Experiment: Ammonium chloride (N); RCH2 media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS04890 and EX31_RS04895 overlap by 11 nucleotides EX31_RS04895 and EX31_RS04900 are separated by 40 nucleotides EX31_RS04900 and EX31_RS04905 are separated by 5 nucleotides
EX31_RS04890: EX31_RS04890 - biotin-independent malonate decarboxylase subunit beta, at 104,373 to 105,215
_RS04890
EX31_RS04895: EX31_RS04895 - malonate decarboxylase subunit delta, at 105,205 to 105,507
_RS04895
EX31_RS04900: EX31_RS04900 - triphosphoribosyl-dephospho-CoA synthase, at 105,548 to 106,405
_RS04900
EX31_RS04905: EX31_RS04905 - malonate decarboxylase subunit alpha, at 106,411 to 108,072
_RS04905
Position (kb) 105
106
107 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 104.635 kb on + strand, within EX31_RS04890 at 104.635 kb on + strand, within EX31_RS04890 at 104.636 kb on - strand, within EX31_RS04890 at 104.636 kb on - strand, within EX31_RS04890 at 104.636 kb on - strand, within EX31_RS04890 at 104.636 kb on - strand, within EX31_RS04890 at 104.636 kb on - strand, within EX31_RS04890 at 104.713 kb on + strand, within EX31_RS04890 at 104.713 kb on + strand, within EX31_RS04890 at 104.713 kb on + strand, within EX31_RS04890 at 104.713 kb on + strand, within EX31_RS04890 at 104.714 kb on - strand, within EX31_RS04890 at 104.714 kb on - strand, within EX31_RS04890 at 104.714 kb on - strand, within EX31_RS04890 at 104.714 kb on - strand, within EX31_RS04890 at 104.714 kb on - strand, within EX31_RS04890 at 104.789 kb on + strand, within EX31_RS04890 at 104.799 kb on + strand, within EX31_RS04890 at 105.029 kb on + strand, within EX31_RS04890 at 105.104 kb on + strand, within EX31_RS04890 at 105.104 kb on + strand, within EX31_RS04890 at 105.104 kb on + strand, within EX31_RS04890 at 105.104 kb on + strand, within EX31_RS04890 at 105.104 kb on + strand, within EX31_RS04890 at 105.104 kb on + strand, within EX31_RS04890 at 105.104 kb on + strand, within EX31_RS04890 at 105.104 kb on + strand, within EX31_RS04890 at 105.104 kb on + strand, within EX31_RS04890 at 105.104 kb on + strand, within EX31_RS04890 at 105.104 kb on + strand, within EX31_RS04890 at 105.104 kb on + strand, within EX31_RS04890 at 105.104 kb on + strand, within EX31_RS04890 at 105.104 kb on + strand, within EX31_RS04890 at 105.104 kb on + strand, within EX31_RS04890 at 105.105 kb on - strand, within EX31_RS04890 at 105.105 kb on - strand, within EX31_RS04890 at 105.105 kb on - strand, within EX31_RS04890 at 105.105 kb on - strand, within EX31_RS04890 at 105.105 kb on - strand, within EX31_RS04890 at 105.105 kb on - strand, within EX31_RS04890 at 105.105 kb on - strand, within EX31_RS04890 at 105.105 kb on - strand, within EX31_RS04890 at 105.105 kb on - strand, within EX31_RS04890 at 105.105 kb on - strand, within EX31_RS04890 at 105.105 kb on - strand, within EX31_RS04890 at 105.105 kb on - strand, within EX31_RS04890 at 105.105 kb on - strand, within EX31_RS04890 at 105.203 kb on + strand at 105.346 kb on + strand, within EX31_RS04895 at 105.346 kb on + strand, within EX31_RS04895 at 105.347 kb on - strand, within EX31_RS04895 at 105.347 kb on - strand, within EX31_RS04895 at 105.347 kb on - strand, within EX31_RS04895 at 105.351 kb on + strand, within EX31_RS04895 at 105.351 kb on + strand, within EX31_RS04895 at 105.351 kb on + strand, within EX31_RS04895 at 105.351 kb on + strand, within EX31_RS04895 at 105.351 kb on + strand, within EX31_RS04895 at 105.351 kb on + strand, within EX31_RS04895 at 105.351 kb on + strand, within EX31_RS04895 at 105.351 kb on + strand, within EX31_RS04895 at 105.352 kb on - strand, within EX31_RS04895 at 105.352 kb on - strand, within EX31_RS04895 at 105.381 kb on + strand, within EX31_RS04895 at 105.413 kb on + strand, within EX31_RS04895 at 105.414 kb on - strand, within EX31_RS04895 at 105.414 kb on - strand, within EX31_RS04895 at 105.414 kb on - strand, within EX31_RS04895 at 105.414 kb on - strand, within EX31_RS04895 at 105.414 kb on - strand, within EX31_RS04895 at 105.414 kb on - strand, within EX31_RS04895 at 105.424 kb on + strand, within EX31_RS04895 at 105.424 kb on + strand, within EX31_RS04895 at 105.425 kb on - strand, within EX31_RS04895 at 105.425 kb on - strand, within EX31_RS04895 at 105.425 kb on - strand, within EX31_RS04895 at 105.439 kb on + strand, within EX31_RS04895 at 105.439 kb on + strand, within EX31_RS04895 at 105.440 kb on - strand, within EX31_RS04895 at 105.486 kb on - strand at 105.489 kb on + strand at 105.489 kb on + strand at 105.489 kb on + strand at 105.489 kb on + strand at 105.508 kb on - strand at 105.508 kb on - strand at 105.520 kb on + strand at 105.520 kb on + strand at 105.521 kb on - strand at 105.529 kb on + strand at 105.529 kb on + strand at 105.529 kb on + strand at 105.529 kb on + strand at 105.529 kb on + strand at 105.529 kb on + strand at 105.529 kb on + strand at 105.529 kb on + strand at 105.529 kb on + strand at 105.530 kb on - strand at 105.530 kb on - strand at 105.530 kb on - strand at 105.530 kb on - strand at 105.530 kb on - strand at 105.530 kb on - strand at 105.530 kb on - strand at 105.533 kb on + strand at 105.533 kb on + strand at 105.533 kb on + strand at 105.534 kb on - strand at 105.534 kb on - strand at 105.534 kb on - strand at 105.590 kb on + strand at 105.591 kb on - strand at 105.591 kb on - strand at 105.788 kb on + strand, within EX31_RS04900 at 105.788 kb on + strand, within EX31_RS04900 at 105.788 kb on + strand, within EX31_RS04900 at 105.789 kb on - strand, within EX31_RS04900 at 105.915 kb on + strand, within EX31_RS04900 at 105.941 kb on - strand, within EX31_RS04900 at 106.003 kb on + strand, within EX31_RS04900 at 106.003 kb on + strand, within EX31_RS04900 at 106.003 kb on + strand, within EX31_RS04900 at 106.004 kb on - strand, within EX31_RS04900 at 106.004 kb on - strand, within EX31_RS04900 at 106.069 