Experiment: Ammonium chloride (N); RCH2 media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS02420 and EX31_RS02425 are separated by 9 nucleotides EX31_RS02425 and EX31_RS02430 are separated by 2 nucleotides EX31_RS02430 and EX31_RS02435 overlap by 4 nucleotides
EX31_RS02420: EX31_RS02420 - type III PLP-dependent enzyme, at 513,708 to 515,015
_RS02420
EX31_RS02425: EX31_RS02425 - hypothetical protein, at 515,025 to 515,936
_RS02425
EX31_RS02430: EX31_RS02430 - 3-oxoacyl-ACP synthase, at 515,939 to 516,961
_RS02430
EX31_RS02435: EX31_RS02435 - acyl--CoA ligase, at 516,958 to 518,538
_RS02435
Position (kb) 515
516
517 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 514.955 kb on + strand at 514.984 kb on + strand at 514.984 kb on + strand at 514.984 kb on + strand at 514.984 kb on + strand at 514.984 kb on + strand at 514.985 kb on - strand at 514.985 kb on - strand at 514.985 kb on - strand at 514.985 kb on - strand at 514.985 kb on - strand at 514.986 kb on + strand at 514.986 kb on + strand at 514.986 kb on + strand at 514.987 kb on - strand at 514.987 kb on - strand at 514.987 kb on - strand at 514.996 kb on + strand at 514.996 kb on + strand at 515.024 kb on + strand at 515.024 kb on + strand at 515.025 kb on - strand at 515.025 kb on - strand at 515.025 kb on - strand at 515.051 kb on + strand at 515.052 kb on - strand at 515.093 kb on + strand at 515.094 kb on - strand at 515.094 kb on - strand at 515.348 kb on + strand, within EX31_RS02425 at 515.352 kb on + strand, within EX31_RS02425 at 515.353 kb on - strand, within EX31_RS02425 at 515.382 kb on - strand, within EX31_RS02425 at 515.405 kb on - strand, within EX31_RS02425 at 515.477 kb on + strand, within EX31_RS02425 at 515.518 kb on - strand, within EX31_RS02425 at 515.530 kb on - strand, within EX31_RS02425 at 515.618 kb on + strand, within EX31_RS02425 at 515.618 kb on + strand, within EX31_RS02425 at 515.618 kb on + strand, within EX31_RS02425 at 515.618 kb on + strand, within EX31_RS02425 at 515.621 kb on + strand, within EX31_RS02425 at 515.621 kb on + strand, within EX31_RS02425 at 515.621 kb on + strand, within EX31_RS02425 at 515.621 kb on + strand, within EX31_RS02425 at 515.621 kb on + strand, within EX31_RS02425 at 515.622 kb on - strand, within EX31_RS02425 at 515.622 kb on - strand, within EX31_RS02425 at 515.673 kb on + strand, within EX31_RS02425 at 515.673 kb on + strand, within EX31_RS02425 at 515.673 kb on + strand, within EX31_RS02425 at 515.674 kb on - strand, within EX31_RS02425 at 515.674 kb on - strand, within EX31_RS02425 at 515.732 kb on + strand, within EX31_RS02425 at 515.733 kb on - strand, within EX31_RS02425 at 515.818 kb on + strand, within EX31_RS02425 at 515.819 kb on - strand, within EX31_RS02425 at 515.819 kb on - strand, within EX31_RS02425 at 515.843 kb on - strand, within EX31_RS02425 at 515.845 kb on + strand at 515.867 kb on + strand at 515.868 kb on - strand at 515.868 kb on - strand at 515.878 kb on + strand at 515.878 kb on + strand at 515.878 kb on + strand at 515.878 kb on + strand at 515.879 kb on - strand at 515.879 kb on - strand at 515.879 kb on - strand at 515.881 kb on + strand at 515.882 kb on - strand at 515.882 kb on - strand at 515.882 kb on - strand at 515.903 kb on + strand at 515.903 kb on + strand at 515.903 kb on + strand at 515.903 kb on + strand at 515.903 kb on + strand at 515.904 kb on - strand at 515.942 kb on + strand at 515.942 kb on + strand at 515.942 