Experiment: R2A with Chloride 300 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS21095 and CA265_RS21100 are separated by 125 nucleotides CA265_RS21100 and CA265_RS21105 are separated by 61 nucleotides CA265_RS21105 and CA265_RS21110 are separated by 78 nucleotides
CA265_RS21095: CA265_RS21095 - 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase, at 5,197,835 to 5,200,168
_RS21095
CA265_RS21100: CA265_RS21100 - acyl-CoA thioesterase, at 5,200,294 to 5,200,680
_RS21100
CA265_RS21105: CA265_RS21105 - 16S rRNA (cytosine(1402)-N(4))-methyltransferase, at 5,200,742 to 5,201,407
_RS21105
CA265_RS21110: CA265_RS21110 - phosphatidate cytidylyltransferase, at 5,201,486 to 5,202,295
_RS21110
Position (kb)
5200
5201 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 5199.334 kb on + strand, within CA265_RS21095 at 5199.407 kb on + strand, within CA265_RS21095 at 5199.407 kb on + strand, within CA265_RS21095 at 5199.408 kb on - strand, within CA265_RS21095 at 5199.474 kb on + strand, within CA265_RS21095 at 5199.475 kb on - strand, within CA265_RS21095 at 5199.512 kb on + strand at 5199.529 kb on - strand, within CA265_RS21095 at 5199.549 kb on + strand, within CA265_RS21095 at 5199.550 kb on - strand, within CA265_RS21095 at 5199.550 kb on - strand, within CA265_RS21095 at 5199.651 kb on + strand, within CA265_RS21095 at 5199.651 kb on + strand, within CA265_RS21095 at 5199.722 kb on + strand, within CA265_RS21095 at 5199.723 kb on - strand, within CA265_RS21095 at 5199.739 kb on + strand, within CA265_RS21095 at 5199.739 kb on + strand, within CA265_RS21095 at 5199.803 kb on + strand, within CA265_RS21095 at 5199.913 kb on + strand, within CA265_RS21095 at 5199.945 kb on - strand at 5200.093 kb on - strand at 5200.127 kb on - strand at 5200.167 kb on - strand at 5200.210 kb on + strand at 5200.284 kb on + strand at 5200.285 kb on - strand at 5200.357 kb on - strand, within CA265_RS21100 at 5200.453 kb on - strand, within CA265_RS21100 at 5200.464 kb on - strand, within CA265_RS21100 at 5200.509 kb on + strand, within CA265_RS21100 at 5200.509 kb on + strand, within CA265_RS21100 at 5200.510 kb on - strand, within CA265_RS21100 at 5200.510 kb on - strand, within CA265_RS21100 at 5200.533 kb on - strand, within CA265_RS21100 at 5200.548 kb on + strand, within CA265_RS21100 at 5200.577 kb on - strand, within CA265_RS21100 at 5200.591 kb on + strand, within CA265_RS21100 at 5200.777 kb on + strand at 5200.847 kb on - strand, within CA265_RS21105 at 5200.948 kb on - strand, within CA265_RS21105 at 5200.948 kb on - strand, within CA265_RS21105 at 5201.077 kb on + strand, within CA265_RS21105 at 5201.077 kb on + strand, within CA265_RS21105 at 5201.078 kb on - strand, within CA265_RS21105 at 5201.125 kb on + strand, within CA265_RS21105 at 5201.126 kb on - strand, within CA265_RS21105 at 5201.133 kb on - strand, within CA265_RS21105 at 5201.133 kb on - strand, within CA265_RS21105 at 5201.156 kb on + strand, within CA265_RS21105 at 5201.157 kb on - strand, within CA265_RS21105 at 5201.157 kb on - strand, within CA265_RS21105 at 5201.245 kb on + strand, within CA265_RS21105 at 5201.292 kb on + strand, within CA265_RS21105 at 5201.312 kb on - strand, within CA265_RS21105 at 5201.312 kb on - strand, within CA265_RS21105 at 5201.354 kb on - strand at 5201.381 kb on + strand at 5201.384 kb on + strand at 5201.392 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Chloride 300 mM remove 5,199,334 + CA265_RS21095 0.64 -0.9 5,199,407 + CA265_RS21095 0.67 -0.6 5,199,407 + CA265_RS21095 0.67 -0.3 5,199,408 - CA265_RS21095 0.67 +1.0 5,199,474 + CA265_RS21095 0.70 -0.9 5,199,475 - CA265_RS21095 0.70 +1.3 5,199,512 + -0.2 5,199,529 - CA265_RS21095 0.73 -0.3 5,199,549 + CA265_RS21095 0.73 +0.9 5,199,550 - CA265_RS21095 0.73 -0.4 5,199,550 - CA265_RS21095 0.73 +1.4 5,199,651 + CA265_RS21095 0.78 -0.2 5,199,651 + CA265_RS21095 0.78 +0.1 5,199,722 + CA265_RS21095 0.81 -1.2 5,199,723 - CA265_RS21095 0.81 +0.5 5,199,739 + CA265_RS21095 0.82 +4.6 5,199,739 + CA265_RS21095 0.82 -1.7 5,199,803 + CA265_RS21095 0.84 -2.3 5,199,913 + CA265_RS21095 0.89 -1.0 5,199,945 - +0.8 5,200,093 - +0.1 5,200,127 - -0.2 5,200,167 - -0.3 5,200,210 + -0.7 5,200,284 + +0.5 5,200,285 - -0.2 5,200,357 - CA265_RS21100 0.16 -0.6 5,200,453 - CA265_RS21100 0.41 -0.6 5,200,464 - CA265_RS21100 0.44 -0.3 5,200,509 + CA265_RS21100 0.56 -1.3 5,200,509 + CA265_RS21100 0.56 -1.6 5,200,510 - CA265_RS21100 0.56 -0.5 5,200,510 - CA265_RS21100 0.56 -0.7 5,200,533 - CA265_RS21100 0.62 -1.1 5,200,548 + CA265_RS21100 0.66 +0.9 5,200,577 - CA265_RS21100 0.73 -0.6 5,200,591 + CA265_RS21100 0.77 -0.7 5,200,777 + -0.4 5,200,847 - CA265_RS21105 0.16 +0.2 5,200,948 - CA265_RS21105 0.31 +0.8 5,200,948 - CA265_RS21105 0.31 -0.8 5,201,077 + CA265_RS21105 0.50 -0.1 5,201,077 + CA265_RS21105 0.50 -0.0 5,201,078 - CA265_RS21105 0.50 +0.1 5,201,125 + CA265_RS21105 0.58 -0.7 5,201,126 - CA265_RS21105 0.58 -0.2 5,201,133 - CA265_RS21105 0.59 +0.4 5,201,133 - CA265_RS21105 0.59 +1.0 5,201,156 + CA265_RS21105 0.62 +0.1 5,201,157 - CA265_RS21105 0.62 +0.0 5,201,157 - CA265_RS21105 0.62 +0.0 5,201,245 + CA265_RS21105 0.76 -0.3 5,201,292 + CA265_RS21105 0.83 -0.3 5,201,312 - CA265_RS21105 0.86 -0.3 5,201,312 - CA265_RS21105 0.86 +0.0 5,201,354 - +0.1 5,201,381 + -0.3 5,201,384 + -0.4 5,201,392 - +0.4
Or see this region's nucleotide sequence