Experiment: reconstituted MRS with D-Galactose 56 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS18475 and BBR_RS18480 are separated by 199 nucleotides BBR_RS18480 and BBR_RS18485 are separated by 80 nucleotides BBR_RS18485 and BBR_RS18490 are separated by 226 nucleotides
BBR_RS18475: BBR_RS18475 - DUF4981 domain-containing protein, at 1,930,417 to 1,933,554
_RS18475
BBR_RS18480: BBR_RS18480 - LacI family DNA-binding transcriptional regulator, at 1,933,754 to 1,934,764
_RS18480
BBR_RS18485: BBR_RS18485 - IS30 family transposase, at 1,934,845 to 1,935,042
_RS18485
BBR_RS18490: BBR_RS18490 - sugar ABC transporter substrate-binding protein, at 1,935,269 to 1,936,594
_RS18490
Position (kb) 1933
1934
1935 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4
5
6
7
8
9 at 1932.773 kb on - strand, within BBR_RS18475 at 1932.874 kb on - strand, within BBR_RS18475 at 1932.879 kb on - strand, within BBR_RS18475 at 1932.879 kb on - strand, within BBR_RS18475 at 1932.879 kb on - strand, within BBR_RS18475 at 1932.884 kb on - strand, within BBR_RS18475 at 1932.921 kb on - strand, within BBR_RS18475 at 1932.921 kb on - strand, within BBR_RS18475 at 1932.929 kb on + strand, within BBR_RS18475 at 1932.963 kb on - strand, within BBR_RS18475 at 1933.005 kb on + strand, within BBR_RS18475 at 1933.005 kb on + strand, within BBR_RS18475 at 1933.005 kb on + strand, within BBR_RS18475 at 1933.013 kb on - strand, within BBR_RS18475 at 1933.045 kb on - strand, within BBR_RS18475 at 1933.068 kb on + strand, within BBR_RS18475 at 1933.076 kb on - strand, within BBR_RS18475 at 1933.140 kb on + strand, within BBR_RS18475 at 1933.154 kb on + strand, within BBR_RS18475 at 1933.154 kb on + strand, within BBR_RS18475 at 1933.154 kb on + strand, within BBR_RS18475 at 1933.154 kb on + strand, within BBR_RS18475 at 1933.154 kb on + strand, within BBR_RS18475 at 1933.154 kb on + strand, within BBR_RS18475 at 1933.156 kb on + strand, within BBR_RS18475 at 1933.159 kb on + strand, within BBR_RS18475 at 1933.162 kb on - strand, within BBR_RS18475 at 1933.209 kb on - strand, within BBR_RS18475 at 1933.214 kb on + strand, within BBR_RS18475 at 1933.214 kb on + strand, within BBR_RS18475 at 1933.214 kb on + strand, within BBR_RS18475 at 1933.214 kb on + strand, within BBR_RS18475 at 1933.214 kb on + strand, within BBR_RS18475 at 1933.214 kb on + strand, within BBR_RS18475 at 1933.222 kb on - strand, within BBR_RS18475 at 1933.222 kb on - strand, within BBR_RS18475 at 1933.222 kb on - strand, within BBR_RS18475 at 1933.222 kb on - strand, within BBR_RS18475 at 1933.226 kb on + strand, within BBR_RS18475 at 1933.237 kb on - strand, within BBR_RS18475 at 1933.242 kb on - strand at 1933.246 kb on - strand at 1933.296 kb on - strand at 1933.316 kb on + strand at 1933.364 kb on + strand at 1933.367 kb on - strand at 1933.376 kb on - strand at 1933.397 kb on + strand at 