Experiment: Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; 1,3-Propanediol_timecourse1; 20 generations
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS07415 and MMP_RS07420 are separated by 110 nucleotides MMP_RS07420 and MMP_RS07425 are separated by 195 nucleotides MMP_RS07425 and MMP_RS07430 are separated by 10 nucleotides MMP_RS07430 and MMP_RS07435 are separated by 19 nucleotides
MMP_RS07415: MMP_RS07415 - molybdopterin-guanine dinucleotide biosynthesis protein MobB, at 1,412,750 to 1,413,439
_RS07415
MMP_RS07420: MMP_RS07420 - metalloregulator ArsR/SmtB family transcription factor, at 1,413,550 to 1,413,837
_RS07420
MMP_RS07425: MMP_RS07425 - GTP-binding protein, at 1,414,033 to 1,415,142
_RS07425
MMP_RS07430: MMP_RS07430 - type II methionyl aminopeptidase, at 1,415,153 to 1,416,040
_RS07430
MMP_RS07435: MMP_RS07435 - GMP synthase subunit A, at 1,416,060 to 1,416,629
_RS07435
Position (kb)
1414
1415
1416 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 1413.055 kb on - strand, within MMP_RS07415 at 1413.067 kb on + strand, within MMP_RS07415 at 1413.073 kb on + strand, within MMP_RS07415 at 1413.195 kb on + strand, within MMP_RS07415 at 1413.297 kb on - strand, within MMP_RS07415 at 1413.365 kb on + strand, within MMP_RS07415 at 1413.365 kb on + strand, within MMP_RS07415 at 1413.366 kb on - strand, within MMP_RS07415 at 1413.378 kb on - strand at 1413.379 kb on + strand at 1413.379 kb on + strand at 1413.445 kb on + strand at 1413.475 kb on - strand at 1413.526 kb on + strand at 1413.527 kb on - strand at 1413.557 kb on - strand at 1413.571 kb on - strand at 1413.615 kb on - strand, within MMP_RS07420 at 1413.616 kb on + strand, within MMP_RS07420 at 1413.639 kb on + strand, within MMP_RS07420 at 1413.654 kb on - strand, within MMP_RS07420 at 1413.775 kb on - strand, within MMP_RS07420 at 1413.778 kb on - strand, within MMP_RS07420 at 1413.778 kb on - strand, within MMP_RS07420 at 1413.779 kb on + strand, within MMP_RS07420 at 1413.779 kb on + strand, within MMP_RS07420 at 1413.785 kb on - strand, within MMP_RS07420 at 1413.797 kb on - strand, within MMP_RS07420 at 1413.810 kb on + strand at 1413.813 kb on - strand at 1413.813 kb on - strand at 1413.813 kb on - strand at 1413.814 kb on + strand at 1413.814 kb on + strand at 1413.814 kb on + strand at 1413.814 kb on + strand at 1413.814 kb on + strand at 1413.817 kb on + strand at 1413.875 kb on - strand at 1413.877 kb on - strand at 1413.877 kb on - strand at 1413.877 kb on - strand at 1413.896 kb on - strand at 1413.897 kb on + strand at 1413.897 kb on + strand at 1413.898 kb on - strand at 1413.898 kb on - strand at 1413.899 kb on + strand at 1413.919 kb on + strand at 1414.074 kb on + strand at 1414.076 kb on - strand at 1414.089 kb on - strand at 1414.089 kb on - strand at 1414.090 kb on + strand at 1414.138 kb on + strand at 1414.279 kb on + strand, within MMP_RS07425 at 1414.451 kb on - strand, within MMP_RS07425 at 1414.452 kb on + strand, within MMP_RS07425 at 1414.633 kb on + strand, within MMP_RS07425 at 1414.683 kb on + strand, within MMP_RS07425 at 1414.685 kb on - strand, within MMP_RS07425 at 1414.836 kb on + strand, within MMP_RS07425 at 1414.906 kb on + strand, within MMP_RS07425 at 1414.958 kb on - strand, within MMP_RS07425 at 1414.963 kb on + strand, within MMP_RS07425 at 1414.996 kb on + strand, within MMP_RS07425 at 1415.110 kb on + strand at 1415.112 kb on + strand at 1415.515 kb on + strand at 1415.543 kb on + strand at 1415.652 kb on - strand at 1415.652 kb on - strand at 1415.766 kb on - strand at 1415.771 kb on + strand at 1415.824 kb on + strand at 1415.853 kb on + strand at 1415.996 kb on + strand at 1416.120 kb on - strand, within MMP_RS07435 at 1416.130 kb on - strand, within MMP_RS07435
Per-strain Table
Position Strand Gene LocusTag Fraction Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; 1,3-Propanediol_timecourse1; 20 generations remove 1,413,055 - MMP_RS07415 0.44 -3.3 1,413,067 + MMP_RS07415 0.46 -3.8 1,413,073 + MMP_RS07415 0.47 -3.8 1,413,195 + MMP_RS07415 0.64 -0.7 1,413,297 - MMP_RS07415 0.79 -1.1 1,413,365 + MMP_RS07415 0.89 -4.7 1,413,365 + MMP_RS07415 0.89 -3.0 1,413,366 - MMP_RS07415 0.89 -2.7 1,413,378 - -2.3 1,413,379 + -2.1 1,413,379 + -3.1 1,413,445 + +1.0 1,413,475 - +1.3 1,413,526 + -4.6 1,413,527 - -3.8 1,413,557 - -5.4 1,413,571 - -2.8 1,413,615 - MMP_RS07420 0.23 -3.8 1,413,616 + MMP_RS07420 0.23 -2.7 1,413,639 + MMP_RS07420 0.31 -3.3 1,413,654 - MMP_RS07420 0.36 -5.3 1,413,775 - MMP_RS07420 0.78 -2.8 1,413,778 - MMP_RS07420 0.79 -2.7 1,413,778 - MMP_RS07420 0.79 -2.6 1,413,779 + MMP_RS07420 0.80 -3.1 1,413,779 + MMP_RS07420 0.80 -2.1 1,413,785 - MMP_RS07420 0.82 -0.5 1,413,797 - MMP_RS07420 0.86 +0.2 1,413,810 + +0.1 1,413,813 - +0.0 1,413,813 - -0.1 1,413,813 - -0.5 1,413,814 + -0.6 1,413,814 + -0.5 1,413,814 + -0.5 1,413,814 + -0.2 1,413,814 + -0.4 1,413,817 + +0.1 1,413,875 - +1.3 1,413,877 - +0.4 1,413,877 - +0.2 1,413,877 - +0.6 1,413,896 - -4.5 1,413,897 + -4.4 1,413,897 + -4.3 1,413,898 - -4.9 1,413,898 - -3.7 1,413,899 + -3.7 1,413,919 + -4.6 1,414,074 + -3.7 1,414,076 - -4.7 1,414,089 - -5.4 1,414,089 - -4.1 1,414,090 + -3.5 1,414,138 + -4.2 1,414,279 + MMP_RS07425 0.22 -4.5 1,414,451 - MMP_RS07425 0.38 -4.2 1,414,452 + MMP_RS07425 0.38 -3.9 1,414,633 + MMP_RS07425 0.54 -3.6 1,414,683 + MMP_RS07425 0.59 -3.4 1,414,685 - MMP_RS07425 0.59 -4.5 1,414,836 + MMP_RS07425 0.72 -2.0 1,414,906 + MMP_RS07425 0.79 -2.4 1,414,958 - MMP_RS07425 0.83 -4.5 1,414,963 + MMP_RS07425 0.84 -5.3 1,414,996 + MMP_RS07425 0.87 -3.8 1,415,110 + -3.4 1,415,112 + -3.0 1,415,515 + -3.8 1,415,543 + -4.3 1,415,652 - -5.1 1,415,652 - -3.5 1,415,766 - -4.7 1,415,771 + -2.9 1,415,824 + -3.8 1,415,853 + -4.0 1,415,996 + -5.2 1,416,120 - MMP_RS07435 0.11 -4.2 1,416,130 - MMP_RS07435 0.12 -3.8
Or see this region's nucleotide sequence