Experiment: Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; 1,3-Propanediol_timecourse1; 20 generations
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS03595 and MMP_RS03600 are separated by 78 nucleotides MMP_RS03600 and MMP_RS03605 are separated by 53 nucleotides
MMP_RS03595: MMP_RS03595 - 2-amino-3 7-dideoxy-D-threo-hept-6-ulosonate synthase, at 674,140 to 674,958
_RS03595
MMP_RS03600: MMP_RS03600 - triose-phosphate isomerase, at 675,037 to 675,702
_RS03600
MMP_RS03605: MMP_RS03605 - O-phosphoserine--tRNA ligase, at 675,756 to 677,369
_RS03605
Position (kb) 675
676 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1 at 674.076 kb on + strand at 674.087 kb on - strand at 674.087 kb on - strand at 674.087 kb on - strand at 674.087 kb on - strand at 674.090 kb on + strand at 674.090 kb on + strand at 674.596 kb on - strand at 674.887 kb on + strand at 674.948 kb on - strand at 674.985 kb on - strand at 674.992 kb on + strand at 675.013 kb on + strand at 675.015 kb on - strand at 675.258 kb on - strand, within MMP_RS03600 at 675.259 kb on + strand, within MMP_RS03600 at 675.259 kb on + strand, within MMP_RS03600 at 675.561 kb on + strand, within MMP_RS03600 at 675.579 kb on + strand, within MMP_RS03600 at 675.700 kb on - strand at 675.781 kb on + strand at 676.012 kb on + strand at 676.245 kb on - strand at 676.557 kb on + strand at 676.684 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; 1,3-Propanediol_timecourse1; 20 generations remove 674,076 + -3.4 674,087 - -5.3 674,087 - -4.7 674,087 - -4.0 674,087 - -3.6 674,090 + -3.3 674,090 + -2.5 674,596 - -2.2 674,887 + -2.5 674,948 - -1.0 674,985 - -1.4 674,992 + -3.3 675,013 + -3.3 675,015 - -6.5 675,258 - MMP_RS03600 0.33 -4.6 675,259 + MMP_RS03600 0.33 -4.3 675,259 + MMP_RS03600 0.33 -4.4 675,561 + MMP_RS03600 0.79 -4.1 675,579 + MMP_RS03600 0.81 -2.9 675,700 - +0.2 675,781 + -1.8 676,012 + -1.9 676,245 - -3.0 676,557 + -2.6 676,684 - -2.9
Or see this region's nucleotide sequence