Experiment: Ammonium chloride (N); RCH2 media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS01400 and EX31_RS01405 are separated by 243 nucleotides EX31_RS01405 and EX31_RS01410 are separated by 209 nucleotides EX31_RS01410 and EX31_RS01415 are separated by 4 nucleotides
EX31_RS01400: EX31_RS01400 - dihydrodipicolinate synthase family protein, at 312,063 to 312,953
_RS01400
EX31_RS01405: EX31_RS01405 - hypothetical protein, at 313,197 to 313,604
_RS01405
EX31_RS01410: EX31_RS01410 - hypothetical protein, at 313,814 to 315,163
_RS01410
EX31_RS01415: EX31_RS01415 - virulence factor SrfB, at 315,168 to 318,140
_RS01415
Position (kb)
313
314
315
316 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 312.920 kb on + strand at 312.941 kb on - strand at 312.981 kb on + strand at 312.981 kb on + strand at 313.003 kb on + strand at 313.115 kb on + strand at 313.116 kb on - strand at 313.196 kb on - strand at 313.204 kb on - strand at 313.256 kb on - strand, within EX31_RS01405 at 313.282 kb on - strand, within EX31_RS01405 at 313.296 kb on + strand, within EX31_RS01405 at 313.296 kb on + strand, within EX31_RS01405 at 313.334 kb on + strand, within EX31_RS01405 at 313.371 kb on - strand, within EX31_RS01405 at 313.371 kb on - strand, within EX31_RS01405 at 313.373 kb on + strand, within EX31_RS01405 at 313.373 kb on + strand, within EX31_RS01405 at 313.374 kb on - strand, within EX31_RS01405 at 313.433 kb on - strand, within EX31_RS01405 at 313.445 kb on + strand, within EX31_RS01405 at 313.545 kb on + strand, within EX31_RS01405 at 313.546 kb on - strand, within EX31_RS01405 at 313.546 kb on - strand, within EX31_RS01405 at 313.571 kb on - strand at 313.710 kb on + strand at 313.733 kb on - strand at 313.798 kb on + strand at 313.798 kb on + strand at 313.799 kb on - strand at 313.799 kb on - strand at 313.799 kb on - strand at 313.799 kb on - strand at 313.799 kb on - strand at 313.799 kb on - strand at 313.799 kb on - strand at 313.799 kb on - strand at 313.917 kb on + strand at 313.970 kb on - strand, within EX31_RS01410 at 314.198 kb on + strand, within EX31_RS01410 at 314.198 kb on + strand, within EX31_RS01410 at 314.198 kb on + strand, within EX31_RS01410 at 314.198 kb on + strand, within EX31_RS01410 at 314.199 kb on - strand, within EX31_RS01410 at 314.199 kb on - strand, within EX31_RS01410 at 314.199 kb on - strand, within EX31_RS01410 at 314.199 kb on - strand, within EX31_RS01410 at 314.199 kb on - strand, within EX31_RS01410 at 314.199 kb on - strand, within EX31_RS01410 at 314.290 kb on + strand, within EX31_RS01410 at 314.290 kb on + strand, within EX31_RS01410 at 314.291 kb on - strand, within EX31_RS01410 at 314.532 kb on + strand, within EX31_RS01410 at 314.532 kb on + strand, within EX31_RS01410 at 314.532 kb on + strand, within EX31_RS01410 at 314.532 kb on + strand, within EX31_RS01410 at 314.534 kb on + strand, within EX31_RS01410 at 314.535 kb on - strand, within EX31_RS01410 at 314.761 kb on - strand, within EX31_RS01410 at 314.790 kb on + strand, within EX31_RS01410 at 314.842 kb on + strand, within EX31_RS01410 at 314.842 kb on + strand, within EX31_RS01410 at 314.885 kb on - strand, within EX31_RS01410 at 315.050 kb on + strand at 315.073 kb on + strand at 315.146 kb on - strand at 315.146 kb on - strand at 315.161 kb on + strand at 315.192 kb on - strand at 315.204 kb on - strand at 315.324 kb on + strand at 315.325 kb on - strand at 315.590 kb on - strand, within EX31_RS01415 at 