Experiment: Ammonium chloride (N); RCH2 media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS08045 and EX31_RS08050 are separated by 65 nucleotides EX31_RS08050 and EX31_RS08055 are separated by 134 nucleotides
EX31_RS08045: EX31_RS08045 - 2,5-didehydrogluconate reductase DkgA, at 99,815 to 100,639
_RS08045
EX31_RS08050: EX31_RS08050 - cell division protein FtsP, at 100,705 to 102,120
_RS08050
EX31_RS08055: EX31_RS08055 - 1-acylglycerol-3-phosphate O-acyltransferase, at 102,255 to 102,992
_RS08055
Position (kb) 100
101
102
103 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 99.708 kb on + strand at 99.708 kb on + strand at 99.709 kb on - strand at 99.709 kb on - strand at 99.712 kb on + strand at 99.744 kb on + strand at 99.745 kb on - strand at 99.750 kb on + strand at 99.750 kb on + strand at 99.751 kb on - strand at 99.751 kb on - strand at 99.751 kb on - strand at 99.751 kb on - strand at 99.751 kb on - strand at 99.752 kb on - strand at 99.764 kb on - strand at 99.867 kb on + strand at 99.868 kb on - strand at 99.879 kb on + strand at 99.965 kb on + strand, within EX31_RS08045 at 100.003 kb on + strand, within EX31_RS08045 at 100.030 kb on + strand, within EX31_RS08045 at 100.030 kb on + strand, within EX31_RS08045 at 100.031 kb on - strand, within EX31_RS08045 at 100.034 kb on - strand, within EX31_RS08045 at 100.039 kb on + strand, within EX31_RS08045 at 100.039 kb on + strand, within EX31_RS08045 at 100.039 kb on + strand, within EX31_RS08045 at 100.039 kb on + strand, within EX31_RS08045 at 100.039 kb on + strand, within EX31_RS08045 at 100.039 kb on + strand, within EX31_RS08045 at 100.040 kb on - strand, within EX31_RS08045 at 100.040 kb on - strand, within EX31_RS08045 at 100.040 kb on - strand, within EX31_RS08045 at 100.040 kb on - strand, within EX31_RS08045 at 100.040 kb on - strand, within EX31_RS08045 at 100.040 kb on - strand, within EX31_RS08045 at 100.040 kb on - strand, within EX31_RS08045 at 100.062 kb on + strand, within EX31_RS08045 at 100.062 kb on + strand, within EX31_RS08045 at 100.062 kb on + strand, within EX31_RS08045 at 100.112 kb on + strand, within EX31_RS08045 at 100.112 kb on + strand, within EX31_RS08045 at 100.112 kb on + strand, within EX31_RS08045 at 100.113 kb on - strand, within EX31_RS08045 at 100.113 kb on - strand, within EX31_RS08045 at 100.113 kb on - strand, within EX31_RS08045 at 100.124 kb on + strand, within EX31_RS08045 at 100.125 kb on - strand, within EX31_RS08045 at 100.160 kb on + strand, within EX31_RS08045 at 100.160 kb on + strand, within EX31_RS08045 at 100.160 kb on + strand, within EX31_RS08045 at 100.160 kb on + strand, within EX31_RS08045 at 100.160 kb on + strand, within EX31_RS08045 at 100.160 kb on + strand, within EX31_RS08045 at 100.161 kb on - strand, within EX31_RS08045 at 100.229 kb on - strand, within EX31_RS08045 at 100.229 kb on - strand, within EX31_RS08045 at 100.229 kb on - strand, within EX31_RS08045 at 100.248 kb on + strand, within EX31_RS08045 at 100.248 kb on + strand, within EX31_RS08045 at 100.249 kb on - strand, within EX31_RS08045 at 100.249 kb on - strand, within EX31_RS08045 at 100.302 kb on + strand, within EX31_RS08045 at 100.302 kb on + strand, within EX31_RS08045 at 100.302 kb on + strand, within EX31_RS08045 at 