Experiment: Ammonium chloride (N); RCH2 media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS14200 and EX31_RS14205 are separated by 72 nucleotides EX31_RS14205 and EX31_RS14210 are separated by 247 nucleotides
EX31_RS14200: EX31_RS14200 - phosphoenolpyruvate carboxylase, at 16,979 to 19,624
_RS14200
EX31_RS14205: EX31_RS14205 - methylenetetrahydrofolate reductase, at 19,697 to 20,605
_RS14205
EX31_RS14210: EX31_RS14210 - bifunctional aspartate kinase/homoserine dehydrogenase II, at 20,853 to 23,288
_RS14210
Position (kb) 19
20
21 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3 at 18.806 kb on - strand, within EX31_RS14200 at 18.875 kb on + strand, within EX31_RS14200 at 18.875 kb on + strand, within EX31_RS14200 at 18.876 kb on - strand, within EX31_RS14200 at 18.876 kb on - strand, within EX31_RS14200 at 18.876 kb on - strand, within EX31_RS14200 at 18.876 kb on - strand, within EX31_RS14200 at 18.986 kb on + strand, within EX31_RS14200 at 18.986 kb on + strand, within EX31_RS14200 at 19.016 kb on + strand, within EX31_RS14200 at 19.016 kb on + strand, within EX31_RS14200 at 19.016 kb on + strand, within EX31_RS14200 at 19.017 kb on - strand, within EX31_RS14200 at 19.085 kb on - strand, within EX31_RS14200 at 19.270 kb on + strand, within EX31_RS14200 at 19.316 kb on + strand, within EX31_RS14200 at 19.317 kb on - strand, within EX31_RS14200 at 19.319 kb on + strand, within EX31_RS14200 at 19.319 kb on + strand, within EX31_RS14200 at 19.319 kb on + strand, within EX31_RS14200 at 19.320 kb on - strand, within EX31_RS14200 at 19.320 kb on - strand, within EX31_RS14200 at 19.320 kb on - strand, within EX31_RS14200 at 19.414 kb on + strand at 19.415 kb on - strand at 19.478 kb on + strand at 19.478 kb on + strand at 19.479 kb on - strand at 19.479 kb on - strand at 19.612 kb on + strand at 19.613 kb on - strand at 19.613 kb on - strand at 19.613 kb on - strand at 19.615 kb on + strand at 19.615 kb on + strand at 19.616 kb on - strand at 19.675 kb on + strand at 19.698 kb on + strand at 19.960 kb on + strand, within EX31_RS14205 at 20.034 kb on + strand, within EX31_RS14205 at 20.093 kb on - strand, within EX31_RS14205 at 20.105 kb on - strand, within EX31_RS14205 at 20.105 kb on - strand, within EX31_RS14205 at 20.167 kb on + strand, within EX31_RS14205 at 20.167 kb on + strand, within EX31_RS14205 at 20.168 kb on - strand, within EX31_RS14205 at 20.168 kb on - strand, within EX31_RS14205 at 20.214 kb on + strand, within EX31_RS14205 at 20.214 kb on + strand, within EX31_RS14205 at 20.214 kb on + strand, within EX31_RS14205 at 20.214 kb on + strand, within EX31_RS14205 at 20.214 kb on + strand, within EX31_RS14205 at 20.215 kb on - strand, within EX31_RS14205 at 20.215 kb on - strand, within EX31_RS14205 at 20.290 kb on - strand, within EX31_RS14205 at 20.332 kb on + strand, within EX31_RS14205 at 20.333 kb on - strand, within EX31_RS14205 at 20.333 kb on - strand, within EX31_RS14205 at 20.374 kb on + strand, within EX31_RS14205 at 20.399 kb on - strand, within EX31_RS14205 at 20.446 kb on + strand, within EX31_RS14205 at 20.447 kb on - strand, within EX31_RS14205 at 20.473 kb on + strand, within