kb on + strand at 515.942 kb on + strand at 515.942 kb on + strand at 515.942 kb on + strand at 515.942 kb on + strand at 515.942 kb on + strand at 515.942 kb on + strand at 515.942 kb on + strand at 515.942 kb on + strand at 515.942 kb on + strand at 515.943 kb on - strand at 515.943 kb on - strand at 515.943 kb on - strand at 515.945 kb on - strand at 515.945 kb on - strand at 515.967 kb on + strand at 515.968 kb on - strand at 515.968 kb on - strand at 516.053 kb on - strand, within EX31_RS02430 at 516.147 kb on + strand, within EX31_RS02430 at 516.147 kb on + strand, within EX31_RS02430 at 516.147 kb on + strand, within EX31_RS02430 at 516.148 kb on - strand, within EX31_RS02430 at 516.164 kb on + strand, within EX31_RS02430 at 516.231 kb on + strand, within EX31_RS02430 at 516.232 kb on - strand, within EX31_RS02430 at 516.458 kb on + strand, within EX31_RS02430 at 516.458 kb on + strand, within EX31_RS02430 at 516.458 kb on + strand, within EX31_RS02430 at 516.458 kb on + strand, within EX31_RS02430 at 516.458 kb on + strand, within EX31_RS02430 at 516.458 kb on + strand, within EX31_RS02430 at 516.458 kb on + strand, within EX31_RS02430 at 516.459 kb on - strand, within EX31_RS02430 at 516.459 kb on - strand, within EX31_RS02430 at 516.459 kb on - strand, within EX31_RS02430 at 516.459 kb on - strand, within EX31_RS02430 at 516.459 kb on - strand, within EX31_RS02430 at 516.549 kb on + strand, within EX31_RS02430 at 516.617 kb on + strand, within EX31_RS02430 at 516.777 kb on + strand, within EX31_RS02430 at 516.778 kb on - strand, within EX31_RS02430 at 516.975 kb on - strand at 516.975 kb on - strand at 517.046 kb on + strand at 517.047 kb on - strand at 517.110 kb on + strand at 517.183 kb on + strand, within EX31_RS02435 at 517.391 kb on + strand, within EX31_RS02435 at 517.392 kb on - strand, within EX31_RS02435 at 517.471 kb on + strand, within EX31_RS02435 at 517.471 kb on + strand, within EX31_RS02435 at 517.525 kb on - strand, within EX31_RS02435 at 517.525 kb on - strand, within EX31_RS02435 at 517.525 kb on - strand, within EX31_RS02435 at 517.525 kb on - strand, within EX31_RS02435 at 517.525 kb on - strand, within EX31_RS02435 at 517.555 kb on + strand, within EX31_RS02435 at 517.555 kb on + strand, within EX31_RS02435 at 517.556 kb on - strand, within EX31_RS02435 at 517.556 kb on - strand, within EX31_RS02435 at 517.801 kb on - strand, within EX31_RS02435 at 517.813 kb on - strand, within EX31_RS02435 at 517.884 kb on + strand, within EX31_RS02435 at 517.888 kb on + strand, within EX31_RS02435 at 517.888 kb on + strand, within EX31_RS02435 at 517.888 kb on + strand, within EX31_RS02435 at 517.889 kb on - strand, within EX31_RS02435 at 517.947 kb on + strand, within EX31_RS02435 at 517.947 kb on + strand, within EX31_RS02435 at 517.947 kb on + strand, within EX31_RS02435 at 517.948 kb on - strand, within EX31_RS02435 at 517.948 kb on - strand, within EX31_RS02435 at 517.948 kb on - strand, within EX31_RS02435