100.303 kb on - strand, within EX31_RS08045 at 100.402 kb on - strand, within EX31_RS08045 at 100.439 kb on + strand, within EX31_RS08045 at 100.519 kb on + strand, within EX31_RS08045 at 100.520 kb on - strand, within EX31_RS08045 at 100.520 kb on - strand, within EX31_RS08045 at 100.525 kb on + strand, within EX31_RS08045 at 100.525 kb on + strand, within EX31_RS08045 at 100.525 kb on + strand, within EX31_RS08045 at 100.526 kb on - strand, within EX31_RS08045 at 100.526 kb on - strand, within EX31_RS08045 at 100.526 kb on - strand, within EX31_RS08045 at 100.555 kb on + strand, within EX31_RS08045 at 100.555 kb on + strand, within EX31_RS08045 at 100.597 kb on + strand at 100.598 kb on - strand at 100.637 kb on + strand at 100.659 kb on - strand at 100.688 kb on + strand at 100.688 kb on + strand at 100.688 kb on + strand at 100.689 kb on - strand at 100.689 kb on - strand at 100.689 kb on - strand at 100.689 kb on - strand at 100.692 kb on + strand at 100.692 kb on + strand at 100.693 kb on - strand at 100.693 kb on - strand at 100.693 kb on - strand at 100.706 kb on + strand at 100.706 kb on + strand at 100.706 kb on + strand at 100.706 kb on + strand at 100.706 kb on + strand at 100.706 kb on + strand at 100.706 kb on + strand at 100.706 kb on + strand at 100.706 kb on + strand at 100.707 kb on - strand at 100.707 kb on - strand at 100.707 kb on - strand at 100.707 kb on - strand at 100.707 kb on - strand at 100.707 kb on - strand at 100.707 kb on - strand at 100.707 kb on - strand at 100.707 kb on - strand at 100.707 kb on - strand at 100.707 kb on - strand at 100.707 kb on - strand at 100.728 kb on + strand at 100.728 kb on + strand at 100.729 kb on - strand at 100.729 kb on - strand at 100.796 kb on + strand at 100.814 kb on + strand at 100.814 kb on + strand at 100.814 kb on + strand at 100.823 kb on - strand at 101.268 kb on + strand, within EX31_RS08050 at 101.316 kb on + strand, within EX31_RS08050 at 101.326 kb on - strand, within EX31_RS08050 at 101.459 kb on + strand, within EX31_RS08050 at 101.459 kb on + strand, within EX31_RS08050 at 101.460 kb on - strand, within EX31_RS08050 at 101.460 kb on - strand, within EX31_RS08050 at 101.460 kb on - strand, within EX31_RS08050 at 101.460 kb on - strand, within EX31_RS08050 at 101.480 kb on - strand, within EX31_RS08050 at 101.569 kb on - strand, within EX31_RS08050 at 101.569 kb on - strand, within EX31_RS08050 at 101.591 kb on + strand, within EX31_RS08050 at 101.592 kb on - strand, within EX31_RS08050 at 101.661 kb on - strand, within EX31_RS08050 at 101.699 kb on + strand, within EX31_RS08050 at 101.821 kb on - strand, within EX31_RS08050 at 101.821 kb on - strand, within EX31_RS08050 at 101.849 kb on + strand, within EX31_RS08050 at 101.849 kb on + strand, within EX31_RS08050 at 101.849 kb on + strand, within EX31_RS08050 at 101.853 kb on + strand, within EX31_RS08050 at 101.853 kb on + strand, within EX31_RS08050 at 101.854 kb on - strand, within EX31_RS08050 at 101.900 kb on + strand, within EX31_RS08050 at 101.900 kb on + strand, within EX31_RS08050 at 101.900 kb on + strand, within EX31_RS08050 at 101.900 kb on + strand, within EX31_RS08050 at 101.901 kb on - strand, within EX31_RS08050 at 101.901 kb on - strand, within EX31_RS08050 at 101.901 