Experiment: Ammonium chloride (N); RCH2 media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS06475 and EX31_RS06480 overlap by 4 nucleotides EX31_RS06480 and EX31_RS06485 are separated by 56 nucleotides EX31_RS06485 and EX31_RS06490 are separated by 121 nucleotides EX31_RS06490 and EX31_RS06495 are separated by 301 nucleotides
EX31_RS06475: EX31_RS06475 - YdbH family protein, at 64,912 to 67,638
_RS06475
EX31_RS06480: EX31_RS06480 - YnbE family lipoprotein, at 67,635 to 67,829
_RS06480
EX31_RS06485: EX31_RS06485 - YdbL family protein, at 67,886 to 68,191
_RS06485
EX31_RS06490: EX31_RS06490 - FMN-dependent NADH-azoreductase, at 68,313 to 68,930
_RS06490
EX31_RS06495: EX31_RS06495 - ATP-dependent RNA helicase HrpA, at 69,232 to 73,107
_RS06495
Position (kb)
68
69 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 67.374 kb on + strand at 67.421 kb on - strand at 67.474 kb on + strand at 67.491 kb on + strand at 67.491 kb on + strand at 67.492 kb on - strand at 67.496 kb on - strand at 67.496 kb on - strand at 68.153 kb on + strand, within EX31_RS06485 at 68.153 kb on + strand, within EX31_RS06485 at 68.153 kb on + strand, within EX31_RS06485 at 68.173 kb on + strand at 68.174 kb on - strand at 68.189 kb on + strand at 68.189 kb on + strand at 68.237 kb on - strand at 68.279 kb on - strand at 68.279 kb on - strand at 68.310 kb on + strand at 68.310 kb on + strand at 68.311 kb on - strand at 68.311 kb on - strand at 68.311 kb on - strand at 68.314 kb on + strand at 68.314 kb on + strand at 68.315 kb on - strand at 68.315 kb on - strand at 68.395 kb on + strand, within EX31_RS06490 at 68.403 kb on - strand, within EX31_RS06490 at 68.425 kb on - strand, within EX31_RS06490 at 68.464 kb on + strand, within EX31_RS06490 at 68.465 kb on - strand, within EX31_RS06490 at 68.465 kb on - strand, within EX31_RS06490 at 68.465 kb on - strand, within EX31_RS06490 at 68.465 kb on - strand, within EX31_RS06490 at 68.473 kb on - strand, within EX31_RS06490 at 68.608 kb on + strand, within EX31_RS06490 at 68.641 kb on + strand, within EX31_RS06490 at 68.641 kb on + strand, within EX31_RS06490 at 68.641 kb on + strand, within EX31_RS06490 at 68.642 kb on - strand, within EX31_RS06490 at 68.699 kb on + strand, within EX31_RS06490 at 68.778 kb on + strand, within EX31_RS06490 at 68.778 kb on + strand, within EX31_RS06490 at 68.778 kb on + strand, within EX31_RS06490 at 68.779 kb on - strand, within EX31_RS06490 at 68.779 kb on - strand, within EX31_RS06490 at 68.779 kb on - strand, within EX31_RS06490 at 68.779 kb on - strand, within EX31_RS06490 at 68.781 kb on + strand, within EX31_RS06490 at 68.782 kb on - strand, within EX31_RS06490 at 68.782 kb on - strand, within EX31_RS06490 at 68.845 kb on + strand, within EX31_RS06490 at 68.920 kb on - strand at 68.944 kb on - strand at 68.954 kb on + strand at 68.954 kb on + strand at 68.955 kb on - strand at 68.955 kb on - strand at 68.958 kb on + strand at 68.958 kb on + strand at 68.959 kb on - strand at 68.974 kb on - strand at 68.975 kb on + strand at 68.976 kb on - strand at 69.003 kb on - strand at 69.003 kb on - strand at 69.003 kb on - strand at 69.003 kb on - strand at 69.003 kb on - strand at 69.042 kb on + strand at 69.043 kb on - strand at 69.043 kb on - strand at 69.114 kb on + strand at 69.124 kb on + strand at 69.129 kb on + strand at 69.130 kb on - strand at 69.130 kb on - strand at 69.281 kb on + strand at 69.281 kb on + strand at 69.281 kb on + strand at 69.281 kb on + strand at 69.281 kb on + strand at 69.281 kb on + strand