Experiment: Ammonium chloride (N); RCH2 media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS03255 and EX31_RS03260 are separated by 132 nucleotides EX31_RS03260 and EX31_RS03265 overlap by 4 nucleotides EX31_RS03265 and EX31_RS03270 are separated by 5 nucleotides
EX31_RS03255: EX31_RS03255 - tRNA glutamyl-Q(34) synthetase GluQRS, at 141,828 to 142,703
_RS03255
EX31_RS03260: EX31_RS03260 - polynucleotide adenylyltransferase PcnB, at 142,836 to 144,542
_RS03260
EX31_RS03265: EX31_RS03265 - 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine diphosphokinase, at 144,539 to 145,018
_RS03265
EX31_RS03270: EX31_RS03270 - two-component system sensor histidine kinase QseC, at 145,024 to 146,409
_RS03270
Position (kb) 142
143
144
145 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 141.846 kb on + strand at 141.846 kb on + strand at 141.846 kb on + strand at 141.846 kb on + strand at 141.846 kb on + strand at 141.846 kb on + strand at 141.846 kb on + strand at 141.846 kb on + strand at 141.846 kb on + strand at 141.846 kb on + strand at 141.847 kb on - strand at 141.847 kb on - strand at 141.847 kb on - strand at 141.847 kb on - strand at 141.847 kb on - strand at 141.847 kb on - strand at 141.918 kb on + strand, within EX31_RS03255 at 141.918 kb on + strand, within EX31_RS03255 at 141.918 kb on + strand, within EX31_RS03255 at 141.918 kb on + strand, within EX31_RS03255 at 141.919 kb on - strand, within EX31_RS03255 at 141.919 kb on - strand, within EX31_RS03255 at 141.919 kb on - strand, within EX31_RS03255 at 141.952 kb on + strand, within EX31_RS03255 at 142.007 kb on + strand, within EX31_RS03255 at 142.107 kb on + strand, within EX31_RS03255 at 142.125 kb on + strand, within EX31_RS03255 at 142.125 kb on + strand, within EX31_RS03255 at 142.125 kb on + strand, within EX31_RS03255 at 142.126 kb on - strand, within EX31_RS03255 at 142.126 kb on - strand, within EX31_RS03255 at 142.126 kb on - strand, within EX31_RS03255 at 142.126 kb on - strand, within EX31_RS03255 at 142.126 kb on - strand, within EX31_RS03255 at 142.128 kb on + strand, within EX31_RS03255 at 142.128 kb on + strand, within EX31_RS03255 at 142.129 kb on - strand, within EX31_RS03255 at 142.129 kb on - strand, within EX31_RS03255 at 142.151 kb on + strand, within EX31_RS03255 at 142.151 kb on + strand, within EX31_RS03255 at 142.152 kb on - strand, within EX31_RS03255 at 142.152 kb on - strand, within EX31_RS03255 at 142.152 kb on - strand, within EX31_RS03255 at 142.173 kb on + strand, within EX31_RS03255 at 142.173 kb on + strand, within EX31_RS03255 at 142.173 kb on + strand, within EX31_RS03255 at 142.173 kb on + strand, within EX31_RS03255 at 142.173 kb on + strand, within EX31_RS03255 at 142.173 kb on + strand, within EX31_RS03255 at 142.173 kb on + strand, within