Experiment: Ammonium chloride (N); RCH2 media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS06475 and EX31_RS06480 overlap by 4 nucleotides EX31_RS06480 and EX31_RS06485 are separated by 56 nucleotides EX31_RS06485 and EX31_RS06490 are separated by 121 nucleotides
EX31_RS06475: EX31_RS06475 - YdbH family protein, at 64,912 to 67,638
_RS06475
EX31_RS06480: EX31_RS06480 - YnbE family lipoprotein, at 67,635 to 67,829
_RS06480
EX31_RS06485: EX31_RS06485 - YdbL family protein, at 67,886 to 68,191
_RS06485
EX31_RS06490: EX31_RS06490 - FMN-dependent NADH-azoreductase, at 68,313 to 68,930
_RS06490
Position (kb) 67
68
69 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 67.037 kb on + strand, within EX31_RS06475 at 67.038 kb on - strand, within EX31_RS06475 at 67.038 kb on - strand, within EX31_RS06475 at 67.071 kb on + strand, within EX31_RS06475 at 67.311 kb on + strand, within EX31_RS06475 at 67.311 kb on + strand, within EX31_RS06475 at 67.311 kb on + strand, within EX31_RS06475 at 67.311 kb on + strand, within EX31_RS06475 at 67.312 kb on - strand, within EX31_RS06475 at 67.374 kb on + strand at 67.421 kb on - strand at 67.474 kb on + strand at 67.491 kb on + strand at 67.491 kb on + strand at 67.492 kb on - strand at 67.496 kb on - strand at 67.496 kb on - strand at 68.153 kb on + strand, within EX31_RS06485 at 68.153 kb on + strand, within EX31_RS06485 at 68.153 kb on + strand, within EX31_RS06485 at 68.173 kb on + strand at 68.174 kb on - strand at 68.189 kb on + strand at 68.189 kb on + strand at 68.237 kb on - strand at 68.279 kb on - strand at 68.279 kb on - strand at 68.310 kb on + strand at 68.310 kb on + strand at 68.311 kb on - strand at 68.311 kb on - strand at 68.311 kb on - strand at 68.314 kb on + strand at 68.314 kb on + strand at 68.315 kb on - strand at 68.315 kb on - strand at 68.395 kb on + strand, within EX31_RS06490 at 68.403 kb on - strand, within EX31_RS06490 at 68.425 kb on - strand, within EX31_RS06490 at 68.464 kb on + strand, within EX31_RS06490 at 68.465 kb on - strand, within EX31_RS06490 at 68.465 kb on - strand, within EX31_RS06490 at 68.465 kb on - strand, within EX31_RS06490 at 68.465 kb on - strand, within EX31_RS06490 at 68.473 kb on - strand, within EX31_RS06490 at 68.608 kb on + strand, within EX31_RS06490 at 68.641 kb on + strand, within EX31_RS06490 at 68.641 kb on + strand, within EX31_RS06490 at 68.641 kb on + strand, within EX31_RS06490 at 68.642 kb on - strand, within EX31_RS06490 at 68.699 kb on + strand, within EX31_RS06490 at 68.778 kb on + strand, within EX31_RS06490 at 68.778 kb on + strand, within EX31_RS06490 at 68.778 kb on + strand, within EX31_RS06490 at 68.779 kb on - strand, within EX31_RS06490 at 68.779 kb on - strand, within EX31_RS06490 at 68.779 kb on - strand, within EX31_RS06490 at 68.779 kb on - strand, within