1933.397 kb on + strand at 1933.402 kb on + strand at 1933.452 kb on - strand at 1933.470 kb on + strand at 1933.470 kb on + strand at 1933.470 kb on + strand at 1933.480 kb on - strand at 1933.519 kb on + strand at 1933.551 kb on - strand at 1933.556 kb on - strand at 1933.556 kb on - strand at 1933.556 kb on - strand at 1933.556 kb on - strand at 1933.633 kb on + strand at 1933.677 kb on - strand at 1933.680 kb on + strand at 1933.682 kb on + strand at 1933.682 kb on + strand at 1933.683 kb on - strand at 1933.683 kb on - strand at 1933.728 kb on + strand at 1933.728 kb on + strand at 1933.728 kb on + strand at 1933.728 kb on + strand at 1933.733 kb on + strand at 1933.733 kb on + strand at 1933.736 kb on - strand at 1933.766 kb on + strand at 1933.774 kb on + strand at 1933.779 kb on + strand at 1933.798 kb on + strand at 1933.810 kb on + strand at 1933.818 kb on - strand at 1933.847 kb on - strand at 1933.847 kb on - strand at 1933.885 kb on + strand, within BBR_RS18480 at 1933.885 kb on + strand, within BBR_RS18480 at 1933.920 kb on + strand, within BBR_RS18480 at 1933.922 kb on + strand, within BBR_RS18480 at 1933.922 kb on + strand, within BBR_RS18480 at 1933.928 kb on - strand, within BBR_RS18480 at 1933.928 kb on - strand, within BBR_RS18480 at 1933.928 kb on - strand, within BBR_RS18480 at 1933.978 kb on - strand, within BBR_RS18480 at 1933.978 kb on - strand, within BBR_RS18480 at 1934.047 kb on - strand, within BBR_RS18480 at 1934.056 kb on - strand, within BBR_RS18480 at 1934.140 kb on - strand, within BBR_RS18480 at 1934.141 kb on - strand, within BBR_RS18480 at 1934.145 kb on - strand, within BBR_RS18480 at 1934.145 kb on - strand, within BBR_RS18480 at 1934.162 kb on + strand, within BBR_RS18480 at 1934.162 kb on + strand, within BBR_RS18480 at 1934.169 kb on + strand, within BBR_RS18480 at 1934.170 kb on - strand, within BBR_RS18480 at 1934.170 kb on - strand, within BBR_RS18480 at 1934.259 kb on + strand, within BBR_RS18480 at 1934.269 kb on - strand, within BBR_RS18480 at 1934.300 kb on - strand, within BBR_RS18480 at 1934.302 kb on - strand, within BBR_RS18480 at 1934.309 kb on + strand, within BBR_RS18480 at 1934.333 kb on + strand, within BBR_RS18480 at 1934.360 kb on + strand, within BBR_RS18480 at 1934.368 kb on + strand, within BBR_RS18480 at 1934.375 kb on + strand, within BBR_RS18480 at 1934.375 kb on + strand, within BBR_RS18480 at 1934.375 kb on + strand, within BBR_RS18480 at 1934.376 kb on - strand, within BBR_RS18480 at 1934.378 kb on - strand, within BBR_RS18480 at 1934.383 kb on - strand, within BBR_RS18480 at 1934.383 kb on - strand, within BBR_RS18480 at 1934.383 kb on - strand, within BBR_RS18480 at 1934.383 kb on - strand, within BBR_RS18480 at 1934.409 kb on + strand, within BBR_RS18480 at 1934.411 kb on + strand, within BBR_RS18480 at 1934.419 kb on - strand, within BBR_RS18480 at 1934.419 