Experiment: Ammonium chloride (N); RCH2 media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS18880 and EX31_RS18885 overlap by 8 nucleotides EX31_RS18885 and EX31_RS18890 are separated by 81 nucleotides EX31_RS18890 and EX31_RS18895 are separated by 399 nucleotides
EX31_RS18880: EX31_RS18880 - thioredoxin reductase, at 362,788 to 363,429
_RS18880
EX31_RS18885: EX31_RS18885 - molybdopterin-dependent oxidoreductase, at 363,422 to 364,186
_RS18885
EX31_RS18890: EX31_RS18890 - outer membrane protein OmpX, at 364,268 to 364,801
_RS18890
EX31_RS18895: EX31_RS18895 - threonine/homoserine exporter RhtA, at 365,201 to 366,187
_RS18895
Position (kb) 364
365 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 363.290 kb on - strand, within EX31_RS18880 at 363.333 kb on + strand, within EX31_RS18880 at 363.341 kb on + strand, within EX31_RS18880 at 363.342 kb on - strand, within EX31_RS18880 at 363.487 kb on + strand at 363.505 kb on - strand, within EX31_RS18885 at 363.554 kb on - strand, within EX31_RS18885 at 363.701 kb on + strand, within EX31_RS18885 at 363.714 kb on - strand, within EX31_RS18885 at 363.744 kb on - strand, within EX31_RS18885 at 363.840 kb on + strand, within EX31_RS18885 at 363.932 kb on + strand, within EX31_RS18885 at 363.932 kb on + strand, within EX31_RS18885 at 363.932 kb on + strand, within EX31_RS18885 at 363.932 kb on + strand, within EX31_RS18885 at 363.933 kb on - strand, within EX31_RS18885 at 363.933 kb on - strand, within EX31_RS18885 at 363.933 kb on - strand, within EX31_RS18885 at 363.933 kb on - strand, within EX31_RS18885 at 363.959 kb on + strand, within EX31_RS18885 at 363.963 kb on - strand, within EX31_RS18885 at 363.963 kb on - strand, within EX31_RS18885 at 363.971 kb on - strand, within EX31_RS18885 at 364.091 kb on - strand, within EX31_RS18885 at 364.163 kb on + strand at 364.163 kb on + strand at 364.163 kb on + strand at 364.164 kb on - strand at 364.164 kb on - strand at 364.165 kb on + strand at 364.165 kb on + strand at 364.165 kb on + strand at 364.165 kb on + strand at 364.165 kb on + strand at 364.165 kb on + strand at 364.166 kb on - strand at 364.166 kb on - strand at 364.194 kb on - strand at 364.252 kb on - strand at 364.270 kb on - strand at 364.270 kb on - strand at 364.270 kb on - strand at 364.336 kb on - strand, within EX31_RS18890 at 364.336 kb on - strand, within EX31_RS18890 at 364.336 kb on - strand, within EX31_RS18890 at 364.402 kb on - strand, within EX31_RS18890 at 364.402 kb on - strand, within EX31_RS18890 at 364.430 kb on - strand, within EX31_RS18890 at 364.436 kb on - strand, within EX31_RS18890 at 364.436 kb on - strand, within EX31_RS18890 at 364.436 kb on - strand, within EX31_RS18890 at 364.456 kb on - strand, within EX31_RS18890 at 364.471 kb on - strand, within EX31_RS18890 at 364.492 kb on - strand, within EX31_RS18890 at 364.492 kb on - strand, within EX31_RS18890 at 364.492 kb on - strand, within EX31_RS18890 at 364.492 kb on - strand, within