Experiment: Ammonium chloride (N); RCH2 media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS13770 and EX31_RS13775 are separated by 113 nucleotides EX31_RS13775 and EX31_RS13780 are separated by 194 nucleotides EX31_RS13780 and EX31_RS13785 are separated by 1 nucleotides EX31_RS13785 and EX31_RS13790 are separated by 9 nucleotides
EX31_RS13770: EX31_RS13770 - glycine zipper 2TM domain-containing protein, at 13,924 to 14,394
_RS13770
EX31_RS13775: EX31_RS13775 - transcriptional regulator SlyA, at 14,508 to 14,942
_RS13775
EX31_RS13780: EX31_RS13780 - DUF1656 domain-containing protein, at 15,137 to 15,361
_RS13780
EX31_RS13785: EX31_RS13785 - HlyD family secretion protein, at 15,363 to 16,220
_RS13785
EX31_RS13790: EX31_RS13790 - FUSC family protein, at 16,230 to 18,281
_RS13790
Position (kb) 15
16 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 14.175 kb on + strand, within EX31_RS13770 at 14.175 kb on + strand, within EX31_RS13770 at 14.258 kb on + strand, within EX31_RS13770 at 14.373 kb on + strand at 14.373 kb on + strand at 14.373 kb on + strand at 14.373 kb on + strand at 14.373 kb on + strand at 14.373 kb on + strand at 14.373 kb on + strand at 14.374 kb on - strand at 14.453 kb on - strand at 14.453 kb on - strand at 14.453 kb on - strand at 14.495 kb on + strand at 14.495 kb on + strand at 14.496 kb on - strand at 14.496 kb on - strand at 14.496 kb on - strand at 14.625 kb on - strand, within EX31_RS13775 at 14.625 kb on - strand, within EX31_RS13775 at 14.748 kb on - strand, within EX31_RS13775 at 14.820 kb on - strand, within EX31_RS13775 at 14.820 kb on - strand, within EX31_RS13775 at 14.908 kb on - strand at 14.962 kb on - strand at 14.970 kb on - strand at 14.995 kb on - strand at 15.010 kb on - strand at 15.022 kb on - strand at 15.029 kb on + strand at 15.030 kb on - strand at 15.030 kb on - strand at 15.030 kb on - strand at 15.030 kb on - strand at 15.030 kb on - strand at 15.030 kb on - strand at 15.031 kb on + strand at 15.032 kb on - strand at 15.032 kb on - strand at 15.033 kb on + strand at 15.033 kb on + strand at 15.033 kb on + strand at 15.033 kb on + strand at 15.033 kb on + strand at 15.033 kb on + strand at 15.034 kb on - strand at 15.034 kb on - strand at 15.034 kb on - strand at 15.034 kb on - strand at 15.034 kb on - strand at 15.034 kb on - strand at 15.034 kb on - strand at 15.034 kb on - strand at 15.034 kb on - strand at 15.034 kb on - strand at 15.034 kb on - strand at 15.035 kb on + strand at 15.035 kb on + strand at 15.035 kb on + strand at 15.035 kb on + strand at 15.035 kb on + strand at 15.035 kb on + strand at 15.035 kb on + strand at 15.035 kb on + strand at 15.035 kb on - strand at 15.036 kb on - strand at 15.036 kb on - strand at 15.036 kb on - strand at 15.036 kb on - strand at 15.036 kb on - strand at 15.036 kb on - strand at 15.036 kb on - strand at 15.036 kb on - strand at 15.036 kb on - strand at 15.036 kb on - strand at 15.036 kb on - strand at 15.038 kb on + strand at 15.038 kb on + strand at 15.038 kb on + strand at 15.038 kb on + strand at 15.038 kb on + strand at 15.039 