Experiment: Ammonium chloride (N); RCH2 media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS10140 and EX31_RS10145 are separated by 27 nucleotides EX31_RS10145 and EX31_RS10150 overlap by 1 nucleotides EX31_RS10150 and EX31_RS10155 overlap by 4 nucleotides
EX31_RS10140: EX31_RS10140 - glycosyl transferase, at 239,093 to 240,637
_RS10140
EX31_RS10145: EX31_RS10145 - ABC transporter permease, at 240,665 to 241,585
_RS10145
EX31_RS10150: EX31_RS10150 - ABC transporter permease, at 241,585 to 242,451
_RS10150
EX31_RS10155: EX31_RS10155 - ROK family protein, at 242,448 to 243,377
_RS10155
Position (kb) 240
241
242 Strain fitness (log2 ratio) -2
-1
0
1
2 at 239.717 kb on + strand, within EX31_RS10140 at 239.729 kb on - strand, within EX31_RS10140 at 239.752 kb on + strand, within EX31_RS10140 at 239.752 kb on + strand, within EX31_RS10140 at 239.752 kb on + strand, within EX31_RS10140 at 239.753 kb on - strand, within EX31_RS10140 at 239.753 kb on - strand, within EX31_RS10140 at 239.753 kb on - strand, within EX31_RS10140 at 239.753 kb on - strand, within EX31_RS10140 at 239.753 kb on - strand, within EX31_RS10140 at 239.969 kb on + strand, within EX31_RS10140 at 239.969 kb on + strand, within EX31_RS10140 at 239.970 kb on - strand, within EX31_RS10140 at 239.970 kb on - strand, within EX31_RS10140 at 240.368 kb on + strand, within EX31_RS10140 at 240.371 kb on + strand, within EX31_RS10140 at 240.372 kb on - strand, within EX31_RS10140 at 240.442 kb on - strand, within EX31_RS10140 at 240.489 kb on - strand at 240.511 kb on + strand at 240.539 kb on + strand at 240.539 kb on + strand at 240.557 kb on + strand at 240.574 kb on + strand at 240.648 kb on + strand at 240.649 kb on - strand at 240.649 kb on - strand at 240.649 kb on - strand at 240.649 kb on - strand at 240.660 kb on + strand at 240.674 kb on + strand at 240.675 kb on - strand at 240.858 kb on - strand, within EX31_RS10145 at 240.955 kb on + strand, within EX31_RS10145 at 241.274 kb on + strand, within EX31_RS10145 at 241.274 kb on + strand, within EX31_RS10145 at 241.274 kb on + strand, within EX31_RS10145 at 241.320 kb on - strand, within EX31_RS10145 at 241.475 kb on + strand, within EX31_RS10145 at 241.525 kb on - strand at 241.545 kb on + strand at 241.649 kb on + strand at 241.683 kb on + strand, within EX31_RS10150 at 241.683 kb on + strand, within EX31_RS10150 at 241.683 kb on + strand, within EX31_RS10150 at 241.684 kb on - strand, within EX31_RS10150 at 241.810 kb on + strand, within EX31_RS10150 at 241.840 kb on + strand, within EX31_RS10150 at 241.840 kb on + strand, within EX31_RS10150 at 241.840 kb on + strand, within EX31_RS10150 at 241.928 kb on + strand, within EX31_RS10150 at 242.019 kb on + strand, within EX31_RS10150 at 242.079 kb on - strand, within EX31_RS10150 at 242.169 kb on + strand, within EX31_RS10150 at 242.349 kb on + strand, within EX31_RS10150 at 242.349 kb on + strand, within EX31_RS10150 at 242.349 kb on + strand, within EX31_RS10150 at 242.349 kb on + strand, within EX31_RS10150 at 242.350 kb on - strand, within EX31_RS10150 at 242.354 kb on - strand, within EX31_RS10150 at 242.382 kb on + strand at 242.382 kb on + strand at 242.382 kb on + strand at 242.403 kb on + strand at 242.408 kb on + strand at 242.409 kb on - strand at 242.409 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N); RCH2 media; shaking remove 239,717 + EX31_RS10140 0.40 -1.6 239,729 - EX31_RS10140 0.41 +0.7 239,752 + EX31_RS10140 0.43 +0.9 239,752 + EX31_RS10140 0.43 +0.4 239,752 + EX31_RS10140 0.43 +0.2 239,753 - EX31_RS10140 0.43 -0.0 239,753 - EX31_RS10140 0.43 +0.3 239,753 - EX31_RS10140 0.43 -0.3 239,753 - EX31_RS10140 0.43 -0.1 239,753 - EX31_RS10140 0.43 +1.1 239,969 + EX31_RS10140 0.57 +0.7 239,969 + EX31_RS10140 0.57 -0.6 239,970 - EX31_RS10140 0.57 +0.9 239,970 - EX31_RS10140 0.57 -0.1 240,368 + EX31_RS10140 0.83 -0.6 240,371 + EX31_RS10140 0.83 -0.1 240,372 - EX31_RS10140 0.83 +2.4 240,442 - EX31_RS10140 0.87 +1.7 240,489 - +0.3 240,511 + +0.2 240,539 + +1.3 240,539 + +0.4 240,557 + +2.7 240,574 + +0.5 240,648 + +0.9 240,649 - +0.4 240,649 - +1.7 240,649 - +0.9 240,649 - -2.2 240,660 + +0.9 240,674 + +1.7 240,675 - -0.6 240,858 - EX31_RS10145 0.21 +0.6 240,955 + EX31_RS10145 0.31 -2.6 241,274 + EX31_RS10145 0.66 -0.1 241,274 + EX31_RS10145 0.66 +1.9 241,274 + EX31_RS10145 0.66 +0.0 241,320 - EX31_RS10145 0.71 +1.5 241,475 + EX31_RS10145 0.88 +1.1 241,525 - -0.9 241,545 + +1.4 241,649 + +0.6 241,683 + EX31_RS10150 0.11 +0.0 241,683 + EX31_RS10150 0.11 -1.5 241,683 + EX31_RS10150 0.11 -2.6 241,684 - EX31_RS10150 0.11 -0.2 241,810 + EX31_RS10150 0.26 +1.7 241,840 + EX31_RS10150 0.29 -0.1 241,840 + EX31_RS10150 0.29 +0.3 241,840 + EX31_RS10150 0.29 +0.1 241,928 + EX31_RS10150 0.40 +0.7 242,019 + EX31_RS10150 0.50 +1.3 242,079 - EX31_RS10150 0.57 -0.2 242,169 + EX31_RS10150 0.67 -0.6 242,349 + EX31_RS10150 0.88 +0.2 242,349 + EX31_RS10150 0.88 +0.9 242,349 + EX31_RS10150 0.88 -0.3 242,349 + EX31_RS10150 0.88 -0.2 242,350 - EX31_RS10150 0.88 +0.1 242,354 - EX31_RS10150 0.89 +0.9 242,382 + -0.1 242,382 + -0.1 242,382 + +1.1 242,403 + +1.1 242,408 + -1.2 242,409 - -0.6 242,409 - -0.1
Or see this region's nucleotide sequence