Experiment: Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; 1,3-Propanediol_timecourse1; 20 generations
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS01975 and MMP_RS01980 are separated by 133 nucleotides MMP_RS01980 and MMP_RS01985 are separated by 79 nucleotides MMP_RS01985 and MMP_RS01990 are separated by 203 nucleotides MMP_RS01990 and MMP_RS01995 are separated by 39 nucleotides
MMP_RS01975: MMP_RS01975 - hypothetical protein, at 366,983 to 368,389
_RS01975
MMP_RS01980: MMP_RS01980 - trimeric intracellular cation channel family protein, at 368,523 to 369,152
_RS01980
MMP_RS01985: MMP_RS01985 - F420-dependent methylenetetrahydromethanopterin dehydrogenase, at 369,232 to 370,065
_RS01985
MMP_RS01990: MMP_RS01990 - hypothetical protein, at 370,269 to 370,517
_RS01990
MMP_RS01995: MMP_RS01995 - ribonuclease VapC5, at 370,557 to 371,087
_RS01995
Position (kb)
369
370
371 Strain fitness (log2 ratio)
-8
-7
-6
-5
-4
-3
-2
-1
0
1 at 368.273 kb on - strand at 368.320 kb on + strand at 368.327 kb on + strand at 368.331 kb on - strand at 368.331 kb on - strand at 368.354 kb on + strand at 368.365 kb on + strand at 368.374 kb on + strand at 368.380 kb on - strand at 368.399 kb on - strand at 368.448 kb on - strand at 368.470 kb on + strand at 368.471 kb on - strand at 368.473 kb on - strand at 368.474 kb on + strand at 368.493 kb on - strand at 368.494 kb on + strand at 368.494 kb on + strand at 368.497 kb on - strand at 368.497 kb on - strand at 368.528 kb on - strand at 368.604 kb on + strand, within MMP_RS01980 at 368.615 kb on + strand, within MMP_RS01980 at 368.646 kb on + strand, within MMP_RS01980 at 368.691 kb on + strand, within MMP_RS01980 at 368.707 kb on - strand, within MMP_RS01980 at 368.788 kb on - strand, within MMP_RS01980 at 368.788 kb on - strand, within MMP_RS01980 at 368.788 kb on - strand, within MMP_RS01980 at 368.789 kb on + strand, within MMP_RS01980 at 368.789 kb on + strand, within MMP_RS01980 at 368.799 kb on - strand, within MMP_RS01980 at 368.799 kb on - strand, within MMP_RS01980 at 368.807 kb on + strand, within MMP_RS01980 at 368.865 kb on - strand, within MMP_RS01980 at 368.866 kb on + strand, within MMP_RS01980 at 368.867 kb on - strand, within MMP_RS01980 at 368.868 kb on + strand, within MMP_RS01980 at 368.894 kb on + strand, within MMP_RS01980 at 368.898 kb on + strand, within MMP_RS01980 at 368.935 kb on - strand, within MMP_RS01980 at 368.936 kb on + strand, within MMP_RS01980 at 368.936 kb on + strand, within MMP_RS01980 at 368.936 kb on + strand, within MMP_RS01980 at 368.944 kb on - strand, within MMP_RS01980 at 368.950 kb on - strand, within MMP_RS01980 at 368.986 kb on + strand, within MMP_RS01980 at 369.022 kb on + strand, within MMP_RS01980 at 369.157 kb on - strand at 369.212 kb on - strand at 369.256 kb on + strand at 369.361 kb on - strand, within