Experiment: Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; 1,3-Propanediol_timecourse1; 20 generations
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS05340 and MMP_RS05345 are separated by 162 nucleotides MMP_RS05345 and MMP_RS05350 are separated by 21 nucleotides MMP_RS05350 and MMP_RS05355 are separated by 71 nucleotides
MMP_RS05340: MMP_RS05340 - OB-fold nucleic acid binding domain-containing protein, at 1,023,461 to 1,025,389
_RS05340
MMP_RS05345: MMP_RS05345 - hypothetical protein, at 1,025,552 to 1,028,482
_RS05345
MMP_RS05350: MMP_RS05350 - dTMP kinase, at 1,028,504 to 1,029,103
_RS05350
MMP_RS05355: MMP_RS05355 - hypothetical protein, at 1,029,175 to 1,029,588
_RS05355
Position (kb)
1025
1026
1027
1028
1029 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1 at 1024.661 kb on - strand at 1024.725 kb on + strand at 1024.727 kb on + strand at 1024.727 kb on + strand at 1024.735 kb on - strand at 1024.736 kb on + strand at 1024.736 kb on + strand at 1025.244 kb on + strand at 1025.419 kb on + strand at 1025.429 kb on + strand at 1025.441 kb on - strand at 1025.442 kb on + strand at 1025.442 kb on + strand at 1025.445 kb on - strand at 1025.452 kb on - strand at 1025.457 kb on - strand at 1025.457 kb on - strand at 1025.492 kb on + strand at 1025.493 kb on - strand at 1025.493 kb on - strand at 1025.493 kb on - strand at 1025.493 kb on - strand at 1025.493 kb on - strand at 1025.494 kb on + strand at 1025.494 kb on + strand at 1025.494 kb on + strand at 1025.494 kb on + strand at 1025.496 kb on - strand at 1025.497 kb on + strand at 1025.497 kb on + strand at 1025.518 kb on - strand at 1025.520 kb on - strand at 1025.521 kb on + strand at 1025.522 kb on - strand at 1025.523 kb on + strand at 1025.560 kb on - strand at 1025.572 kb on + strand at 1025.664 kb on + strand at 1025.668 kb on + strand at 1025.744 kb on + strand at 1025.883 kb on + strand, within MMP_RS05345 at 1025.887 kb on - strand, within MMP_RS05345 at 1025.888 kb on + strand, within MMP_RS05345 at 1025.944 kb on - strand, within MMP_RS05345 at 1025.945 kb on + strand, within MMP_RS05345 at 1026.121 kb on + strand, within MMP_RS05345 at 1026.122 kb on - strand, within MMP_RS05345 at 1026.122 kb on - strand, within MMP_RS05345 at 1026.150 kb on + strand, within MMP_RS05345 at 1026.451 kb on + strand, within MMP_RS05345 at 1026.686 kb on + strand, within MMP_RS05345 at 1027.036 kb on + strand, within MMP_RS05345 at 1027.275 kb on + strand, within MMP_RS05345 at 1027.275 kb on + strand, within MMP_RS05345 at 1027.341 kb on + strand, within MMP_RS05345 at 1027.349 kb on - strand, within MMP_RS05345 at 1027.360 kb on - strand, within MMP_RS05345 at 1027.502 kb on + strand, within MMP_RS05345 at 1027.680 kb on - strand, within MMP_RS05345 at 1027.783 kb on - strand, within MMP_RS05345 