Experiment: Ammonium chloride (N); RCH2 media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS21150 and EX31_RS21155 overlap by 8 nucleotides EX31_RS21155 and EX31_RS21160 overlap by 1 nucleotides EX31_RS21160 and EX31_RS21165 are separated by 177 nucleotides
EX31_RS21150: EX31_RS21150 - allantoinase PuuE, at 873,696 to 874,634
_RS21150
EX31_RS21155: EX31_RS21155 - Asp/Glu racemase, at 874,627 to 875,385
_RS21155
EX31_RS21160: EX31_RS21160 - GntR family transcriptional regulator, at 875,385 to 876,119
_RS21160
EX31_RS21165: EX31_RS21165 - NCS1 family nucleobase:cation symporter-1, at 876,297 to 877,799
_RS21165
Position (kb) 874
875
876 Strain fitness (log2 ratio) -2
-1
0
1
2 at 873.744 kb on - strand at 873.775 kb on + strand at 873.776 kb on - strand at 873.877 kb on + strand, within EX31_RS21150 at 873.878 kb on - strand, within EX31_RS21150 at 873.878 kb on - strand, within EX31_RS21150 at 873.878 kb on - strand, within EX31_RS21150 at 873.904 kb on + strand, within EX31_RS21150 at 873.904 kb on + strand, within EX31_RS21150 at 873.905 kb on - strand, within EX31_RS21150 at 873.908 kb on + strand, within EX31_RS21150 at 873.908 kb on + strand, within EX31_RS21150 at 873.908 kb on + strand, within EX31_RS21150 at 873.908 kb on + strand, within EX31_RS21150 at 873.908 kb on + strand, within EX31_RS21150 at 873.908 kb on + strand, within EX31_RS21150 at 873.908 kb on + strand, within EX31_RS21150 at 873.909 kb on - strand, within EX31_RS21150 at 873.909 kb on - strand, within EX31_RS21150 at 873.909 kb on - strand, within EX31_RS21150 at 873.909 kb on - strand, within EX31_RS21150 at 873.909 kb on - strand, within EX31_RS21150 at 873.909 kb on - strand, within EX31_RS21150 at 873.909 kb on - strand, within EX31_RS21150 at 873.909 kb on - strand, within EX31_RS21150 at 873.909 kb on - strand, within EX31_RS21150 at 873.910 kb on + strand, within EX31_RS21150 at 873.910 kb on + strand, within EX31_RS21150 at 873.910 kb on + strand, within EX31_RS21150 at 873.910 kb on + strand, within EX31_RS21150 at 873.910 kb on + strand, within EX31_RS21150 at 873.910 kb on + strand, within EX31_RS21150 at 873.910 kb on + strand, within EX31_RS21150 at 873.911 kb on - strand, within EX31_RS21150 at 873.911 kb on - strand, within EX31_RS21150 at 873.911 kb on - strand, within EX31_RS21150 at 873.911 kb on - strand, within EX31_RS21150 at 873.977 kb on - strand, within EX31_RS21150 at 874.020 kb on + strand, within EX31_RS21150 at 874.020 kb on + strand, within EX31_RS21150 at 874.020 kb on + strand, within EX31_RS21150 at 874.051 kb on + strand, within EX31_RS21150 at 874.054 kb on + strand, within EX31_RS21150 at 874.055 kb on - strand, within EX31_RS21150 at 874.055 kb on - strand, within EX31_RS21150 at 874.066 kb on + strand, within EX31_RS21150 at 874.124 kb on + strand, within EX31_RS21150 at 874.126 