Experiment: Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; 1,3-Propanediol_timecourse1; 20 generations
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS06765 and MMP_RS06770 are separated by 36 nucleotides MMP_RS06770 and MMP_RS06775 are separated by 320 nucleotides MMP_RS06775 and MMP_RS06780 are separated by 9 nucleotides
MMP_RS06765: MMP_RS06765 - flap endonuclease-1, at 1,297,500 to 1,298,474
_RS06765
MMP_RS06770: MMP_RS06770 - DHH family phosphoesterase, at 1,298,511 to 1,300,796
_RS06770
MMP_RS06775: MMP_RS06775 - 2-oxoacid:ferredoxin oxidoreductase subunit gamma, at 1,301,117 to 1,301,650
_RS06775
MMP_RS06780: MMP_RS06780 - 2-oxoacid:ferredoxin oxidoreductase subunit beta, at 1,301,660 to 1,302,457
_RS06780
Position (kb)
1298
1299
1300
1301 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1 at 1297.650 kb on - strand, within MMP_RS06765 at 1297.706 kb on + strand, within MMP_RS06765 at 1297.965 kb on - strand, within MMP_RS06765 at 1297.966 kb on + strand, within MMP_RS06765 at 1297.966 kb on + strand, within MMP_RS06765 at 1297.967 kb on - strand, within MMP_RS06765 at 1298.034 kb on - strand, within MMP_RS06765 at 1298.034 kb on - strand, within MMP_RS06765 at 1298.035 kb on + strand, within MMP_RS06765 at 1298.038 kb on - strand, within MMP_RS06765 at 1298.411 kb on - strand at 1298.661 kb on - strand at 1298.661 kb on - strand at 1298.661 kb on - strand at 1298.661 kb on - strand at 1298.662 kb on + strand at 1298.662 kb on + strand at 1298.662 kb on + strand at 1298.663 kb on - strand at 1299.163 kb on - strand, within MMP_RS06770 at 1299.249 kb on - strand, within MMP_RS06770 at 1299.546 kb on - strand, within MMP_RS06770 at 1299.581 kb on - strand, within MMP_RS06770 at 1299.581 kb on - strand, within MMP_RS06770 at 1299.775 kb on + strand, within MMP_RS06770 at 1299.841 kb on + strand, within MMP_RS06770 at 1299.901 kb on + strand, within MMP_RS06770 at 1299.980 kb on - strand, within MMP_RS06770 at 1299.981 kb on + strand, within MMP_RS06770 at 1300.091 kb on - strand, within MMP_RS06770 at 1300.100 kb on - strand, within MMP_RS06770 at 1300.110 kb on + strand, within MMP_RS06770 at 1300.150 kb on + strand, within MMP_RS06770 at 1300.461 kb on - strand, within MMP_RS06770 at 1300.623 kb on + strand at 1300.797 kb on + strand at 1300.799 kb on - strand at 1300.836 kb on + strand at 1300.878 kb on - strand at 1300.879 kb on + strand at 1300.881 kb on + strand at 1300.882 kb on - strand at 1300.884 kb on - strand at 1300.884 kb on - strand at 1300.958 kb on + strand at 1300.960 kb on + strand at 1301.108 kb on - strand at 1301.444 kb on + strand at 1301.588 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; 1,3-Propanediol_timecourse1; 20 generations remove 1,297,650 - MMP_RS06765 0.15 -0.5 1,297,706 + MMP_RS06765 0.21 -1.1 1,297,965 - MMP_RS06765 0.48 -0.1 1,297,966 + MMP_RS06765 0.48 -2.4 1,297,966 + MMP_RS06765 0.48 -1.3 1,297,967 - MMP_RS06765 0.48 +0.3 1,298,034 - MMP_RS06765 0.55 -0.4 1,298,034 - MMP_RS06765 0.55 -0.6 1,298,035 + MMP_RS06765 0.55 -0.6 1,298,038 - MMP_RS06765 0.55 +0.1 1,298,411 - -0.3 1,298,661 - -6.0 1,298,661 - -4.5 1,298,661 - -4.5 1,298,661 - -5.0 1,298,662 + -6.6 1,298,662 + -4.4 1,298,662 + -3.8 1,298,663 - -5.2 1,299,163 - MMP_RS06770 0.29 -5.1 1,299,249 - MMP_RS06770 0.32 -4.5 1,299,546 - MMP_RS06770 0.45 -4.2 1,299,581 - MMP_RS06770 0.47 -4.1 1,299,581 - MMP_RS06770 0.47 -2.4 1,299,775 + MMP_RS06770 0.55 -5.1 1,299,841 + MMP_RS06770 0.58 -4.8 1,299,901 + MMP_RS06770 0.61 -2.6 1,299,980 - MMP_RS06770 0.64 -5.8 1,299,981 + MMP_RS06770 0.64 -4.2 1,300,091 - MMP_RS06770 0.69 -3.9 1,300,100 - MMP_RS06770 0.70 -6.6 1,300,110 + MMP_RS06770 0.70 -5.2 1,300,150 + MMP_RS06770 0.72 -3.1 1,300,461 - MMP_RS06770 0.85 -4.9 1,300,623 + -4.8 1,300,797 + -4.2 1,300,799 - -5.5 1,300,836 + -3.4 1,300,878 - -1.4 1,300,879 + -5.6 1,300,881 + -2.0 1,300,882 - +0.3 1,300,884 - +0.5 1,300,884 - +0.7 1,300,958 + +0.1 1,300,960 + +0.1 1,301,108 - +0.5 1,301,444 + -3.0 1,301,588 - -3.2
Or see this region's nucleotide sequence