Experiment: Ammonium chloride (N); RCH2 media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS14325 and EX31_RS14330 are separated by 37 nucleotides EX31_RS14330 and EX31_RS14335 are separated by 52 nucleotides EX31_RS14335 and EX31_RS14340 are separated by 87 nucleotides
EX31_RS14325: EX31_RS14325 - envelope stress sensor histidine kinase CpxA, at 46,584 to 47,954
_RS14325
EX31_RS14330: EX31_RS14330 - tRNA (uridine(34)/cytosine(34)/5- carboxymethylaminomethyluridine(34)-2'-O)- methyltransferase TrmL, at 47,992 to 48,465
_RS14330
EX31_RS14335: EX31_RS14335 - serine O-acetyltransferase, at 48,518 to 49,339
_RS14335
EX31_RS14340: EX31_RS14340 - NAD(P)H-dependent glycerol-3-phosphate dehydrogenase, at 49,427 to 50,446
_RS14340
Position (kb)
48
49
50 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 47.727 kb on - strand, within EX31_RS14325 at 47.887 kb on + strand at 47.888 kb on - strand at 47.888 kb on - strand at 47.963 kb on + strand at 47.964 kb on - strand at 47.978 kb on + strand at 47.979 kb on - strand at 47.997 kb on + strand at 47.997 kb on + strand at 47.997 kb on + strand at 47.997 kb on + strand at 47.997 kb on + strand at 47.998 kb on - strand at 47.998 kb on - strand at 47.998 kb on - strand at 47.998 kb on - strand at 48.008 kb on + strand at 48.008 kb on + strand at 48.009 kb on - strand at 48.143 kb on - strand, within EX31_RS14330 at 48.143 kb on - strand, within EX31_RS14330 at 48.159 kb on + strand, within EX31_RS14330 at 48.160 kb on - strand, within EX31_RS14330 at 48.161 kb on + strand, within EX31_RS14330 at 48.161 kb on + strand, within EX31_RS14330 at 48.161 kb on + strand, within EX31_RS14330 at 48.161 kb on + strand, within EX31_RS14330 at 48.161 kb on + strand, within EX31_RS14330 at 48.161 kb on + strand, within EX31_RS14330 at 48.161 kb on + strand, within EX31_RS14330 at 48.161 kb on + strand, within EX31_RS14330 at 48.161 kb on + strand, within EX31_RS14330 at 48.161 kb on + strand, within EX31_RS14330 at 48.161 kb on + strand, within EX31_RS14330 at 48.161 kb on + strand, within EX31_RS14330 at 48.161 kb on + strand, within EX31_RS14330 at 48.161 kb on + strand, within EX31_RS14330 at 48.161 kb on + strand, within EX31_RS14330 at 48.161 kb on + strand, within EX31_RS14330 at 48.161 kb on + strand, within EX31_RS14330 at 48.161 kb on + strand, within EX31_RS14330 at 48.161 kb on + strand, within EX31_RS14330 at 48.162 kb on - strand, within EX31_RS14330 at 48.162 kb on - strand, within EX31_RS14330 at 48.162 kb on - strand, within EX31_RS14330 at 48.162 kb on - strand, within EX31_RS14330 at 48.162 kb on - strand, within EX31_RS14330 at 48.162 kb on - strand, within EX31_RS14330 at 48.162 kb on - strand, within EX31_RS14330 at 48.162 kb on - strand, within EX31_RS14330 at 48.162 kb on - strand, within EX31_RS14330 at 