kb on - strand at 15.039 kb on - strand at 15.039 kb on - strand at 15.039 kb on - strand at 15.039 kb on - strand at 15.039 kb on - strand at 15.039 kb on - strand at 15.039 kb on - strand at 15.039 kb on - strand at 15.039 kb on - strand at 15.039 kb on - strand at 15.041 kb on + strand at 15.041 kb on + strand at 15.041 kb on + strand at 15.042 kb on - strand at 15.042 kb on - strand at 15.042 kb on - strand at 15.042 kb on - strand at 15.042 kb on - strand at 15.042 kb on - strand at 15.084 kb on + strand at 15.085 kb on - strand at 15.094 kb on + strand at 15.094 kb on + strand at 15.095 kb on - strand at 15.095 kb on - strand at 15.095 kb on - strand at 15.095 kb on - strand at 15.095 kb on - strand at 15.096 kb on + strand at 15.122 kb on - strand at 15.216 kb on + strand, within EX31_RS13780 at 15.270 kb on - strand, within EX31_RS13780 at 15.307 kb on - strand, within EX31_RS13780 at 15.307 kb on - strand, within EX31_RS13780 at 15.325 kb on + strand, within EX31_RS13780 at 15.326 kb on - strand, within EX31_RS13780 at 15.346 kb on + strand at 15.393 kb on - strand at 15.444 kb on + strand at 15.447 kb on + strand at 15.449 kb on + strand, within EX31_RS13785 at 15.449 kb on + strand, within EX31_RS13785 at 15.450 kb on - strand, within EX31_RS13785 at 15.535 kb on + strand, within EX31_RS13785 at 15.540 kb on - strand, within EX31_RS13785 at 15.560 kb on + strand, within EX31_RS13785 at 15.560 kb on + strand, within EX31_RS13785 at 15.603 kb on - strand, within EX31_RS13785 at 15.603 kb on - strand, within EX31_RS13785 at 15.655 kb on + strand, within EX31_RS13785 at 15.708 kb on - strand, within EX31_RS13785 at 15.765 kb on + strand, within EX31_RS13785 at 15.787 kb on + strand, within EX31_RS13785 at 15.843 kb on + strand, within EX31_RS13785 at 15.843 kb on + strand, within EX31_RS13785 at 15.843 kb on + strand, within EX31_RS13785 at 15.844 kb on - strand, within EX31_RS13785 at 15.845 kb on + strand, within EX31_RS13785 at 15.845 kb on + strand, within EX31_RS13785 at 15.846 kb on - strand, within EX31_RS13785 at 15.846 kb on - strand, within EX31_RS13785 at 15.876 kb on + strand, within EX31_RS13785 at 15.876 kb on + strand, within EX31_RS13785 at 15.877 kb on - strand, within EX31_RS13785 at 15.877 kb on - strand, within EX31_RS13785 at 15.914 kb on + strand, within EX31_RS13785 at 15.928 kb on - strand, within EX31_RS13785 at 15.965 kb on + strand, within EX31_RS13785 at 15.997 kb on + strand, within EX31_RS13785 at 15.997 kb on + strand, within EX31_RS13785 at 15.997 kb on + strand, within EX31_RS13785 at 16.038 kb on - strand, within EX31_RS13785 at 16.049 kb on + strand, within EX31_RS13785 at 16.049 kb on + strand, within EX31_RS13785 at 16.054 kb on - strand, within EX31_RS13785 at 16.145 kb on + strand at 16.145 kb on + strand at 16.215 kb on - strand at 16.218 kb on + strand at 16.335 kb on + strand at 16.336 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N); RCH2 media; shaking remove 14,175 + EX31_RS13770 0.53 +0.1 14,175 + EX31_RS13770 0.53 +0.6 14,258 + EX31_RS13770 0.71 -2.9 14,373 + +0.4 14,373 + +0.6 