Experiment: Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; 1,3-Propanediol_timecourse1; 20 generations
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS00680 and MMP_RS00685 are separated by 23 nucleotides MMP_RS00685 and MMP_RS00690 are separated by 46 nucleotides MMP_RS00690 and MMP_RS00695 are separated by 12 nucleotides
MMP_RS00680: MMP_RS00680 - N-acetyl-gamma-glutamyl-phosphate reductase, at 124,154 to 125,185
_RS00680
MMP_RS00685: MMP_RS00685 - 2-phospho-L-lactate guanylyltransferase, at 125,209 to 125,877
_RS00685
MMP_RS00690: MMP_RS00690 - MBL fold metallo-hydrolase, at 125,924 to 126,541
_RS00690
MMP_RS00695: MMP_RS00695 - biotin--[acetyl-CoA-carboxylase] ligase, at 126,554 to 127,249
_RS00695
Position (kb) 125
126 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1 at 124.339 kb on - strand at 124.480 kb on - strand at 124.484 kb on - strand at 124.485 kb on + strand at 124.873 kb on + strand at 124.907 kb on + strand at 124.907 kb on + strand at 124.908 kb on - strand at 124.908 kb on - strand at 124.908 kb on - strand at 124.909 kb on + strand at 124.946 kb on - strand at 125.264 kb on + strand at 125.289 kb on + strand, within MMP_RS00685 at 125.289 kb on + strand, within MMP_RS00685 at 125.344 kb on - strand, within MMP_RS00685 at 125.344 kb on - strand, within MMP_RS00685 at 125.351 kb on + strand, within MMP_RS00685 at 125.351 kb on + strand, within MMP_RS00685 at 125.358 kb on - strand, within MMP_RS00685 at 125.785 kb on - strand, within MMP_RS00685 at 125.906 kb on - strand at 125.925 kb on - strand at 125.930 kb on + strand at 125.958 kb on + strand at 125.988 kb on + strand, within MMP_RS00690 at 126.008 kb on + strand, within MMP_RS00690 at 126.009 kb on - strand, within MMP_RS00690 at 126.131 kb on - strand, within MMP_RS00690 at 126.170 kb on - strand, within MMP_RS00690 at 126.263 kb on + strand, within MMP_RS00690 at 126.271 kb on - strand, within MMP_RS00690 at 126.280 kb on - strand, within MMP_RS00690 at 126.305 kb on - strand, within MMP_RS00690 at 126.305 kb on - strand, within MMP_RS00690 at 126.305 kb on - strand, within MMP_RS00690 at 126.318 kb on + strand, within MMP_RS00690 at 126.391 kb on - strand, within MMP_RS00690 at 126.391 kb on - strand, within MMP_RS00690 at 126.396 kb on - strand, within MMP_RS00690 at 126.491 kb on - strand at 126.671 kb on - strand, within MMP_RS00695 at 126.821 kb on - strand, within MMP_RS00695
Per-strain Table
Position Strand Gene LocusTag Fraction Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; 1,3-Propanediol_timecourse1; 20 generations remove 124,339 - -2.8 124,480 - -3.9 124,484 - -3.8 124,485 + -4.2 124,873 + -4.4 124,907 + -2.6 124,907 + -4.2 124,908 - -4.6 124,908 - -3.8 124,908 - -5.6 124,909 + -4.6 124,946 - -3.3 125,264 + -4.6 125,289 + MMP_RS00685 0.12 -4.6 125,289 + MMP_RS00685 0.12 -6.1 125,344 - MMP_RS00685 0.20 -3.4 125,344 - MMP_RS00685 0.20 -5.7 125,351 + MMP_RS00685 0.21 -4.3 125,351 + MMP_RS00685 0.21 -5.9 125,358 - MMP_RS00685 0.22 -5.3 125,785 - MMP_RS00685 0.86 -5.4 125,906 - -3.5 125,925 - -2.9 125,930 + -0.1 125,958 + +1.0 125,988 + MMP_RS00690 0.10 -0.5 126,008 + MMP_RS00690 0.14 -0.2 126,009 - MMP_RS00690 0.14 +0.4 126,131 - MMP_RS00690 0.33 -0.0 126,170 - MMP_RS00690 0.40 +0.4 126,263 + MMP_RS00690 0.55 +0.0 126,271 - MMP_RS00690 0.56 +0.4 126,280 - MMP_RS00690 0.58 +0.8 126,305 - MMP_RS00690 0.62 +0.6 126,305 - MMP_RS00690 0.62 -0.0 126,305 - MMP_RS00690 0.62 +0.1 126,318 + MMP_RS00690 0.64 +0.8 126,391 - MMP_RS00690 0.76 +1.1 126,391 - MMP_RS00690 0.76 +0.0 126,396 - MMP_RS00690 0.76 +0.5 126,491 - +0.2 126,671 - MMP_RS00695 0.17 -4.6 126,821 - MMP_RS00695 0.38 -4.6
Or see this region's nucleotide sequence