Experiment: Ammonium chloride (N); RCH2 media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS03260 and EX31_RS03265 overlap by 4 nucleotides EX31_RS03265 and EX31_RS03270 are separated by 5 nucleotides EX31_RS03270 and EX31_RS03275 overlap by 4 nucleotides EX31_RS03275 and EX31_RS03280 are separated by 158 nucleotides
EX31_RS03260: EX31_RS03260 - polynucleotide adenylyltransferase PcnB, at 142,836 to 144,542
_RS03260
EX31_RS03265: EX31_RS03265 - 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine diphosphokinase, at 144,539 to 145,018
_RS03265
EX31_RS03270: EX31_RS03270 - two-component system sensor histidine kinase QseC, at 145,024 to 146,409
_RS03270
EX31_RS03275: EX31_RS03275 - response regulator, at 146,406 to 147,092
_RS03275
EX31_RS03280: EX31_RS03280 - 3-methyl-2-oxobutanoate hydroxymethyltransferase, at 147,251 to 148,045
_RS03280
Position (kb) 145
146
147 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 144.098 kb on + strand, within EX31_RS03260 at 144.098 kb on + strand, within EX31_RS03260 at 144.098 kb on + strand, within EX31_RS03260 at 144.159 kb on + strand, within EX31_RS03260 at 144.159 kb on + strand, within EX31_RS03260 at 144.159 kb on + strand, within EX31_RS03260 at 144.159 kb on + strand, within EX31_RS03260 at 144.162 kb on + strand, within EX31_RS03260 at 144.233 kb on + strand, within EX31_RS03260 at 144.233 kb on + strand, within EX31_RS03260 at 144.233 kb on + strand, within EX31_RS03260 at 144.233 kb on + strand, within EX31_RS03260 at 144.233 kb on + strand, within EX31_RS03260 at 144.233 kb on + strand, within EX31_RS03260 at 144.233 kb on + strand, within EX31_RS03260 at 144.236 kb on + strand, within EX31_RS03260 at 144.247 kb on + strand, within EX31_RS03260 at 144.247 kb on + strand, within EX31_RS03260 at 144.247 kb on + strand, within EX31_RS03260 at 144.247 kb on + strand, within EX31_RS03260 at 144.254 kb on + strand, within EX31_RS03260 at 144.323 kb on + strand, within EX31_RS03260 at 144.323 kb on + strand, within EX31_RS03260 at 144.323 kb on + strand, within EX31_RS03260 at 144.323 kb on + strand, within EX31_RS03260 at 144.323 kb on + strand, within EX31_RS03260 at 144.323 kb on + strand, within EX31_RS03260 at 144.339 kb on + strand, within EX31_RS03260 at 144.346 kb on + strand, within EX31_RS03260 at 144.352 kb on + strand, within EX31_RS03260 at 144.352 kb on + strand, within EX31_RS03260 at 144.475 kb on + strand at 144.475 kb on + strand at 145.016 kb on + strand at 145.016 kb on + strand at 145.017 kb on - strand at 145.070 kb on + strand at 145.090 kb on + strand at 145.090 kb on + strand at 145.090 kb on + strand at 145.090 kb on + strand at 145.090 kb on + strand at 145.090 kb on + strand at 145.091 kb on - strand at 145.108 kb on + strand at 145.108 kb on + strand at 145.109 kb on - strand at 145.109 kb on - strand at 145.109 kb on - strand at 145.160 kb on + strand at 145.444 kb on - strand, within EX31_RS03270 at 145.609 kb on + strand, within EX31_RS03270 at 145.609 kb on + strand, within EX31_RS03270 at 145.609 kb on + strand, within EX31_RS03270 at 145.609 kb on + strand, within EX31_RS03270 at 145.609 kb on + strand, within EX31_RS03270 at 145.610 kb on - strand, within EX31_RS03270 at 145.610 kb on - strand, within EX31_RS03270 at 145.610 kb on - strand, within EX31_RS03270 at 145.610 kb on - strand, within EX31_RS03270 at 145.653 kb on + strand, within EX31_RS03270 at 145.654 kb on - strand, within EX31_RS03270 at 