at 1027.810 kb on - strand, within MMP_RS05345 at 1027.810 kb on - strand, within MMP_RS05345 at 1027.883 kb on + strand, within MMP_RS05345 at 1028.106 kb on - strand, within MMP_RS05345 at 1028.106 kb on - strand, within MMP_RS05345 at 1028.176 kb on + strand, within MMP_RS05345 at 1028.330 kb on + strand at 1029.092 kb on + strand at 1029.096 kb on - strand at 1029.106 kb on - strand at 1029.107 kb on + strand at 1029.110 kb on - strand at 1029.110 kb on - strand at 1029.111 kb on + strand at 1029.111 kb on + strand at 1029.120 kb on + strand at 1029.127 kb on + strand at 1029.131 kb on - strand at 1029.143 kb on - strand at 1029.144 kb on + strand at 1029.144 kb on + strand at 1029.148 kb on + strand at 1029.148 kb on + strand at 1029.182 kb on - strand at 1029.183 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; 1,3-Propanediol_timecourse1; 20 generations remove 1,024,661 - -5.0 1,024,725 + -3.7 1,024,727 + -2.6 1,024,727 + -3.1 1,024,735 - -3.6 1,024,736 + -2.6 1,024,736 + -2.7 1,025,244 + -3.6 1,025,419 + -4.2 1,025,429 + -4.0 1,025,441 - -4.8 1,025,442 + -3.2 1,025,442 + -3.9 1,025,445 - -4.0 1,025,452 - -1.1 1,025,457 - -1.9 1,025,457 - -0.7 1,025,492 + -3.4 1,025,493 - -5.5 1,025,493 - -3.3 1,025,493 - -3.2 1,025,493 - -6.1 1,025,493 - -5.9 1,025,494 + -1.2 1,025,494 + -1.0 1,025,494 + -3.5 1,025,494 + -0.8 1,025,496 - -4.4 1,025,497 + -5.7 1,025,497 + -4.2 1,025,518 - -4.9 1,025,520 - -3.9 1,025,521 + -4.6 1,025,522 - -4.6 1,025,523 + -4.9 1,025,560 - -3.4 1,025,572 + -3.4 1,025,664 + -4.4 1,025,668 + -2.8 1,025,744 + -2.8 1,025,883 + MMP_RS05345 0.11 -4.0 1,025,887 - MMP_RS05345 0.11 -4.2 1,025,888 + MMP_RS05345 0.11 -3.9 1,025,944 - MMP_RS05345 0.13 -3.5 1,025,945 + MMP_RS05345 0.13 -3.6 1,026,121 + MMP_RS05345 0.19 -4.4 1,026,122 - MMP_RS05345 0.19 -3.8 1,026,122 - MMP_RS05345 0.19 -2.8 1,026,150 + MMP_RS05345 0.20 -5.1 1,026,451 + MMP_RS05345 0.31 -3.9 1,026,686 + MMP_RS05345 0.39 -4.0 1,027,036 + MMP_RS05345 0.51 -3.0 1,027,275 + MMP_RS05345 0.59 -4.9 1,027,275 + MMP_RS05345 0.59 -3.6 1,027,341 + MMP_RS05345 0.61 -3.7 1,027,349 - MMP_RS05345 0.61 -2.1 1,027,360 - MMP_RS05345 0.62 -2.4 1,027,502 + MMP_RS05345 0.67 -3.4 1,027,680 - MMP_RS05345 0.73 -3.5 1,027,783 - MMP_RS05345 0.76 -4.5 1,027,810 - MMP_RS05345 0.77 -4.5 1,027,810 - MMP_RS05345 0.77 -4.3 1,027,883 + MMP_RS05345 0.80 -5.6 1,028,106 - MMP_RS05345 0.87 -3.3 1,028,106 - MMP_RS05345 0.87 -1.7 1,028,176 + MMP_RS05345 0.90 -4.8 1,028,330 + -5.9 1,029,092 + +0.4 1,029,096 - +0.4 1,029,106 - +0.7 1,029,107 + -0.5 1,029,110 - -0.5 1,029,110 - +0.5 1,029,111 + +0.1 1,029,111 + -0.0 1,029,120 + -0.6 1,029,127 + -0.0 1,029,131 - +0.7 1,029,143 - +0.7 1,029,144 + -0.1 1,029,144 + +0.2 1,029,148 + +0.5 1,029,148 + -0.5 1,029,182 - -0.4 1,029,183 + -0.2
Or see this region's nucleotide sequence