serine ammonia-lyase iron-sulfur-dependent subunit alpha, at 1,428,750 to 1,429,940
_RS07550
Position (kb) 1427
1428
1429 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1 at 1426.743 kb on - strand, within MMP_RS07530 at 1426.744 kb on + strand, within MMP_RS07530 at 1426.886 kb on - strand at 1426.947 kb on - strand at 1427.029 kb on - strand, within MMP_RS07535 at 1427.053 kb on + strand, within MMP_RS07535 at 1427.228 kb on - strand, within MMP_RS07535 at 1427.229 kb on + strand, within MMP_RS07535 at 1427.256 kb on + strand, within MMP_RS07535 at 1427.380 kb on + strand, within MMP_RS07535 at 1427.381 kb on - strand, within MMP_RS07535 at 1427.381 kb on - strand, within MMP_RS07535 at 1427.382 kb on + strand, within MMP_RS07535 at 1427.423 kb on - strand, within MMP_RS07535 at 1427.517 kb on - strand, within MMP_RS07535 at 1427.523 kb on + strand, within MMP_RS07535 at 1427.534 kb on - strand, within MMP_RS07535 at 1427.586 kb on - strand, within MMP_RS07535 at 1427.586 kb on - strand, within MMP_RS07535 at 1427.586 kb on - strand, within MMP_RS07535 at 1427.587 kb on + strand, within MMP_RS07535 at 1427.587 kb on + strand, within MMP_RS07535 at 1427.663 kb on + strand at 1427.762 kb on + strand, within MMP_RS07540 at 1427.898 kb on + strand, within MMP_RS07540 at 1427.940 kb on - strand, within MMP_RS07540 at 1427.953 kb on - strand, within MMP_RS07540 at 1427.968 kb on + strand, within MMP_RS07540 at 1428.202 kb on + strand, within MMP_RS07540 at 1428.358 kb on - strand at 1428.364 kb on - strand at 1428.483 kb on + strand, within MMP_RS07545 at 1428.566 kb on - strand, within MMP_RS07545 at 1428.777 kb on - strand at 1428.876 kb on - strand, within MMP_RS07550 at 1428.958 kb on + strand, within MMP_RS07550 at 1429.071 kb on - strand, within MMP_RS07550 at 1429.107 kb on + strand, within MMP_RS07550 at 1429.299 kb on + strand, within MMP_RS07550 at 1429.307 kb on - strand, within MMP_RS07550
Per-strain Table
Position Strand Gene LocusTag Fraction Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; 1,3-Propanediol_timecourse1; 20 generations remove 1,426,743 - MMP_RS07530 0.71 -0.6 1,426,744 + MMP_RS07530 0.71 +0.6 1,426,886 - -0.5 1,426,947 - -0.4 1,427,029 - MMP_RS07535 0.16 -1.1 1,427,053 + MMP_RS07535 0.19 -1.7 1,427,228 - MMP_RS07535 0.42 -0.4 1,427,229 + MMP_RS07535 0.43 -0.0 1,427,256 + MMP_RS07535 0.46 -1.4 1,427,380 + MMP_RS07535 0.62 -1.4 1,427,381 - MMP_RS07535 0.63 -1.4 1,427,381 - MMP_RS07535 0.63 -1.1 1,427,382 + MMP_RS07535 0.63 -0.7 1,427,423 - MMP_RS07535 0.68 -0.8 1,427,517 - MMP_RS07535 0.81 -4.5 1,427,523 + MMP_RS07535 0.81 -4.3 1,427,534 - MMP_RS07535 0.83 -3.6 1,427,586 - MMP_RS07535 0.90 -6.0 1,427,586 - MMP_RS07535 0.90 -3.4 1,427,586 - MMP_RS07535 0.90 -5.4 1,427,587 + MMP_RS07535 0.90 -2.7 1,427,587 + MMP_RS07535 0.90 -3.2 1,427,663 + -4.0 1,427,762 + MMP_RS07540 0.13 -3.8 1,427,898 + MMP_RS07540 0.34 -3.4 1,427,940 - MMP_RS07540 0.40 -5.6 1,427,953 - MMP_RS07540 0.43 -4.4 1,427,968 + MMP_RS07540 0.45 -4.4 1,428,202 + MMP_RS07540 0.81 -2.6 1,428,358 - -5.6 1,428,364 - -4.5 1,428,483 + MMP_RS07545 0.36 -4.9 1,428,566 - MMP_RS07545 0.57 -4.7 1,428,777 - -1.5 1,428,876 - MMP_RS07550 0.11 +0.8 1,428,958 + MMP_RS07550 0.17 +0.7 1,429,071 - MMP_RS07550 0.27 +1.0 1,429,107 + MMP_RS07550 0.30 +0.7 1,429,299 + MMP_RS07550 0.46 +0.2 1,429,307 - MMP_RS07550 0.47 +0.4
Or see this region's nucleotide sequence