kb on + strand, within EX31_RS21150 at 874.126 kb on + strand, within EX31_RS21150 at 874.127 kb on - strand, within EX31_RS21150 at 874.127 kb on - strand, within EX31_RS21150 at 874.127 kb on - strand, within EX31_RS21150 at 874.255 kb on + strand, within EX31_RS21150 at 874.255 kb on + strand, within EX31_RS21150 at 874.255 kb on + strand, within EX31_RS21150 at 874.256 kb on - strand, within EX31_RS21150 at 874.333 kb on - strand, within EX31_RS21150 at 874.388 kb on - strand, within EX31_RS21150 at 874.394 kb on - strand, within EX31_RS21150 at 874.490 kb on + strand, within EX31_RS21150 at 874.490 kb on + strand, within EX31_RS21150 at 874.491 kb on - strand, within EX31_RS21150 at 874.491 kb on - strand, within EX31_RS21150 at 874.510 kb on + strand, within EX31_RS21150 at 874.510 kb on + strand, within EX31_RS21150 at 874.510 kb on + strand, within EX31_RS21150 at 874.510 kb on + strand, within EX31_RS21150 at 874.556 kb on + strand at 874.556 kb on + strand at 874.580 kb on - strand at 874.610 kb on + strand at 874.610 kb on + strand at 874.611 kb on - strand at 874.619 kb on - strand at 874.619 kb on - strand at 874.619 kb on - strand at 874.634 kb on + strand at 874.634 kb on + strand at 874.634 kb on + strand at 874.634 kb on + strand at 874.634 kb on + strand at 874.634 kb on + strand at 874.634 kb on + strand at 874.634 kb on + strand at 874.634 kb on + strand at 874.635 kb on - strand at 874.635 kb on - strand at 874.635 kb on - strand at 874.635 kb on - strand at 874.635 kb on - strand at 874.635 kb on - strand at 874.635 kb on - strand at 874.635 kb on - strand at 874.635 kb on - strand at 874.635 kb on - strand at 874.635 kb on - strand at 874.635 kb on - strand at 874.635 kb on - strand at 874.635 kb on - strand at 874.711 kb on - strand, within EX31_RS21155 at 874.784 kb on - strand, within EX31_RS21155 at 874.785 kb on + strand, within EX31_RS21155 at 874.786 kb on - strand, within EX31_RS21155 at 874.786 kb on - strand, within EX31_RS21155 at 874.786 kb on - strand, within EX31_RS21155 at 874.786 kb on - strand, within EX31_RS21155 at 874.786 kb on - strand, within EX31_RS21155 at 874.786 kb on - strand, within EX31_RS21155 at 874.878 kb on + strand, within EX31_RS21155 at 874.878 kb on + strand, within EX31_RS21155 at 874.931 kb on - strand, within EX31_RS21155 at 874.982 kb on + strand, within EX31_RS21155 at 874.993 kb on + strand, within EX31_RS21155 at 874.994 kb on - strand, within EX31_RS21155 at 874.994 kb on - strand, within EX31_RS21155 at 875.154 kb on + strand, within EX31_RS21155 at 875.154 kb on + strand, within EX31_RS21155 at 875.154 kb on + strand, within EX31_RS21155 at 875.154 kb on + strand, within EX31_RS21155 at 875.155 kb on - strand, within EX31_RS21155 at 875.155 kb on - strand, within EX31_RS21155 at 875.155 kb on - strand, within EX31_RS21155 