kb on - strand, within BBR_RS18480 at 1934.419 kb on - strand, within BBR_RS18480 at 1934.427 kb on - strand, within BBR_RS18480 at 1934.427 kb on - strand, within BBR_RS18480 at 1934.441 kb on - strand, within BBR_RS18480 at 1934.499 kb on + strand, within BBR_RS18480 at 1934.501 kb on + strand, within BBR_RS18480 at 1934.509 kb on - strand, within BBR_RS18480 at 1934.509 kb on - strand, within BBR_RS18480 at 1934.509 kb on - strand, within BBR_RS18480 at 1934.534 kb on + strand, within BBR_RS18480 at 1934.553 kb on - strand, within BBR_RS18480 at 1934.562 kb on - strand, within BBR_RS18480 at 1934.563 kb on - strand, within BBR_RS18480 at 1934.563 kb on - strand, within BBR_RS18480 at 1934.563 kb on - strand, within BBR_RS18480 at 1934.630 kb on + strand, within BBR_RS18480 at 1934.651 kb on + strand, within BBR_RS18480 at 1934.682 kb on - strand at 1934.701 kb on + strand at 1934.720 kb on + strand at 1934.720 kb on + strand at 1934.727 kb on + strand at 1934.735 kb on - strand at 1934.855 kb on + strand at 1934.855 kb on - strand at 1934.863 kb on - strand at 1934.913 kb on + strand, within BBR_RS18485 at 1934.931 kb on - strand, within BBR_RS18485 at 1934.933 kb on + strand, within BBR_RS18485 at 1934.933 kb on + strand, within BBR_RS18485 at 1934.960 kb on + strand, within BBR_RS18485 at 1934.960 kb on + strand, within BBR_RS18485 at 1934.965 kb on + strand, within BBR_RS18485 at 1934.999 kb on - strand, within BBR_RS18485 at 1935.013 kb on - strand, within BBR_RS18485 at 1935.032 kb on + strand at 1935.033 kb on - strand at 1935.059 kb on + strand at 1935.085 kb on - strand at 1935.086 kb on - strand at 1935.086 kb on - strand at 1935.121 kb on - strand at 1935.141 kb on + strand at 1935.141 kb on + strand at 1935.149 kb on - strand at 1935.149 kb on - strand at 1935.149 kb on - strand at 1935.150 kb on - strand at 1935.150 kb on - strand at 1935.200 kb on - strand at 1935.214 kb on + strand at 1935.222 kb on - strand at 1935.237 kb on + strand at 1935.287 kb on - strand at 1935.289 kb on + strand at 1935.292 kb on - strand at 1935.322 kb on - strand at 1935.344 kb on - strand at 1935.347 kb on + strand at 1935.377 kb on - strand at 1935.388 kb on - strand at 1935.403 kb on + strand, within BBR_RS18490 at 1935.403 kb on + strand, within BBR_RS18490 at 1935.403 kb on + strand, within BBR_RS18490 at 1935.403 kb on + strand, within BBR_RS18490 at 1935.403 kb on + strand, within BBR_RS18490 at 1935.403 kb on + strand, within BBR_RS18490 at 1935.411 kb on - strand, within BBR_RS18490 at 1935.411 kb on - strand, within BBR_RS18490 at 1935.460 kb on - strand, within BBR_RS18490 at 1935.515 kb on - strand, within BBR_RS18490 at 1935.552 kb on - strand, within BBR_RS18490 at 1935.559 kb on - strand, within BBR_RS18490 at 1935.612 kb on + strand, within BBR_RS18490 at 1935.640 kb on - strand, within BBR_RS18490 at 1935.736 kb on - strand, within BBR_RS18490 at 1935.758 kb on + strand, within BBR_RS18490
