Experiment: Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; 1,3-Propanediol_timecourse1; 20 generations
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS03200 and MMP_RS03205 are separated by 136 nucleotides MMP_RS03205 and MMP_RS03210 are separated by 147 nucleotides MMP_RS03210 and MMP_RS03215 are separated by 52 nucleotides MMP_RS03215 and MMP_RS03220 are separated by 1 nucleotides
MMP_RS03200: MMP_RS03200 - methanogenesis marker 15 protein, at 603,102 to 604,337
_RS03200
MMP_RS03205: MMP_RS03205 - peptidyl-tRNA hydrolase Pth2, at 604,474 to 604,824
_RS03205
MMP_RS03210: MMP_RS03210 - tRNA guanosine(15) transglycosylase TgtA, at 604,972 to 606,921
_RS03210
MMP_RS03215: MMP_RS03215 - MBL fold metallo-hydrolase, at 606,974 to 607,516
_RS03215
MMP_RS03220: MMP_RS03220 - RNA 2' 3'-cyclic phosphodiesterase, at 607,518 to 608,042
_RS03220
Position (kb) 604
605
606
607 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1 at 604.216 kb on - strand at 604.242 kb on + strand at 604.429 kb on - strand at 604.430 kb on + strand at 604.434 kb on - strand at 604.475 kb on - strand at 604.481 kb on + strand at 604.486 kb on - strand at 604.545 kb on - strand, within MMP_RS03205 at 604.699 kb on + strand, within MMP_RS03205 at 604.821 kb on + strand at 604.876 kb on + strand at 604.879 kb on + strand at 604.880 kb on - strand at 604.881 kb on + strand at 604.881 kb on + strand at 604.885 kb on + strand at 604.885 kb on + strand at 604.885 kb on + strand at 604.885 kb on + strand at 604.916 kb on - strand at 604.961 kb on + strand at 604.961 kb on + strand at 605.147 kb on - strand at 605.270 kb on - strand, within MMP_RS03210 at 605.270 kb on - strand, within MMP_RS03210 at 605.271 kb on + strand, within MMP_RS03210 at 605.457 kb on - strand, within MMP_RS03210 at 605.467 kb on + strand, within MMP_RS03210 at 605.483 kb on + strand, within MMP_RS03210 at 605.555 kb on - strand, within MMP_RS03210 at 605.555 kb on - strand, within MMP_RS03210 at 605.601 kb on - strand, within MMP_RS03210 at 605.749 kb on - strand, within MMP_RS03210 at 605.822 kb on - strand, within MMP_RS03210 at 605.823 kb on + strand, within MMP_RS03210 at 605.823 kb on + strand, within MMP_RS03210 at 605.823 kb on + strand, within MMP_RS03210 at 605.833 kb on - strand, within MMP_RS03210 at 605.907 kb on - strand, within MMP_RS03210 at 606.018 kb on - strand, within MMP_RS03210 at 606.028 kb on - strand, within MMP_RS03210 at 606.092 kb on + strand, within MMP_RS03210 at 606.106 kb on + strand, within MMP_RS03210 at 606.106 kb on + strand, within MMP_RS03210 at 606.163 kb on - strand, within MMP_RS03210 at 606.187 kb on + strand, within MMP_RS03210 at 606.212 kb on - strand, within MMP_RS03210 at 606.213 kb on + strand, within MMP_RS03210 at 606.240 kb on + strand, within MMP_RS03210 at 606.401 kb on - strand, within MMP_RS03210 at 606.410 kb on + strand, within MMP_RS03210 at 606.410 kb on + strand, within MMP_RS03210 at 606.415 kb on - strand, within MMP_RS03210 at 606.419 kb on + strand, within MMP_RS03210 at 606.422 kb on - strand, within MMP_RS03210 at 606.423 kb on + strand, within MMP_RS03210 at 606.423 kb on + strand, within MMP_RS03210 at 606.503 kb on + strand, within MMP_RS03210 at 606.665 kb on - strand, within MMP_RS03210 at 606.698 kb on - strand, within MMP_RS03210 at 606.848 kb on - strand at 606.851 kb on - strand at 606.851 kb on - strand at 606.934 kb on + strand at 606.944 kb on - strand at 606.990 kb on + strand at 607.054 kb on - strand, within MMP_RS03215 at 