Experiment: Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; 1,3-Propanediol_timecourse3; 10 generations
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS06210 and MMP_RS06215 are separated by 209 nucleotides MMP_RS06215 and MMP_RS06220 are separated by 161 nucleotides
MMP_RS06210: MMP_RS06210 - type I glutamate--ammonia ligase, at 1,192,748 to 1,194,088
_RS06210
MMP_RS06215: MMP_RS06215 - 30S ribosomal protein S6e, at 1,194,298 to 1,194,672
_RS06215
MMP_RS06220: MMP_RS06220 - translation initiation factor IF-2 subunit gamma, at 1,194,834 to 1,196,066
_RS06220
Position (kb)
1194
1195 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1193.305 kb on + strand at 1193.557 kb on + strand at 1194.105 kb on + strand at 1194.116 kb on + strand at 1194.187 kb on + strand at 1194.192 kb on - strand at 1194.271 kb on - strand at 1194.271 kb on - strand at 1194.272 kb on + strand at 1194.510 kb on + strand, within MMP_RS06215 at 1194.510 kb on + strand, within MMP_RS06215 at 1194.597 kb on - strand, within MMP_RS06215 at 1194.719 kb on - strand at 1194.720 kb on + strand at 1194.721 kb on - strand at 1194.722 kb on + strand at 1194.724 kb on + strand at 1194.727 kb on - strand at 1194.727 kb on - strand at 1194.727 kb on - strand at 1194.728 kb on + strand at 1195.083 kb on + strand at 1195.513 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; 1,3-Propanediol_timecourse3; 10 generations remove 1,193,305 + -3.4 1,193,557 + -3.3 1,194,105 + -0.0 1,194,116 + -0.5 1,194,187 + -1.5 1,194,192 - -1.6 1,194,271 - -1.0 1,194,271 - -1.7 1,194,272 + -1.0 1,194,510 + MMP_RS06215 0.57 -2.5 1,194,510 + MMP_RS06215 0.57 -1.7 1,194,597 - MMP_RS06215 0.80 -2.7 1,194,719 - +0.1 1,194,720 + -0.7 1,194,721 - -0.2 1,194,722 + -0.1 1,194,724 + +0.0 1,194,727 - -1.7 1,194,727 - -1.9 1,194,727 - -1.9 1,194,728 + -1.6 1,195,083 + -3.3 1,195,513 - -3.3
Or see this region's nucleotide sequence