EX31_RS03255 at 142.173 kb on + strand, within EX31_RS03255 at 142.173 kb on + strand, within EX31_RS03255 at 142.173 kb on + strand, within EX31_RS03255 at 142.174 kb on - strand, within EX31_RS03255 at 142.174 kb on - strand, within EX31_RS03255 at 142.174 kb on - strand, within EX31_RS03255 at 142.174 kb on - strand, within EX31_RS03255 at 142.222 kb on + strand, within EX31_RS03255 at 142.222 kb on + strand, within EX31_RS03255 at 142.222 kb on + strand, within EX31_RS03255 at 142.222 kb on + strand, within EX31_RS03255 at 142.222 kb on + strand, within EX31_RS03255 at 142.222 kb on + strand, within EX31_RS03255 at 142.222 kb on + strand, within EX31_RS03255 at 142.223 kb on - strand, within EX31_RS03255 at 142.223 kb on - strand, within EX31_RS03255 at 142.223 kb on - strand, within EX31_RS03255 at 142.223 kb on - strand, within EX31_RS03255 at 142.223 kb on - strand, within EX31_RS03255 at 142.223 kb on - strand, within EX31_RS03255 at 142.223 kb on - strand, within EX31_RS03255 at 142.223 kb on - strand, within EX31_RS03255 at 142.223 kb on - strand, within EX31_RS03255 at 142.279 kb on + strand, within EX31_RS03255 at 142.279 kb on + strand, within EX31_RS03255 at 142.280 kb on - strand, within EX31_RS03255 at 142.313 kb on + strand, within EX31_RS03255 at 142.313 kb on + strand, within EX31_RS03255 at 142.313 kb on + strand, within EX31_RS03255 at 142.314 kb on - strand, within EX31_RS03255 at 142.314 kb on - strand, within EX31_RS03255 at 142.316 kb on + strand, within EX31_RS03255 at 142.344 kb on + strand, within EX31_RS03255 at 142.344 kb on + strand, within EX31_RS03255 at 142.344 kb on + strand, within EX31_RS03255 at 142.344 kb on + strand, within EX31_RS03255 at 142.344 kb on + strand, within EX31_RS03255 at 142.344 kb on + strand, within EX31_RS03255 at 142.344 kb on + strand, within EX31_RS03255 at 142.344 kb on + strand, within EX31_RS03255 at 142.344 kb on + strand, within EX31_RS03255 at 142.344 kb on + strand, within EX31_RS03255 at 142.345 kb on - strand, within EX31_RS03255 at 142.347 kb on - strand, within EX31_RS03255 at 142.347 kb on - strand, within EX31_RS03255 at 142.357 kb on + strand, within EX31_RS03255 at 142.358 kb on - strand, within EX31_RS03255 at 142.358 kb on - strand, within EX31_RS03255 at 142.358 kb on - strand, within EX31_RS03255 at 142.444 kb on - strand, within EX31_RS03255 at 142.568 kb on + strand, within EX31_RS03255 at 142.568 kb on + strand, within EX31_RS03255 at 142.568 kb on + strand, within EX31_RS03255 at 142.569 kb on - strand, within EX31_RS03255 at 142.575 kb on - strand, within EX31_RS03255 at 142.623 kb on + strand at 142.623 kb on + strand at 142.623 kb on + strand at 142.624 kb on - strand at 142.624 kb on - strand at 142.625 kb on + strand at 142.625 kb on + strand at 142.625 kb on + strand at 142.625 kb on + strand at 142.626 kb on - strand at 142.626 kb on - strand at 142.636 kb on + strand at 142.636 kb on + strand at 142.636 kb on + strand at 142.637 kb on - strand at 142.639 kb on + strand at 142.639 kb on + strand at 142.640 kb on - strand at 142.642 kb on + strand at 142.642 kb on + strand at 142.642 kb on + strand at 142.643 kb on - strand at 142.744 kb on + strand at 142.744 kb on + strand at 142.744 kb on + strand at 142.744 kb on + strand at 142.744 kb on + strand at 142.744 kb on + strand at 142.744 kb on + strand at 142.744 kb on + strand at 142.745 kb on - strand at 142.745 kb on - strand at 142.745 kb on - strand at 142.745 kb on - strand at 142.745 kb on - strand at 142.832 kb on + strand at 142.840 kb on + strand at 142.840 kb on + strand at 142.840 kb on + strand at 142.841 kb on - strand at 142.853 kb on + strand at 142.910 kb on + strand at 143.108 kb on + strand, within EX31_RS03260 at 143.108 kb on + strand, within EX31_RS03260 at 143.137 kb on + strand, within EX31_RS03260 at 143.137 kb on + strand, within EX31_RS03260 at 143.137 kb on + strand, within EX31_RS03260 at 143.170 kb on + strand, within EX31_RS03260 at 143.170 kb on + strand, within EX31_RS03260 at 143.170 kb on + strand, within EX31_RS03260 at 143.170 kb on + strand, within EX31_RS03260 at 143.225 kb on + strand, within EX31_RS03260 at 143.225 kb on + strand, within EX31_RS03260 at 143.246 kb on + strand, within EX31_RS03260 at 143.246 kb on + strand, within EX31_RS03260 at 143.289 kb on + strand, within EX31_RS03260 at 143.289 kb on + strand, within EX31_RS03260 at 143.289 kb on + strand, within EX31_RS03260 at 143.393 kb on + strand, within EX31_RS03260 at 143.537 kb on + strand, within EX31_RS03260 at 143.545 kb on + strand, within EX31_RS03260 at 143.643 kb on + strand, within EX31_RS03260 at 143.643 kb on + strand, within EX31_RS03260 at 143.643 kb on + strand, within EX31_RS03260 at 143.646 kb on + strand, within EX31_RS03260 at 143.668 kb on + strand, within EX31_RS03260 at 143.668 kb on + strand, within EX31_RS03260 at 143.668 kb on + strand, within EX31_RS03260 at 143.668 kb on + strand, within EX31_RS03260 at 143.669 kb on - strand, within EX31_RS03260 at 143.676 kb on + strand, within EX31_RS03260 at 143.676 kb on + strand, within EX31_RS03260 at 143.762 kb on + strand, within EX31_RS03260 at 143.762 kb on + strand, within EX31_RS03260 at 143.762 kb on + strand, within EX31_RS03260 at 143.777 kb on - strand, within EX31_RS03260 at 143.925 kb on + strand, within EX31_RS03260 at 143.927 kb on + strand, within EX31_RS03260 at 143.943 kb on + strand, within EX31_RS03260 at 143.987 kb on + strand, within EX31_RS03260 at 144.098 kb on + strand, within EX31_RS03260 at 144.098 kb on + strand, within EX31_RS03260 at 144.098 kb on + strand, within