kb on + strand, within EX31_RS04900 at 106.069 kb on + strand, within EX31_RS04900 at 106.084 kb on + strand, within EX31_RS04900 at 106.085 kb on - strand, within EX31_RS04900 at 106.134 kb on - strand, within EX31_RS04900 at 106.134 kb on - strand, within EX31_RS04900 at 106.134 kb on - strand, within EX31_RS04900 at 106.188 kb on - strand, within EX31_RS04900 at 106.200 kb on + strand, within EX31_RS04900 at 106.200 kb on + strand, within EX31_RS04900 at 106.200 kb on + strand, within EX31_RS04900 at 106.200 kb on + strand, within EX31_RS04900 at 106.201 kb on - strand, within EX31_RS04900 at 106.201 kb on - strand, within EX31_RS04900 at 106.201 kb on - strand, within EX31_RS04900 at 106.201 kb on - strand, within EX31_RS04900 at 106.201 kb on - strand, within EX31_RS04900 at 106.201 kb on - strand, within EX31_RS04900 at 106.244 kb on + strand, within EX31_RS04900 at 106.244 kb on + strand, within EX31_RS04900 at 106.244 kb on + strand, within EX31_RS04900 at 106.245 kb on - strand, within EX31_RS04900 at 106.245 kb on - strand, within EX31_RS04900 at 106.250 kb on + strand, within EX31_RS04900 at 106.250 kb on + strand, within EX31_RS04900 at 106.250 kb on + strand, within EX31_RS04900 at 106.250 kb on + strand, within EX31_RS04900 at 106.250 kb on + strand, within EX31_RS04900 at 106.250 kb on + strand, within EX31_RS04900 at 106.250 kb on + strand, within EX31_RS04900 at 106.250 kb on + strand, within EX31_RS04900 at 106.250 kb on + strand, within EX31_RS04900 at 106.250 kb on + strand, within EX31_RS04900 at 106.251 kb on - strand, within EX31_RS04900 at 106.284 kb on - strand, within EX31_RS04900 at 106.405 kb on + strand at 106.405 kb on + strand at 106.406 kb on - strand at 106.406 kb on - strand at 106.406 kb on - strand at 106.406 kb on - strand at 106.441 kb on - strand at 106.442 kb on + strand at 106.442 kb on + strand at 106.442 kb on + strand at 106.442 kb on + strand at 106.442 kb on + strand at 106.442 kb on + strand at 106.442 kb on + strand at 106.442 kb on + strand at 106.442 kb on + strand at 106.443 kb on - strand at 106.443 kb on - strand at 106.443 kb on - strand at 106.443 kb on - strand at 106.443 kb on - strand at 106.443 kb on - strand at 106.443 kb on - strand at 106.443 kb on - strand at 106.443 kb on - strand at 106.443 kb on - strand at 106.527 kb on + strand at 106.528 kb on - strand at 106.667 kb on + strand, within EX31_RS04905 at 106.675 kb on - strand, within EX31_RS04905 at 106.675 kb on - strand, within EX31_RS04905 at 106.712 kb on + strand, within EX31_RS04905 at 106.713 kb on - strand, within EX31_RS04905 at 106.713 kb on - strand, within EX31_RS04905 at 106.822 kb on + strand, within EX31_RS04905 at 106.823 kb on - strand, within EX31_RS04905 at 106.823 kb on - strand, within EX31_RS04905 at 106.823 kb on - strand, within EX31_RS04905 at 106.823 kb on - strand, within EX31_RS04905 at 106.823 kb on - strand, within EX31_RS04905 at 106.869 kb on + strand, within EX31_RS04905 at 106.923 kb on + strand, within EX31_RS04905 at 107.064 kb on + strand, within EX31_RS04905 at 107.064 kb on + strand, within EX31_RS04905 at 107.064 kb on + strand, within EX31_RS04905 at 107.064 kb on + strand, within EX31_RS04905 at 107.065 kb on - strand, within EX31_RS04905 at 107.065 kb on - strand, within EX31_RS04905 at 107.065 kb on - strand, within EX31_RS04905 at 107.065 kb on - strand, within EX31_RS04905 at 107.065 kb on - strand, within EX31_RS04905 at 107.065 kb on - strand, within EX31_RS04905 at 107.065 kb on - strand, within EX31_RS04905 at 107.111 kb on + strand, within EX31_RS04905 at 107.111 kb on + strand, within EX31_RS04905 at 107.111 kb on + strand, within EX31_RS04905 at 107.111 kb on + strand, within EX31_RS04905 at 107.112 kb on - strand, within EX31_RS04905 at 107.112 kb on - strand, within EX31_RS04905 at 107.112 kb on - strand, within EX31_RS04905 at 107.112 kb on - strand, within EX31_RS04905 at 107.249 kb on + strand, within EX31_RS04905 at 107.284 kb on - strand, within EX31_RS04905 at 107.327 kb on + strand, within EX31_RS04905 at 107.327 kb on + strand, within EX31_RS04905 at 107.327 kb on + strand, within EX31_RS04905 at 107.327 kb on + strand, within EX31_RS04905 at 107.327 kb on + strand, within EX31_RS04905 at 107.328 kb on - strand, within EX31_RS04905 at 107.328 kb on - strand, within EX31_RS04905 at 107.328 kb on - strand, within EX31_RS04905 at 107.328 kb on - strand, within EX31_RS04905 at 107.328 kb on - strand, within EX31_RS04905 at 107.331 kb on + strand, within EX31_RS04905 at 107.331 kb on + strand, within EX31_RS04905 at 107.331 kb on + strand, within EX31_RS04905 at 107.332 kb on - strand, within EX31_RS04905 at 107.332 kb on - strand, within EX31_RS04905 at 107.332 kb on - strand, within EX31_RS04905 at 107.332 kb on - strand, within EX31_RS04905 at 107.337 kb on + strand, within EX31_RS04905
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N); RCH2 media; shaking remove 104,635 + EX31_RS04890 0.31 +0.6 104,635 + EX31_RS04890 0.31 +0.6 104,636 - EX31_RS04890 0.31 +0.1 104,636 - EX31_RS04890 0.31 +0.3 104,636 - EX31_RS04890 0.31 +0.2 104,636 - EX31_RS04890 0.31 -0.4 104,636 - EX31_RS04890 0.31 +1.0 104,713 + EX31_RS04890 0.40 +0.5 104,713 + EX31_RS04890 0.40 +0.1 104,713 + EX31_RS04890 0.40 -0.3 104,713 + EX31_RS04890 0.40 +0.0 104,714 - EX31_RS04890 0.40 -0.8 104,714 - EX31_RS04890 0.40 +0.4 104,714 - EX31_RS04890 0.40 +0.2 104,714 - EX31_RS04890 0.40 -1.4 104,714 - EX31_RS04890 0.40 +0.5 104,789 + EX31_RS04890 0.49 +0.5 104,799 + EX31_RS04890 0.51 +0.1 105,029 + EX31_RS04890 0.78 +1.5 105,104 + EX31_RS04890 0.87 -0.0 105,104 + EX31_RS04890 0.87 +1.1 105,104 + EX31_RS04890 0.87 +1.6 105,104 + EX31_RS04890 0.87 +0.8 105,104 + EX31_RS04890 0.87 -0.5 105,104 + EX31_RS04890 0.87 +0.7 105,104 + EX31_RS04890 0.87 +1.0 105,104 + EX31_RS04890 0.87 +0.5 105,104 + EX31_RS04890 0.87 -1.5 105,104 + EX31_RS04890 0.87 +0.8 105,104 + EX31_RS04890 0.87 +1.0 105,104 + EX31_RS04890 0.87 +0.4 105,104 + EX31_RS04890 0.87 +1.7 105,104 + EX31_RS04890 0.87 +0.6 105,104 + EX31_RS04890 0.87 +0.5 105,105 - EX31_RS04890 0.87 -0.1 105,105 - EX31_RS04890 0.87 +0.7 105,105 - EX31_RS04890 0.87 +0.2 105,105 - EX31_RS04890 0.87 -0.2 105,105 - EX31_RS04890 0.87 +1.5 105,105 - EX31_RS04890 0.87 +0.8 105,105 - EX31_RS04890 0.87 +0.8 105,105 - EX31_RS04890 0.87 -0.3 105,105 - EX31_RS04890 0.87 -2.0 105,105 - EX31_RS04890 0.87 -0.4 105,105 - EX31_RS04890 0.87 -0.3 105,105 - EX31_RS04890 0.87 -0.6 105,105 - EX31_RS04890 0.87 -2.2 105,203 + +0.1 105,346 + EX31_RS04895 0.47 +0.9 105,346 + EX31_RS04895 0.47 +0.4 105,347 - EX31_RS04895 0.47 -0.1 105,347 - EX31_RS04895 0.47 +0.5 105,347 - EX31_RS04895 0.47 -0.1 105,351 + EX31_RS04895 0.48 -0.2 105,351 + EX31_RS04895 0.48 +1.5 105,351 + EX31_RS04895 0.48 -0.4 105,351 + EX31_RS04895 0.48 +0.7 105,351 + EX31_RS04895 0.48 +0.3 105,351 + EX31_RS04895 0.48 +0.1 105,351 + EX31_RS04895 0.48 +0.1 105,351 + EX31_RS04895 0.48 -1.5 105,352 - EX31_RS04895 0.49 +2.5 105,352 - EX31_RS04895 0.49 -0.2 105,381 + EX31_RS04895 0.58 -0.3 105,413 + EX31_RS04895 0.69 +1.0 105,414 - EX31_RS04895 0.69 -0.3 105,414 - EX31_RS04895 0.69 -0.6 105,414 - EX31_RS04895 0.69 -0.5 105,414 - EX31_RS04895 0.69 +1.8 105,414 - EX31_RS04895 0.69 -0.2 105,414 - EX31_RS04895 0.69 +0.2 105,424 + EX31_RS04895 0.72 -0.5 105,424 + EX31_RS04895 0.72 -0.3 105,425 - EX31_RS04895 0.73 -1.0 105,425 - EX31_RS04895 0.73 +0.3 105,425 - EX31_RS04895 0.73 -0.3 105,439 + EX31_RS04895 0.77 -0.4 105,439 + EX31_RS04895 0.77 -0.3 105,440 - EX31_RS04895 0.78 +0.5 105,486 - +0.5 105,489 + +0.2 105,489 + -0.2 105,489 + +0.3 105,489 + -1.5 105,508 - +0.2 105,508 - +2.1 105,520 + +1.5 105,520 + +0.2 105,521 - +1.0 105,529 + +1.0 105,529 + +0.7 105,529 + +0.1 105,529 + -0.3 105,529 + +0.9 105,529 + +0.7 105,529 + +0.4 105,529 + +0.9 105,529 + +0.2 105,530 - -0.8 105,530 - -1.1 105,530 - -0.4 105,530 - -0.4 105,530 - +0.4 105,530 - +0.7 105,530 - +2.5 105,533 + +0.4 105,533 + -0.9 105,533 + +0.1 105,534 - +0.2 105,534 - -1.0 105,534 - -0.0 105,590 + -0.6 105,591 - -0.8 105,591 - +0.1 105,788 + EX31_RS04900 0.28 +0.4 105,788 + EX31_RS04900 0.28 +0.7 105,788 + EX31_RS04900 0.28 +0.1 105,789 - EX31_RS04900 0.28 +0.4 105,915 + EX31_RS04900 0.43 +0.3 105,941 - EX31_RS04900 0.46 +0.2 106,003 + EX31_RS04900 0.53 +0.8 106,003 + EX31_RS04900 0.53 -0.9 106,003 + EX31_RS04900 0.53 -0.2 106,004 - EX31_RS04900 0.53 -1.0 106,004 - EX31_RS04900 0.53 -0.2 106,069 + EX31_RS04900 0.61 +0.6 106,069 + EX31_RS04900 0.61 +1.2 106,084 + EX31_RS04900 0.62 +2.5 106,085 - EX31_RS04900 0.63 +1.2 106,134 - EX31_RS04900 0.68 +0.6 106,134 - EX31_RS04900 0.68 +1.0 106,134 - EX31_RS04900 0.68 +0.0 106,188 - EX31_RS04900 0.75 +0.2 106,200 + EX31_RS04900 0.76 +1.3 106,200 + EX31_RS04900 0.76 +0.0 106,200 + EX31_RS04900 0.76 -0.5 106,200 + EX31_RS04900 0.76 -1.3 106,201 - EX31_RS04900 