145.687 kb on - strand, within EX31_RS03270 at 145.912 kb on - strand, within EX31_RS03270 at 145.984 kb on - strand, within EX31_RS03270 at 146.020 kb on + strand, within EX31_RS03270 at 146.022 kb on + strand, within EX31_RS03270 at 146.022 kb on + strand, within EX31_RS03270 at 146.022 kb on + strand, within EX31_RS03270 at 146.212 kb on + strand, within EX31_RS03270 at 146.274 kb on + strand at 146.274 kb on + strand at 146.274 kb on + strand at 146.275 kb on - strand at 146.275 kb on - strand at 146.275 kb on - strand at 146.352 kb on + strand at 146.353 kb on - strand at 146.434 kb on - strand at 146.514 kb on - strand, within EX31_RS03275 at 146.557 kb on + strand, within EX31_RS03275 at 146.558 kb on - strand, within EX31_RS03275 at 146.573 kb on + strand, within EX31_RS03275 at 146.574 kb on - strand, within EX31_RS03275 at 146.792 kb on + strand, within EX31_RS03275 at 146.792 kb on + strand, within EX31_RS03275 at 146.800 kb on + strand, within EX31_RS03275 at 146.800 kb on + strand, within EX31_RS03275 at 146.800 kb on + strand, within EX31_RS03275 at 146.801 kb on - strand, within EX31_RS03275 at 146.801 kb on - strand, within EX31_RS03275 at 146.801 kb on - strand, within EX31_RS03275 at 146.864 kb on + strand, within EX31_RS03275 at 146.915 kb on + strand, within EX31_RS03275 at 146.915 kb on + strand, within EX31_RS03275 at 146.915 kb on + strand, within EX31_RS03275 at 146.916 kb on - strand, within EX31_RS03275 at 146.916 kb on - strand, within EX31_RS03275 at 146.937 kb on + strand, within EX31_RS03275 at 146.937 kb on + strand, within EX31_RS03275 at 146.938 kb on - strand, within EX31_RS03275 at 146.960 kb on - strand, within EX31_RS03275 at 147.017 kb on + strand, within EX31_RS03275 at 147.081 kb on + strand at 147.114 kb on + strand at 147.115 kb on - strand at 147.116 kb on + strand at 147.116 kb on + strand at 147.116 kb on + strand at 147.116 kb on + strand at 147.117 kb on - strand at 147.117 kb on - strand at 147.117 kb on - strand at 147.131 kb on - strand at 147.141 kb on + strand at 147.141 kb on + strand at 147.141 kb on + strand at 147.142 kb on - strand at 147.142 kb on - strand at 147.142 kb on - strand at 147.195 kb on + strand at 147.195 kb on + strand at 147.195 kb on + strand at 147.195 kb on + strand at 147.195 kb on + strand at 147.195 kb on + strand at 147.195 kb on + strand at 147.195 kb on + strand at 147.195 kb on + strand at 147.195 kb on + strand at 147.195 kb on + strand at 147.196 kb on - strand at 147.196 kb on - strand at 147.196 kb on - strand at 147.196 kb on - strand at 147.196 kb on - strand at 147.196 kb on - strand at 147.200 kb on + strand at 147.200 kb on + strand at 147.200 kb on + strand at 147.200 kb on + strand at 147.201 kb on - strand at 147.201 kb on - strand at 147.201 kb on - strand at 147.201 kb on - strand at 147.201 kb on - strand at 147.201 kb on - strand at 147.216 kb on + strand at 147.216 kb on + strand at 147.216 kb on + strand at 147.217 kb on - strand at 147.217 kb on - strand at 147.217 kb on - strand at 147.217 kb on - strand at 147.217 kb on - strand at 147.232 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N); RCH2 media; shaking remove 144,098 + EX31_RS03260 0.74 -0.2 144,098 + EX31_RS03260 0.74 +0.6 144,098 + EX31_RS03260 0.74 -0.1 144,159 + EX31_RS03260 0.78 -0.5 144,159 + EX31_RS03260 0.78 -2.4 144,159 + EX31_RS03260 0.78 -0.1 144,159 + EX31_RS03260 0.78 -0.3 144,162 + EX31_RS03260 0.78 -1.0 144,233 + EX31_RS03260 0.82 -0.5 144,233 + EX31_RS03260 0.82 +1.2 144,233 + EX31_RS03260 0.82 -4.0 144,233 + EX31_RS03260 0.82 -3.1 144,233 + EX31_RS03260 0.82 +0.8 144,233 + EX31_RS03260 0.82 +0.2 144,233 + EX31_RS03260 0.82 -0.0 144,236 + EX31_RS03260 0.82 -4.3 144,247 + EX31_RS03260 0.83 -1.6 144,247 + EX31_RS03260 0.83 -0.3 144,247 + EX31_RS03260 0.83 -0.1 144,247 + EX31_RS03260 0.83 +1.1 144,254 + EX31_RS03260 0.83 -1.9 144,323 + EX31_RS03260 0.87 -0.9 144,323 + EX31_RS03260 0.87 +0.4 144,323 + EX31_RS03260 0.87 -2.5 144,323 + EX31_RS03260 0.87 -0.1 144,323 + EX31_RS03260 0.87 +1.5 144,323 + EX31_RS03260 0.87 +0.3 144,339 + EX31_RS03260 0.88 -1.0 144,346 + EX31_RS03260 0.88 -0.0 144,352 + EX31_RS03260 0.89 +0.5 144,352 + EX31_RS03260 0.89 -0.2 144,475 + -1.7 144,475 + +1.0 145,016 + +2.1 145,016 + -0.7 145,017 - +0.8 145,070 + +0.4 145,090 + -0.2 145,090 + +1.1 145,090 + +1.5 145,090 + +0.5 145,090 + +0.3 145,090 + +0.2 145,091 - -0.7 145,108 + +0.5 145,108 + +0.5 145,109 - -0.5 145,109 - -0.3 145,109 - +0.4 145,160 + -0.5 145,444 - EX31_RS03270 0.30 -1.7 145,609 + EX31_RS03270 0.42 -0.4 145,609 + EX31_RS03270 0.42 -0.9 145,609 + EX31_RS03270 0.42 +2.1 145,609 + EX31_RS03270 0.42 +1.8 145,609 + EX31_RS03270 0.42 -0.4 145,610 - EX31_RS03270 0.42 -0.3 145,610 - EX31_RS03270 0.42 -0.2 145,610 - EX31_RS03270 0.42 +0.7 145,610 - EX31_RS03270 0.42 +0.8 145,653 + EX31_RS03270 0.45 +0.5 145,654 - EX31_RS03270 0.45 +1.0 145,687 - EX31_RS03270 0.48 -0.2 145,912 - EX31_RS03270 0.64 -0.9 145,984 - EX31_RS03270 0.69 +2.5 146,020 + EX31_RS03270 0.72 -1.2 146,022 + EX31_RS03270 0.72 +0.8 146,022 + EX31_RS03270 0.72 +1.0 146,022 + EX31_RS03270 0.72 -1.4 146,212 + EX31_RS03270 0.86 -0.3 146,274 + +0.1 146,274 + +3.1 146,274 + -0.3 146,275 - +1.6 146,275 - +0.4 146,275 - +1.2 146,352 + +0.9 146,353 - -0.3 146,434 - -0.4 146,514 - EX31_RS03275 0.16 -1.5 146,557 + EX31_RS03275 0.22 +0.8 146,558 - EX31_RS03275 0.22 +0.2 146,573 + EX31_RS03275 0.24 +0.6 146,574 - EX31_RS03275 0.24 +2.4 146,792 + EX31_RS03275 0.56 +0.8 146,792 + EX31_RS03275 0.56 +0.2 146,800 + EX31_RS03275 0.57 -0.4 146,800 + EX31_RS03275 0.57 -0.9 146,800 + EX31_RS03275 0.57 +0.5 146,801 - EX31_RS03275 0.57 -0.1 146,801 - EX31_RS03275 0.57 +0.4 146,801 - EX31_RS03275 0.57 +1.1 146,864 + EX31_RS03275 0.67 +1.4 146,915 + EX31_RS03275 0.74 +2.2 146,915 + EX31_RS03275 0.74 +2.3 146,915 + EX31_RS03275 0.74 -0.5 146,916 - EX31_RS03275 0.74 +0.2 146,916 - EX31_RS03275 0.74 +0.8 146,937 + EX31_RS03275 0.77 +0.3 146,937 + EX31_RS03275 0.77 -0.1 146,938 - EX31_RS03275 0.77 +0.4 146,960 - EX31_RS03275 0.81 -0.7 147,017 + EX31_RS03275 0.89 +0.0 147,081 + +0.3 147,114 + +0.4 147,115 - -0.6 147,116 + +0.5 147,116 + +0.0 147,116 + +0.4 147,116 + +0.6 147,117 - -0.4 147,117 - +0.1 147,117 - +0.8 147,131 - +0.1 147,141 + +0.7 147,141 + +0.4 147,141 + +0.4 147,142 - -0.2 147,142 - -0.7 147,142 - +0.5 147,195 + +1.3 147,195 + +0.5 147,195 + -0.8 147,195 + +0.6 147,195 + +0.1 147,195 + -2.1 147,195 + -0.5 147,195 + -1.1 147,195 + +0.4 147,195 + +0.2 147,195 + +0.9 147,196 - -0.4 147,196 - -0.1 147,196 - -0.0 147,196 - +0.5 147,196 - +0.1 147,196 - +1.2 147,200 + -1.1 147,200 + -0.6 147,200 + +0.5 147,200 + +0.4 147,201 - -0.4 147,201 - +0.2 147,201 - +0.1 147,201 - -1.0 147,201 - +1.0 147,201 - +0.1 147,216 + +1.6 147,216 + +0.6 147,216 + -0.3 147,217 - +0.2 147,217 - +0.2 147,217 - +0.6 147,217 - -1.0 147,217 - +0.5 147,232 + +0.1
Or see this region's nucleotide sequence