kb on - strand, within EX31_RS08050 at 101.901 kb on - strand, within EX31_RS08050 at 101.910 kb on + strand, within EX31_RS08050 at 102.020 kb on - strand at 102.020 kb on - strand at 102.020 kb on - strand at 102.052 kb on + strand at 102.052 kb on + strand at 102.053 kb on - strand at 102.053 kb on - strand at 102.053 kb on - strand at 102.120 kb on + strand at 102.120 kb on + strand at 102.120 kb on + strand at 102.121 kb on - strand at 102.121 kb on - strand at 102.121 kb on - strand at 102.121 kb on - strand at 102.144 kb on + strand at 102.145 kb on - strand at 102.152 kb on - strand at 102.152 kb on - strand at 102.197 kb on + strand at 102.197 kb on + strand at 102.197 kb on + strand at 102.197 kb on + strand at 102.198 kb on - strand at 102.198 kb on - strand at 102.198 kb on - strand at 102.198 kb on - strand at 102.198 kb on - strand at 102.198 kb on - strand at 102.232 kb on - strand at 102.245 kb on - strand at 102.245 kb on - strand at 102.245 kb on - strand at 102.246 kb on + strand at 102.250 kb on + strand at 102.251 kb on - strand at 102.251 kb on - strand at 102.251 kb on - strand at 102.254 kb on - strand at 102.254 kb on - strand at 102.256 kb on + strand at 102.257 kb on - strand at 103.004 kb on - strand at 103.004 kb on - strand at 103.010 kb on - strand at 103.010 kb on - strand at 103.010 kb on - strand at 103.063 kb on - strand at 103.063 kb on - strand at 103.063 kb on - strand at 103.099 kb on - strand at 103.099 kb on - strand at 103.105 kb on - strand at 103.105 kb on - strand at 103.105 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N); RCH2 media; shaking remove 99,708 + +0.4 99,708 + -1.3 99,709 - +0.1 99,709 - -0.1 99,712 + +0.9 99,744 + +0.3 99,745 - +0.4 99,750 + -1.0 99,750 + -0.0 99,751 - +0.1 99,751 - +0.2 99,751 - +0.1 99,751 - -0.1 99,751 - -0.4 99,752 - -0.3 99,764 - +0.0 99,867 + +0.8 99,868 - +1.1 99,879 + -0.6 99,965 + EX31_RS08045 0.18 +1.3 100,003 + EX31_RS08045 0.23 +0.6 100,030 + EX31_RS08045 0.26 +0.2 100,030 + EX31_RS08045 0.26 +1.0 100,031 - EX31_RS08045 0.26 -2.2 100,034 - EX31_RS08045 0.27 -0.5 100,039 + EX31_RS08045 0.27 +2.0 100,039 + EX31_RS08045 0.27 +0.6 100,039 + EX31_RS08045 0.27 -1.4 100,039 + EX31_RS08045 0.27 -0.2 100,039 + EX31_RS08045 0.27 -0.7 100,039 + EX31_RS08045 0.27 +1.2 100,040 - EX31_RS08045 0.27 -0.2 100,040 - EX31_RS08045 0.27 +0.7 100,040 - EX31_RS08045 0.27 -0.7 100,040 - EX31_RS08045 0.27 -2.3 100,040 - EX31_RS08045 0.27 +0.2 100,040 - EX31_RS08045 0.27 -2.7 100,040 - EX31_RS08045 0.27 +0.8 100,062 + EX31_RS08045 0.30 +0.3 100,062 + EX31_RS08045 0.30 +0.2 100,062 + EX31_RS08045 0.30 +0.2 100,112 + EX31_RS08045 0.36 +0.2 100,112 + EX31_RS08045 0.36 +0.3 100,112 + EX31_RS08045 0.36 +0.2 100,113 - EX31_RS08045 0.36 +0.7 100,113 - EX31_RS08045 0.36 +0.4 100,113 - EX31_RS08045 0.36 -1.4 100,124 + EX31_RS08045 0.37 -1.1 100,125 - EX31_RS08045 0.38 +0.3 100,160 + EX31_RS08045 0.42 -1.5 100,160 + EX31_RS08045 0.42 +0.2 100,160 + EX31_RS08045 0.42 +0.3 100,160 + EX31_RS08045 0.42 +0.3 100,160 + EX31_RS08045 0.42 -0.6 100,160 + EX31_RS08045 0.42 -0.3 100,161 - EX31_RS08045 0.42 -0.6 100,229 - EX31_RS08045 0.50 +0.9 100,229 - EX31_RS08045 0.50 -1.9 100,229 - EX31_RS08045 0.50 +1.1 100,248 + EX31_RS08045 0.52 +0.7 100,248 + EX31_RS08045 0.52 +0.3 100,249 - EX31_RS08045 0.53 +0.3 100,249 - EX31_RS08045 0.53 +0.4 100,302 + EX31_RS08045 0.59 +1.1 100,302 + EX31_RS08045 0.59 +0.3 100,302 + EX31_RS08045 0.59 -1.6 100,303 - EX31_RS08045 0.59 +0.4 100,402 - EX31_RS08045 0.71 -0.2 100,439 + EX31_RS08045 0.76 +0.8 100,519 + EX31_RS08045 0.85 -0.7 100,520 - EX31_RS08045 0.85 -0.5 100,520 - EX31_RS08045 0.85 -0.4 100,525 + EX31_RS08045 0.86 -0.6 100,525 + EX31_RS08045 0.86 +1.0 100,525 + EX31_RS08045 0.86 +0.6 100,526 - EX31_RS08045 0.86 +0.7 100,526 - EX31_RS08045 0.86 +0.8 100,526 - EX31_RS08045 0.86 +0.8 100,555 + EX31_RS08045 0.90 +0.4 100,555 + EX31_RS08045 0.90 +0.6 100,597 + -0.6 100,598 - +0.7 100,637 + +0.3 100,659 - +0.5 100,688 + +1.4 100,688 + +1.4 100,688 + -0.2 100,689 - +0.2 100,689 - -0.8 100,689 - +0.5 100,689 - -0.4 100,692 + +0.9 100,692 + -0.5 100,693 - -0.3 100,693 - -0.2 100,693 - +0.3 100,706 + +1.2 100,706 + +0.1 100,706 + +0.8 100,706 + +1.1 100,706 + +0.5 100,706 + +0.4 100,706 + +0.9 100,706 + +0.7 100,706 + +0.1 100,707 - +0.4 100,707 - +1.1 100,707 - -0.1 100,707 - +1.5 100,707 - +0.5 100,707 - -0.6 100,707 - +0.9 100,707 - -0.9 100,707 - -1.6 100,707 - +0.9 100,707 - +0.0 100,707 - +1.1 100,728 + +0.1 100,728 + +0.1 100,729 - +1.2 100,729 - +0.1 100,796 + +1.6 100,814 + -0.8 100,814 + +0.1 100,814 + +0.9 100,823 - +0.8 101,268 + EX31_RS08050 0.40 +0.0 101,316 + EX31_RS08050 0.43 +0.8 101,326 - EX31_RS08050 0.44 +0.8 101,459 + EX31_RS08050 0.53 -0.3 101,459 + EX31_RS08050 0.53 +2.0 101,460 - EX31_RS08050 0.53 +1.5 101,460 - EX31_RS08050 0.53 +1.5 101,460 - EX31_RS08050 0.53 -0.9 101,460 - EX31_RS08050 0.53 +0.5 101,480 - EX31_RS08050 0.55 +0.2 101,569 - EX31_RS08050 0.61 +0.6 101,569 - EX31_RS08050 0.61 +0.1 101,591 + EX31_RS08050 0.63 +0.6 101,592 - EX31_RS08050 0.63 +0.4 101,661 - EX31_RS08050 0.68 +1.0 101,699 + EX31_RS08050 0.70 +0.1 101,821 - EX31_RS08050 0.79 +0.6 101,821 - EX31_RS08050 0.79 +0.1 101,849 + EX31_RS08050 0.81 +1.1 101,849 + EX31_RS08050 0.81 +0.3 101,849 + EX31_RS08050 0.81 +2.4 101,853 + EX31_RS08050 0.81 +1.2 101,853 + EX31_RS08050 0.81 +0.2 101,854 - EX31_RS08050 0.81 +0.6 101,900 + EX31_RS08050 0.84 +3.1 101,900 + EX31_RS08050 0.84 +1.1 101,900 + EX31_RS08050 0.84 +1.5 101,900 + EX31_RS08050 0.84 +1.0 101,901 - EX31_RS08050 0.84 +0.4 101,901 - EX31_RS08050 0.84 -1.6 101,901 - EX31_RS08050 0.84 +0.6 101,901 - EX31_RS08050 0.84 +1.0 101,910 + EX31_RS08050 0.85 +0.9 102,020 - +4.0 102,020 - +1.5 102,020 - +1.1 102,052 + +1.6 102,052 + +0.4 102,053 - +1.8 102,053 - +0.7 102,053 - -0.1 102,120 + +0.6 102,120 + +0.4 102,120 + +0.4 102,121 - +0.6 102,121 - -1.3 102,121 - +0.4 102,121 - +1.1 102,144 + +1.5 102,145 - +0.9 102,152 - +0.6 102,152 - +0.2 102,197 + +0.5 102,197 + -0.9 102,197 + -0.0 102,197 + +2.6 102,198 - -0.0 102,198 - +0.5 102,198 - +0.5 102,198 - +0.6 102,198 - +0.4 102,198 - +0.1 102,232 - -0.5 102,245 - +0.2 102,245 - +1.0 102,245 - -1.4 102,246 + -0.3 102,250 + -0.7 102,251 - -0.6 102,251 - -1.6 102,251 - -0.4 102,254 - +0.6 102,254 - +0.1 102,256 + -0.8 102,257 - +0.2 103,004 - -0.8 103,004 - +0.1 103,010 - -1.5 103,010 - +0.5 103,010 - -2.2 103,063 - +0.0 103,063 - +0.8 103,063 - -0.6 103,099 - -0.5 103,099 - +0.3 103,105 - -0.4 103,105 - -0.2 103,105 - +0.3
Or see this region's nucleotide sequence