48.162 kb on - strand, within EX31_RS14330 at 48.162 kb on - strand, within EX31_RS14330 at 48.162 kb on - strand, within EX31_RS14330 at 48.162 kb on - strand, within EX31_RS14330 at 48.162 kb on - strand, within EX31_RS14330 at 48.178 kb on + strand, within EX31_RS14330 at 48.178 kb on + strand, within EX31_RS14330 at 48.178 kb on + strand, within EX31_RS14330 at 48.178 kb on + strand, within EX31_RS14330 at 48.179 kb on - strand, within EX31_RS14330 at 48.179 kb on - strand, within EX31_RS14330 at 48.179 kb on - strand, within EX31_RS14330 at 48.262 kb on + strand, within EX31_RS14330 at 48.262 kb on + strand, within EX31_RS14330 at 48.263 kb on - strand, within EX31_RS14330 at 48.263 kb on - strand, within EX31_RS14330 at 48.263 kb on - strand, within EX31_RS14330 at 48.318 kb on + strand, within EX31_RS14330 at 48.319 kb on - strand, within EX31_RS14330 at 48.319 kb on - strand, within EX31_RS14330 at 48.319 kb on - strand, within EX31_RS14330 at 48.319 kb on - strand, within EX31_RS14330 at 48.319 kb on - strand, within EX31_RS14330 at 48.360 kb on + strand, within EX31_RS14330 at 48.361 kb on - strand, within EX31_RS14330 at 48.415 kb on + strand, within EX31_RS14330 at 48.415 kb on + strand, within EX31_RS14330 at 48.416 kb on - strand, within EX31_RS14330 at 48.416 kb on - strand, within EX31_RS14330 at 48.464 kb on - strand at 48.553 kb on + strand at 48.553 kb on + strand at 48.554 kb on - strand at 48.574 kb on + strand at 48.575 kb on - strand at 48.707 kb on - strand, within EX31_RS14335 at 48.707 kb on - strand, within EX31_RS14335 at 48.757 kb on + strand, within EX31_RS14335 at 48.788 kb on - strand, within EX31_RS14335 at 48.819 kb on - strand, within EX31_RS14335 at 48.910 kb on + strand, within EX31_RS14335 at 48.911 kb on - strand, within EX31_RS14335 at 48.914 kb on + strand, within EX31_RS14335 at 48.995 kb on - strand, within EX31_RS14335 at 49.048 kb on - strand, within EX31_RS14335 at 49.200 kb on + strand, within EX31_RS14335 at 49.200 kb on + strand, within EX31_RS14335 at 49.201 kb on - strand, within EX31_RS14335 at 49.300 kb on + strand at 49.300 kb on + strand at 49.301 kb on - strand at 49.306 kb on + strand at 49.322 kb on + strand at 49.323 kb on - strand at 49.396 kb on - strand at 49.550 kb on - strand, within EX31_RS14340 at 49.727 kb on - strand, within EX31_RS14340 at 50.187 kb on - strand, within EX31_RS14340 at 50.216 kb on - strand, within EX31_RS14340 at 50.316 kb on - strand, within EX31_RS14340
