Experiment: Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; 1,3-Propanediol_timecourse1; 20 generations
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS06100 and MMP_RS06105 are separated by 127 nucleotides MMP_RS06105 and MMP_RS06110 are separated by 12 nucleotides MMP_RS06110 and MMP_RS06115 are separated by 30 nucleotides MMP_RS06115 and MMP_RS06120 are separated by 71 nucleotides
MMP_RS06100: MMP_RS06100 - GNAT family N-acetyltransferase, at 1,167,389 to 1,167,907
_RS06100
MMP_RS06105: MMP_RS06105 - hydrogenase maturation peptidase HycI, at 1,168,035 to 1,168,574
_RS06105
MMP_RS06110: MMP_RS06110 - ATP-dependent protease LonB, at 1,168,587 to 1,170,665
_RS06110
MMP_RS06115: MMP_RS06115 - class II aldolase/adducin family protein, at 1,170,696 to 1,171,238
_RS06115
MMP_RS06120: MMP_RS06120 - UPF0147 family protein, at 1,171,310 to 1,171,585
_RS06120
Position (kb)
1168
1169
1170
1171 Strain fitness (log2 ratio)
-7
-6
-5
-4
-3
-2
-1
0
1 at 1167.635 kb on + strand, within MMP_RS06100 at 1167.635 kb on + strand, within MMP_RS06100 at 1167.635 kb on + strand, within MMP_RS06100 at 1167.636 kb on - strand, within MMP_RS06100 at 1167.637 kb on + strand, within MMP_RS06100 at 1167.638 kb on - strand, within MMP_RS06100 at 1167.639 kb on + strand, within MMP_RS06100 at 1167.653 kb on + strand, within MMP_RS06100 at 1167.707 kb on + strand, within MMP_RS06100 at 1167.740 kb on + strand, within MMP_RS06100 at 1167.741 kb on - strand, within MMP_RS06100 at 1167.742 kb on + strand, within MMP_RS06100 at 1167.833 kb on + strand, within MMP_RS06100 at 1167.841 kb on - strand, within MMP_RS06100 at 1167.842 kb on + strand, within MMP_RS06100 at 1167.852 kb on - strand, within MMP_RS06100 at 1167.857 kb on - strand at 1167.857 kb on - strand at 1167.857 kb on - strand at 1167.889 kb on - strand at 1167.929 kb on + strand at 1167.961 kb on + strand at 1167.962 kb on - strand at 1167.964 kb on - strand at 1167.964 kb on - strand at 1167.964 kb on - strand at 1167.965 kb on + strand at 1167.965 kb on + strand at 1167.966 kb on - strand at 1167.966 kb on - strand at 1167.966 kb on - strand at 1167.967 kb on + strand at 1167.974 kb on - strand at 1167.975 kb on + strand at 1167.986 kb on - strand at 1167.989 kb on - strand at 1167.989 kb on - strand at 1167.990 kb on + strand at 1167.990 kb on + strand at 1167.991 kb on - strand at 1167.991 kb on - strand at 1167.994 kb on + strand at 1167.994 kb on + strand at 1167.996 kb on + strand at 1167.996 kb on + strand at 1167.996 kb on + strand at 1167.996 kb on + strand at 1167.996 kb on + strand at 1168.053 kb on + strand at 1168.059 kb on + strand at 1168.067 kb on - strand at 1168.097 kb on + strand, within MMP_RS06105 at 1168.097 kb on + strand, within MMP_RS06105 at 1168.097 kb on + strand, within MMP_RS06105 at 1168.101 kb on + strand, within MMP_RS06105 at 1168.141 kb on + strand, within MMP_RS06105 at 1168.216 kb on - strand, within MMP_RS06105 at 1168.220 kb on - strand, within MMP_RS06105 at 1168.228 kb on - strand, within MMP_RS06105 at 1168.249 kb on - strand, within MMP_RS06105 at 1168.250 kb on + strand, within MMP_RS06105 