at 875.155 kb on - strand, within EX31_RS21155 at 875.155 kb on - strand, within EX31_RS21155 at 875.155 kb on - strand, within EX31_RS21155 at 875.155 kb on - strand, within EX31_RS21155 at 875.155 kb on - strand, within EX31_RS21155 at 875.157 kb on + strand, within EX31_RS21155 at 875.157 kb on + strand, within EX31_RS21155 at 875.157 kb on + strand, within EX31_RS21155 at 875.157 kb on + strand, within EX31_RS21155 at 875.158 kb on - strand, within EX31_RS21155 at 875.158 kb on - strand, within EX31_RS21155 at 875.158 kb on - strand, within EX31_RS21155 at 875.192 kb on - strand, within EX31_RS21155 at 875.365 kb on + strand at 875.365 kb on + strand at 875.365 kb on + strand at 875.366 kb on - strand at 875.366 kb on - strand at 875.366 kb on - strand at 875.368 kb on - strand at 875.368 kb on - strand at 875.368 kb on - strand at 875.368 kb on - strand at 875.368 kb on - strand at 875.368 kb on - strand at 875.368 kb on - strand at 875.368 kb on - strand at 875.399 kb on + strand at 875.399 kb on + strand at 875.400 kb on - strand at 875.427 kb on + strand at 875.427 kb on + strand at 875.427 kb on + strand at 875.427 kb on + strand at 875.427 kb on + strand at 875.428 kb on - strand at 875.428 kb on - strand at 875.428 kb on - strand at 875.457 kb on - strand at 875.457 kb on - strand at 875.552 kb on + strand, within EX31_RS21160 at 875.553 kb on - strand, within EX31_RS21160 at 875.663 kb on + strand, within EX31_RS21160 at 875.664 kb on - strand, within EX31_RS21160 at 875.664 kb on - strand, within EX31_RS21160 at 875.676 kb on - strand, within EX31_RS21160 at 875.910 kb on + strand, within EX31_RS21160 at 876.008 kb on + strand, within EX31_RS21160 at 876.008 kb on + strand, within EX31_RS21160 at 876.008 kb on + strand, within EX31_RS21160 at 876.008 kb on + strand, within EX31_RS21160 at 876.038 kb on + strand, within EX31_RS21160 at 876.039 kb on - strand, within EX31_RS21160 at 876.058 kb on + strand at 876.058 kb on + strand at 876.058 kb on + strand at 876.058 kb on + strand at 876.059 kb on - strand at 876.062 kb on + strand at 876.062 kb on + strand at 876.062 kb on + strand at 876.062 kb on + strand at 876.062 kb on + strand at 876.062 kb on + strand at 876.062 kb on + strand at 876.063 kb on - strand at 876.063 kb on - strand at 876.063 kb on - strand at 876.063 kb on - strand at 876.063 kb on - strand at 876.063 kb on - strand at 876.063 kb on - strand at 876.063 kb on - strand at 876.063 kb on - strand at 876.075 kb on - strand at 876.089 kb on - strand at 876.089 kb on - strand at 876.096 kb on - strand at 876.098 kb on + strand at 876.098 kb on + strand at 876.098 kb on + strand at 876.099 kb on - strand at 876.232 kb on + strand at 876.278 kb on + strand at 876.296 kb on + strand at 876.296 kb on + strand at 876.363 kb on + strand at 876.364 kb on - strand at 876.364 kb