Experiment: Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; 1,3-Propanediol_timecourse1; 20 generations
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS02155 and MMP_RS02160 are separated by 105 nucleotides MMP_RS02160 and MMP_RS02165 are separated by 27 nucleotides MMP_RS02165 and MMP_RS02170 are separated by 14 nucleotides MMP_RS02170 and MMP_RS02175 are separated by 158 nucleotides
MMP_RS02155: MMP_RS02155 - 2-phospho-L-lactate transferase, at 403,481 to 404,410
_RS02155
MMP_RS02160: MMP_RS02160 - GTP cyclohydrolase IIa, at 404,516 to 405,316
_RS02160
MMP_RS02165: MMP_RS02165 - tRNA-dihydrouridine synthase, at 405,344 to 406,114
_RS02165
MMP_RS02170: MMP_RS02170 - CBS domain-containing protein, at 406,129 to 406,542
_RS02170
MMP_RS02175: MMP_RS02175 - DUF1611 domain-containing protein, at 406,701 to 407,621
_RS02175
Position (kb) 405
406
407 Strain fitness (log2 ratio) -8
-7
-6
-5
-4
-3
-2
-1
0
1 at 404.452 kb on - strand at 404.455 kb on + strand at 404.500 kb on + strand at 404.511 kb on - strand at 404.619 kb on + strand at 405.112 kb on - strand at 405.289 kb on + strand at 405.294 kb on + strand at 405.299 kb on - strand at 405.299 kb on - strand at 405.354 kb on - strand at 405.434 kb on - strand, within MMP_RS02165 at 405.657 kb on - strand, within MMP_RS02165 at 405.658 kb on + strand, within MMP_RS02165 at 405.682 kb on - strand, within MMP_RS02165 at 405.842 kb on + strand, within MMP_RS02165 at 405.910 kb on - strand, within MMP_RS02165 at 405.910 kb on - strand, within MMP_RS02165 at 405.910 kb on - strand, within MMP_RS02165 at 405.911 kb on + strand, within MMP_RS02165 at 405.913 kb on + strand, within MMP_RS02165 at 405.913 kb on + strand, within MMP_RS02165 at 405.913 kb on + strand, within MMP_RS02165 at 405.913 kb on + strand, within MMP_RS02165 at 405.913 kb on + strand, within MMP_RS02165 at 405.913 kb on + strand, within MMP_RS02165 at 405.949 kb on + strand, within MMP_RS02165 at 406.078 kb on + strand at 406.087 kb on - strand at 406.087 kb on - strand at 406.088 kb on + strand at 406.117 kb on + strand at 406.117 kb on + strand at 406.117 kb on + strand at 406.121 kb on - strand at 406.130 kb on - strand at 406.135 kb on - strand at 406.200 kb on - strand, within MMP_RS02170 at 406.220 kb on + strand, within MMP_RS02170 at 406.354 kb on - strand, within MMP_RS02170 at 406.354 kb on - strand, within MMP_RS02170 at 406.509 kb on - strand at 406.562 kb on - strand at 406.566 kb on + strand at 406.592 kb on + strand at 406.602 kb on + strand at 406.626 kb on - strand at 406.770 kb on + strand at 406.772 kb on + strand at 406.772 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; 1,3-Propanediol_timecourse1; 20 generations remove 404,452 - -4.9 404,455 + -4.8 404,500 + -4.1 404,511 - -5.3 404,619 + -5.5 405,112 - -3.9 405,289 + -4.0 405,294 + -4.5 405,299 - -5.2 405,299 - -5.0 405,354 - -6.4 405,434 - MMP_RS02165 0.12 -4.5 405,657 - MMP_RS02165 0.41 -5.2 405,658 + MMP_RS02165 0.41 -6.3 405,682 - MMP_RS02165 0.44 -4.3 405,842 + MMP_RS02165 0.65 -3.7 405,910 - MMP_RS02165 0.73 -8.3 405,910 - MMP_RS02165 0.73 -6.0 405,910 - MMP_RS02165 0.73 -6.6 405,911 + MMP_RS02165 0.74 -5.1 405,913 + MMP_RS02165 0.74 -3.3 405,913 + MMP_RS02165 0.74 -5.4 405,913 + MMP_RS02165 0.74 -4.9 405,913 + MMP_RS02165 0.74 -5.6 405,913 + MMP_RS02165 0.74 -5.5 405,913 + MMP_RS02165 0.74 -6.7 405,949 + MMP_RS02165 0.78 -4.3 406,078 + -6.8 406,087 - -3.1 406,087 - -5.1 406,088 + -4.3 406,117 + +0.4 406,117 + -0.2 406,117 + +0.4 406,121 - +0.1 406,130 - +0.3 406,135 - +0.0 406,200 - MMP_RS02170 0.17 -0.3 406,220 + MMP_RS02170 0.22 +0.3 406,354 - MMP_RS02170 0.54 -0.6 406,354 - MMP_RS02170 0.54 -0.3 406,509 - -0.1 406,562 - -0.1 406,566 + -0.3 406,592 + -1.7 406,602 + -0.2 406,626 - -1.5 406,770 + -1.8 406,772 + -2.1 406,772 + -1.0
Or see this region's nucleotide sequence