Experiment: Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; 1,3-Propanediol_timecourse1; 20 generations
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS05505 and MMP_RS05510 overlap by 4 nucleotides MMP_RS05510 and MMP_RS05515 are separated by 109 nucleotides MMP_RS05515 and MMP_RS05520 are separated by 18 nucleotides
MMP_RS05505: MMP_RS05505 - class IV adenylate cyclase, at 1,057,240 to 1,057,803
_RS05505
MMP_RS05510: MMP_RS05510 - Rrf2 family transcriptional regulator, at 1,057,800 to 1,058,234
_RS05510
MMP_RS05515: MMP_RS05515 - cyclic pyranopterin monophosphate synthase MoaC, at 1,058,344 to 1,058,805
_RS05515
MMP_RS05520: MMP_RS05520 - succinate dehydrogenase/fumarate reductase iron-sulfur subunit, at 1,058,824 to 1,060,308
_RS05520
Position (kb)
1058
1059 Strain fitness (log2 ratio)
-8
-7
-6
-5
-4
-3
-2
-1
0
1 at 1057.509 kb on + strand, within MMP_RS05505 at 1057.590 kb on - strand, within MMP_RS05505 at 1057.595 kb on - strand, within MMP_RS05505 at 1057.595 kb on - strand, within MMP_RS05505 at 1057.595 kb on - strand, within MMP_RS05505 at 1057.595 kb on - strand, within MMP_RS05505 at 1057.596 kb on + strand, within MMP_RS05505 at 1057.596 kb on + strand, within MMP_RS05505 at 1057.675 kb on - strand, within MMP_RS05505 at 1057.676 kb on + strand, within MMP_RS05505 at 1057.689 kb on + strand, within MMP_RS05505 at 1057.787 kb on + strand at 1058.125 kb on - strand, within MMP_RS05510 at 1058.125 kb on - strand, within MMP_RS05510 at 1058.126 kb on + strand, within MMP_RS05510 at 1058.126 kb on + strand, within MMP_RS05510 at 1058.129 kb on - strand, within MMP_RS05510 at 1058.130 kb on + strand, within MMP_RS05510 at 1058.193 kb on + strand at 1058.202 kb on + strand at 1058.264 kb on - strand at 1058.282 kb on - strand at 1058.286 kb on - strand at 1058.286 kb on - strand at 1058.310 kb on - strand at 1058.314 kb on - strand at 1058.314 kb on - strand at 1058.319 kb on - strand at 1058.349 kb on - strand at 1058.434 kb on + strand, within MMP_RS05515 at 1058.482 kb on + strand, within MMP_RS05515 at 1058.533 kb on + strand, within MMP_RS05515 at 1058.619 kb on + strand, within MMP_RS05515 at 1058.648 kb on + strand, within MMP_RS05515 at 1058.648 kb on + strand, within MMP_RS05515 at 1058.718 kb on + strand, within MMP_RS05515 at 1058.973 kb on + strand at 1058.998 kb on - strand at 1059.177 kb on - strand at 1059.192 kb on + strand at 1059.236 kb on + strand at 1059.257 kb on - strand at 1059.424 kb on - strand at 1059.543 kb on + strand at 1059.697 kb on + strand at 1059.697 kb on + strand at 1059.741 kb on - strand at 1059.749 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; 1,3-Propanediol_timecourse1; 20 generations remove 1,057,509 + MMP_RS05505 0.48 -1.0 1,057,590 - MMP_RS05505 0.62 +0.5 1,057,595 - MMP_RS05505 0.63 -0.1 1,057,595 - MMP_RS05505 0.63 +0.0 1,057,595 - MMP_RS05505 0.63 +0.4 1,057,595 - MMP_RS05505 0.63 +0.6 1,057,596 + MMP_RS05505 0.63 -0.1 1,057,596 + MMP_RS05505 0.63 +0.6 1,057,675 - MMP_RS05505 0.77 +0.1 1,057,676 + MMP_RS05505 0.77 +0.4 1,057,689 + MMP_RS05505 0.80 +0.1 1,057,787 + -0.2 1,058,125 - MMP_RS05510 0.75 +0.5 1,058,125 - MMP_RS05510 0.75 +0.0 1,058,126 + MMP_RS05510 0.75 -0.1 1,058,126 + MMP_RS05510 0.75 +0.4 1,058,129 - MMP_RS05510 0.76 -0.0 1,058,130 + MMP_RS05510 0.76 -0.2 1,058,193 + +0.1 1,058,202 + -0.0 1,058,264 - -0.7 1,058,282 - -5.9 1,058,286 - -6.1 1,058,286 - -7.4 1,058,310 - -6.3 1,058,314 - -4.3 1,058,314 - -6.6 1,058,319 - -4.5 1,058,349 - -5.8 1,058,434 + MMP_RS05515 0.19 -7.7 1,058,482 + MMP_RS05515 0.30 -7.8 1,058,533 + MMP_RS05515 0.41 -8.1 1,058,619 + MMP_RS05515 0.60 -6.6 1,058,648 + MMP_RS05515 0.66 -6.4 1,058,648 + MMP_RS05515 0.66 -7.5 1,058,718 + MMP_RS05515 0.81 -6.0 1,058,973 + -4.4 1,058,998 - -4.2 1,059,177 - -5.2 1,059,192 + -3.7 1,059,236 + -3.8 1,059,257 - -3.7 1,059,424 - -2.4 1,059,543 + -3.9 1,059,697 + -4.2 1,059,697 + -4.0 1,059,741 - -3.9 1,059,749 - -3.3
Or see this region's nucleotide sequence