on - strand at 876.364 kb on - strand at 876.364 kb on - strand at 876.364 kb on - strand at 876.364 kb on - strand at 876.366 kb on + strand at 876.366 kb on + strand at 876.367 kb on - strand at 876.383 kb on + strand at 876.384 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N); RCH2 media; shaking remove 873,744 - -0.7 873,775 + +1.4 873,776 - -0.2 873,877 + EX31_RS21150 0.19 +0.2 873,878 - EX31_RS21150 0.19 -0.2 873,878 - EX31_RS21150 0.19 -0.3 873,878 - EX31_RS21150 0.19 -0.1 873,904 + EX31_RS21150 0.22 -0.5 873,904 + EX31_RS21150 0.22 -1.6 873,905 - EX31_RS21150 0.22 -0.5 873,908 + EX31_RS21150 0.23 +1.2 873,908 + EX31_RS21150 0.23 +0.4 873,908 + EX31_RS21150 0.23 -1.0 873,908 + EX31_RS21150 0.23 -1.1 873,908 + EX31_RS21150 0.23 -2.1 873,908 + EX31_RS21150 0.23 +0.1 873,908 + EX31_RS21150 0.23 +1.5 873,909 - EX31_RS21150 0.23 -1.0 873,909 - EX31_RS21150 0.23 -0.4 873,909 - EX31_RS21150 0.23 -1.9 873,909 - EX31_RS21150 0.23 -0.5 873,909 - EX31_RS21150 0.23 +0.5 873,909 - EX31_RS21150 0.23 -0.6 873,909 - EX31_RS21150 0.23 -0.8 873,909 - EX31_RS21150 0.23 -0.1 873,909 - EX31_RS21150 0.23 -1.3 873,910 + EX31_RS21150 0.23 -0.4 873,910 + EX31_RS21150 0.23 -0.4 873,910 + EX31_RS21150 0.23 -1.4 873,910 + EX31_RS21150 0.23 +2.2 873,910 + EX31_RS21150 0.23 -1.3 873,910 + EX31_RS21150 0.23 +0.2 873,910 + EX31_RS21150 0.23 -0.4 873,911 - EX31_RS21150 0.23 +0.3 873,911 - EX31_RS21150 0.23 +2.1 873,911 - EX31_RS21150 0.23 +0.9 873,911 - EX31_RS21150 0.23 -1.5 873,977 - EX31_RS21150 0.30 -0.7 874,020 + EX31_RS21150 0.35 -1.5 874,020 + EX31_RS21150 0.35 +1.1 874,020 + EX31_RS21150 0.35 +0.7 874,051 + EX31_RS21150 0.38 -0.2 874,054 + EX31_RS21150 0.38 -0.0 874,055 - EX31_RS21150 0.38 -0.9 874,055 - EX31_RS21150 0.38 +1.3 874,066 + EX31_RS21150 0.39 +0.4 874,124 + EX31_RS21150 0.46 -0.4 874,126 + EX31_RS21150 0.46 +0.3 874,126 + EX31_RS21150 0.46 -1.9 874,127 - EX31_RS21150 0.46 -0.2 874,127 - EX31_RS21150 0.46 +0.4 874,127 - EX31_RS21150 0.46 +0.2 874,255 + EX31_RS21150 0.60 +1.2 874,255 + EX31_RS21150 0.60 -0.5 874,255 + EX31_RS21150 0.60 -1.0 874,256 - EX31_RS21150 0.60 +0.2 874,333 - EX31_RS21150 0.68 +1.1 874,388 - EX31_RS21150 0.74 -0.4 874,394 - EX31_RS21150 0.74 -0.4 874,490 + EX31_RS21150 0.85 +1.1 874,490 + EX31_RS21150 0.85 -2.4 874,491 - EX31_RS21150 0.85 -0.1 874,491 - EX31_RS21150 0.85 +1.5 874,510 + EX31_RS21150 0.87 +0.3 874,510 + EX31_RS21150 0.87 +0.7 874,510 + EX31_RS21150 0.87 -0.4 874,510 + EX31_RS21150 0.87 +0.1 874,556 + -1.9 874,556 + +0.5 874,580 - +0.6 874,610 + +0.2 874,610 + +1.6 874,611 - +0.9 874,619 - -0.6 874,619 - +0.6 874,619 - -0.6 874,634 + +0.9 874,634 + +0.7 874,634 + +1.3 874,634 + -0.6 874,634 + +0.1 874,634 + +1.1 874,634 + -0.3 874,634 + +0.1 874,634 + -0.3 874,635 - +0.5 874,635 - -0.5 874,635 - -1.0 874,635 - +0.4 874,635 - +0.7 874,635 - -0.4 874,635 - +0.6 