Per-strain Table
Position Strand Gene LocusTag Fraction reconstituted MRS with D-Galactose 56 mM remove 1,932,773 - BBR_RS18475 0.75 -0.1 1,932,874 - BBR_RS18475 0.78 +0.3 1,932,879 - BBR_RS18475 0.78 +0.2 1,932,879 - BBR_RS18475 0.78 +0.5 1,932,879 - BBR_RS18475 0.78 -0.1 1,932,884 - BBR_RS18475 0.79 +0.9 1,932,921 - BBR_RS18475 0.80 +0.1 1,932,921 - BBR_RS18475 0.80 -0.6 1,932,929 + BBR_RS18475 0.80 +0.1 1,932,963 - BBR_RS18475 0.81 +0.1 1,933,005 + BBR_RS18475 0.82 +0.5 1,933,005 + BBR_RS18475 0.82 -0.2 1,933,005 + BBR_RS18475 0.82 -0.4 1,933,013 - BBR_RS18475 0.83 -0.9 1,933,045 - BBR_RS18475 0.84 +0.2 1,933,068 + BBR_RS18475 0.84 +0.7 1,933,076 - BBR_RS18475 0.85 +0.7 1,933,140 + BBR_RS18475 0.87 -0.5 1,933,154 + BBR_RS18475 0.87 +0.0 1,933,154 + BBR_RS18475 0.87 -0.2 1,933,154 + BBR_RS18475 0.87 -0.3 1,933,154 + BBR_RS18475 0.87 +0.2 1,933,154 + BBR_RS18475 0.87 -0.0 1,933,154 + BBR_RS18475 0.87 +0.2 1,933,156 + BBR_RS18475 0.87 +0.3 1,933,159 + BBR_RS18475 0.87 +0.4 1,933,162 - BBR_RS18475 0.87 -0.5 1,933,209 - BBR_RS18475 0.89 +0.5 1,933,214 + BBR_RS18475 0.89 +0.6 1,933,214 + BBR_RS18475 0.89 -0.0 1,933,214 + BBR_RS18475 0.89 +0.4 1,933,214 + BBR_RS18475 0.89 -0.1 1,933,214 + BBR_RS18475 0.89 +0.6 1,933,214 + BBR_RS18475 0.89 +0.0 1,933,222 - BBR_RS18475 0.89 +0.3 1,933,222 - BBR_RS18475 0.89 -0.3 1,933,222 - BBR_RS18475 0.89 +0.9 1,933,222 - BBR_RS18475 0.89 -0.2 1,933,226 + BBR_RS18475 0.90 -0.3 1,933,237 - BBR_RS18475 0.90 +0.1 1,933,242 - -0.5 1,933,246 - +0.0 1,933,296 - -0.0 1,933,316 + -0.2 1,933,364 + -0.3 1,933,367 - -0.2 1,933,376 - +9.1 1,933,397 + -1.3 1,933,397 + +0.5 1,933,402 + -0.0 1,933,452 - +0.1 1,933,470 + -0.3 1,933,470 + +0.3 1,933,470 + +0.1 1,933,480 - +0.8 1,933,519 + -0.1 1,933,551 - -1.9 1,933,556 - +0.5 1,933,556 - -0.1 1,933,556 - +0.2 1,933,556 - +0.2 1,933,633 + +0.4 1,933,677 - +0.2 1,933,680 + -0.8 1,933,682 + -0.7 1,933,682 + +0.4 1,933,683 - -0.6 1,933,683 - +0.2 1,933,728 + -1.2 1,933,728 + +0.6 1,933,728 + -1.1 1,933,728 + +0.6 1,933,733 + -0.1 1,933,733 + +0.3 1,933,736 - +1.1 1,933,766 + +9.5 1,933,774 + +0.6 1,933,779 + +1.8 1,933,798 + +9.2 1,933,810 + +9.6 1,933,818 - +1.6 1,933,847 - +8.8 1,933,847 - +9.1 1,933,885 + BBR_RS18480 0.13 +9.3 1,933,885 + BBR_RS18480 0.13 +9.4 1,933,920 + BBR_RS18480 0.16 +9.0 1,933,922 + BBR_RS18480 0.17 +9.4 1,933,922 + BBR_RS18480 0.17 +9.6 1,933,928 - BBR_RS18480 0.17 +9.3 1,933,928 - BBR_RS18480 0.17 +9.0 1,933,928 - BBR_RS18480 0.17 +8.6 1,933,978 - BBR_RS18480 0.22 +9.1 1,933,978 - BBR_RS18480 0.22 +9.1 1,934,047 - BBR_RS18480 0.29 +8.9 1,934,056 - BBR_RS18480 0.30 +9.4 1,934,140 - BBR_RS18480 0.38 +9.2 1,934,141 - BBR_RS18480 0.38 +8.9 1,934,145 - BBR_RS18480 0.39 +9.3 1,934,145 - BBR_RS18480 0.39 +9.4 1,934,162 + BBR_RS18480 0.40 +9.0 1,934,162 + BBR_RS18480 0.40 +8.8 