607.055 kb on + strand, within MMP_RS03215 at 607.059 kb on + strand, within MMP_RS03215 at 607.170 kb on + strand, within MMP_RS03215 at 607.172 kb on + strand, within MMP_RS03215 at 607.172 kb on + strand, within MMP_RS03215 at 607.174 kb on - strand, within MMP_RS03215 at 607.175 kb on + strand, within MMP_RS03215 at 607.177 kb on + strand, within MMP_RS03215 at 607.177 kb on + strand, within MMP_RS03215 at 607.177 kb on + strand, within MMP_RS03215 at 607.177 kb on + strand, within MMP_RS03215 at 607.241 kb on + strand, within MMP_RS03215 at 607.330 kb on + strand, within MMP_RS03215 at 607.343 kb on - strand, within MMP_RS03215 at 607.405 kb on - strand, within MMP_RS03215 at 607.414 kb on + strand, within MMP_RS03215 at 607.414 kb on + strand, within MMP_RS03215 at 607.453 kb on + strand, within MMP_RS03215 at 607.474 kb on + strand at 607.474 kb on + strand at 607.520 kb on + strand at 607.559 kb on - strand at 607.560 kb on + strand at 607.560 kb on + strand at 607.569 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; 1,3-Propanediol_timecourse1; 20 generations remove 604,216 - -3.5 604,242 + -4.7 604,429 - -0.6 604,430 + +0.2 604,434 - -0.7 604,475 - +0.3 604,481 + -5.7 604,486 - -6.6 604,545 - MMP_RS03205 0.20 -5.9 604,699 + MMP_RS03205 0.64 -4.4 604,821 + -5.4 604,876 + -3.6 604,879 + -6.3 604,880 - -3.7 604,881 + -5.8 604,881 + -3.0 604,885 + -3.9 604,885 + -2.0 604,885 + -2.8 604,885 + -3.2 604,916 - -0.6 604,961 + -0.6 604,961 + -0.6 605,147 - -1.0 605,270 - MMP_RS03210 0.15 -1.0 605,270 - MMP_RS03210 0.15 -2.2 605,271 + MMP_RS03210 0.15 -1.3 605,457 - MMP_RS03210 0.25 -1.5 605,467 + MMP_RS03210 0.25 -2.3 605,483 + MMP_RS03210 0.26 -1.9 605,555 - MMP_RS03210 0.30 -1.1 605,555 - MMP_RS03210 0.30 -1.2 605,601 - MMP_RS03210 0.32 -1.5 605,749 - MMP_RS03210 0.40 -1.1 605,822 - MMP_RS03210 0.44 -2.6 605,823 + MMP_RS03210 0.44 -0.7 605,823 + MMP_RS03210 0.44 -1.5 605,823 + MMP_RS03210 0.44 -1.6 605,833 - MMP_RS03210 0.44 -0.6 605,907 - MMP_RS03210 0.48 -1.5 606,018 - MMP_RS03210 0.54 -0.6 606,028 - MMP_RS03210 0.54 -0.4 606,092 + MMP_RS03210 0.57 -1.0 606,106 + MMP_RS03210 0.58 -1.4 606,106 + MMP_RS03210 0.58 -1.5 606,163 - MMP_RS03210 0.61 -1.6 606,187 + MMP_RS03210 0.62 -0.9 606,212 - MMP_RS03210 0.64 -0.8 606,213 + MMP_RS03210 0.64 -1.1 606,240 + MMP_RS03210 0.65 -0.9 606,401 - MMP_RS03210 0.73 -1.7 606,410 + MMP_RS03210 0.74 -1.6 606,410 + MMP_RS03210 0.74 -2.0 606,415 - MMP_RS03210 0.74 -1.3 606,419 + MMP_RS03210 0.74 -1.5 606,422 - MMP_RS03210 0.74 -1.5 606,423 + MMP_RS03210 0.74 -1.4 606,423 + MMP_RS03210 0.74 -1.4 606,503 + MMP_RS03210 0.79 -1.2 606,665 - MMP_RS03210 0.87 -1.4 606,698 - MMP_RS03210 0.89 -1.5 606,848 - -0.7 606,851 - -2.0 606,851 - -0.5 606,934 + -0.4 606,944 - +0.1 606,990 + +0.7 607,054 - MMP_RS03215 0.15 +0.4 607,055 + MMP_RS03215 0.15 +0.8 607,059 + MMP_RS03215 0.16 -0.2 607,170 + MMP_RS03215 0.36 -0.2 607,172 + MMP_RS03215 0.36 -0.6 607,172 + MMP_RS03215 0.36 +0.1 607,174 - MMP_RS03215 0.37 +1.0 607,175 + MMP_RS03215 0.37 -0.1 607,177 + MMP_RS03215 0.37 +1.1 607,177 + MMP_RS03215 0.37 +0.3 607,177 + MMP_RS03215 0.37 -0.4 607,177 + MMP_RS03215 0.37 -0.5 607,241 + MMP_RS03215 0.49 -0.0 607,330 + MMP_RS03215 0.66 +0.3 607,343 - MMP_RS03215 0.68 +0.4 607,405 - MMP_RS03215 0.79 -0.4 607,414 + MMP_RS03215 0.81 +1.0 607,414 + MMP_RS03215 0.81 +0.2 607,453 + MMP_RS03215 0.88 -0.0 607,474 + -0.1 607,474 + -0.0 607,520 + +0.6 607,559 - -0.3 607,560 + +0.5 607,560 + -0.8 607,569 + +0.9
Or see this region's nucleotide sequence