0.76 +0.4 106,201 - EX31_RS04900 0.76 +0.4 106,201 - EX31_RS04900 0.76 -1.3 106,201 - EX31_RS04900 0.76 +0.7 106,201 - EX31_RS04900 0.76 -0.4 106,201 - EX31_RS04900 0.76 +0.1 106,244 + EX31_RS04900 0.81 +0.7 106,244 + EX31_RS04900 0.81 -0.1 106,244 + EX31_RS04900 0.81 -3.0 106,245 - EX31_RS04900 0.81 -0.0 106,245 - EX31_RS04900 0.81 +0.2 106,250 + EX31_RS04900 0.82 +1.5 106,250 + EX31_RS04900 0.82 -0.3 106,250 + EX31_RS04900 0.82 +0.5 106,250 + EX31_RS04900 0.82 -0.1 106,250 + EX31_RS04900 0.82 -1.2 106,250 + EX31_RS04900 0.82 -0.4 106,250 + EX31_RS04900 0.82 +0.7 106,250 + EX31_RS04900 0.82 -0.5 106,250 + EX31_RS04900 0.82 +0.9 106,250 + EX31_RS04900 0.82 +0.3 106,251 - EX31_RS04900 0.82 +0.2 106,284 - EX31_RS04900 0.86 +0.3 106,405 + -0.6 106,405 + -0.4 106,406 - +0.2 106,406 - -0.4 106,406 - +0.9 106,406 - -0.2 106,441 - +0.1 106,442 + -0.3 106,442 + -0.1 106,442 + +0.5 106,442 + +0.1 106,442 + +0.7 106,442 + +0.4 106,442 + +0.2 106,442 + -0.3 106,442 + +1.0 106,443 - +1.0 106,443 - +0.1 106,443 - +0.5 106,443 - +0.5 106,443 - -0.1 106,443 - +0.4 106,443 - -0.1 106,443 - -1.4 106,443 - +0.2 106,443 - -1.2 106,527 + -0.3 106,528 - -0.4 106,667 + EX31_RS04905 0.15 -1.5 106,675 - EX31_RS04905 0.16 -0.7 106,675 - EX31_RS04905 0.16 +1.1 106,712 + EX31_RS04905 0.18 -0.6 106,713 - EX31_RS04905 0.18 +0.1 106,713 - EX31_RS04905 0.18 -0.7 106,822 + EX31_RS04905 0.25 -1.3 106,823 - EX31_RS04905 0.25 -2.5 106,823 - EX31_RS04905 0.25 +1.7 106,823 - EX31_RS04905 0.25 +0.6 106,823 - EX31_RS04905 0.25 +0.3 106,823 - EX31_RS04905 0.25 -1.1 106,869 + EX31_RS04905 0.28 +0.3 106,923 + EX31_RS04905 0.31 +1.0 107,064 + EX31_RS04905 0.39 -0.6 107,064 + EX31_RS04905 0.39 -0.0 107,064 + EX31_RS04905 0.39 +0.4 107,064 + EX31_RS04905 0.39 -0.4 107,065 - EX31_RS04905 0.39 +0.8 107,065 - EX31_RS04905 0.39 +0.2 107,065 - EX31_RS04905 0.39 +0.8 107,065 - EX31_RS04905 0.39 -0.2 107,065 - EX31_RS04905 0.39 -0.2 107,065 - EX31_RS04905 0.39 -0.7 107,065 - EX31_RS04905 0.39 -0.6 107,111 + EX31_RS04905 0.42 +0.3 107,111 + EX31_RS04905 0.42 +1.0 107,111 + EX31_RS04905 0.42 +0.2 107,111 + EX31_RS04905 0.42 +0.1 107,112 - EX31_RS04905 0.42 -0.3 107,112 - EX31_RS04905 0.42 +1.0 107,112 - EX31_RS04905 0.42 +0.0 107,112 - EX31_RS04905 0.42 -0.1 107,249 + EX31_RS04905 0.50 +1.0 107,284 - EX31_RS04905 0.53 +0.4 107,327 + EX31_RS04905 0.55 -0.1 107,327 + EX31_RS04905 0.55 +0.1 107,327 + EX31_RS04905 0.55 -0.7 107,327 + EX31_RS04905 0.55 +0.3 107,327 + EX31_RS04905 0.55 -0.2 107,328 - EX31_RS04905 0.55 -0.0 107,328 - EX31_RS04905 0.55 +0.7 107,328 - EX31_RS04905 0.55 -0.1 107,328 - EX31_RS04905 0.55 +1.0 107,328 - EX31_RS04905 0.55 -1.3 107,331 + EX31_RS04905 0.55 +0.5 107,331 + EX31_RS04905 0.55 +0.5 107,331 + EX31_RS04905 0.55 -0.9 107,332 - EX31_RS04905 0.55 +0.2 107,332 - EX31_RS04905 0.55 -1.0 107,332 - EX31_RS04905 0.55 -0.3 107,332 - EX31_RS04905 0.55 +0.3 107,337 + EX31_RS04905 0.56 +0.5
Or see this region's nucleotide sequence