EX31_RS06490 at 68.781 kb on + strand, within EX31_RS06490 at 68.782 kb on - strand, within EX31_RS06490 at 68.782 kb on - strand, within EX31_RS06490 at 68.845 kb on + strand, within EX31_RS06490 at 68.920 kb on - strand at 68.944 kb on - strand at 68.954 kb on + strand at 68.954 kb on + strand at 68.955 kb on - strand at 68.955 kb on - strand at 68.958 kb on + strand at 68.958 kb on + strand at 68.959 kb on - strand at 68.974 kb on - strand at 68.975 kb on + strand at 68.976 kb on - strand at 69.003 kb on - strand at 69.003 kb on - strand at 69.003 kb on - strand at 69.003 kb on - strand at 69.003 kb on - strand at 69.042 kb on + strand at 69.043 kb on - strand at 69.043 kb on - strand at 69.114 kb on + strand at 69.124 kb on + strand at 69.129 kb on + strand at 69.130 kb on - strand at 69.130 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N); RCH2 media; shaking remove 67,037 + EX31_RS06475 0.78 +0.3 67,038 - EX31_RS06475 0.78 +1.3 67,038 - EX31_RS06475 0.78 -2.6 67,071 + EX31_RS06475 0.79 +1.4 67,311 + EX31_RS06475 0.88 -0.2 67,311 + EX31_RS06475 0.88 +1.5 67,311 + EX31_RS06475 0.88 +0.3 67,311 + EX31_RS06475 0.88 -0.8 67,312 - EX31_RS06475 0.88 +0.1 67,374 + -0.5 67,421 - -1.3 67,474 + -0.7 67,491 + -0.2 67,491 + -0.4 67,492 - -0.9 67,496 - -0.5 67,496 - -1.4 68,153 + EX31_RS06485 0.87 +0.2 68,153 + EX31_RS06485 0.87 -0.9 68,153 + EX31_RS06485 0.87 -0.3 68,173 + +1.9 68,174 - -1.1 68,189 + +0.8 68,189 + -1.1 68,237 - -0.6 68,279 - -0.6 68,279 - +2.1 68,310 + +0.8 68,310 + +0.1 68,311 - -0.3 68,311 - +0.0 68,311 - +0.3 68,314 + -2.1 68,314 + +2.0 68,315 - -1.5 68,315 - -0.6 68,395 + EX31_RS06490 0.13 +1.1 68,403 - EX31_RS06490 0.15 +2.8 68,425 - EX31_RS06490 0.18 +1.5 68,464 + EX31_RS06490 0.24 +0.3 68,465 - EX31_RS06490 0.25 -1.3 68,465 - EX31_RS06490 0.25 +0.6 68,465 - EX31_RS06490 0.25 +0.2 68,465 - EX31_RS06490 0.25 -1.1 68,473 - EX31_RS06490 0.26 -0.6 68,608 + EX31_RS06490 0.48 -0.3 68,641 + EX31_RS06490 0.53 +1.3 68,641 + EX31_RS06490 0.53 -0.1 68,641 + EX31_RS06490 0.53 +0.0 68,642 - EX31_RS06490 0.53 +1.5 68,699 + EX31_RS06490 0.62 +1.4 68,778 + EX31_RS06490 0.75 -0.2 68,778 + EX31_RS06490 0.75 +0.2 68,778 + EX31_RS06490 0.75 -0.4 68,779 - EX31_RS06490 0.75 -0.9 68,779 - EX31_RS06490 0.75 +1.7 68,779 - EX31_RS06490 0.75 -1.5 68,779 - EX31_RS06490 0.75 -0.2 68,781 + EX31_RS06490 0.76 +0.1 68,782 - EX31_RS06490 0.76 +0.3 68,782 - EX31_RS06490 0.76 -0.1 68,845 + EX31_RS06490 0.86 +1.6 68,920 - +1.5 68,944 - -1.8 68,954 + -0.1 68,954 + -0.0 68,955 - -0.7 68,955 - -0.6 68,958 + +1.5 68,958 + -0.5 68,959 - +2.1 68,974 - +3.1 68,975 + +0.5 68,976 - +1.1 69,003 - +0.9 69,003 - -0.5 69,003 - +1.4 69,003 - +1.3 69,003 - +0.5 69,042 + -2.3 69,043 - +0.1 69,043 - -1.1 69,114 + -0.1 69,124 + -0.8 69,129 + +0.5 69,130 - +0.9 69,130 - -1.2
Or see this region's nucleotide sequence