Experiment: Ammonium chloride (N); RCH2 media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS02680 and EX31_RS02685 are separated by 22 nucleotides EX31_RS02685 and EX31_RS02690 are separated by 72 nucleotides EX31_RS02690 and EX31_RS02695 are separated by 86 nucleotides EX31_RS02695 and EX31_RS02700 overlap by 16 nucleotides EX31_RS02700 and EX31_RS02705 are separated by 21 nucleotides
EX31_RS02680: EX31_RS02680 - type IV pilus twitching motility protein PilT, at 8,288 to 9,310
_RS02680
EX31_RS02685: EX31_RS02685 - YggS family pyridoxal phosphate-dependent enzyme, at 9,333 to 10,052
_RS02685
EX31_RS02690: EX31_RS02690 - pyrroline-5-carboxylate reductase, at 10,125 to 10,946
_RS02690
EX31_RS02695: EX31_RS02695 - YggT family protein, at 11,033 to 11,587
_RS02695
EX31_RS02700: EX31_RS02700 - YggU family protein, at 11,572 to 11,877
_RS02700
EX31_RS02705: EX31_RS02705 - XTP/dITP diphosphatase, at 11,899 to 12,507
_RS02705
Position (kb)
10
11 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 9.125 kb on - strand, within EX31_RS02680 at 9.125 kb on - strand, within EX31_RS02680 at 9.125 kb on - strand, within EX31_RS02680 at 9.125 kb on - strand, within EX31_RS02680 at 9.125 kb on - strand, within EX31_RS02680 at 9.125 kb on - strand, within EX31_RS02680 at 9.125 kb on - strand, within EX31_RS02680 at 9.125 kb on - strand, within EX31_RS02680 at 9.125 kb on - strand, within EX31_RS02680 at 9.125 kb on - strand, within EX31_RS02680 at 9.209 kb on + strand at 9.209 kb on + strand at 9.209 kb on + strand at 9.209 kb on + strand at 9.209 kb on + strand at 9.210 kb on - strand at 9.210 kb on - strand at 9.278 kb on + strand at 9.279 kb on - strand at 9.283 kb on + strand at 9.283 kb on + strand at 9.283 kb on + strand at 9.283 kb on + strand at 9.283 kb on + strand at 9.283 kb on + strand at 9.283 kb on + strand at 9.283 kb on + strand at 9.283 kb on + strand at 9.283 kb on + strand at 9.283 kb on + strand at 9.284 kb on - strand at 9.284 kb on - strand at 9.284 kb on - strand at 9.284 kb on - strand at 9.284 kb on - strand at 9.284 kb on - strand at 9.284 kb on - strand at 9.293 kb on + strand at 9.293 kb on + strand at 9.293 kb on + strand at 9.305 kb on - strand at 9.306 kb on + strand at 9.341 kb on + strand at 9.370 kb on + strand at 9.371 kb on - strand at 9.371 kb on - strand at 9.520 kb on - strand, within EX31_RS02685 at 9.520 kb on - strand, within EX31_RS02685 at 9.520 kb on - strand, within EX31_RS02685 at 9.608 kb on + strand, within EX31_RS02685 at 9.608 kb on + strand, within EX31_RS02685 at 9.609 kb on - strand, within EX31_RS02685 at 9.713 kb on - strand, within EX31_RS02685 at 9.752 kb on + strand, within EX31_RS02685 at 9.753 kb on - strand, within EX31_RS02685 at 9.820 kb on + strand, within