874,635 - +0.0 874,635 - -1.2 874,635 - -0.2 874,635 - +0.7 874,635 - -1.8 874,635 - -0.6 874,635 - +1.2 874,711 - EX31_RS21155 0.11 -0.1 874,784 - EX31_RS21155 0.21 +0.8 874,785 + EX31_RS21155 0.21 +1.2 874,786 - EX31_RS21155 0.21 -0.7 874,786 - EX31_RS21155 0.21 -0.1 874,786 - EX31_RS21155 0.21 -0.4 874,786 - EX31_RS21155 0.21 +0.7 874,786 - EX31_RS21155 0.21 -0.4 874,786 - EX31_RS21155 0.21 +0.1 874,878 + EX31_RS21155 0.33 -0.8 874,878 + EX31_RS21155 0.33 +0.4 874,931 - EX31_RS21155 0.40 +1.2 874,982 + EX31_RS21155 0.47 -0.9 874,993 + EX31_RS21155 0.48 +0.2 874,994 - EX31_RS21155 0.48 -0.5 874,994 - EX31_RS21155 0.48 +0.2 875,154 + EX31_RS21155 0.69 +0.1 875,154 + EX31_RS21155 0.69 -0.2 875,154 + EX31_RS21155 0.69 -1.4 875,154 + EX31_RS21155 0.69 -0.5 875,155 - EX31_RS21155 0.70 -0.3 875,155 - EX31_RS21155 0.70 +0.1 875,155 - EX31_RS21155 0.70 +0.2 875,155 - EX31_RS21155 0.70 -2.2 875,155 - EX31_RS21155 0.70 -0.5 875,155 - EX31_RS21155 0.70 +0.3 875,155 - EX31_RS21155 0.70 -0.4 875,155 - EX31_RS21155 0.70 +0.5 875,157 + EX31_RS21155 0.70 -0.3 875,157 + EX31_RS21155 0.70 -0.3 875,157 + EX31_RS21155 0.70 +1.3 875,157 + EX31_RS21155 0.70 +0.4 875,158 - EX31_RS21155 0.70 +0.2 875,158 - EX31_RS21155 0.70 -0.1 875,158 - EX31_RS21155 0.70 +1.1 875,192 - EX31_RS21155 0.74 +0.2 875,365 + -0.6 875,365 + +0.2 875,365 + -0.8 875,366 - +1.1 875,366 - +0.0 875,366 - -1.5 875,368 - -0.1 875,368 - +0.4 875,368 - +0.4 875,368 - +1.1 875,368 - -0.9 875,368 - +0.3 875,368 - -0.5 875,368 - -0.8 875,399 + -0.6 875,399 + +0.3 875,400 - +1.7 875,427 + +0.3 875,427 + -2.3 875,427 + -0.3 875,427 + +1.7 875,427 + +0.7 875,428 - +0.7 875,428 - -0.4 875,428 - -0.0 875,457 - +0.2 875,457 - -0.3 875,552 + EX31_RS21160 0.23 -1.5 875,553 - EX31_RS21160 0.23 +0.1 875,663 + EX31_RS21160 0.38 -0.1 875,664 - EX31_RS21160 0.38 +0.9 875,664 - EX31_RS21160 0.38 +0.1 875,676 - EX31_RS21160 0.40 +0.3 875,910 + EX31_RS21160 0.71 -0.9 876,008 + EX31_RS21160 0.85 -0.1 876,008 + EX31_RS21160 0.85 +0.6 876,008 + EX31_RS21160 0.85 -0.3 876,008 + EX31_RS21160 0.85 +0.1 876,038 + EX31_RS21160 0.89 -0.5 876,039 - EX31_RS21160 0.89 +1.4 876,058 + +0.8 876,058 + +1.5 876,058 + +0.0 876,058 + +0.1 876,059 - +1.6 876,062 + +1.0 876,062 + -0.9 876,062 + -1.0 876,062 + +0.1 876,062 + +1.9 876,062 + -0.1 876,062 + -2.3 876,063 - -0.6 876,063 - -1.8 876,063 - -0.7 876,063 - +0.2 876,063 - -0.1 876,063 - -0.5 876,063 - -0.3 876,063 - -0.2 876,063 - +0.7 876,075 - +0.1 876,089 - +0.4 876,089 - +0.6 876,096 - -0.2 876,098 + -0.6 876,098 + -1.2 876,098 + +0.8 876,099 - -0.1 876,232 + -2.3 876,278 + +0.3 876,296 + +0.3 876,296 + +0.7 876,363 + -1.1 876,364 - -0.1 876,364 - +0.4 876,364 - -0.1 876,364 - -0.5 876,364 - -0.2 876,364 - +1.2 876,366 + +0.7 876,366 + +0.4 876,367 - -0.0 876,383 + -0.2 876,384 - +0.8
Or see this region's nucleotide sequence