at 69.282 kb on - strand at 69.334 kb on - strand at 69.478 kb on + strand at 69.478 kb on + strand at 69.478 kb on + strand at 69.478 kb on + strand at 69.478 kb on + strand at 69.478 kb on + strand at 69.478 kb on + strand at 69.478 kb on + strand at 69.478 kb on + strand at 69.479 kb on - strand at 69.479 kb on - strand at 69.479 kb on - strand at 69.479 kb on - strand at 69.479 kb on - strand at 69.479 kb on - strand at 69.479 kb on - strand at 69.503 kb on + strand at 69.503 kb on + strand at 69.503 kb on + strand at 69.600 kb on + strand at 69.661 kb on - strand, within EX31_RS06495 at 69.673 kb on - strand, within EX31_RS06495 at 69.688 kb on - strand, within EX31_RS06495 at 69.730 kb on - strand, within EX31_RS06495 at 69.747 kb on + strand, within EX31_RS06495 at 69.844 kb on - strand, within EX31_RS06495 at 69.854 kb on - strand, within EX31_RS06495 at 69.897 kb on + strand, within EX31_RS06495 at 69.897 kb on + strand, within EX31_RS06495 at 69.898 kb on - strand, within EX31_RS06495 at 69.912 kb on + strand, within EX31_RS06495
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N); RCH2 media; shaking remove 67,374 + +0.3 67,421 - -0.2 67,474 + -0.7 67,491 + -1.9 67,491 + -0.3 67,492 - -0.5 67,496 - -0.4 67,496 - +1.4 68,153 + EX31_RS06485 0.87 +1.3 68,153 + EX31_RS06485 0.87 +0.7 68,153 + EX31_RS06485 0.87 +0.4 68,173 + +1.6 68,174 - +0.4 68,189 + +0.4 68,189 + +1.6 68,237 - +1.3 68,279 - +0.5 68,279 - +1.6 68,310 + -1.3 68,310 + +0.2 68,311 - -1.2 68,311 - +0.5 68,311 - +1.1 68,314 + +0.8 68,314 + +0.8 68,315 - +0.2 68,315 - -0.5 68,395 + EX31_RS06490 0.13 +0.5 68,403 - EX31_RS06490 0.15 +0.6 68,425 - EX31_RS06490 0.18 -0.4 68,464 + EX31_RS06490 0.24 +0.6 68,465 - EX31_RS06490 0.25 +0.4 68,465 - EX31_RS06490 0.25 +0.7 68,465 - EX31_RS06490 0.25 +0.3 68,465 - EX31_RS06490 0.25 +0.3 68,473 - EX31_RS06490 0.26 +0.7 68,608 + EX31_RS06490 0.48 +0.8 68,641 + EX31_RS06490 0.53 -0.2 68,641 + EX31_RS06490 0.53 +0.0 68,641 + EX31_RS06490 0.53 +0.8 68,642 - EX31_RS06490 0.53 +0.6 68,699 + EX31_RS06490 0.62 -0.7 68,778 + EX31_RS06490 0.75 +0.4 68,778 + EX31_RS06490 0.75 +1.9 68,778 + EX31_RS06490 0.75 +0.2 68,779 - EX31_RS06490 0.75 +0.5 68,779 - EX31_RS06490 0.75 +1.6 68,779 - EX31_RS06490 0.75 -1.4 68,779 - EX31_RS06490 0.75 -0.1 68,781 + EX31_RS06490 0.76 +1.3 68,782 - EX31_RS06490 0.76 -0.8 68,782 - EX31_RS06490 0.76 +0.0 68,845 + EX31_RS06490 0.86 +1.0 68,920 - +1.6 68,944 - -0.1 68,954 + -1.1 68,954 + -0.5 68,955 - +0.0 68,955 - -1.2 68,958 + +1.9 68,958 + +0.8 68,959 - +0.6 68,974 - +1.6 68,975 + +0.0 68,976 - +1.6 69,003 - +0.7 69,003 - -0.4 69,003 - +0.9 69,003 - +2.2 69,003 - +0.9 69,042 + -0.6 69,043 - -0.4 69,043 - +0.0 69,114 + +2.0 69,124 + +0.3 69,129 + +0.2 69,130 - +0.0 69,130 - -0.6 69,281 + +1.8 69,281 + +0.3 69,281 + +1.3 69,281 + +1.2 69,281 + +1.3 69,281 + -1.2 69,282 - +0.9 69,334 - -2.6 69,478 + -0.5 69,478 + -2.1 69,478 + +0.3 69,478 + +0.1 69,478 + +1.2 69,478 + -0.3 69,478 + +0.0 69,478 + -0.4 69,478 + -0.8 69,479 - +0.2 69,479 - +0.1 69,479 - -0.3 69,479 - -0.4 69,479 - -0.6 69,479 - +1.8 69,479 - +0.6 69,503 + +0.1 69,503 + +0.5 69,503 + +0.2 69,600 + -1.2 69,661 - EX31_RS06495 0.11 +0.6 69,673 - EX31_RS06495 0.11 +1.2 69,688 - EX31_RS06495 0.12 +1.6 69,730 - EX31_RS06495 0.13 +0.7 69,747 + EX31_RS06495 0.13 +0.2 69,844 - EX31_RS06495 0.16 +0.6 69,854 - EX31_RS06495 0.16 -1.2 69,897 + EX31_RS06495 0.17 +0.5 69,897 + EX31_RS06495 0.17 -0.5 69,898 - EX31_RS06495 0.17 -1.2 69,912 + EX31_RS06495 0.18 -0.7
Or see this region's nucleotide sequence