Experiment: Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; 1,3-Propanediol_timecourse1; 20 generations
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS01785 and MMP_RS01790 are separated by 47 nucleotides MMP_RS01790 and MMP_RS01795 are separated by 16 nucleotides MMP_RS01795 and MMP_RS01800 are separated by 28 nucleotides MMP_RS01800 and MMP_RS01805 are separated by 118 nucleotides MMP_RS01805 and MMP_RS01810 are separated by 110 nucleotides MMP_RS01810 and MMP_RS01815 are separated by 27 nucleotides
MMP_RS01785: MMP_RS01785 - hypothetical protein, at 332,330 to 332,743
_RS01785
MMP_RS01790: MMP_RS01790 - restriction endonuclease, at 332,791 to 333,198
_RS01790
MMP_RS01795: MMP_RS01795 - methanogenesis marker 6 protein, at 333,215 to 333,661
_RS01795
MMP_RS01800: MMP_RS01800 - TIGR00303 family protein, at 333,690 to 334,742
_RS01800
MMP_RS01805: MMP_RS01805 - NUDIX hydrolase, at 334,861 to 335,376
_RS01805
MMP_RS01810: MMP_RS01810 - tRNA-Arg, at 335,487 to 335,564
_RS01810
MMP_RS01815: MMP_RS01815 - tRNA-Glu, at 335,592 to 335,666
_RS01815
Position (kb) 333
334
335 Strain fitness (log2 ratio) -7
-6
-5
-4
-3
-2
-1
0
1
2 at 332.696 kb on - strand, within MMP_RS01785 at 332.696 kb on - strand, within MMP_RS01785 at 332.697 kb on + strand, within MMP_RS01785 at 332.697 kb on + strand, within MMP_RS01785 at 332.700 kb on - strand, within MMP_RS01785 at 332.700 kb on - strand, within MMP_RS01785 at 332.701 kb on + strand, within MMP_RS01785 at 332.727 kb on - strand at 332.731 kb on - strand at 332.779 kb on + strand at 332.779 kb on + strand at 332.788 kb on + strand at 332.789 kb on - strand at 332.789 kb on - strand at 332.790 kb on + strand at 332.790 kb on + strand at 332.792 kb on - strand at 333.021 kb on + strand, within MMP_RS01790 at 333.023 kb on - strand, within MMP_RS01790 at 333.023 kb on - strand, within MMP_RS01790 at 333.024 kb on + strand, within MMP_RS01790 at 333.045 kb on - strand, within MMP_RS01790 at 333.089 kb on + strand, within MMP_RS01790 at 333.154 kb on - strand, within MMP_RS01790 at 333.189 kb on - strand at 333.213 kb on + strand at 333.461 kb on + strand, within MMP_RS01795 at 333.467 kb on + strand, within MMP_RS01795 at 333.500 kb on - strand, within MMP_RS01795 at 333.672 kb on - strand at 333.736 kb on - strand at 333.829 kb on - strand, within MMP_RS01800 at 334.047 kb on - strand, within MMP_RS01800 at 334.048 kb on + strand, within MMP_RS01800 at 334.059 kb on + strand, within MMP_RS01800 at 334.222 kb on + strand, within MMP_RS01800 at 334.537 kb on + strand, within MMP_RS01800 at 334.569 kb on - strand, within MMP_RS01800 at 334.572 kb on + strand, within MMP_RS01800 at 334.576 kb on + strand, within MMP_RS01800 at 334.603 kb on - strand, within MMP_RS01800 at 334.666 kb on + strand at 334.668 kb on + strand at 334.680 kb on - strand at 334.734 kb on + strand at 334.771 kb on + strand at 334.774 kb on - strand at 334.778 kb on + strand at 334.870 kb on + strand at 334.874 kb on - strand at 335.014 kb on - strand, within MMP_RS01805 at 335.056 kb on - strand, within MMP_RS01805 at 335.292 kb on - strand, within MMP_RS01805 at 335.293 kb on + strand, within MMP_RS01805 at 335.306 kb on - strand, within MMP_RS01805 at 335.362 kb on - strand at 335.363 kb on + strand at 335.364 kb on - strand at 335.364 kb on - strand at 335.364 kb on - strand at 335.366 kb on - strand at 335.398 kb on + strand at 335.458 kb on - strand at 335.721 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; 1,3-Propanediol_timecourse1; 20 generations remove 332,696 - MMP_RS01785 0.88 -1.3 332,696 - MMP_RS01785 0.88 +1.1 332,697 + MMP_RS01785 0.89 +1.3 332,697 + MMP_RS01785 0.89 +1.3 332,700 - MMP_RS01785 0.89 +0.8 332,700 - MMP_RS01785 0.89 +1.0 332,701 + MMP_RS01785 0.90 +0.5 332,727 - +0.9 332,731 - +1.1 332,779 + +0.3 332,779 + +0.8 332,788 + +0.5 332,789 - +0.2 332,789 - -0.1 332,790 + +0.6 332,790 + +0.4 332,792 - -0.4 333,021 + MMP_RS01790 0.56 +0.3 333,023 - MMP_RS01790 0.57 -0.2 333,023 - MMP_RS01790 0.57 +0.0 333,024 + MMP_RS01790 0.57 +0.1 333,045 - MMP_RS01790 0.62 +0.0 333,089 + MMP_RS01790 0.73 +0.3 333,154 - MMP_RS01790 0.89 +0.0 333,189 - -0.1 333,213 + -0.8 333,461 + MMP_RS01795 0.55 -4.8 333,467 + MMP_RS01795 0.56 -5.5 333,500 - MMP_RS01795 0.64 -4.3 333,672 - -3.8 333,736 - -3.2 333,829 - MMP_RS01800 0.13 -4.5 334,047 - MMP_RS01800 0.34 -4.3 334,048 + MMP_RS01800 0.34 -5.2 334,059 + MMP_RS01800 0.35 -4.6 334,222 + MMP_RS01800 0.51 -2.9 334,537 + MMP_RS01800 0.80 -4.9 334,569 - MMP_RS01800 0.83 -4.7 334,572 + MMP_RS01800 0.84 -5.2 334,576 + MMP_RS01800 0.84 -5.2 334,603 - MMP_RS01800 0.87 -4.1 334,666 + -5.1 334,668 + -4.1 334,680 - -3.4 334,734 + -5.2 334,771 + -6.8 334,774 - -0.1 334,778 + -6.1 334,870 + +0.4 334,874 - -0.5 335,014 - MMP_RS01805 0.30 +0.5 335,056 - MMP_RS01805 0.38 +0.5 335,292 - MMP_RS01805 0.84 -0.8 335,293 + MMP_RS01805 0.84 -0.0 335,306 - MMP_RS01805 0.86 -0.2 335,362 - -0.1 335,363 + +0.2 335,364 - +2.6 335,364 - +0.3 335,364 - +0.5 335,366 - -0.2 335,398 + +0.9 335,458 - -3.9 335,721 - +0.3
Or see this region's nucleotide sequence