14,373 + -0.5 14,373 + +0.3 14,373 + +0.1 14,373 + -1.7 14,373 + -0.1 14,374 - +2.1 14,453 - +1.4 14,453 - -1.3 14,453 - -1.4 14,495 + +0.4 14,495 + -0.9 14,496 - +0.2 14,496 - +0.3 14,496 - -0.2 14,625 - EX31_RS13775 0.27 -0.9 14,625 - EX31_RS13775 0.27 +1.9 14,748 - EX31_RS13775 0.55 +1.7 14,820 - EX31_RS13775 0.72 -0.1 14,820 - EX31_RS13775 0.72 +0.3 14,908 - -0.7 14,962 - -0.6 14,970 - +1.2 14,995 - +0.1 15,010 - +0.1 15,022 - -0.2 15,029 + +0.1 15,030 - -0.8 15,030 - -0.8 15,030 - -1.4 15,030 - +0.0 15,030 - +1.2 15,030 - -1.0 15,031 + -1.1 15,032 - +0.5 15,032 - -1.0 15,033 + +1.5 15,033 + +0.1 15,033 + -0.4 15,033 + -0.1 15,033 + +0.5 15,033 + -0.1 15,034 - -0.3 15,034 - +1.3 15,034 - +0.6 15,034 - +0.4 15,034 - -0.9 15,034 - -1.9 15,034 - +0.1 15,034 - +0.9 15,034 - -2.7 15,034 - +0.3 15,034 - +0.6 15,035 + +1.1 15,035 + -0.1 15,035 + +0.2 15,035 + +0.8 15,035 + -0.5 15,035 + +0.3 15,035 + +0.2 15,035 + +0.9 15,035 - -0.3 15,036 - -0.8 15,036 - +0.3 15,036 - +0.7 15,036 - -1.2 15,036 - +0.9 15,036 - -1.8 15,036 - +0.0 15,036 - -0.1 15,036 - +0.4 15,036 - +0.7 15,036 - -1.3 15,038 + +1.7 15,038 + -0.8 15,038 + -0.3 15,038 + +0.5 15,038 + +0.6 15,039 - -0.6 15,039 - -0.0 15,039 - -0.2 15,039 - -0.3 15,039 - -1.3 15,039 - +1.3 15,039 - +0.1 15,039 - -1.5 15,039 - +0.1 15,039 - -0.1 15,039 - +0.8 15,041 + +0.3 15,041 + -0.6 15,041 + +1.3 15,042 - +0.1 15,042 - +1.5 15,042 - +0.7 15,042 - -0.7 15,042 - -0.7 15,042 - -1.3 15,084 + +0.1 15,085 - +1.6 15,094 + -0.3 15,094 + +1.5 15,095 - +1.3 15,095 - -2.0 15,095 - -0.1 15,095 - -0.1 15,095 - +0.7 15,096 + +0.6 15,122 - -0.1 15,216 + EX31_RS13780 0.35 -2.0 15,270 - EX31_RS13780 0.59 +0.4 15,307 - EX31_RS13780 0.76 +1.1 15,307 - EX31_RS13780 0.76 -0.7 15,325 + EX31_RS13780 0.84 -0.8 15,326 - EX31_RS13780 0.84 -0.6 15,346 + +2.1 15,393 - +0.3 15,444 + -1.1 15,447 + -1.0 15,449 + EX31_RS13785 0.10 +1.3 15,449 + EX31_RS13785 0.10 -1.1 15,450 - EX31_RS13785 0.10 +1.7 15,535 + EX31_RS13785 0.20 -0.3 15,540 - EX31_RS13785 0.21 -1.3 15,560 + EX31_RS13785 0.23 -1.9 15,560 + EX31_RS13785 0.23 -0.7 15,603 - EX31_RS13785 0.28 +0.9 15,603 - EX31_RS13785 0.28 +0.3 15,655 + EX31_RS13785 0.34 -0.2 15,708 - EX31_RS13785 0.40 +0.4 15,765 + EX31_RS13785 0.47 +0.4 15,787 + EX31_RS13785 0.49 +0.5 15,843 + EX31_RS13785 0.56 -0.3 15,843 + EX31_RS13785 0.56 -2.2 15,843 + EX31_RS13785 0.56 -0.5 15,844 - EX31_RS13785 0.56 -0.2 15,845 + EX31_RS13785 0.56 +0.7 15,845 + EX31_RS13785 0.56 +1.0 15,846 - EX31_RS13785 0.56 -0.1 15,846 - EX31_RS13785 0.56 +0.3 15,876 + EX31_RS13785 0.60 +1.5 15,876 + EX31_RS13785 0.60 +0.4 15,877 - EX31_RS13785 0.60 -1.0 15,877 - EX31_RS13785 0.60 +0.7 15,914 + EX31_RS13785 0.64 +0.7 15,928 - EX31_RS13785 0.66 -1.8 15,965 + EX31_RS13785 0.70 +1.1 15,997 + EX31_RS13785 0.74 +0.9 15,997 + EX31_RS13785 0.74 +0.0 15,997 + EX31_RS13785 0.74 +0.9 16,038 - EX31_RS13785 0.79 -0.9 16,049 + EX31_RS13785 0.80 -1.3 16,049 + EX31_RS13785 0.80 +1.2 16,054 - EX31_RS13785 0.81 +0.4 16,145 + -1.1 16,145 + +1.3 16,215 - -0.1 16,218 + -0.7 16,335 + +0.0 16,336 - +0.5
Or see this region's nucleotide sequence