EX31_RS18890 at 364.492 kb on - strand, within EX31_RS18890 at 364.492 kb on - strand, within EX31_RS18890 at 364.543 kb on - strand, within EX31_RS18890 at 364.543 kb on - strand, within EX31_RS18890 at 364.545 kb on + strand, within EX31_RS18890 at 364.546 kb on - strand, within EX31_RS18890 at 364.546 kb on - strand, within EX31_RS18890 at 364.546 kb on - strand, within EX31_RS18890 at 364.546 kb on - strand, within EX31_RS18890 at 364.546 kb on - strand, within EX31_RS18890 at 364.597 kb on - strand, within EX31_RS18890 at 364.597 kb on - strand, within EX31_RS18890 at 364.597 kb on - strand, within EX31_RS18890 at 364.597 kb on - strand, within EX31_RS18890 at 364.631 kb on - strand, within EX31_RS18890 at 364.631 kb on - strand, within EX31_RS18890 at 364.631 kb on - strand, within EX31_RS18890 at 364.631 kb on - strand, within EX31_RS18890 at 364.631 kb on - strand, within EX31_RS18890 at 364.631 kb on - strand, within EX31_RS18890 at 364.632 kb on - strand, within EX31_RS18890 at 364.653 kb on - strand, within EX31_RS18890 at 364.653 kb on - strand, within EX31_RS18890 at 364.716 kb on - strand, within EX31_RS18890 at 364.764 kb on - strand at 364.764 kb on - strand at 364.773 kb on - strand at 364.773 kb on - strand at 364.773 kb on - strand at 364.773 kb on - strand at 364.802 kb on - strand at 364.830 kb on - strand at 364.848 kb on + strand at 364.849 kb on - strand at 364.849 kb on - strand at 364.882 kb on + strand at 364.883 kb on - strand at 364.883 kb on - strand at 364.970 kb on + strand at 365.117 kb on - strand at 365.133 kb on - strand at 365.152 kb on + strand at 365.152 kb on + strand at 365.153 kb on - strand at 365.263 kb on + strand at 365.263 kb on + strand at 365.263 kb on + strand at 365.263 kb on + strand at 365.263 kb on + strand at 365.263 kb on + strand at 365.263 kb on + strand at 365.263 kb on + strand at 365.263 kb on + strand at 365.263 kb on + strand at 365.263 kb on + strand at 365.264 kb on - strand at 365.264 kb on - strand at 365.264 kb on - strand at 365.264 kb on - strand at 365.264 kb on - strand at 365.264 kb on - strand at 365.264 kb on - strand at 365.328 kb on + strand, within EX31_RS18895 at 365.328 kb on + strand, within EX31_RS18895 at 365.328 kb on + strand, within EX31_RS18895 at 365.328 kb on + strand, within EX31_RS18895 at 365.328 kb on + strand, within EX31_RS18895 at 365.328 kb on + strand, within EX31_RS18895 at 365.328 kb on + strand, within EX31_RS18895 at 365.328 kb on + strand, within EX31_RS18895 at 365.349 kb on + strand, within EX31_RS18895 at 365.349 kb on + strand, within EX31_RS18895 at 365.350 kb on - strand, within EX31_RS18895 at 365.350 kb on - strand, within EX31_RS18895 at 365.350 kb on - strand, within EX31_RS18895 at 365.350 kb on - strand, within EX31_RS18895 at 365.450 kb on + strand, within EX31_RS18895 at 365.451 kb on - strand, within EX31_RS18895 at 365.507 kb on + strand, within EX31_RS18895 at 365.507 kb on + strand, within