EX31_RS03260 at 144.159 kb on + strand, within EX31_RS03260 at 144.159 kb on + strand, within EX31_RS03260 at 144.159 kb on + strand, within EX31_RS03260 at 144.159 kb on + strand, within EX31_RS03260 at 144.162 kb on + strand, within EX31_RS03260 at 144.233 kb on + strand, within EX31_RS03260 at 144.233 kb on + strand, within EX31_RS03260 at 144.233 kb on + strand, within EX31_RS03260 at 144.233 kb on + strand, within EX31_RS03260 at 144.233 kb on + strand, within EX31_RS03260 at 144.233 kb on + strand, within EX31_RS03260 at 144.233 kb on + strand, within EX31_RS03260 at 144.236 kb on + strand, within EX31_RS03260 at 144.247 kb on + strand, within EX31_RS03260 at 144.247 kb on + strand, within EX31_RS03260 at 144.247 kb on + strand, within EX31_RS03260 at 144.247 kb on + strand, within EX31_RS03260 at 144.254 kb on + strand, within EX31_RS03260 at 144.323 kb on + strand, within EX31_RS03260 at 144.323 kb on + strand, within EX31_RS03260 at 144.323 kb on + strand, within EX31_RS03260 at 144.323 kb on + strand, within EX31_RS03260 at 144.323 kb on + strand, within EX31_RS03260 at 144.323 kb on + strand, within EX31_RS03260 at 144.339 kb on + strand, within EX31_RS03260 at 144.346 kb on + strand, within EX31_RS03260 at 144.352 kb on + strand, within EX31_RS03260 at 144.352 kb on + strand, within EX31_RS03260 at 144.475 kb on + strand at 144.475 kb on + strand at 145.016 kb on + strand at 145.016 kb on + strand at 145.017 kb on - strand at 145.070 kb on + strand at 145.090 kb on + strand at 145.090 kb on + strand at 145.090 kb on + strand at 145.090 kb on + strand at 145.090 kb on + strand at 145.090 kb on + strand at 145.091 kb on - strand at 145.108 kb on + strand at 145.108 kb on + strand at 145.109 kb on - strand at 145.109 kb on - strand at 145.109 kb on - strand at 145.160 kb on + strand at 145.444 kb on - strand, within EX31_RS03270
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N); RCH2 media; shaking remove 141,846 + +1.4 141,846 + -0.0 141,846 + +0.4 141,846 + -0.2 141,846 + -0.3 141,846 + -0.4 141,846 + +1.4 141,846 + +0.2 141,846 + +0.8 141,846 + -0.0 141,847 - +0.9 141,847 - +0.6 141,847 - +2.4 141,847 - +0.5 141,847 - +0.4 141,847 - +1.3 141,918 + EX31_RS03255 0.10 -0.8 141,918 + EX31_RS03255 0.10 -0.3 141,918 + EX31_RS03255 0.10 +0.7 141,918 + EX31_RS03255 0.10 +0.3 141,919 - EX31_RS03255 0.10 +0.6 141,919 - EX31_RS03255 0.10 -0.6 141,919 - EX31_RS03255 0.10 +0.5 141,952 + EX31_RS03255 0.14 -0.6 142,007 + EX31_RS03255 0.20 -0.0 142,107 + EX31_RS03255 0.32 +2.2 142,125 + EX31_RS03255 0.34 +0.6 142,125 + EX31_RS03255 0.34 -1.0 142,125 + EX31_RS03255 0.34 -2.4 142,126 - EX31_RS03255 0.34 -0.0 142,126 - EX31_RS03255 0.34 +0.5 142,126 - EX31_RS03255 0.34 +0.6 142,126 - EX31_RS03255 0.34 -0.3 142,126 - EX31_RS03255 0.34 -0.5 142,128 + EX31_RS03255 0.34 -1.0 142,128 + EX31_RS03255 0.34 -0.7 142,129 - EX31_RS03255 0.34 +1.1 142,129 - EX31_RS03255 0.34 -0.8 142,151 + EX31_RS03255 0.37 +1.3 142,151 + EX31_RS03255 0.37 +0.9 142,152 - EX31_RS03255 0.37 -0.5 142,152 - EX31_RS03255 0.37 -3.0 142,152 - EX31_RS03255 0.37 -1.0 142,173 + EX31_RS03255 0.39 -1.0 142,173 + EX31_RS03255 0.39 +0.0 142,173 + EX31_RS03255 0.39 +0.4 142,173 + EX31_RS03255 0.39 +0.7 142,173 + EX31_RS03255 0.39 -0.3 142,173 + EX31_RS03255 0.39 -0.7 142,173 + EX31_RS03255 0.39 -0.2 142,173 + EX31_RS03255 0.39 -0.5 142,173 + EX31_RS03255 0.39 +0.1 142,173 + EX31_RS03255 0.39 +0.5 142,174 - EX31_RS03255 0.39 -0.5 142,174 - EX31_RS03255 0.39 -0.9 142,174 - EX31_RS03255 0.39 -0.3 142,174 - EX31_RS03255 0.39 -0.0 142,222 + EX31_RS03255 0.45 -0.2 142,222 + EX31_RS03255 0.45 +1.8 142,222 + EX31_RS03255 0.45 -0.3 142,222 + EX31_RS03255 0.45 +0.1 142,222 + EX31_RS03255 0.45 +0.1 142,222 + EX31_RS03255 0.45 +0.5 142,222 + EX31_RS03255 0.45 +0.5 142,223 - EX31_RS03255 0.45 +1.3 142,223 - EX31_RS03255 0.45 -0.2 142,223 - EX31_RS03255 0.45 +0.4 142,223 - EX31_RS03255 0.45 -0.4 142,223 - EX31_RS03255 0.45 +0.7 142,223 - EX31_RS03255 0.45 -0.3 142,223 - EX31_RS03255 0.45 -0.7 142,223 - EX31_RS03255 0.45 -0.5 142,223 - EX31_RS03255 0.45 +0.6 142,279 + EX31_RS03255 0.51 -0.0 142,279 + EX31_RS03255 0.51 -0.7 142,280 - EX31_RS03255 0.52 +0.4 142,313 + EX31_RS03255 0.55 -2.3 142,313 + EX31_RS03255 0.55 +0.3 142,313 + EX31_RS03255 0.55 -0.2 142,314 - EX31_RS03255 0.55 -0.1 142,314 - EX31_RS03255 0.55 -0.3 142,316 + EX31_RS03255 0.56 -1.1 142,344 + EX31_RS03255 0.59 +1.1 142,344 + EX31_RS03255 0.59 +1.1 142,344 + EX31_RS03255 0.59 -0.0 142,344 + EX31_RS03255 0.59 +0.7 142,344 + EX31_RS03255 0.59 -1.1 142,344 + EX31_RS03255 0.59 -0.9 142,344 + EX31_RS03255 0.59 +0.7 142,344 + EX31_RS03255 0.59 +1.2 142,344 + EX31_RS03255 0.59 +2.1 142,344 + EX31_RS03255 0.59 -0.6 142,345 - EX31_RS03255 0.59 -1.0 142,347 - EX31_RS03255 0.59 -1.5 142,347 - EX31_RS03255 0.59 +0.3 142,357 + EX31_RS03255 0.60 +0.2 142,358 - EX31_RS03255 0.61 +0.2 142,358 - EX31_RS03255 0.61 +0.7 142,358 - EX31_RS03255 0.61 +0.4 142,444 - EX31_RS03255 0.70 +0.7 142,568 + EX31_RS03255 0.84 +1.4 142,568 + EX31_RS03255 0.84 -0.2 142,568 + EX31_RS03255 0.84 +0.7 142,569 - EX31_RS03255 0.85 +0.2 142,575 - EX31_RS03255 0.85 +0.3 142,623 + -0.1 142,623 + -0.1 142,623 + +0.1 142,624 - +1.3 142,624 - +0.4 142,625 + +0.9 142,625 + -0.3 142,625 + +0.8 142,625 + +0.5 142,626 - +0.5 142,626 - +0.1 142,636 + +1.3 142,636 + +1.7 142,636 + +0.3 142,637 - -1.4 142,639 + -0.7 142,639 + -1.5 142,640 - -0.9 142,642 + +0.3 142,642 + -1.6 142,642 + -0.4 142,643 - +1.8 142,744 + -0.8 142,744 + -0.0 142,744 + +0.3 142,744 + +0.3 142,744 + +1.7 142,744 + +0.1 142,744 + +1.1 142,744 + -0.2 142,745 - -2.0 