Experiment: Ammonium chloride (N); RCH2 media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS20205 and EX31_RS20210 are separated by 115 nucleotides EX31_RS20210 and EX31_RS20215 overlap by 11 nucleotides EX31_RS20215 and EX31_RS20220 overlap by 1 nucleotides
EX31_RS20205: EX31_RS20205 - cysteine synthase CysM, at 669,572 to 670,456
_RS20205
EX31_RS20210: EX31_RS20210 - sulfate/thiosulfate ABC transporter ATP-binding protein CysA, at 670,572 to 671,666
_RS20210
EX31_RS20215: EX31_RS20215 - sulfate/thiosulfate ABC transporter permease CysW, at 671,656 to 672,531
_RS20215
EX31_RS20220: EX31_RS20220 - sulfate/thiosulfate ABC transporter permease CysT, at 672,531 to 673,361
_RS20220
Position (kb) 670
671
672 Strain fitness (log2 ratio) -2
-1
0
1
2 at 669.600 kb on + strand at 669.601 kb on - strand at 669.601 kb on - strand at 669.601 kb on - strand at 669.601 kb on - strand at 669.622 kb on + strand at 669.623 kb on - strand at 669.623 kb on - strand at 669.666 kb on - strand, within EX31_RS20205 at 669.710 kb on - strand, within EX31_RS20205 at 669.710 kb on - strand, within EX31_RS20205 at 669.710 kb on - strand, within EX31_RS20205 at 669.710 kb on - strand, within EX31_RS20205 at 669.710 kb on - strand, within EX31_RS20205 at 669.710 kb on - strand, within EX31_RS20205 at 669.710 kb on - strand, within EX31_RS20205 at 669.810 kb on + strand, within EX31_RS20205 at 669.811 kb on - strand, within EX31_RS20205 at 669.811 kb on - strand, within EX31_RS20205 at 669.811 kb on - strand, within EX31_RS20205 at 670.074 kb on + strand, within EX31_RS20205 at 670.141 kb on - strand, within EX31_RS20205 at 670.141 kb on - strand, within EX31_RS20205 at 670.174 kb on + strand, within EX31_RS20205 at 670.175 kb on - strand, within EX31_RS20205 at 670.196 kb on - strand, within EX31_RS20205 at 670.196 kb on - strand, within EX31_RS20205 at 670.316 kb on - strand, within EX31_RS20205 at 670.355 kb on - strand, within EX31_RS20205 at 670.355 kb on - strand, within EX31_RS20205 at 670.409 kb on + strand at 670.409 kb on + strand at 670.409 kb on + strand at 670.409 kb on + strand at 670.410 kb on - strand at 670.410 kb on - strand at 670.410 kb on - strand at 670.427 kb on - strand at 670.437 kb on + strand at 670.437 kb on + strand at 670.437 kb on + strand at 670.437 kb on + strand at 670.438 kb on - strand at 670.451 kb on - strand at 670.480 kb on + strand at 670.481 kb on - strand at 670.483 kb on - strand at 670.503 kb on + strand at 670.504 kb on - strand at 670.602 kb on + strand at 670.621 kb on + strand at 670.622 kb on - strand at 670.622 kb on - strand at 670.622 kb on - strand at 670.622 kb on - strand at 670.622 kb on - strand at 670.622 kb on - strand at 670.622 kb on - strand at 670.648 kb on + strand at 670.649 kb on - strand at 670.649 kb on - strand at 670.677 kb on - strand at 670.743 kb on - strand, within EX31_RS20210 at 670.743 kb on - strand, within EX31_RS20210 at 670.897 kb on + strand, within EX31_RS20210 at 670.898 kb on - strand, within EX31_RS20210 at 670.898 kb on - strand, within EX31_RS20210 at 670.910 kb on - strand, within EX31_RS20210 at 670.944 kb on - strand, within EX31_RS20210 at 670.947 kb on - strand, within EX31_RS20210 at 671.028 kb on - strand, within EX31_RS20210 at 671.028 kb on - strand, within EX31_RS20210 at 671.028 kb on - strand, within EX31_RS20210 at 671.150 kb on + strand, within EX31_RS20210 at 671.403 kb on - strand, within EX31_RS20210 at 671.419 kb on + strand, within EX31_RS20210 at 671.420 kb on - strand, within EX31_RS20210 at 671.420 kb on - strand, within EX31_RS20210 at 671.526 kb on - strand, within EX31_RS20210 at 671.529 kb on - strand, within EX31_RS20210 at 671.606 kb on + strand at 671.607 kb on - strand at 671.607 kb on - strand at 671.607 kb on - strand at 671.621 kb on + strand at 671.622 kb on - strand at 671.622 kb on - strand at 671.636 kb on - strand at 671.645 kb on + strand at 671.795 kb on + strand, within EX31_RS20215 at 671.795 kb on + strand, within EX31_RS20215 at 671.795 kb on + strand, within EX31_RS20215 at 671.796 kb on - strand, within EX31_RS20215 at 671.796 kb on - strand, within EX31_RS20215 at 671.810 kb on - strand, within EX31_RS20215 at 671.810 kb on - strand, within EX31_RS20215 at 671.821 kb on + strand, within EX31_RS20215 at 671.821 kb on + strand, within EX31_RS20215 at 671.821 kb on + strand, within EX31_RS20215 at 671.822 kb on - strand, within EX31_RS20215 at 671.822 kb on - strand, within EX31_RS20215 at 671.822 kb on - strand, within EX31_RS20215 at 671.822 kb on - strand, within EX31_RS20215 at 671.822 kb on - strand, within EX31_RS20215 at 671.834 kb on + strand, within EX31_RS20215 at 671.834 kb on + strand, within EX31_RS20215 at 671.835 kb on - strand, within EX31_RS20215 at 671.835 kb on - strand, within EX31_RS20215 at 671.850 kb on + strand, within EX31_RS20215 at 671.851 kb on - strand, within EX31_RS20215 at 671.851 kb on - strand, within EX31_RS20215 at 671.918 kb on + strand, within EX31_RS20215 at 671.918 kb on + strand, within EX31_RS20215 at 671.919 kb on - strand, within EX31_RS20215 at 671.919 kb on - strand, within EX31_RS20215 at 671.923 kb on + strand, within EX31_RS20215 at 671.923 kb on + strand, within EX31_RS20215 at 671.923 kb on + strand, within EX31_RS20215 at 671.987 kb on - strand, within EX31_RS20215 at 672.070 kb on + strand, within EX31_RS20215 at 672.071 kb on - strand, within EX31_RS20215 at 672.071 kb on - strand, within EX31_RS20215 at 672.106 kb on - strand, within EX31_RS20215 at 672.106 kb on - strand, within