at 1168.322 kb on + strand, within MMP_RS06105 at 1168.412 kb on + strand, within MMP_RS06105 at 1168.415 kb on + strand, within MMP_RS06105 at 1168.419 kb on + strand, within MMP_RS06105 at 1168.422 kb on - strand, within MMP_RS06105 at 1168.454 kb on + strand, within MMP_RS06105 at 1168.458 kb on + strand, within MMP_RS06105 at 1168.465 kb on - strand, within MMP_RS06105 at 1168.586 kb on - strand at 1168.803 kb on - strand, within MMP_RS06110 at 1168.849 kb on - strand, within MMP_RS06110 at 1169.141 kb on + strand, within MMP_RS06110 at 1169.362 kb on - strand, within MMP_RS06110 at 1169.962 kb on - strand, within MMP_RS06110 at 1169.990 kb on - strand, within MMP_RS06110 at 1169.993 kb on + strand, within MMP_RS06110 at 1170.025 kb on - strand, within MMP_RS06110 at 1170.200 kb on - strand, within MMP_RS06110 at 1170.662 kb on + strand at 1170.669 kb on - strand at 1170.672 kb on - strand at 1170.713 kb on - strand at 1170.735 kb on - strand at 1170.773 kb on + strand, within MMP_RS06115 at 1170.797 kb on + strand, within MMP_RS06115 at 1170.798 kb on - strand, within MMP_RS06115 at 1170.799 kb on + strand, within MMP_RS06115 at 1170.799 kb on + strand, within MMP_RS06115 at 1170.799 kb on + strand, within MMP_RS06115 at 1170.799 kb on + strand, within MMP_RS06115 at 1170.799 kb on + strand, within MMP_RS06115 at 1170.799 kb on + strand, within MMP_RS06115 at 1170.799 kb on + strand, within MMP_RS06115 at 1170.800 kb on - strand, within MMP_RS06115 at 1170.801 kb on + strand, within MMP_RS06115 at 1170.801 kb on + strand, within MMP_RS06115 at 1170.801 kb on + strand, within MMP_RS06115 at 1170.803 kb on - strand, within MMP_RS06115 at 1170.846 kb on + strand, within MMP_RS06115 at 1170.846 kb on + strand, within MMP_RS06115 at 1170.878 kb on + strand, within MMP_RS06115 at 1170.878 kb on + strand, within MMP_RS06115 at 1170.891 kb on + strand, within MMP_RS06115 at 1170.950 kb on + strand, within MMP_RS06115 at 1171.051 kb on + strand, within MMP_RS06115 at 1171.051 kb on + strand, within MMP_RS06115 at 1171.173 kb on - strand, within MMP_RS06115 at 1171.174 kb on + strand, within MMP_RS06115 at 1171.211 kb on + strand at 1171.215 kb on - strand at 1171.216 kb on + strand at 1171.220 kb on - strand at 1171.220 kb on - strand at 1171.221 kb on + strand at 1171.221 kb on + strand at 1171.232 kb on + strand at 1171.242 kb on + strand at 1171.255 kb on + strand at 1171.307 kb on - strand at 1171.337 kb on - strand at 1171.338 kb on + strand, within MMP_RS06120 at 1171.338 kb on + strand, within MMP_RS06120 at 1171.375 kb on - strand, within MMP_RS06120 at 1171.541 kb on + strand, within MMP_RS06120 at 1171.647 kb on - strand at 1171.654 kb on - strand at 1171.654 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; 1,3-Propanediol_timecourse1; 20 generations remove 1,167,635 + MMP_RS06100 0.47 +0.1 1,167,635 + MMP_RS06100 0.47 +0.0 1,167,635 + MMP_RS06100 0.47 +0.3 1,167,636 - MMP_RS06100 0.48 +0.2 1,167,637 + MMP_RS06100 0.48 +0.9 1,167,638 - MMP_RS06100 0.48 +0.3 1,167,639 + MMP_RS06100 0.48 -0.3 1,167,653 + MMP_RS06100 0.51 +0.4 1,167,707 + MMP_RS06100 0.61 +0.4 