142,745 - -2.1 142,745 - +0.5 142,745 - -0.3 142,745 - -0.5 142,832 + -2.0 142,840 + -3.0 142,840 + -1.2 142,840 + +0.2 142,841 - -1.6 142,853 + +0.4 142,910 + +0.2 143,108 + EX31_RS03260 0.16 -1.6 143,108 + EX31_RS03260 0.16 +0.0 143,137 + EX31_RS03260 0.18 -0.8 143,137 + EX31_RS03260 0.18 -1.5 143,137 + EX31_RS03260 0.18 -0.2 143,170 + EX31_RS03260 0.20 -0.2 143,170 + EX31_RS03260 0.20 -0.5 143,170 + EX31_RS03260 0.20 -1.0 143,170 + EX31_RS03260 0.20 +0.1 143,225 + EX31_RS03260 0.23 -0.9 143,225 + EX31_RS03260 0.23 -3.1 143,246 + EX31_RS03260 0.24 -1.6 143,246 + EX31_RS03260 0.24 -1.7 143,289 + EX31_RS03260 0.27 +0.8 143,289 + EX31_RS03260 0.27 -0.0 143,289 + EX31_RS03260 0.27 +0.4 143,393 + EX31_RS03260 0.33 -0.7 143,537 + EX31_RS03260 0.41 -0.6 143,545 + EX31_RS03260 0.42 +1.3 143,643 + EX31_RS03260 0.47 -1.9 143,643 + EX31_RS03260 0.47 +0.6 143,643 + EX31_RS03260 0.47 +1.2 143,646 + EX31_RS03260 0.47 -0.5 143,668 + EX31_RS03260 0.49 -0.4 143,668 + EX31_RS03260 0.49 -0.1 143,668 + EX31_RS03260 0.49 -0.9 143,668 + EX31_RS03260 0.49 +0.9 143,669 - EX31_RS03260 0.49 -1.0 143,676 + EX31_RS03260 0.49 -1.3 143,676 + EX31_RS03260 0.49 -0.2 143,762 + EX31_RS03260 0.54 -0.3 143,762 + EX31_RS03260 0.54 -1.3 143,762 + EX31_RS03260 0.54 -0.5 143,777 - EX31_RS03260 0.55 +0.1 143,925 + EX31_RS03260 0.64 +0.5 143,927 + EX31_RS03260 0.64 -0.1 143,943 + EX31_RS03260 0.65 -0.3 143,987 + EX31_RS03260 0.67 -0.1 144,098 + EX31_RS03260 0.74 -0.5 144,098 + EX31_RS03260 0.74 +0.5 144,098 + EX31_RS03260 0.74 +1.3 144,159 + EX31_RS03260 0.78 -1.2 144,159 + EX31_RS03260 0.78 -0.7 144,159 + EX31_RS03260 0.78 -2.4 144,159 + EX31_RS03260 0.78 -0.9 144,162 + EX31_RS03260 0.78 -1.0 144,233 + EX31_RS03260 0.82 -0.5 144,233 + EX31_RS03260 0.82 +0.7 144,233 + EX31_RS03260 0.82 -4.0 144,233 + EX31_RS03260 0.82 -1.5 144,233 + EX31_RS03260 0.82 +0.5 144,233 + EX31_RS03260 0.82 -0.6 144,233 + EX31_RS03260 0.82 -0.1 144,236 + EX31_RS03260 0.82 -1.7 144,247 + EX31_RS03260 0.83 -1.6 144,247 + EX31_RS03260 0.83 -0.1 144,247 + EX31_RS03260 0.83 -0.7 144,247 + EX31_RS03260 0.83 -0.9 144,254 + EX31_RS03260 0.83 -1.4 144,323 + EX31_RS03260 0.87 -3.2 144,323 + EX31_RS03260 0.87 +1.5 144,323 + EX31_RS03260 0.87 -0.9 144,323 + EX31_RS03260 0.87 +0.1 144,323 + EX31_RS03260 0.87 +1.9 144,323 + EX31_RS03260 0.87 +0.0 144,339 + EX31_RS03260 0.88 -1.0 144,346 + EX31_RS03260 0.88 -0.0 144,352 + EX31_RS03260 0.89 +0.3 144,352 + EX31_RS03260 0.89 -0.1 144,475 + -1.7 144,475 + +0.5 145,016 + -0.2 145,016 + -0.2 145,017 - +0.1 145,070 + -0.1 145,090 + +0.6 145,090 + +0.1 145,090 + +1.1 145,090 + +0.4 145,090 + +0.1 145,090 + -0.2 145,091 - -0.7 145,108 + +0.5 145,108 + -0.5 145,109 - +0.5 145,109 - -0.3 145,109 - +1.1 145,160 + +0.5 145,444 - EX31_RS03270 0.30 +0.3
Or see this region's nucleotide sequence