Experiment: Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; 1,3-Propanediol_timecourse1; 20 generations
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS05280 and MMP_RS05285 overlap by 8 nucleotides MMP_RS05285 and MMP_RS05290 are separated by 83 nucleotides MMP_RS05290 and MMP_RS05295 are separated by 111 nucleotides
MMP_RS05280: MMP_RS05280 - COG1361 S-layer family protein, at 1,009,427 to 1,010,998
_RS05280
MMP_RS05285: MMP_RS05285 - MMPL family transporter, at 1,010,991 to 1,012,157
_RS05285
MMP_RS05290: MMP_RS05290 - TetR/AcrR family transcriptional regulator, at 1,012,241 to 1,012,843
_RS05290
MMP_RS05295: MMP_RS05295 - minichromosome maintenance protein MCM, at 1,012,955 to 1,015,087
_RS05295
Position (kb)
1010
1011
1012
1013 Strain fitness (log2 ratio)
-7
-6
-5
-4
-3
-2
-1
0
1 at 1009.995 kb on + strand, within MMP_RS05280 at 1010.005 kb on + strand, within MMP_RS05280 at 1010.250 kb on + strand, within MMP_RS05280 at 1010.313 kb on + strand, within MMP_RS05280 at 1010.316 kb on - strand, within MMP_RS05280 at 1010.316 kb on - strand, within MMP_RS05280 at 1010.491 kb on - strand, within MMP_RS05280 at 1010.575 kb on - strand, within MMP_RS05280 at 1010.577 kb on - strand, within MMP_RS05280 at 1010.577 kb on - strand, within MMP_RS05280 at 1010.592 kb on - strand, within MMP_RS05280 at 1010.709 kb on - strand, within MMP_RS05280 at 1010.736 kb on + strand, within MMP_RS05280 at 1010.781 kb on + strand, within MMP_RS05280 at 1010.810 kb on - strand, within MMP_RS05280 at 1010.816 kb on - strand, within MMP_RS05280 at 1011.228 kb on - strand, within MMP_RS05285 at 1011.228 kb on - strand, within MMP_RS05285 at 1011.243 kb on - strand, within MMP_RS05285 at 1011.328 kb on + strand, within MMP_RS05285 at 1011.460 kb on - strand, within MMP_RS05285 at 1011.469 kb on + strand, within MMP_RS05285 at 1011.670 kb on - strand, within MMP_RS05285 at 1011.747 kb on - strand, within MMP_RS05285 at 1011.784 kb on - strand, within MMP_RS05285 at 1011.784 kb on - strand, within MMP_RS05285 at 1011.785 kb on + strand, within MMP_RS05285 at 1011.828 kb on + strand, within MMP_RS05285 at 1011.944 kb on - strand, within MMP_RS05285 at 1012.174 kb on + strand at 1012.197 kb on - strand at 1012.217 kb on - strand at 1012.226 kb on - strand at 1012.280 kb on + strand at 1012.280 kb on + strand at 1012.280 kb on + strand at 1012.280 kb on + strand at 1012.462 kb on - strand, within MMP_RS05290 at 1012.462 kb on - strand, within MMP_RS05290 at 1012.499 kb on - strand, within MMP_RS05290 at 1012.500 kb on + strand, within MMP_RS05290 at 1012.500 kb on + strand, within MMP_RS05290 at 1012.505 kb on + strand, within MMP_RS05290 at 1012.538 kb on + strand, within MMP_RS05290 at 1012.551 kb on + strand, within MMP_RS05290 at 1012.581 kb on + strand, within MMP_RS05290 at 1012.583 kb on - strand, within MMP_RS05290 at 1012.622 kb on - strand, within MMP_RS05290 at 1012.622 kb on - strand, within MMP_RS05290 at 1012.708 kb on - strand, within MMP_RS05290 at 1012.715 kb on + strand, within MMP_RS05290 at 1012.745 kb on + strand, within MMP_RS05290 at 1012.769 kb on - strand, within MMP_RS05290 at 1012.786 kb on + strand at 1012.837 kb on - strand at 1012.837 kb on - strand at 1012.838 kb on + strand at 1012.847 kb on + strand at 1012.851 kb on - strand at 1012.851 kb on - strand at 1012.851 kb on - strand at 1012.865 kb on - strand at 1012.866 kb on + strand at 1012.874 kb on - strand at 1012.874 kb on - strand at 1012.874 kb on - strand at 1012.888 kb on - strand at 1012.892 kb on - strand at 1012.900 kb on - strand at 1012.900 kb on - strand at 1012.940 kb on + strand at 1012.961 kb on - strand at 1012.962 kb on + strand at 1012.966 kb on - strand at 1012.966 kb on - strand at 1012.967 kb on + strand at 1012.971 kb on - strand at 1012.992 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; 1,3-Propanediol_timecourse1; 20 generations remove 1,009,995 + MMP_RS05280 0.36 -3.9 1,010,005 + MMP_RS05280 0.37 -5.8 1,010,250 + MMP_RS05280 0.52 -3.6 1,010,313 + MMP_RS05280 0.56 -3.9 1,010,316 - MMP_RS05280 0.57 -2.8 1,010,316 - MMP_RS05280 0.57 -5.3 1,010,491 - MMP_RS05280 0.68 -4.0 1,010,575 - MMP_RS05280 0.73 -4.8 1,010,577 - MMP_RS05280 0.73 -3.7 1,010,577 - MMP_RS05280 0.73 -7.0 1,010,592 - MMP_RS05280 0.74 -6.5 1,010,709 - MMP_RS05280 0.82 -4.8 1,010,736 + MMP_RS05280 0.83 -3.4 1,010,781 + MMP_RS05280 0.86 -4.8 1,010,810 - MMP_RS05280 0.88 -4.9 1,010,816 - MMP_RS05280 0.88 -4.4 1,011,228 - MMP_RS05285 0.20 -4.7 1,011,228 - MMP_RS05285 0.20 -4.2 1,011,243 - MMP_RS05285 0.22 -4.5 1,011,328 + MMP_RS05285 0.29 -2.0 1,011,460 - MMP_RS05285 0.40 -4.3 1,011,469 + MMP_RS05285 0.41 -4.2 1,011,670 - MMP_RS05285 0.58 -4.7 1,011,747 - MMP_RS05285 0.65 -4.3 1,011,784 - MMP_RS05285 0.68 -4.2 1,011,784 - MMP_RS05285 0.68 -5.9 1,011,785 + MMP_RS05285 0.68 -4.5 1,011,828 + MMP_RS05285 0.72 -3.1 1,011,944 - MMP_RS05285 0.82 -4.5 1,012,174 + -4.5 1,012,197 - -4.0 1,012,217 - -2.9 1,012,226 - -3.4 1,012,280 + -3.7 1,012,280 + -2.7 1,012,280 + -3.7 1,012,280 + -3.8 1,012,462 - MMP_RS05290 0.37 -3.0 1,012,462 - MMP_RS05290 0.37 -4.1 1,012,499 - MMP_RS05290 0.43 -4.6 1,012,500 + MMP_RS05290 0.43 -4.5 1,012,500 + MMP_RS05290 0.43 -5.7 1,012,505 + MMP_RS05290 0.44 -4.2 1,012,538 + MMP_RS05290 0.49 -3.5 1,012,551 + MMP_RS05290 0.51 -4.5 1,012,581 + MMP_RS05290 0.56 -3.7 1,012,583 - MMP_RS05290 0.57 -3.4 1,012,622 - MMP_RS05290 0.63 -3.7 1,012,622 - MMP_RS05290 0.63 -4.1 1,012,708 - MMP_RS05290 0.77 -4.1 1,012,715 + MMP_RS05290 0.79 -2.2 1,012,745 + MMP_RS05290 0.84 -4.6 1,012,769 - MMP_RS05290 0.88 -3.4 1,012,786 + -4.2 1,012,837 - -4.9 1,012,837 - -2.8 1,012,838 + -5.5 1,012,847 + -4.9 1,012,851 - -3.7 1,012,851 - -3.5 1,012,851 - -3.9 1,012,865 - -3.0 1,012,866 + -3.7 1,012,874 - -2.4 1,012,874 - -4.4 1,012,874 - -2.2 1,012,888 - -2.9 1,012,892 - -3.7 1,012,900 - -0.3 1,012,900 - -1.1 1,012,940 + +0.5 1,012,961 - -0.1 1,012,962 + -0.6 1,012,966 - -0.8 1,012,966 - -1.1 1,012,967 + -1.1 1,012,971 - -2.6 1,012,992 + -4.9
Or see this region's nucleotide sequence