EX31_RS02685 at 9.920 kb on + strand, within EX31_RS02685 at 9.921 kb on - strand, within EX31_RS02685 at 9.921 kb on - strand, within EX31_RS02685 at 9.921 kb on - strand, within EX31_RS02685 at 9.953 kb on + strand, within EX31_RS02685 at 9.953 kb on + strand, within EX31_RS02685 at 9.954 kb on - strand, within EX31_RS02685 at 9.965 kb on + strand, within EX31_RS02685 at 9.965 kb on + strand, within EX31_RS02685 at 9.965 kb on + strand, within EX31_RS02685 at 9.965 kb on + strand, within EX31_RS02685 at 9.965 kb on + strand, within EX31_RS02685 at 9.965 kb on + strand, within EX31_RS02685 at 9.966 kb on - strand, within EX31_RS02685 at 9.966 kb on - strand, within EX31_RS02685 at 9.966 kb on - strand, within EX31_RS02685 at 9.966 kb on - strand, within EX31_RS02685 at 10.035 kb on + strand at 10.036 kb on - strand at 10.036 kb on - strand at 10.042 kb on + strand at 10.042 kb on + strand at 10.042 kb on + strand at 10.043 kb on - strand at 10.043 kb on - strand at 10.043 kb on - strand at 10.043 kb on - strand at 10.050 kb on + strand at 10.057 kb on + strand at 10.057 kb on + strand at 10.058 kb on - strand at 10.058 kb on - strand at 10.069 kb on + strand at 10.069 kb on + strand at 10.077 kb on + strand at 10.078 kb on - strand at 10.086 kb on + strand at 10.107 kb on + strand at 10.178 kb on + strand at 10.265 kb on + strand, within EX31_RS02690 at 10.269 kb on + strand, within EX31_RS02690 at 10.269 kb on + strand, within EX31_RS02690 at 10.270 kb on - strand, within EX31_RS02690 at 10.270 kb on - strand, within EX31_RS02690 at 10.405 kb on + strand, within EX31_RS02690 at 10.563 kb on + strand, within EX31_RS02690 at 10.622 kb on + strand, within EX31_RS02690 at 10.622 kb on + strand, within EX31_RS02690 at 10.633 kb on + strand, within EX31_RS02690 at 10.633 kb on + strand, within EX31_RS02690 at 10.633 kb on + strand, within EX31_RS02690 at 10.634 kb on - strand, within EX31_RS02690 at 10.634 kb on - strand, within EX31_RS02690 at 10.634 kb on - strand, within EX31_RS02690 at 10.662 kb on + strand, within EX31_RS02690 at 10.771 kb on + strand, within EX31_RS02690 at 10.871 kb on + strand at 10.871 kb on + strand at 10.944 kb on + strand at 10.944 kb on + strand at 10.944 kb on + strand at 10.944 kb on + strand at 10.944 kb on + strand at 10.945 kb on - strand at 10.947 kb on + strand at 10.947 kb on + strand at 10.947 kb on + strand at 10.948 kb on - strand at 10.960 kb on + strand at 10.961 kb on - strand at 10.965 kb on + strand at 11.023 kb on + strand at 11.032 kb on + strand at 11.033 kb on - strand at 11.067 kb on + strand at 11.067 kb on + strand at 11.067 kb on + strand at 11.068 kb on - strand at 11.075 kb on + strand at 11.194 kb on + strand, within EX31_RS02695 at 11.278 kb on + strand, within