EX31_RS14205 at 20.473 kb on + strand, within EX31_RS14205 at 20.474 kb on - strand, within EX31_RS14205 at 20.474 kb on - strand, within EX31_RS14205 at 20.474 kb on - strand, within EX31_RS14205 at 20.474 kb on - strand, within EX31_RS14205 at 20.474 kb on - strand, within EX31_RS14205 at 20.474 kb on - strand, within EX31_RS14205 at 20.488 kb on + strand, within EX31_RS14205 at 20.488 kb on + strand, within EX31_RS14205 at 20.488 kb on + strand, within EX31_RS14205 at 20.488 kb on + strand, within EX31_RS14205 at 20.488 kb on + strand, within EX31_RS14205 at 20.488 kb on + strand, within EX31_RS14205 at 20.488 kb on + strand, within EX31_RS14205 at 20.488 kb on + strand, within EX31_RS14205 at 20.489 kb on - strand, within EX31_RS14205 at 20.489 kb on - strand, within EX31_RS14205 at 20.489 kb on - strand, within EX31_RS14205 at 20.489 kb on - strand, within EX31_RS14205 at 20.489 kb on - strand, within EX31_RS14205 at 20.489 kb on - strand, within EX31_RS14205 at 20.489 kb on - strand, within EX31_RS14205 at 20.506 kb on + strand, within EX31_RS14205 at 20.507 kb on - strand, within EX31_RS14205 at 20.549 kb on + strand at 20.549 kb on + strand at 20.549 kb on + strand at 20.550 kb on - strand at 20.605 kb on + strand at 20.605 kb on + strand at 20.606 kb on - strand at 20.612 kb on + strand at 20.612 kb on + strand at 20.639 kb on - strand at 20.639 kb on - strand at 20.639 kb on - strand at 20.676 kb on - strand at 20.678 kb on - strand at 20.716 kb on + strand at 20.717 kb on - strand at 20.717 kb on - strand at 20.738 kb on + strand at 20.738 kb on + strand at 20.739 kb on - strand at 20.739 kb on - strand at 20.739 kb on - strand at 20.817 kb on - strand at 20.854 kb on + strand at 20.854 kb on + strand at 20.855 kb on - strand at 20.876 kb on + strand at 20.876 kb on + strand at 20.876 kb on + strand at 20.953 kb on + strand at 21.073 kb on + strand at 21.074 kb on - strand at 21.074 kb on - strand at 21.074 kb on - strand at 21.162 kb on + strand, within EX31_RS14210 at 21.162 kb on + strand, within EX31_RS14210 at 21.162 kb on + strand, within EX31_RS14210 at 21.162 kb on + strand, within EX31_RS14210 at 21.163 kb on - strand, within EX31_RS14210 at 21.163 kb on - strand, within EX31_RS14210 at 21.163 kb on - strand, within EX31_RS14210 at 21.163 kb on - strand, within EX31_RS14210 at 21.230 kb on - strand, within EX31_RS14210 at 21.283 kb on - strand, within EX31_RS14210 at 21.328 kb on - strand, within EX31_RS14210 at 21.328 kb on - strand, within EX31_RS14210 at 21.438 kb on + strand, within EX31_RS14210 at 21.439 kb on - strand, within EX31_RS14210 at 21.452 kb on + strand, within EX31_RS14210 at 21.452 kb on + strand, within EX31_RS14210 at 21.452 kb on + strand, within EX31_RS14210 at 21.453 kb on - strand, within EX31_RS14210 at 21.453 kb on - strand, within EX31_RS14210 at 21.453 kb on - strand, within EX31_RS14210 at 21.453 kb on - strand, within EX31_RS14210 at 21.453 kb on - strand, within EX31_RS14210 at 21.453 kb on - strand, within EX31_RS14210 at 21.453 kb on - strand, within EX31_RS14210 at 21.489 kb on - strand, within EX31_RS14210 at 21.490 kb on + strand, within EX31_RS14210 at 21.490 kb on + strand, within EX31_RS14210 at 21.490 kb on + strand, within EX31_RS14210 at 21.490 kb on + strand, within EX31_RS14210 at 21.490 kb on + strand, within EX31_RS14210 at 21.490 kb on + strand, within EX31_RS14210 at 21.490 kb on + strand, within EX31_RS14210 at 21.490 kb on + strand, within EX31_RS14210 at 21.491 kb on - strand, within EX31_RS14210 at 21.491 kb on - strand, within EX31_RS14210 at 21.491 kb on - strand, within EX31_RS14210 at 21.491 kb on - strand, within EX31_RS14210 at 21.491 kb on - strand, within EX31_RS14210 at 21.491 kb on - strand, within EX31_RS14210 at 21.491 kb on - strand, within EX31_RS14210 at 21.491 kb on - strand, within EX31_RS14210 at 21.491 kb on - strand, within EX31_RS14210 at 21.538 kb on + strand, within EX31_RS14210 at 21.539 kb on - strand, within EX31_RS14210 at 21.542 kb on + strand, within EX31_RS14210 at 21.542 kb on + strand, within EX31_RS14210 at 21.542 kb on + strand, within EX31_RS14210 at 21.542 kb on + strand, within EX31_RS14210 at 21.542 kb on + strand, within EX31_RS14210 at 21.543 kb on - strand, within EX31_RS14210 at 21.543 kb on - strand, within EX31_RS14210 at 21.595 kb on + strand, within EX31_RS14210 at 21.595 kb on + strand, within EX31_RS14210 at 21.595 kb on + strand, within EX31_RS14210 at 21.596 kb on - strand, within EX31_RS14210 at 21.596 kb on - strand, within EX31_RS14210 at 21.596 kb on - strand, within EX31_RS14210
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N); RCH2 media; shaking remove 18,806 - EX31_RS14200 0.69 -1.9 18,875 + EX31_RS14200 0.72 -3.2 18,875 + EX31_RS14200 0.72 -3.3 18,876 - EX31_RS14200 0.72 -3.5 18,876 - EX31_RS14200 0.72 -3.0 18,876 - EX31_RS14200 0.72 -2.9 18,876 - EX31_RS14200 0.72 -3.3 18,986 + EX31_RS14200 0.76 -3.6 18,986 + EX31_RS14200 0.76 -1.5 19,016 + EX31_RS14200 0.77 -2.9 19,016 + EX31_RS14200 0.77 -5.0 19,016 + EX31_RS14200 0.77 -1.7 19,017 - EX31_RS14200 0.77 +0.5 19,085 - EX31_RS14200 0.80 -1.9 19,270 + EX31_RS14200 0.87 -2.5 19,316 + EX31_RS14200 0.88 -2.9 19,317 - EX31_RS14200 0.88 -2.9 19,319 + EX31_RS14200 0.88 -3.2 19,319 + EX31_RS14200 0.88 -3.7 19,319 + EX31_RS14200 0.88 -2.7 19,320 - EX31_RS14200 0.88 -5.0 19,320 - EX31_RS14200 0.88 -1.0 19,320 - EX31_RS14200 0.88 -3.9 19,414 + -3.9 19,415 - -1.0 19,478 + -1.2 19,478 + -2.9 19,479 - -2.7 19,479 - -2.0 19,612 + -2.7 19,613 - -2.3 19,613 - -1.9 19,613 - -2.7 19,615 + -2.4 19,615 + -3.2 19,616 - -3.0 19,675 + +0.8 19,698 + +1.3 19,960 + EX31_RS14205 0.29 -3.4 20,034 + EX31_RS14205 0.37 -3.9 20,093 - EX31_RS14205 0.44 -4.0 20,105 - EX31_RS14205 0.45 -3.1 20,105 - EX31_RS14205 0.45 -2.4 20,167 + EX31_RS14205 0.52 -4.2 20,167 + EX31_RS14205 0.52 -0.9 20,168 - EX31_RS14205 0.52 -3.6 20,168 - EX31_RS14205 0.52 -2.9 20,214 + EX31_RS14205 0.57 -2.8 20,214 + EX31_RS14205 0.57 -2.7 20,214 + EX31_RS14205 0.57 -1.4 20,214 + EX31_RS14205 0.57 -2.0 20,214 + EX31_RS14205 0.57 +0.2 20,215 - EX31_RS14205 0.57 -3.1 20,215 - EX31_RS14205 0.57 -2.4 20,290 - EX31_RS14205 0.65 -2.4 20,332 + EX31_RS14205 0.70 -3.0 20,333 - EX31_RS14205 0.70 -2.7 20,333 - EX31_RS14205 0.70 -2.6 20,374 + EX31_RS14205 0.74 -3.9 20,399 - EX31_RS14205 0.77 -0.8 20,446 + EX31_RS14205 0.82 +0.7 