MMP_RS01985 at 369.647 kb on + strand, within MMP_RS01985 at 369.647 kb on + strand, within MMP_RS01985 at 369.683 kb on + strand, within MMP_RS01985 at 369.890 kb on + strand, within MMP_RS01985 at 369.890 kb on + strand, within MMP_RS01985 at 370.067 kb on + strand at 370.067 kb on + strand at 370.067 kb on + strand at 370.100 kb on - strand at 370.120 kb on - strand at 370.121 kb on + strand at 370.178 kb on - strand at 370.202 kb on + strand at 370.202 kb on + strand at 370.215 kb on - strand at 370.223 kb on - strand at 370.318 kb on - strand, within MMP_RS01990 at 370.318 kb on - strand, within MMP_RS01990 at 370.344 kb on + strand, within MMP_RS01990 at 370.382 kb on + strand, within MMP_RS01990 at 370.516 kb on + strand at 370.554 kb on - strand at 370.555 kb on + strand at 370.555 kb on + strand at 370.591 kb on - strand at 370.592 kb on + strand at 370.811 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; 1,3-Propanediol_timecourse1; 20 generations remove 368,273 - -4.5 368,320 + -1.8 368,327 + -5.1 368,331 - -5.8 368,331 - -4.6 368,354 + -3.6 368,365 + +0.2 368,374 + -0.2 368,380 - +0.2 368,399 - +0.6 368,448 - +0.5 368,470 + +0.4 368,471 - +0.1 368,473 - +0.6 368,474 + +0.4 368,493 - +0.8 368,494 + -0.1 368,494 + -0.2 368,497 - +0.2 368,497 - +0.7 368,528 - +0.4 368,604 + MMP_RS01980 0.13 +0.0 368,615 + MMP_RS01980 0.15 +0.8 368,646 + MMP_RS01980 0.20 +0.2 368,691 + MMP_RS01980 0.27 -0.1 368,707 - MMP_RS01980 0.29 +0.2 368,788 - MMP_RS01980 0.42 +0.5 368,788 - MMP_RS01980 0.42 +0.7 368,788 - MMP_RS01980 0.42 +0.9 368,789 + MMP_RS01980 0.42 +0.3 368,789 + MMP_RS01980 0.42 +0.5 368,799 - MMP_RS01980 0.44 -0.1 368,799 - MMP_RS01980 0.44 +0.2 368,807 + MMP_RS01980 0.45 +0.5 368,865 - MMP_RS01980 0.54 +0.2 368,866 + MMP_RS01980 0.54 +0.7 368,867 - MMP_RS01980 0.55 +0.5 368,868 + MMP_RS01980 0.55 +0.9 368,894 + MMP_RS01980 0.59 +0.3 368,898 + MMP_RS01980 0.60 +0.1 368,935 - MMP_RS01980 0.65 +1.1 368,936 + MMP_RS01980 0.66 +0.3 368,936 + MMP_RS01980 0.66 +1.0 368,936 + MMP_RS01980 0.66 +0.5 368,944 - MMP_RS01980 0.67 +0.6 368,950 - MMP_RS01980 0.68 +0.1 368,986 + MMP_RS01980 0.73 +0.9 369,022 + MMP_RS01980 0.79 +0.7 369,157 - +0.3 369,212 - -4.7 369,256 + -6.7 369,361 - MMP_RS01985 0.15 -7.7 369,647 + MMP_RS01985 0.50 -7.4 369,647 + MMP_RS01985 0.50 -5.5 369,683 + MMP_RS01985 0.54 -6.8 369,890 + MMP_RS01985 0.79 -6.3 369,890 + MMP_RS01985 0.79 -8.3 370,067 + -7.2 370,067 + -6.3 370,067 + -6.0 370,100 - -5.8 370,120 - -6.1 370,121 + -6.2 370,178 - +0.7 370,202 + +0.3 370,202 + +0.2 370,215 - -0.0 370,223 - -0.1 370,318 - MMP_RS01990 0.20 -0.3 370,318 - MMP_RS01990 0.20 +0.3 370,344 + MMP_RS01990 0.30 +0.1 370,382 + MMP_RS01990 0.45 -0.0 370,516 + +0.9 370,554 - +0.3 370,555 + +0.6 370,555 + +0.6 370,591 - +0.2 370,592 + +0.3 370,811 - -4.4
Or see this region's nucleotide sequence