EX31_RS02695 at 11.279 kb on - strand, within EX31_RS02695 at 11.331 kb on + strand, within EX31_RS02695 at 11.426 kb on + strand, within EX31_RS02695 at 11.473 kb on + strand, within EX31_RS02695 at 11.531 kb on + strand, within EX31_RS02695 at 11.531 kb on + strand, within EX31_RS02695 at 11.531 kb on + strand, within EX31_RS02695 at 11.531 kb on + strand, within EX31_RS02695 at 11.531 kb on + strand, within EX31_RS02695 at 11.532 kb on - strand at 11.533 kb on + strand at 11.533 kb on + strand at 11.533 kb on + strand at 11.543 kb on + strand at 11.543 kb on + strand at 11.543 kb on + strand at 11.632 kb on - strand, within EX31_RS02700 at 11.760 kb on + strand, within EX31_RS02700 at 11.811 kb on + strand, within EX31_RS02700 at 11.826 kb on + strand, within EX31_RS02700 at 11.827 kb on - strand, within EX31_RS02700 at 11.827 kb on - strand, within EX31_RS02700 at 11.827 kb on - strand, within EX31_RS02700 at 11.861 kb on + strand at 11.875 kb on + strand at 11.882 kb on + strand at 11.882 kb on + strand at 11.882 kb on + strand at 11.882 kb on + strand at 11.882 kb on + strand at 11.883 kb on - strand at 11.883 kb on - strand at 11.883 kb on - strand at 11.913 kb on - strand at 11.915 kb on + strand at 11.915 kb on + strand at 11.915 kb on + strand at 11.915 kb on + strand at 11.915 kb on + strand at 11.915 kb on + strand at 11.915 kb on + strand at 11.915 kb on + strand at 11.915 kb on + strand at 11.915 kb on + strand at 11.915 kb on + strand at 11.916 kb on - strand at 11.916 kb on - strand at 11.916 kb on - strand at 11.934 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N); RCH2 media; shaking remove 9,125 - EX31_RS02680 0.82 -0.3 9,125 - EX31_RS02680 0.82 -0.5 9,125 - EX31_RS02680 0.82 +0.1 9,125 - EX31_RS02680 0.82 +0.8 9,125 - EX31_RS02680 0.82 +0.5 9,125 - EX31_RS02680 0.82 +0.0 9,125 - EX31_RS02680 0.82 -0.3 9,125 - EX31_RS02680 0.82 -0.8 9,125 - EX31_RS02680 0.82 +1.0 9,125 - EX31_RS02680 0.82 +0.3 9,209 + +1.3 9,209 + +0.8 9,209 + +1.5 9,209 + -0.6 9,209 + -0.7 9,210 - +0.2 9,210 - -0.3 9,278 + +0.9 9,279 - +0.0 9,283 + +1.1 9,283 + +0.9 9,283 + +0.3 9,283 + +0.4 9,283 + +0.8 9,283 + -0.2 9,283 + +1.2 9,283 + +0.9 9,283 + +0.5 9,283 + +0.1 9,283 + +0.8 9,284 - +0.0 9,284 - -0.8 9,284 - -0.4 9,284 - +0.1 9,284 - +0.2 9,284 - +0.3 9,284 - +1.0 9,293 + +0.0 9,293 + -0.4 9,293 + -0.1 9,305 - -0.4 9,306 + +0.5 9,341 + +0.1 9,370 + -0.8 9,371 - +0.5 9,371 - +0.1 9,520 - EX31_RS02685 0.26 -0.6 9,520 - EX31_RS02685 0.26 +0.1 9,520 - EX31_RS02685 0.26 +0.2 9,608 + EX31_RS02685 0.38 +0.2 9,608 + EX31_RS02685 0.38 +0.5 9,609 - EX31_RS02685 0.38 +1.5 9,713 - EX31_RS02685 0.53 +0.4 9,752 + EX31_RS02685 0.58 -1.3 9,753 - EX31_RS02685 0.58 -0.5 9,820 + EX31_RS02685 0.68 -0.6 9,920 + EX31_RS02685 