Strain Fitness in Methanococcus maripaludis S2 around MMP_RS00515

Experiment: Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; 1,3-Propanediol_timecourse1; 20 generations

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntMMP_RS00510 and MMP_RS00515 are separated by 20 nucleotidesMMP_RS00515 and MMP_RS00520 are separated by 100 nucleotidesMMP_RS00520 and MMP_RS00525 are separated by 48 nucleotidesMMP_RS00525 and MMP_RS00530 are separated by 85 nucleotides MMP_RS00510: MMP_RS00510 - glycosyltransferase family 4 protein, at 100,750 to 101,817 _RS00510 MMP_RS00515: MMP_RS00515 - DUF655 domain-containing protein, at 101,838 to 102,422 _RS00515 MMP_RS00520: MMP_RS00520 - RNA polymerase Rpb4 family protein, at 102,523 to 102,846 _RS00520 MMP_RS00525: MMP_RS00525 - 50S ribosomal protein L21e, at 102,895 to 103,188 _RS00525 MMP_RS00530: MMP_RS00530 - tRNA pseudouridine(54/55) synthase Pus10, at 103,274 to 104,686 _RS00530 Position (kb) 101 102 103Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1at 101.209 kb on + strandat 101.318 kb on - strandat 101.319 kb on + strandat 101.612 kb on - strandat 101.729 kb on + strandat 101.793 kb on + strandat 101.793 kb on + strandat 101.795 kb on + strandat 102.041 kb on - strand, within MMP_RS00515at 102.335 kb on - strand, within MMP_RS00515at 102.373 kb on - strandat 102.379 kb on - strandat 102.454 kb on + strandat 102.460 kb on + strandat 102.463 kb on - strandat 102.463 kb on - strandat 102.479 kb on - strandat 102.541 kb on - strandat 102.622 kb on - strand, within MMP_RS00520at 102.706 kb on - strand, within MMP_RS00520at 102.758 kb on + strand, within MMP_RS00520at 103.258 kb on - strandat 103.296 kb on - strandat 103.393 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; 1,3-Propanediol_timecourse1; 20 generations
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101,209 + -2.1
101,318 - -5.3
101,319 + -4.6
101,612 - -4.9
101,729 + -4.4
101,793 + -5.7
101,793 + -4.5
101,795 + -5.4
102,041 - MMP_RS00515 0.35 -4.8
102,335 - MMP_RS00515 0.85 -4.8
102,373 - -5.0
102,379 - -5.1
102,454 + -4.7
102,460 + -4.9
102,463 - -4.1
102,463 - -4.9
102,479 - -3.4
102,541 - +0.5
102,622 - MMP_RS00520 0.31 -0.9
102,706 - MMP_RS00520 0.56 -2.7
102,758 + MMP_RS00520 0.73 -1.9
103,258 - -2.4
103,296 - -0.2
103,393 - -0.7

Or see this region's nucleotide sequence