EX31_RS20215 at 672.106 kb on - strand, within EX31_RS20215 at 672.106 kb on - strand, within EX31_RS20215 at 672.158 kb on + strand, within EX31_RS20215 at 672.159 kb on - strand, within EX31_RS20215 at 672.170 kb on + strand, within EX31_RS20215 at 672.170 kb on + strand, within EX31_RS20215 at 672.170 kb on + strand, within EX31_RS20215 at 672.170 kb on + strand, within EX31_RS20215 at 672.171 kb on - strand, within EX31_RS20215 at 672.171 kb on - strand, within EX31_RS20215 at 672.171 kb on - strand, within EX31_RS20215 at 672.171 kb on - strand, within EX31_RS20215 at 672.171 kb on - strand, within EX31_RS20215 at 672.171 kb on - strand, within EX31_RS20215 at 672.227 kb on - strand, within EX31_RS20215 at 672.230 kb on - strand, within EX31_RS20215 at 672.230 kb on - strand, within EX31_RS20215 at 672.230 kb on - strand, within EX31_RS20215 at 672.230 kb on - strand, within EX31_RS20215 at 672.336 kb on + strand, within EX31_RS20215 at 672.337 kb on - strand, within EX31_RS20215 at 672.337 kb on - strand, within EX31_RS20215 at 672.441 kb on + strand, within EX31_RS20215 at 672.441 kb on + strand, within EX31_RS20215 at 672.489 kb on + strand at 672.490 kb on - strand at 672.490 kb on - strand at 672.533 kb on - strand at 672.568 kb on - strand at 672.654 kb on + strand, within EX31_RS20220
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N); RCH2 media; shaking remove 669,600 + +0.3 669,601 - -0.7 669,601 - -0.6 669,601 - +0.3 669,601 - +0.1 669,622 + +0.0 669,623 - +0.8 669,623 - -0.2 669,666 - EX31_RS20205 0.11 -0.7 669,710 - EX31_RS20205 0.16 +1.6 669,710 - EX31_RS20205 0.16 -0.3 669,710 - EX31_RS20205 0.16 -0.8 669,710 - EX31_RS20205 0.16 -0.1 669,710 - EX31_RS20205 0.16 +0.5 669,710 - EX31_RS20205 0.16 -0.6 669,710 - EX31_RS20205 0.16 +0.0 669,810 + EX31_RS20205 0.27 -1.2 669,811 - EX31_RS20205 0.27 -0.5 669,811 - EX31_RS20205 0.27 -0.6 669,811 - EX31_RS20205 0.27 -0.6 670,074 + EX31_RS20205 0.57 +0.1 670,141 - EX31_RS20205 0.64 -0.4 670,141 - EX31_RS20205 0.64 -0.4 670,174 + EX31_RS20205 0.68 -0.7 670,175 - EX31_RS20205 0.68 -0.9 670,196 - EX31_RS20205 0.71 -0.0 670,196 - EX31_RS20205 0.71 -1.4 670,316 - EX31_RS20205 0.84 -0.1 670,355 - EX31_RS20205 0.88 +0.9 670,355 - EX31_RS20205 0.88 +0.1 670,409 + +1.4 670,409 + -0.3 670,409 + +0.0 670,409 + +0.0 670,410 - -1.0 670,410 - +0.4 670,410 - -1.3 670,427 - +0.1 670,437 + -1.1 670,437 + +2.0 670,437 + -1.1 670,437 + +1.0 670,438 - -0.3 670,451 - -0.5 670,480 + -2.2 670,481 - -1.6 670,483 - -0.8 670,503 + -0.4 670,504 - -1.3 670,602 + -0.8 670,621 + -1.3 670,622 - +0.5 670,622 - -1.0 670,622 - -0.9 670,622 - +0.4 670,622 - -0.7 670,622 - -0.8 670,622 - +1.0 670,648 + -0.2 670,649 - -0.9 670,649 - -1.9 670,677 - +0.1 670,743 - EX31_RS20210 0.16 +0.3 670,743 - EX31_RS20210 