Experiment: Ammonium chloride (N); RCH2 media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS03260 and EX31_RS03265 overlap by 4 nucleotides EX31_RS03265 and EX31_RS03270 are separated by 5 nucleotides
EX31_RS03260: EX31_RS03260 - polynucleotide adenylyltransferase PcnB, at 142,836 to 144,542
_RS03260
EX31_RS03265: EX31_RS03265 - 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine diphosphokinase, at 144,539 to 145,018
_RS03265
EX31_RS03270: EX31_RS03270 - two-component system sensor histidine kinase QseC, at 145,024 to 146,409
_RS03270
Position (kb)
144
145
146 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 143.545 kb on + strand, within EX31_RS03260 at 143.643 kb on + strand, within EX31_RS03260 at 143.643 kb on + strand, within EX31_RS03260 at 143.643 kb on + strand, within EX31_RS03260 at 143.646 kb on + strand, within EX31_RS03260 at 143.668 kb on + strand, within EX31_RS03260 at 143.668 kb on + strand, within EX31_RS03260 at 143.668 kb on + strand, within EX31_RS03260 at 143.668 kb on + strand, within EX31_RS03260 at 143.669 kb on - strand, within EX31_RS03260 at 143.676 kb on + strand, within EX31_RS03260 at 143.676 kb on + strand, within EX31_RS03260 at 143.762 kb on + strand, within EX31_RS03260 at 143.762 kb on + strand, within EX31_RS03260 at 143.762 kb on + strand, within EX31_RS03260 at 143.777 kb on - strand, within EX31_RS03260 at 143.925 kb on + strand, within EX31_RS03260 at 143.927 kb on + strand, within EX31_RS03260 at 143.943 kb on + strand, within EX31_RS03260 at 143.987 kb on + strand, within EX31_RS03260 at 144.098 kb on + strand, within EX31_RS03260 at 144.098 kb on + strand, within EX31_RS03260 at 144.098 kb on + strand, within EX31_RS03260 at 144.159 kb on + strand, within EX31_RS03260 at 144.159 kb on + strand, within EX31_RS03260 at 144.159 kb on + strand, within EX31_RS03260 at 144.159 kb on + strand, within EX31_RS03260 at 144.162 kb on + strand, within EX31_RS03260 at 144.233 kb on + strand, within EX31_RS03260 at 144.233 kb on + strand, within EX31_RS03260 at 144.233 kb on + strand, within EX31_RS03260 at 144.233 kb on + strand, within EX31_RS03260 at 144.233 kb on + strand, within EX31_RS03260 at 144.233 kb on + strand, within EX31_RS03260 at 144.233 kb on + strand, within EX31_RS03260 at 144.236 kb on + strand, within EX31_RS03260 at 144.247 kb on + strand, within EX31_RS03260 at 144.247 kb on + strand, within EX31_RS03260 at 144.247 kb on + strand, within EX31_RS03260 at 144.247 kb on + strand, within EX31_RS03260 at 144.254 kb on + strand, within EX31_RS03260 at 144.323 kb on + strand, within EX31_RS03260 at 144.323 kb on + strand, within EX31_RS03260 at 144.323 kb on + strand, within EX31_RS03260 at 144.323 kb on + strand, within EX31_RS03260 at 144.323 kb on + strand, within EX31_RS03260 at 144.323 kb on + strand, within EX31_RS03260 at 144.339 kb on + strand, within EX31_RS03260 at 144.346 kb on + strand, within EX31_RS03260 at 144.352 kb on + strand, within EX31_RS03260 at 144.352 kb on + strand, within EX31_RS03260 at 144.475 kb on + strand at 144.475 kb on + strand at 145.016 kb on + strand at 145.016 kb on + strand at 145.017 kb on - strand at 145.070 kb on + strand at 145.090 kb on + strand at 145.090 kb on + strand at 145.090 kb on + strand at 145.090 kb on + strand at 145.090 kb on + strand at 145.090 kb on + strand at 145.091 kb on - strand at 145.108 kb on + strand at 145.108 kb