20,447 - EX31_RS14205 0.83 -3.5 20,473 + EX31_RS14205 0.85 -2.7 20,473 + EX31_RS14205 0.85 -3.8 20,474 - EX31_RS14205 0.85 -2.3 20,474 - EX31_RS14205 0.85 -3.0 20,474 - EX31_RS14205 0.85 -3.4 20,474 - EX31_RS14205 0.85 -2.6 20,474 - EX31_RS14205 0.85 -3.5 20,474 - EX31_RS14205 0.85 -3.7 20,488 + EX31_RS14205 0.87 -2.7 20,488 + EX31_RS14205 0.87 -2.4 20,488 + EX31_RS14205 0.87 -5.0 20,488 + EX31_RS14205 0.87 -0.5 20,488 + EX31_RS14205 0.87 -1.9 20,488 + EX31_RS14205 0.87 -3.0 20,488 + EX31_RS14205 0.87 -0.8 20,488 + EX31_RS14205 0.87 -2.6 20,489 - EX31_RS14205 0.87 -3.8 20,489 - EX31_RS14205 0.87 -2.9 20,489 - EX31_RS14205 0.87 -2.9 20,489 - EX31_RS14205 0.87 -2.3 20,489 - EX31_RS14205 0.87 -3.3 20,489 - EX31_RS14205 0.87 -2.4 20,489 - EX31_RS14205 0.87 -2.4 20,506 + EX31_RS14205 0.89 -1.5 20,507 - EX31_RS14205 0.89 -3.2 20,549 + -4.1 20,549 + -2.8 20,549 + -2.8 20,550 - -2.2 20,605 + -1.8 20,605 + -2.4 20,606 - -3.7 20,612 + -3.9 20,612 + -3.4 20,639 - +1.7 20,639 - +1.5 20,639 - +0.1 20,676 - +0.8 20,678 - +0.3 20,716 + -0.2 20,717 - +1.5 20,717 - +0.5 20,738 + +0.3 20,738 + -0.6 20,739 - +0.4 20,739 - +1.5 20,739 - +0.7 20,817 - +0.6 20,854 + +1.6 20,854 + -0.1 20,855 - +0.3 20,876 + +0.1 20,876 + +1.0 20,876 + -0.4 20,953 + -0.2 21,073 + +0.4 21,074 - +0.3 21,074 - +0.0 21,074 - -1.0 21,162 + EX31_RS14210 0.13 +1.2 21,162 + EX31_RS14210 0.13 +1.4 21,162 + EX31_RS14210 0.13 -2.6 21,162 + EX31_RS14210 0.13 +0.5 21,163 - EX31_RS14210 0.13 +1.1 21,163 - EX31_RS14210 0.13 -0.2 21,163 - EX31_RS14210 0.13 +0.3 21,163 - EX31_RS14210 0.13 -0.2 21,230 - EX31_RS14210 0.15 -1.0 21,283 - EX31_RS14210 0.18 -0.2 21,328 - EX31_RS14210 0.19 +1.4 21,328 - EX31_RS14210 0.19 -1.0 21,438 + EX31_RS14210 0.24 -3.4 21,439 - EX31_RS14210 0.24 +0.3 21,452 + EX31_RS14210 0.25 -0.1 21,452 + EX31_RS14210 0.25 -0.7 21,452 + EX31_RS14210 0.25 -0.1 21,453 - EX31_RS14210 0.25 +0.3 21,453 - EX31_RS14210 0.25 +0.1 21,453 - EX31_RS14210 0.25 -0.6 21,453 - EX31_RS14210 0.25 +1.0 21,453 - EX31_RS14210 0.25 +0.6 21,453 - EX31_RS14210 0.25 +2.0 21,453 - EX31_RS14210 0.25 +1.0 21,489 - EX31_RS14210 0.26 +3.5 21,490 + EX31_RS14210 0.26 +0.5 21,490 + EX31_RS14210 0.26 -0.9 21,490 + EX31_RS14210 0.26 +0.3 21,490 + EX31_RS14210 0.26 -0.2 21,490 + EX31_RS14210 0.26 +0.2 21,490 + EX31_RS14210 0.26 -0.1 21,490 + EX31_RS14210 0.26 +1.5 21,490 + EX31_RS14210 0.26 +0.4 21,491 - EX31_RS14210 0.26 -0.9 21,491 - EX31_RS14210 0.26 -0.9 21,491 - EX31_RS14210 0.26 +0.0 21,491 - EX31_RS14210 0.26 +0.9 21,491 - EX31_RS14210 0.26 -0.1 21,491 - EX31_RS14210 0.26 +1.2 21,491 - EX31_RS14210 0.26 +0.8 21,491 - EX31_RS14210 0.26 -0.5 21,491 - EX31_RS14210 0.26 +0.0 21,538 + EX31_RS14210 0.28 +0.1 21,539 - EX31_RS14210 0.28 -0.1 21,542 + EX31_RS14210 0.28 -0.3 21,542 + EX31_RS14210 0.28 +0.0 21,542 + EX31_RS14210 0.28 +0.1 21,542 + EX31_RS14210 0.28 +0.2 21,542 + EX31_RS14210 0.28 -0.7 21,543 - EX31_RS14210 0.28 +0.3 21,543 - EX31_RS14210 0.28 +0.4 21,595 + EX31_RS14210 0.30 +0.7 21,595 + EX31_RS14210 0.30 +0.9 21,595 + EX31_RS14210 0.30 +1.2 21,596 - EX31_RS14210 0.31 +0.5 21,596 - EX31_RS14210 0.31 +1.1 21,596 - EX31_RS14210 0.31 +0.2
Or see this region's nucleotide sequence