0.16 +1.0 670,897 + EX31_RS20210 0.30 +0.7 670,898 - EX31_RS20210 0.30 -0.4 670,898 - EX31_RS20210 0.30 -0.1 670,910 - EX31_RS20210 0.31 -2.0 670,944 - EX31_RS20210 0.34 +0.7 670,947 - EX31_RS20210 0.34 -0.9 671,028 - EX31_RS20210 0.42 -1.3 671,028 - EX31_RS20210 0.42 -0.4 671,028 - EX31_RS20210 0.42 -0.3 671,150 + EX31_RS20210 0.53 +0.8 671,403 - EX31_RS20210 0.76 -0.3 671,419 + EX31_RS20210 0.77 +0.0 671,420 - EX31_RS20210 0.77 -1.0 671,420 - EX31_RS20210 0.77 -1.6 671,526 - EX31_RS20210 0.87 -0.9 671,529 - EX31_RS20210 0.87 -1.8 671,606 + -1.6 671,607 - -2.1 671,607 - -1.4 671,607 - -0.7 671,621 + -0.8 671,622 - +0.2 671,622 - +0.1 671,636 - +0.9 671,645 + +0.7 671,795 + EX31_RS20215 0.16 -1.3 671,795 + EX31_RS20215 0.16 -0.4 671,795 + EX31_RS20215 0.16 -2.2 671,796 - EX31_RS20215 0.16 -2.4 671,796 - EX31_RS20215 0.16 -0.1 671,810 - EX31_RS20215 0.18 -0.6 671,810 - EX31_RS20215 0.18 +0.2 671,821 + EX31_RS20215 0.19 +0.2 671,821 + EX31_RS20215 0.19 -1.7 671,821 + EX31_RS20215 0.19 -1.7 671,822 - EX31_RS20215 0.19 -1.1 671,822 - EX31_RS20215 0.19 +2.0 671,822 - EX31_RS20215 0.19 -0.8 671,822 - EX31_RS20215 0.19 +0.3 671,822 - EX31_RS20215 0.19 -1.9 671,834 + EX31_RS20215 0.20 -1.4 671,834 + EX31_RS20215 0.20 -0.3 671,835 - EX31_RS20215 0.20 -1.7 671,835 - EX31_RS20215 0.20 -2.7 671,850 + EX31_RS20215 0.22 -2.1 671,851 - EX31_RS20215 0.22 -1.8 671,851 - EX31_RS20215 0.22 +0.9 671,918 + EX31_RS20215 0.30 -0.3 671,918 + EX31_RS20215 0.30 -1.0 671,919 - EX31_RS20215 0.30 +0.4 671,919 - EX31_RS20215 0.30 +0.9 671,923 + EX31_RS20215 0.30 +1.0 671,923 + EX31_RS20215 0.30 -0.1 671,923 + EX31_RS20215 0.30 +1.3 671,987 - EX31_RS20215 0.38 -0.8 672,070 + EX31_RS20215 0.47 -0.8 672,071 - EX31_RS20215 0.47 -1.1 672,071 - EX31_RS20215 0.47 +0.2 672,106 - EX31_RS20215 0.51 +0.4 672,106 - EX31_RS20215 0.51 -1.7 672,106 - EX31_RS20215 0.51 -0.1 672,106 - EX31_RS20215 0.51 +0.9 672,158 + EX31_RS20215 0.57 -1.3 672,159 - EX31_RS20215 0.57 -2.2 672,170 + EX31_RS20215 0.59 +0.3 672,170 + EX31_RS20215 0.59 -0.3 672,170 + EX31_RS20215 0.59 +1.6 672,170 + EX31_RS20215 0.59 -0.5 672,171 - EX31_RS20215 0.59 -0.6 672,171 - EX31_RS20215 0.59 -0.1 672,171 - EX31_RS20215 0.59 -0.6 672,171 - EX31_RS20215 0.59 +0.4 672,171 - EX31_RS20215 0.59 -0.6 672,171 - EX31_RS20215 0.59 -0.4 672,227 - EX31_RS20215 0.65 +0.2 672,230 - EX31_RS20215 0.66 -0.9 672,230 - EX31_RS20215 0.66 -0.5 672,230 - EX31_RS20215 0.66 -1.3 672,230 - EX31_RS20215 0.66 +1.1 672,336 + EX31_RS20215 0.78 +0.1 672,337 - EX31_RS20215 0.78 -0.3 672,337 - EX31_RS20215 0.78 -0.4 672,441 + EX31_RS20215 0.90 -0.1 672,441 + EX31_RS20215 0.90 +0.4 672,489 + -0.4 672,490 - +1.3 672,490 - -2.7 672,533 - -1.7 672,568 - -0.6 672,654 + EX31_RS20220 0.15 -0.4
Or see this region's nucleotide sequence