Experiment: Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; 1,3-Propanediol_timecourse1; 20 generations
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS06975 and MMP_RS06980 are separated by 90 nucleotides MMP_RS06980 and MMP_RS06985 are separated by 138 nucleotides
MMP_RS06975: MMP_RS06975 - sulfide-dependent adenosine diphosphate thiazole synthase, at 1,331,721 to 1,332,509
_RS06975
MMP_RS06980: MMP_RS06980 - tRNA 2-thiocytidine biosynthesis TtcA family protein, at 1,332,600 to 1,333,574
_RS06980
MMP_RS06985: MMP_RS06985 - tRNA 4-thiouridine(8) synthase ThiI, at 1,333,713 to 1,334,864
_RS06985
Position (kb)
1332
1333
1334 Strain fitness (log2 ratio)
-7
-6
-5
-4
-3
-2
-1
0
1
2 at 1331.600 kb on + strand at 1331.666 kb on - strand at 1331.708 kb on + strand at 1331.776 kb on - strand at 1331.876 kb on - strand, within MMP_RS06975 at 1332.005 kb on + strand, within MMP_RS06975 at 1332.005 kb on + strand, within MMP_RS06975 at 1332.545 kb on + strand at 1332.613 kb on - strand at 1332.613 kb on - strand at 1332.725 kb on - strand, within MMP_RS06980 at 1332.726 kb on + strand, within MMP_RS06980 at 1332.761 kb on + strand, within MMP_RS06980 at 1332.873 kb on - strand, within MMP_RS06980 at 1332.874 kb on + strand, within MMP_RS06980 at 1332.916 kb on + strand, within MMP_RS06980 at 1332.919 kb on + strand, within MMP_RS06980 at 1332.920 kb on - strand, within MMP_RS06980 at 1332.934 kb on + strand, within MMP_RS06980 at 1332.947 kb on - strand, within MMP_RS06980 at 1332.947 kb on - strand, within MMP_RS06980 at 1333.237 kb on + strand, within MMP_RS06980 at 1333.362 kb on - strand, within MMP_RS06980 at 1333.506 kb on + strand at 1333.528 kb on + strand at 1333.532 kb on + strand at 1333.563 kb on - strand at 1333.564 kb on + strand at 1333.590 kb on - strand at 1333.602 kb on + strand at 1333.602 kb on + strand at 1333.602 kb on + strand at 1333.602 kb on + strand at 1333.605 kb on - strand at 1333.605 kb on - strand at 1333.606 kb on + strand at 1333.606 kb on + strand at 1333.606 kb on + strand at 1333.608 kb on + strand at 1333.615 kb on - strand at 1333.615 kb on - strand at 1333.615 kb on - strand at 1333.617 kb on - strand at 1333.618 kb on + strand at 1333.619 kb on - strand at 1333.619 kb on - strand at 1333.620 kb on + strand at 1333.620 kb on + strand at 1333.627 kb on + strand at 1333.645 kb on + strand at 1333.649 kb on + strand at 1333.655 kb on - strand at 1333.656 kb on + strand at 1333.656 kb on + strand at 1333.660 kb on + strand at 1333.660 kb on + strand at 1333.668 kb on - strand at 1333.679 kb on + strand at 1333.679 kb on + strand at 1333.948 kb on + strand at 1334.046 kb on + strand at 1334.330 kb on - strand at 1334.345 kb on + strand at 1334.434 kb on + strand at 1334.434 kb on + strand at 1334.445 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; 1,3-Propanediol_timecourse1; 20 generations remove 1,331,600 + +0.0 1,331,666 - +1.0 1,331,708 + +0.1 1,331,776 - +1.5 1,331,876 - MMP_RS06975 0.20 +1.3 1,332,005 + MMP_RS06975 0.36 +1.4 1,332,005 + MMP_RS06975 0.36 +0.9 1,332,545 + +1.7 1,332,613 - +1.5 1,332,613 - +0.9 1,332,725 - MMP_RS06980 0.13 -5.0 1,332,726 + MMP_RS06980 0.13 -4.7 1,332,761 + MMP_RS06980 0.17 -5.8 1,332,873 - MMP_RS06980 0.28 -6.2 1,332,874 + MMP_RS06980 0.28 -5.7 1,332,916 + MMP_RS06980 0.32 -4.8 1,332,919 + MMP_RS06980 0.33 -6.3 1,332,920 - MMP_RS06980 0.33 -7.4 1,332,934 + MMP_RS06980 0.34 -4.9 1,332,947 - MMP_RS06980 0.36 -5.9 1,332,947 - MMP_RS06980 0.36 -6.5 1,333,237 + MMP_RS06980 0.65 -4.8 1,333,362 - MMP_RS06980 0.78 -7.5 1,333,506 + -5.9 1,333,528 + -5.8 1,333,532 + -5.7 1,333,563 - -6.1 1,333,564 + -3.9 1,333,590 - +0.2 1,333,602 + -0.2 1,333,602 + -1.0 1,333,602 + +0.2 1,333,602 + +0.2 1,333,605 - +0.2 1,333,605 - -0.1 1,333,606 + -0.3 1,333,606 + -0.4 1,333,606 + -0.8 1,333,608 + +0.0 1,333,615 - +0.4 1,333,615 - -0.1 1,333,615 - +0.6 1,333,617 - +0.2 1,333,618 + +0.6 1,333,619 - +2.4 1,333,619 - +0.6 1,333,620 + -0.3 1,333,620 + +0.1 1,333,627 + -0.3 1,333,645 + -0.3 1,333,649 + -0.1 1,333,655 - -3.0 1,333,656 + -0.3 1,333,656 + -0.2 1,333,660 + +0.5 1,333,660 + -0.2 1,333,668 - -4.3 1,333,679 + -1.4 1,333,679 + -0.9 1,333,948 + -3.8 1,334,046 + -4.2 1,334,330 - -4.0 1,334,345 + -3.2 1,334,434 + -4.0 1,334,434 + -4.7 1,334,445 + -5.4
Or see this region's nucleotide sequence