0.82 +0.0 9,921 - EX31_RS02685 0.82 +2.0 9,921 - EX31_RS02685 0.82 -0.0 9,921 - EX31_RS02685 0.82 -0.7 9,953 + EX31_RS02685 0.86 -0.1 9,953 + EX31_RS02685 0.86 -0.1 9,954 - EX31_RS02685 0.86 +0.2 9,965 + EX31_RS02685 0.88 -0.1 9,965 + EX31_RS02685 0.88 +0.7 9,965 + EX31_RS02685 0.88 +0.2 9,965 + EX31_RS02685 0.88 +1.0 9,965 + EX31_RS02685 0.88 +0.1 9,965 + EX31_RS02685 0.88 +0.5 9,966 - EX31_RS02685 0.88 +1.0 9,966 - EX31_RS02685 0.88 +0.7 9,966 - EX31_RS02685 0.88 +0.1 9,966 - EX31_RS02685 0.88 -0.0 10,035 + +0.2 10,036 - -1.8 10,036 - -0.1 10,042 + +0.1 10,042 + -0.1 10,042 + +1.2 10,043 - +0.7 10,043 - +0.0 10,043 - +1.5 10,043 - -2.8 10,050 + +1.0 10,057 + +1.4 10,057 + +1.1 10,058 - +1.3 10,058 - +1.3 10,069 + +0.5 10,069 + -0.8 10,077 + +2.1 10,078 - -0.1 10,086 + +0.5 10,107 + +0.3 10,178 + -1.9 10,265 + EX31_RS02690 0.17 -2.6 10,269 + EX31_RS02690 0.18 -1.5 10,269 + EX31_RS02690 0.18 -3.2 10,270 - EX31_RS02690 0.18 -1.2 10,270 - EX31_RS02690 0.18 -2.1 10,405 + EX31_RS02690 0.34 -3.2 10,563 + EX31_RS02690 0.53 -3.1 10,622 + EX31_RS02690 0.60 -2.1 10,622 + EX31_RS02690 0.60 -3.4 10,633 + EX31_RS02690 0.62 -0.7 10,633 + EX31_RS02690 0.62 -2.0 10,633 + EX31_RS02690 0.62 -2.2 10,634 - EX31_RS02690 0.62 -1.6 10,634 - EX31_RS02690 0.62 -1.8 10,634 - EX31_RS02690 0.62 -0.2 10,662 + EX31_RS02690 0.65 -3.0 10,771 + EX31_RS02690 0.79 -4.0 10,871 + -2.9 10,871 + -2.0 10,944 + -0.5 10,944 + +0.3 10,944 + +0.5 10,944 + -0.1 10,944 + -0.2 10,945 - +0.0 10,947 + +0.2 10,947 + -0.0 10,947 + +0.2 10,948 - +2.6 10,960 + -0.1 10,961 - +0.9 10,965 + -0.7 11,023 + +0.8 11,032 + +0.7 11,033 - +0.1 11,067 + -1.6 11,067 + +1.9 11,067 + +1.4 11,068 - -0.9 11,075 + +0.4 11,194 + EX31_RS02695 0.29 -2.7 11,278 + EX31_RS02695 0.44 +1.4 11,279 - EX31_RS02695 0.44 +0.1 11,331 + EX31_RS02695 0.54 -1.5 11,426 + EX31_RS02695 0.71 +0.0 11,473 + EX31_RS02695 0.79 +0.2 11,531 + EX31_RS02695 0.90 +0.1 11,531 + EX31_RS02695 0.90 +1.3 11,531 + EX31_RS02695 0.90 +0.3 11,531 + EX31_RS02695 0.90 +0.1 11,531 + EX31_RS02695 0.90 -1.3 11,532 - +1.3 11,533 + -0.3 11,533 + +1.3 11,533 + -0.5 11,543 + -0.8 11,543 + -0.5 11,543 + -0.3 11,632 - EX31_RS02700 0.20 +1.1 11,760 + EX31_RS02700 0.61 +0.2 11,811 + EX31_RS02700 0.78 +0.9 11,826 + EX31_RS02700 0.83 -1.4 11,827 - EX31_RS02700 0.83 -0.2 11,827 - EX31_RS02700 0.83 +1.4 11,827 - EX31_RS02700 0.83 -0.2 11,861 + -0.0 11,875 + -0.7 11,882 + +0.5 11,882 + +0.9 11,882 + -1.3 11,882 + +0.3 11,882 + -0.4 11,883 - +2.9 11,883 - -1.2 11,883 - -0.8 11,913 - -1.0 11,915 + -1.3 11,915 + -0.8 11,915 + +0.7 11,915 + +0.8 11,915 + +0.3 11,915 + -0.5 11,915 + +1.4 11,915 + +1.3 11,915 + -0.1 11,915 + +0.7 11,915 + -0.1 11,916 - -0.7 11,916 - -1.7 11,916 - +0.5 11,934 + -0.6
Or see this region's nucleotide sequence