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N); RCH2 media; shaking remove 47,727 - EX31_RS14325 0.83 -0.2 47,887 + +1.1 47,888 - -0.3 47,888 - +1.0 47,963 + -1.6 47,964 - +0.3 47,978 + -1.0 47,979 - +0.1 47,997 + +0.4 47,997 + -0.2 47,997 + +0.1 47,997 + +0.6 47,997 + +0.2 47,998 - -0.3 47,998 - -1.3 47,998 - +0.2 47,998 - +0.7 48,008 + -0.2 48,008 + +0.7 48,009 - -0.3 48,143 - EX31_RS14330 0.32 -0.2 48,143 - EX31_RS14330 0.32 +0.1 48,159 + EX31_RS14330 0.35 -0.0 48,160 - EX31_RS14330 0.35 +0.9 48,161 + EX31_RS14330 0.36 +1.0 48,161 + EX31_RS14330 0.36 +0.3 48,161 + EX31_RS14330 0.36 -0.3 48,161 + EX31_RS14330 0.36 +1.6 48,161 + EX31_RS14330 0.36 +0.6 48,161 + EX31_RS14330 0.36 +1.0 48,161 + EX31_RS14330 0.36 +1.3 48,161 + EX31_RS14330 0.36 +0.2 48,161 + EX31_RS14330 0.36 +1.0 48,161 + EX31_RS14330 0.36 +1.3 48,161 + EX31_RS14330 0.36 +1.2 48,161 + EX31_RS14330 0.36 +0.4 48,161 + EX31_RS14330 0.36 +0.0 48,161 + EX31_RS14330 0.36 +1.4 48,161 + EX31_RS14330 0.36 -0.6 48,161 + EX31_RS14330 0.36 +0.2 48,161 + EX31_RS14330 0.36 +0.3 48,161 + EX31_RS14330 0.36 +0.3 48,161 + EX31_RS14330 0.36 +0.5 48,162 - EX31_RS14330 0.36 +0.8 48,162 - EX31_RS14330 0.36 +1.2 48,162 - EX31_RS14330 0.36 -0.4 48,162 - EX31_RS14330 0.36 +0.7 48,162 - EX31_RS14330 0.36 -0.7 48,162 - EX31_RS14330 0.36 -0.1 48,162 - EX31_RS14330 0.36 -1.5 48,162 - EX31_RS14330 0.36 +0.8 48,162 - EX31_RS14330 0.36 -0.7 48,162 - EX31_RS14330 0.36 +0.2 48,162 - EX31_RS14330 0.36 -1.1 48,162 - EX31_RS14330 0.36 -0.9 48,162 - EX31_RS14330 0.36 +0.7 48,162 - EX31_RS14330 0.36 -0.0 48,178 + EX31_RS14330 0.39 -0.6 48,178 + EX31_RS14330 0.39 +0.5 48,178 + EX31_RS14330 0.39 -0.4 48,178 + EX31_RS14330 0.39 +0.8 48,179 - EX31_RS14330 0.39 +1.0 48,179 - EX31_RS14330 0.39 +0.6 48,179 - EX31_RS14330 0.39 +0.8 48,262 + EX31_RS14330 0.57 -0.3 48,262 + EX31_RS14330 0.57 +1.3 48,263 - EX31_RS14330 0.57 -0.2 48,263 - EX31_RS14330 0.57 +0.6 48,263 - EX31_RS14330 0.57 -0.6 48,318 + EX31_RS14330 0.69 -0.4 48,319 - EX31_RS14330 0.69 +0.4 48,319 - EX31_RS14330 0.69 +0.4 48,319 - EX31_RS14330 0.69 +0.2 48,319 - EX31_RS14330 0.69 +0.1 48,319 - EX31_RS14330 0.69 +0.8 48,360 + EX31_RS14330 0.78 -1.0 48,361 - EX31_RS14330 0.78 -1.0 48,415 + EX31_RS14330 0.89 -0.0 48,415 + EX31_RS14330 0.89 +0.3 48,416 - EX31_RS14330 0.89 -0.2 48,416 - EX31_RS14330 0.89 +1.0 48,464 - -2.2 48,553 + -3.6 48,553 + -2.9 48,554 - -0.9 48,574 + -1.8 48,575 - -0.0 48,707 - EX31_RS14335 0.23 +1.0 48,707 - EX31_RS14335 0.23 -0.6 48,757 + EX31_RS14335 0.29 +1.5 48,788 - EX31_RS14335 0.33 +0.5 48,819 - EX31_RS14335 0.37 -0.3 48,910 + EX31_RS14335 0.48 +0.2 48,911 - EX31_RS14335 0.48 +0.2 48,914 + EX31_RS14335 0.48 -0.8 48,995 - EX31_RS14335 0.58 -1.8 49,048 - EX31_RS14335 0.64 -2.1 49,200 + EX31_RS14335 0.83 -0.5 49,200 + EX31_RS14335 0.83 +0.5 49,201 - EX31_RS14335 0.83 -1.5 49,300 + +2.0 49,300 + -3.0 49,301 - -0.0 49,306 + -2.8 49,322 + -1.8 49,323 - -0.6 49,396 - +0.3 49,550 - EX31_RS14340 0.12 +0.5 49,727 - EX31_RS14340 0.29 -0.5 50,187 - EX31_RS14340 0.75 -0.0 50,216 - EX31_RS14340 0.77 -0.9 50,316 - EX31_RS14340 0.87 -1.2
Or see this region's nucleotide sequence