on + strand at 145.109 kb on - strand at 145.109 kb on - strand at 145.109 kb on - strand at 145.160 kb on + strand at 145.444 kb on - strand, within EX31_RS03270 at 145.609 kb on + strand, within EX31_RS03270 at 145.609 kb on + strand, within EX31_RS03270 at 145.609 kb on + strand, within EX31_RS03270 at 145.609 kb on + strand, within EX31_RS03270 at 145.609 kb on + strand, within EX31_RS03270 at 145.610 kb on - strand, within EX31_RS03270 at 145.610 kb on - strand, within EX31_RS03270 at 145.610 kb on - strand, within EX31_RS03270 at 145.610 kb on - strand, within EX31_RS03270 at 145.653 kb on + strand, within EX31_RS03270 at 145.654 kb on - strand, within EX31_RS03270 at 145.687 kb on - strand, within EX31_RS03270 at 145.912 kb on - strand, within EX31_RS03270 at 145.984 kb on - strand, within EX31_RS03270
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N); RCH2 media; shaking remove 143,545 + EX31_RS03260 0.42 +1.3 143,643 + EX31_RS03260 0.47 -1.9 143,643 + EX31_RS03260 0.47 +0.6 143,643 + EX31_RS03260 0.47 +1.2 143,646 + EX31_RS03260 0.47 -0.5 143,668 + EX31_RS03260 0.49 -0.4 143,668 + EX31_RS03260 0.49 -0.1 143,668 + EX31_RS03260 0.49 -0.9 143,668 + EX31_RS03260 0.49 +0.9 143,669 - EX31_RS03260 0.49 -1.0 143,676 + EX31_RS03260 0.49 -1.3 143,676 + EX31_RS03260 0.49 -0.2 143,762 + EX31_RS03260 0.54 -0.3 143,762 + EX31_RS03260 0.54 -1.3 143,762 + EX31_RS03260 0.54 -0.5 143,777 - EX31_RS03260 0.55 +0.1 143,925 + EX31_RS03260 0.64 +0.5 143,927 + EX31_RS03260 0.64 -0.1 143,943 + EX31_RS03260 0.65 -0.3 143,987 + EX31_RS03260 0.67 -0.1 144,098 + EX31_RS03260 0.74 -0.5 144,098 + EX31_RS03260 0.74 +0.5 144,098 + EX31_RS03260 0.74 +1.3 144,159 + EX31_RS03260 0.78 -1.2 144,159 + EX31_RS03260 0.78 -0.7 144,159 + EX31_RS03260 0.78 -2.4 144,159 + EX31_RS03260 0.78 -0.9 144,162 + EX31_RS03260 0.78 -1.0 144,233 + EX31_RS03260 0.82 -0.5 144,233 + EX31_RS03260 0.82 +0.7 144,233 + EX31_RS03260 0.82 -4.0 144,233 + EX31_RS03260 0.82 -1.5 144,233 + EX31_RS03260 0.82 +0.5 144,233 + EX31_RS03260 0.82 -0.6 144,233 + EX31_RS03260 0.82 -0.1 144,236 + EX31_RS03260 0.82 -1.7 144,247 + EX31_RS03260 0.83 -1.6 144,247 + EX31_RS03260 0.83 -0.1 144,247 + EX31_RS03260 0.83 -0.7 144,247 + EX31_RS03260 0.83 -0.9 144,254 + EX31_RS03260 0.83 -1.4 144,323 + EX31_RS03260 0.87 -3.2 144,323 + EX31_RS03260 0.87 +1.5 144,323 + EX31_RS03260 0.87 -0.9 144,323 + EX31_RS03260 0.87 +0.1 144,323 + EX31_RS03260 0.87 +1.9 144,323 + EX31_RS03260 0.87 +0.0 144,339 + EX31_RS03260 0.88 -1.0 144,346 + EX31_RS03260 0.88 -0.0 144,352 + EX31_RS03260 0.89 +0.3 144,352 + EX31_RS03260 0.89 -0.1 144,475 + -1.7 144,475 + +0.5 145,016 + -0.2 145,016 + -0.2 145,017 - +0.1 145,070 + -0.1 145,090 + +0.6 145,090 + +0.1 145,090 + +1.1 145,090 + +0.4 145,090 + +0.1 145,090 + -0.2 145,091 - -0.7 145,108 + +0.5 145,108 + -0.5 145,109 - +0.5 145,109 - -0.3 145,109 - +1.1 145,160 + +0.5 145,444 - EX31_RS03270 0.30 +0.3 145,609 + EX31_RS03270 0.42 -0.1 145,609 + EX31_RS03270 0.42 -0.9 145,609 + EX31_RS03270 0.42 +2.1 145,609 + EX31_RS03270 0.42 +2.1 145,609 + EX31_RS03270 0.42 -2.0 145,610 - EX31_RS03270 0.42 +0.0 145,610 - EX31_RS03270 0.42 +0.1 145,610 - EX31_RS03270 0.42 +0.0 145,610 - EX31_RS03270 0.42 +0.0 145,653 + EX31_RS03270 0.45 -1.3 145,654 - EX31_RS03270 0.45 +0.1 145,687 - EX31_RS03270 0.48 +0.5 145,912 - EX31_RS03270 0.64 -1.2 145,984 - EX31_RS03270 0.69 -0.0
Or see this region's nucleotide sequence