EX31_RS18895 at 365.507 kb on + strand, within EX31_RS18895 at 365.507 kb on + strand, within EX31_RS18895 at 365.507 kb on + strand, within EX31_RS18895 at 365.507 kb on + strand, within EX31_RS18895 at 365.507 kb on + strand, within EX31_RS18895 at 365.507 kb on + strand, within EX31_RS18895 at 365.507 kb on + strand, within EX31_RS18895 at 365.507 kb on + strand, within EX31_RS18895 at 365.508 kb on - strand, within EX31_RS18895 at 365.508 kb on - strand, within EX31_RS18895 at 365.508 kb on - strand, within EX31_RS18895 at 365.508 kb on - strand, within EX31_RS18895 at 365.534 kb on + strand, within EX31_RS18895 at 365.534 kb on + strand, within EX31_RS18895 at 365.534 kb on + strand, within EX31_RS18895 at 365.534 kb on + strand, within EX31_RS18895 at 365.534 kb on + strand, within EX31_RS18895 at 365.534 kb on + strand, within EX31_RS18895 at 365.534 kb on + strand, within EX31_RS18895 at 365.535 kb on - strand, within EX31_RS18895 at 365.543 kb on + strand, within EX31_RS18895 at 365.544 kb on - strand, within EX31_RS18895 at 365.765 kb on + strand, within EX31_RS18895 at 365.765 kb on + strand, within EX31_RS18895 at 365.765 kb on + strand, within EX31_RS18895 at 365.765 kb on + strand, within EX31_RS18895 at 365.765 kb on + strand, within EX31_RS18895 at 365.766 kb on - strand, within EX31_RS18895 at 365.766 kb on - strand, within EX31_RS18895
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N); RCH2 media; shaking remove 363,290 - EX31_RS18880 0.78 -1.6 363,333 + EX31_RS18880 0.85 -0.5 363,341 + EX31_RS18880 0.86 +0.3 363,342 - EX31_RS18880 0.86 +1.1 363,487 + +0.3 363,505 - EX31_RS18885 0.11 +0.8 363,554 - EX31_RS18885 0.17 +0.3 363,701 + EX31_RS18885 0.36 -0.5 363,714 - EX31_RS18885 0.38 -0.2 363,744 - EX31_RS18885 0.42 +0.5 363,840 + EX31_RS18885 0.55 +1.7 363,932 + EX31_RS18885 0.67 +1.7 363,932 + EX31_RS18885 0.67 +0.5 363,932 + EX31_RS18885 0.67 -1.7 363,932 + EX31_RS18885 0.67 +0.8 363,933 - EX31_RS18885 0.67 +1.2 363,933 - EX31_RS18885 0.67 +0.0 363,933 - EX31_RS18885 0.67 +0.0 363,933 - EX31_RS18885 0.67 +1.4 363,959 + EX31_RS18885 0.70 +0.8 363,963 - EX31_RS18885 0.71 +0.2 363,963 - EX31_RS18885 0.71 +1.2 363,971 - EX31_RS18885 0.72 +1.3 364,091 - EX31_RS18885 0.87 +0.6 364,163 + -1.1 364,163 + +0.5 364,163 + -0.2 364,164 - -1.6 364,164 - -0.4 364,165 + -0.6 364,165 + -0.3 364,165 + +2.2 364,165 + +2.8 364,165 + -0.5 364,165 + +0.6 364,166 - -0.6 364,166 - +1.0 364,194 - +0.1 364,252 - +0.3 364,270 - -0.2 364,270 - +0.7 364,270 - +0.1 364,336 - EX31_RS18890 0.13 -0.7 364,336 - EX31_RS18890 0.13 +0.8 364,336 - EX31_RS18890 0.13 -0.5 364,402 - EX31_RS18890 0.25 +1.3 364,402 - EX31_RS18890 0.25 -1.4 364,430 - EX31_RS18890 0.30 +0.9 364,436 - EX31_RS18890 0.31 -0.1 364,436 - EX31_RS18890 0.31 +0.6 364,436 - EX31_RS18890 0.31 +0.1 364,456 - EX31_RS18890 0.35 +1.3 364,471 - EX31_RS18890 0.38 -0.0 364,492 - EX31_RS18890 0.42 +0.7 364,492 - EX31_RS18890 0.42 +0.2 364,492 - EX31_RS18890 0.42 +0.1 364,492 - EX31_RS18890 0.42 -2.0 364,492 - EX31_RS18890 0.42 +0.8 364,492 - EX31_RS18890 0.42 +0.4 364,543 - EX31_RS18890 0.51 +1.5 364,543 - EX31_RS18890 0.51 +0.5 364,545 + EX31_RS18890 0.52 +0.4 364,546 - EX31_RS18890 0.52 -0.1 364,546 - EX31_RS18890 0.52 +0.1 364,546 - EX31_RS18890 0.52 +1.1 364,546 - EX31_RS18890 0.52 +0.0 364,546 - EX31_RS18890 0.52 +0.3 364,597 - EX31_RS18890 0.62 -0.9 364,597 - EX31_RS18890 0.62 -0.5 364,597 - EX31_RS18890 0.62 +0.1 364,597 - EX31_RS18890 0.62 +0.9 364,631 - EX31_RS18890 0.68 -1.2 364,631 - EX31_RS18890 0.68 -1.0 364,631 - EX31_RS18890 0.68 +0.5 364,631 - EX31_RS18890 0.68 +0.5 364,631 - EX31_RS18890 0.68 +0.3 364,631 - EX31_RS18890 0.68 +1.0 364,632 - EX31_RS18890 0.68 +1.4 364,653 - EX31_RS18890 0.72 -0.0 364,653 - EX31_RS18890 0.72 -0.6 364,716 - EX31_RS18890 0.84 +0.0 364,764 - -0.6 364,764 - -0.5 364,773 - -0.4 364,773 - +0.5 364,773 - +0.6 364,773 - +1.2 364,802 - +0.1 364,830 - +0.2 364,848 + -0.7 364,849 - -1.5 364,849 - +1.8 364,882 + +0.4 364,883 - -0.1 364,883 - -1.0 364,970 + +0.7 365,117 - +0.1 365,133 - +0.8 365,152 + +0.3 365,152 + -0.5 365,153 - +1.3 365,263 + -1.1 365,263 + -1.2 365,263 + +0.4 365,263 + +2.7 365,263 + -0.2 365,263 + +0.6 365,263 + -0.0 365,263 + +0.2 365,263 + +1.1 365,263 + -0.3 365,263 + -0.2 365,264 - -0.7 365,264 - -0.9 365,264 - -0.2 365,264 - +2.3 365,264 - -0.8 365,264 - -1.7 365,264 - +0.7 365,328 + EX31_RS18895 0.13 +0.3 365,328 + EX31_RS18895 0.13 -0.2 365,328 + EX31_RS18895 0.13 -1.9 365,328 + EX31_RS18895 0.13 +0.8 365,328 + EX31_RS18895 0.13 -0.7 365,328 + EX31_RS18895 0.13 +0.5 365,328 + EX31_RS18895 0.13 +1.6 365,328 + EX31_RS18895 0.13 +1.4 365,349 + EX31_RS18895 0.15 -0.1 365,349 + EX31_RS18895 0.15 -0.2 365,350 - EX31_RS18895 0.15 +0.8 365,350 - EX31_RS18895 0.15 +1.3 365,350 - EX31_RS18895 0.15 -0.4 365,350 - EX31_RS18895 0.15 +0.3 365,450 + EX31_RS18895 0.25 +0.5 365,451 - EX31_RS18895 0.25 -0.8 365,507 + EX31_RS18895 0.31 +0.8 365,507 + EX31_RS18895 0.31 -0.4 365,507 + EX31_RS18895 0.31 +0.7 365,507 + EX31_RS18895 0.31 -0.2 365,507 + EX31_RS18895 0.31 +0.7 365,507 + EX31_RS18895 0.31 -0.7 365,507 + EX31_RS18895 0.31 -0.2 365,507 + EX31_RS18895 0.31 +0.1 365,507 + EX31_RS18895 0.31 -0.2 365,507 + EX31_RS18895 0.31 +0.2 365,508 - EX31_RS18895 0.31 -0.9 365,508 - EX31_RS18895 0.31 -0.4 365,508 - EX31_RS18895 0.31 -0.5 365,508 - EX31_RS18895 0.31 +0.3 365,534 + EX31_RS18895 0.34 -0.0 365,534 + EX31_RS18895 0.34 +1.8 365,534 + EX31_RS18895 0.34 +0.3 365,534 + EX31_RS18895 0.34 -0.3 365,534 + EX31_RS18895 0.34 +0.2 365,534 + EX31_RS18895 0.34 +0.4 365,534 + EX31_RS18895 0.34 +1.1 365,535 - EX31_RS18895 0.34 +0.8 365,543 + EX31_RS18895 0.35 +0.5 365,544 - EX31_RS18895 0.35 -0.1 365,765 + EX31_RS18895 0.57 -0.5 365,765 + EX31_RS18895 0.57 +0.0 365,765 + EX31_RS18895 0.57 +1.5 365,765 + EX31_RS18895 0.57 -0.6 365,765 + EX31_RS18895 0.57 -0.2 365,766 - EX31_RS18895 0.57 -1.3 365,766 - EX31_RS18895 0.57 +0.7
Or see this region's nucleotide sequence