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N); RCH2 media; shaking remove 514,955 + +1.0 514,984 + -1.4 514,984 + +1.4 514,984 + +0.6 514,984 + +0.5 514,984 + -0.5 514,985 - -1.0 514,985 - +1.5 514,985 - -0.6 514,985 - -1.2 514,985 - +1.8 514,986 + -1.4 514,986 + +1.2 514,986 + +0.2 514,987 - +1.6 514,987 - +0.9 514,987 - +1.2 514,996 + +0.5 514,996 + +1.8 515,024 + +0.0 515,024 + +0.4 515,025 - +0.0 515,025 - -1.1 515,025 - -0.1 515,051 + +0.2 515,052 - +0.5 515,093 + -0.5 515,094 - -0.5 515,094 - -0.2 515,348 + EX31_RS02425 0.35 +0.4 515,352 + EX31_RS02425 0.36 -0.1 515,353 - EX31_RS02425 0.36 +0.7 515,382 - EX31_RS02425 0.39 +0.2 515,405 - EX31_RS02425 0.42 +2.4 515,477 + EX31_RS02425 0.50 +1.0 515,518 - EX31_RS02425 0.54 +0.2 515,530 - EX31_RS02425 0.55 +0.7 515,618 + EX31_RS02425 0.65 +0.5 515,618 + EX31_RS02425 0.65 +0.3 515,618 + EX31_RS02425 0.65 -1.3 515,618 + EX31_RS02425 0.65 +0.1 515,621 + EX31_RS02425 0.65 -1.3 515,621 + EX31_RS02425 0.65 +2.0 515,621 + EX31_RS02425 0.65 +0.2 515,621 + EX31_RS02425 0.65 -0.3 515,621 + EX31_RS02425 0.65 -0.8 515,622 - EX31_RS02425 0.65 +0.8 515,622 - EX31_RS02425 0.65 +0.2 515,673 + EX31_RS02425 0.71 -1.2 515,673 + EX31_RS02425 0.71 +1.9 515,673 + EX31_RS02425 0.71 +0.5 515,674 - EX31_RS02425 0.71 +0.8 515,674 - EX31_RS02425 0.71 -0.5 515,732 + EX31_RS02425 0.78 +0.8 515,733 - EX31_RS02425 0.78 -0.5 515,818 + EX31_RS02425 0.87 +1.2 515,819 - EX31_RS02425 0.87 +0.6 515,819 - EX31_RS02425 0.87 -0.0 515,843 - EX31_RS02425 0.90 +0.4 515,845 + +1.5 515,867 + +0.4 515,868 - +0.5 515,868 - -0.8 515,878 + -0.3 515,878 + -0.4 515,878 + +1.2 515,878 + -0.3 515,879 - -1.8 515,879 - +0.0 515,879 - +0.5 515,881 + -3.5 515,882 - +0.9 515,882 - +0.6 515,882 - -0.7 515,903 + +0.7 515,903 + -0.1 515,903 + +0.7 515,903 + +1.8 515,903 + +0.4 515,904 - -0.1 515,942 + +0.8 515,942 + -0.4 515,942 + +0.6 515,942 + +0.8 515,942 + +0.6 515,942 + +1.3 515,942 + -1.0 515,942 + -0.8 515,942 + -0.4 515,942 + -1.4 515,942 + +0.4 515,942 + +2.1 515,943 - -0.3 515,943 - -0.2 515,943 - +0.7 515,945 - -0.4 515,945 - -0.2 515,967 + +0.4 515,968 - +0.2 515,968 - -1.8 516,053 - EX31_RS02430 0.11 -1.8 516,147 + EX31_RS02430 0.20 +0.1 516,147 + EX31_RS02430 0.20 -0.2 516,147 + EX31_RS02430 0.20 -0.7 516,148 - EX31_RS02430 0.20 +0.4 516,164 + EX31_RS02430 0.22 -1.4 516,231 + EX31_RS02430 0.29 +0.0 516,232 - EX31_RS02430 0.29 +0.8 516,458 + EX31_RS02430 0.51 -0.9 516,458 + EX31_RS02430 0.51 -0.8 516,458 + EX31_RS02430 0.51 -2.1 516,458 + EX31_RS02430 0.51 -1.0 516,458 + EX31_RS02430 0.51 +0.5 516,458 + EX31_RS02430 0.51 +0.2 516,458 + EX31_RS02430 0.51 -0.9 516,459 - EX31_RS02430 0.51 +1.4 516,459 - EX31_RS02430 0.51 -2.2 516,459 - EX31_RS02430 0.51 -1.2 516,459 - EX31_RS02430 0.51 -0.5 516,459 - EX31_RS02430 0.51 +1.1 516,549 + EX31_RS02430 0.60 +0.6 516,617 + EX31_RS02430 0.66 -0.4 516,777 + EX31_RS02430 0.82 -0.1 516,778 - EX31_RS02430 0.82 -0.6 516,975 - -1.1 516,975 - -0.4 517,046 + -0.1 517,047 - +0.2 517,110 + +1.2 517,183 + EX31_RS02435 0.14 -0.2 517,391 + EX31_RS02435 0.27 -0.4 517,392 - EX31_RS02435 0.27 -1.8 517,471 + EX31_RS02435 0.32 +0.4 517,471 + EX31_RS02435 0.32 +0.4 517,525 - EX31_RS02435 0.36 +0.3 517,525 - EX31_RS02435 0.36 +0.0 517,525 - EX31_RS02435 0.36 -0.4 517,525 - EX31_RS02435 0.36 +0.7 517,525 - EX31_RS02435 0.36 -1.9 517,555 + EX31_RS02435 0.38 +0.1 517,555 + EX31_RS02435 0.38 -1.0 517,556 - EX31_RS02435 0.38 +0.5 517,556 - EX31_RS02435 0.38 +0.2 517,801 - EX31_RS02435 0.53 -0.9 517,813 - EX31_RS02435 0.54 -0.6 517,884 + EX31_RS02435 0.59 +0.8 517,888 + EX31_RS02435 0.59 +1.4 517,888 + EX31_RS02435 0.59 +1.8 517,888 + EX31_RS02435 0.59 +1.7 517,889 - EX31_RS02435 0.59 +0.8 517,947 + EX31_RS02435 0.63 +1.4 517,947 + EX31_RS02435 0.63 +1.1 517,947 + EX31_RS02435 0.63 +1.0 517,948 - EX31_RS02435 0.63 +1.9 517,948 - EX31_RS02435 0.63 +0.6 517,948 - EX31_RS02435 0.63 +3.0
Or see this region's nucleotide sequence