1,934,169 + BBR_RS18480 0.41 +8.6 1,934,170 - BBR_RS18480 0.41 +8.9 1,934,170 - BBR_RS18480 0.41 +9.1 1,934,259 + BBR_RS18480 0.50 +9.3 1,934,269 - BBR_RS18480 0.51 +9.3 1,934,300 - BBR_RS18480 0.54 +9.4 1,934,302 - BBR_RS18480 0.54 +9.2 1,934,309 + BBR_RS18480 0.55 +9.3 1,934,333 + BBR_RS18480 0.57 +9.1 1,934,360 + BBR_RS18480 0.60 +9.5 1,934,368 + BBR_RS18480 0.61 +9.5 1,934,375 + BBR_RS18480 0.61 +8.6 1,934,375 + BBR_RS18480 0.61 +8.8 1,934,375 + BBR_RS18480 0.61 +9.0 1,934,376 - BBR_RS18480 0.62 +9.2 1,934,378 - BBR_RS18480 0.62 +9.5 1,934,383 - BBR_RS18480 0.62 +9.2 1,934,383 - BBR_RS18480 0.62 +9.7 1,934,383 - BBR_RS18480 0.62 +9.1 1,934,383 - BBR_RS18480 0.62 +9.3 1,934,409 + BBR_RS18480 0.65 +9.1 1,934,411 + BBR_RS18480 0.65 +9.1 1,934,419 - BBR_RS18480 0.66 +9.4 1,934,419 - BBR_RS18480 0.66 +9.6 1,934,419 - BBR_RS18480 0.66 +9.4 1,934,427 - BBR_RS18480 0.67 +9.6 1,934,427 - BBR_RS18480 0.67 +9.3 1,934,441 - BBR_RS18480 0.68 +9.3 1,934,499 + BBR_RS18480 0.74 +8.9 1,934,501 + BBR_RS18480 0.74 +9.2 1,934,509 - BBR_RS18480 0.75 +9.1 1,934,509 - BBR_RS18480 0.75 +9.3 1,934,509 - BBR_RS18480 0.75 +8.8 1,934,534 + BBR_RS18480 0.77 +9.4 1,934,553 - BBR_RS18480 0.79 +9.3 1,934,562 - BBR_RS18480 0.80 +9.1 1,934,563 - BBR_RS18480 0.80 +9.0 1,934,563 - BBR_RS18480 0.80 +9.4 1,934,563 - BBR_RS18480 0.80 +9.3 1,934,630 + BBR_RS18480 0.87 +9.1 1,934,651 + BBR_RS18480 0.89 +9.2 1,934,682 - +9.3 1,934,701 + +8.9 1,934,720 + +9.2 1,934,720 + +9.2 1,934,727 + +9.6 1,934,735 - +8.5 1,934,855 + -0.1 1,934,855 - +0.9 1,934,863 - +0.1 1,934,913 + BBR_RS18485 0.34 -0.1 1,934,931 - BBR_RS18485 0.43 +0.6 1,934,933 + BBR_RS18485 0.44 -0.3 1,934,933 + BBR_RS18485 0.44 +0.3 1,934,960 + BBR_RS18485 0.58 -0.9 1,934,960 + BBR_RS18485 0.58 +0.7 1,934,965 + BBR_RS18485 0.61 -0.3 1,934,999 - BBR_RS18485 0.78 -0.4 1,935,013 - BBR_RS18485 0.85 -0.1 1,935,032 + +0.5 1,935,033 - +0.3 1,935,059 + -0.3 1,935,085 - -0.2 1,935,086 - -0.9 1,935,086 - +0.2 1,935,121 - +0.4 1,935,141 + +0.2 1,935,141 + +0.7 1,935,149 - +0.8 1,935,149 - -0.1 1,935,149 - +0.3 1,935,150 - -0.4 1,935,150 - +0.3 1,935,200 - -0.5 1,935,214 + +0.8 1,935,222 - -0.1 1,935,237 + -0.1 1,935,287 - +0.0 1,935,289 + +0.2 1,935,292 - -1.0 1,935,322 - -0.1 1,935,344 - -0.0 1,935,347 + -0.3 1,935,377 - -0.4 1,935,388 - +0.1 1,935,403 + BBR_RS18490 0.10 -0.7 1,935,403 + BBR_RS18490 0.10 +0.6 1,935,403 + BBR_RS18490 0.10 -0.1 1,935,403 + BBR_RS18490 0.10 -0.6 1,935,403 + BBR_RS18490 0.10 +0.1 1,935,403 + BBR_RS18490 0.10 -0.9 1,935,411 - BBR_RS18490 0.11 +0.1 1,935,411 - BBR_RS18490 0.11 +0.6 1,935,460 - BBR_RS18490 0.14 -0.4 1,935,515 - BBR_RS18490 0.19 +0.3 1,935,552 - BBR_RS18490 0.21 +0.3 1,935,559 - BBR_RS18490 0.22 +0.0 1,935,612 + BBR_RS18490 0.26 -0.2 1,935,640 - BBR_RS18490 0.28 +1.5 1,935,736 - BBR_RS18490 0.35 +0.2 1,935,758 + BBR_RS18490 0.37 +0.8
Or see this region's nucleotide sequence