315.724 kb on + strand, within EX31_RS01415 at 315.724 kb on + strand, within EX31_RS01415 at 315.725 kb on - strand, within EX31_RS01415 at 315.733 kb on + strand, within EX31_RS01415 at 315.734 kb on - strand, within EX31_RS01415 at 315.779 kb on + strand, within EX31_RS01415 at 315.780 kb on - strand, within EX31_RS01415 at 315.782 kb on + strand, within EX31_RS01415 at 315.782 kb on + strand, within EX31_RS01415 at 315.782 kb on + strand, within EX31_RS01415 at 315.783 kb on - strand, within EX31_RS01415 at 315.820 kb on - strand, within EX31_RS01415 at 315.820 kb on - strand, within EX31_RS01415 at 315.820 kb on - strand, within EX31_RS01415 at 315.875 kb on + strand, within EX31_RS01415 at 315.932 kb on + strand, within EX31_RS01415 at 315.932 kb on + strand, within EX31_RS01415 at 316.041 kb on + strand, within EX31_RS01415 at 316.134 kb on - strand, within EX31_RS01415
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N); RCH2 media; shaking remove 312,920 + -0.7 312,941 - +0.5 312,981 + +0.8 312,981 + +1.3 313,003 + +0.4 313,115 + -0.1 313,116 - -0.4 313,196 - +0.7 313,204 - +0.9 313,256 - EX31_RS01405 0.14 +1.4 313,282 - EX31_RS01405 0.21 +0.3 313,296 + EX31_RS01405 0.24 +1.8 313,296 + EX31_RS01405 0.24 +1.9 313,334 + EX31_RS01405 0.34 -0.8 313,371 - EX31_RS01405 0.43 +0.5 313,371 - EX31_RS01405 0.43 -0.1 313,373 + EX31_RS01405 0.43 +1.4 313,373 + EX31_RS01405 0.43 +0.8 313,374 - EX31_RS01405 0.43 -0.5 313,433 - EX31_RS01405 0.58 -0.3 313,445 + EX31_RS01405 0.61 -0.6 313,545 + EX31_RS01405 0.85 +1.2 313,546 - EX31_RS01405 0.86 -0.5 313,546 - EX31_RS01405 0.86 +0.6 313,571 - +0.3 313,710 + -0.9 313,733 - -1.0 313,798 + +0.2 313,798 + +0.2 313,799 - -0.5 313,799 - -0.2 313,799 - -0.2 313,799 - -0.2 313,799 - +0.6 313,799 - +1.1 313,799 - -2.4 313,799 - -2.3 313,917 + -0.1 313,970 - EX31_RS01410 0.12 +0.6 314,198 + EX31_RS01410 0.28 -0.7 314,198 + EX31_RS01410 0.28 +0.9 314,198 + EX31_RS01410 0.28 +1.5 314,198 + EX31_RS01410 0.28 -0.3 314,199 - EX31_RS01410 0.29 +0.7 314,199 - EX31_RS01410 0.29 +0.3 314,199 - EX31_RS01410 0.29 -0.5 314,199 - EX31_RS01410 0.29 +0.8 314,199 - EX31_RS01410 0.29 +1.3 314,199 - EX31_RS01410 0.29 +1.1 314,290 + EX31_RS01410 0.35 -0.6 314,290 + EX31_RS01410 0.35 -0.0 314,291 - EX31_RS01410 0.35 -0.2 314,532 + EX31_RS01410 0.53 -0.4 314,532 + EX31_RS01410 0.53 -0.5 314,532 + EX31_RS01410 0.53 +2.0 314,532 + EX31_RS01410 0.53 +1.1 314,534 + EX31_RS01410 0.53 -1.8 314,535 - EX31_RS01410 0.53 -0.5 314,761 - EX31_RS01410 0.70 -0.2 314,790 + EX31_RS01410 0.72 -1.9 314,842 + EX31_RS01410 0.76 -0.1 314,842 + EX31_RS01410 0.76 +0.5 314,885 - EX31_RS01410 0.79 +0.8 315,050 + +1.5 315,073 + +0.6 315,146 - +1.3 315,146 - +1.3 315,161 + +0.7 315,192 - +0.8 315,204 - +0.8 315,324 + -0.3 315,325 - -0.5 315,590 - EX31_RS01415 0.14 +0.8 315,724 + EX31_RS01415 0.19 -1.2 315,724 + EX31_RS01415 0.19 -0.2 315,725 - EX31_RS01415 0.19 +0.7 315,733 + EX31_RS01415 0.19 -0.2 315,734 - EX31_RS01415 0.19 -1.0 315,779 + EX31_RS01415 0.21 +0.2 315,780 - EX31_RS01415 0.21 -0.2 315,782 + EX31_RS01415 0.21 +1.1 315,782 + EX31_RS01415 0.21 +0.2 315,782 + EX31_RS01415 0.21 +1.1 315,783 - EX31_RS01415 0.21 +1.6 315,820 - EX31_RS01415 0.22 -2.3 315,820 - EX31_RS01415 0.22 -0.1 315,820 - EX31_RS01415 0.22 +0.1 315,875 + EX31_RS01415 0.24 -0.2 315,932 + EX31_RS01415 0.26 -0.0 315,932 + EX31_RS01415 0.26 +1.4 316,041 + EX31_RS01415 0.29 +1.6 316,134 - EX31_RS01415 0.32 +1.8
Or see this region's nucleotide sequence