Experiment: Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; 1,3-Propanediol_timecourse1; 20 generations
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS00495 and MMP_RS00500 overlap by 11 nucleotides MMP_RS00500 and MMP_RS00505 are separated by 4 nucleotides MMP_RS00505 and MMP_RS00510 are separated by 14 nucleotides
MMP_RS00495: MMP_RS00495 - hydroxymethylglutaryl-CoA reductase (NADPH), at 97,761 to 98,987
_RS00495
MMP_RS00500: MMP_RS00500 - glutamyl-tRNA reductase, at 98,977 to 100,125
_RS00500
MMP_RS00505: MMP_RS00505 - bifunctional precorrin-2 dehydrogenase/sirohydrochlorin ferrochelatase, at 100,130 to 100,735
_RS00505
MMP_RS00510: MMP_RS00510 - glycosyltransferase family 4 protein, at 100,750 to 101,817
_RS00510
Position (kb) 98
99
100
101 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 98.085 kb on + strand at 98.470 kb on + strand at 98.639 kb on - strand at 98.643 kb on + strand at 98.781 kb on + strand at 100.089 kb on - strand at 100.771 kb on + strand at 100.774 kb on - strand at 100.781 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; 1,3-Propanediol_timecourse1; 20 generations remove 98,085 + -4.7 98,470 + -4.5 98,639 - -5.2 98,643 + -4.2 98,781 + -5.1 100,089 - -5.3 100,771 + -5.6 100,774 - -1.0 100,781 - -4.5
Or see this region's nucleotide sequence