1,167,740 + MMP_RS06100 0.68 +0.3 1,167,741 - MMP_RS06100 0.68 +0.3 1,167,742 + MMP_RS06100 0.68 +0.4 1,167,833 + MMP_RS06100 0.86 +0.5 1,167,841 - MMP_RS06100 0.87 +0.3 1,167,842 + MMP_RS06100 0.87 +0.6 1,167,852 - MMP_RS06100 0.89 -0.2 1,167,857 - -0.1 1,167,857 - +0.3 1,167,857 - +0.5 1,167,889 - -0.0 1,167,929 + -0.4 1,167,961 + +0.6 1,167,962 - +0.3 1,167,964 - -6.8 1,167,964 - -6.8 1,167,964 - -5.3 1,167,965 + -3.4 1,167,965 + -3.3 1,167,966 - -4.5 1,167,966 - -6.2 1,167,966 - -6.2 1,167,967 + -4.4 1,167,974 - -6.1 1,167,975 + -5.4 1,167,986 - -2.7 1,167,989 - -6.1 1,167,989 - -5.3 1,167,990 + -4.6 1,167,990 + -6.3 1,167,991 - -4.4 1,167,991 - -5.7 1,167,994 + -2.2 1,167,994 + -4.0 1,167,996 + -3.3 1,167,996 + -4.5 1,167,996 + -6.0 1,167,996 + -3.7 1,167,996 + -4.0 1,168,053 + -5.0 1,168,059 + -5.8 1,168,067 - -6.2 1,168,097 + MMP_RS06105 0.11 -7.2 1,168,097 + MMP_RS06105 0.11 -7.3 1,168,097 + MMP_RS06105 0.11 -5.0 1,168,101 + MMP_RS06105 0.12 -6.4 1,168,141 + MMP_RS06105 0.20 -5.9 1,168,216 - MMP_RS06105 0.34 -6.0 1,168,220 - MMP_RS06105 0.34 -5.2 1,168,228 - MMP_RS06105 0.36 -6.0 1,168,249 - MMP_RS06105 0.40 -3.4 1,168,250 + MMP_RS06105 0.40 -6.1 1,168,322 + MMP_RS06105 0.53 -6.6 1,168,412 + MMP_RS06105 0.70 -6.2 1,168,415 + MMP_RS06105 0.70 -4.9 1,168,419 + MMP_RS06105 0.71 -3.8 1,168,422 - MMP_RS06105 0.72 -2.9 1,168,454 + MMP_RS06105 0.78 -4.8 1,168,458 + MMP_RS06105 0.78 -5.2 1,168,465 - MMP_RS06105 0.80 -4.8 1,168,586 - -2.8 1,168,803 - MMP_RS06110 0.10 -2.4 1,168,849 - MMP_RS06110 0.13 -5.0 1,169,141 + MMP_RS06110 0.27 -3.1 1,169,362 - MMP_RS06110 0.37 -3.6 1,169,962 - MMP_RS06110 0.66 -3.0 1,169,990 - MMP_RS06110 0.67 -3.1 1,169,993 + MMP_RS06110 0.68 -3.7 1,170,025 - MMP_RS06110 0.69 -3.4 1,170,200 - MMP_RS06110 0.78 -3.9 1,170,662 + +0.1 1,170,669 - -0.3 1,170,672 - +0.4 1,170,713 - +0.0 1,170,735 - +0.4 1,170,773 + MMP_RS06115 0.14 -0.3 1,170,797 + MMP_RS06115 0.19 +0.8 1,170,798 - MMP_RS06115 0.19 +0.5 1,170,799 + MMP_RS06115 0.19 +0.2 1,170,799 + MMP_RS06115 0.19 -0.4 1,170,799 + MMP_RS06115 0.19 -0.4 1,170,799 + MMP_RS06115 0.19 -0.1 1,170,799 + MMP_RS06115 0.19 +0.4 1,170,799 + MMP_RS06115 0.19 +0.9 1,170,799 + MMP_RS06115 0.19 -0.1 1,170,800 - MMP_RS06115 0.19 +1.0 1,170,801 + MMP_RS06115 0.19 +0.6 1,170,801 + MMP_RS06115 0.19 +0.3 1,170,801 + MMP_RS06115 0.19 +0.2 1,170,803 - MMP_RS06115 0.20 +0.4 1,170,846 + MMP_RS06115 0.28 +0.4 1,170,846 + MMP_RS06115 0.28 +0.4 1,170,878 + MMP_RS06115 0.34 +0.2 1,170,878 + MMP_RS06115 0.34 +0.0 1,170,891 + MMP_RS06115 0.36 +0.3 1,170,950 + MMP_RS06115 0.47 +0.8 1,171,051 + MMP_RS06115 0.65 +0.3 1,171,051 + MMP_RS06115 0.65 +0.1 1,171,173 - MMP_RS06115 0.88 +0.6 1,171,174 + MMP_RS06115 0.88 -0.2 1,171,211 + +0.7 1,171,215 - +0.2 1,171,216 + +0.4 1,171,220 - +0.5 1,171,220 - +0.3 1,171,221 + +0.7 1,171,221 + -0.0 1,171,232 + +0.7 1,171,242 + +1.0 1,171,255 + +0.1 1,171,307 - +0.9 1,171,337 - -4.2 1,171,338 + MMP_RS06120 0.10 -0.8 1,171,338 + MMP_RS06120 0.10 -4.9 1,171,375 - MMP_RS06120 0.24 -5.1 1,171,541 + MMP_RS06120 0.84 -4.4 1,171,647 - -1.6 1,171,654 - -5.4 1,171,654 - -6.0
Or see this region's nucleotide sequence