Experiment: Ammonium chloride (N); RCH2 media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS02230 and EX31_RS02235 are separated by 120 nucleotides EX31_RS02235 and EX31_RS02240 are separated by 118 nucleotides EX31_RS02240 and EX31_RS02245 are separated by 14 nucleotides
EX31_RS02230: EX31_RS02230 - SDR family oxidoreductase, at 482,645 to 483,502
_RS02230
EX31_RS02235: EX31_RS02235 - helix-turn-helix transcriptional regulator, at 483,623 to 484,039
_RS02235
EX31_RS02240: EX31_RS02240 - antibiotic biosynthesis monooxygenase, at 484,158 to 484,724
_RS02240
EX31_RS02245: EX31_RS02245 - amidohydrolase, at 484,739 to 486,619
_RS02245
Position (kb) 483
484
485 Strain fitness (log2 ratio) -2
-1
0
1
2 at 482.634 kb on + strand at 482.720 kb on - strand at 482.720 kb on - strand at 482.776 kb on + strand, within EX31_RS02230 at 482.776 kb on + strand, within EX31_RS02230 at 482.777 kb on - strand, within EX31_RS02230 at 482.777 kb on - strand, within EX31_RS02230 at 482.846 kb on - strand, within EX31_RS02230 at 482.846 kb on - strand, within EX31_RS02230 at 482.847 kb on + strand, within EX31_RS02230 at 482.848 kb on - strand, within EX31_RS02230 at 482.983 kb on - strand, within EX31_RS02230 at 483.072 kb on + strand, within EX31_RS02230 at 483.073 kb on - strand, within EX31_RS02230 at 483.073 kb on - strand, within EX31_RS02230 at 483.178 kb on + strand, within EX31_RS02230 at 483.179 kb on - strand, within EX31_RS02230 at 483.521 kb on + strand at 483.537 kb on + strand at 483.537 kb on + strand at 483.538 kb on - strand at 483.579 kb on - strand at 483.586 kb on - strand at 483.605 kb on - strand at 483.605 kb on - strand at 483.619 kb on + strand at 483.630 kb on + strand at 483.703 kb on + strand, within EX31_RS02235 at 483.703 kb on + strand, within EX31_RS02235 at 483.754 kb on - strand, within EX31_RS02235 at 483.849 kb on + strand, within EX31_RS02235 at 483.849 kb on + strand, within EX31_RS02235 at 483.917 kb on + strand, within EX31_RS02235 at 484.026 kb on + strand at 484.037 kb on + strand at 484.037 kb on + strand at 484.038 kb on - strand at 484.038 kb on - strand at 484.038 kb on - strand at 484.038 kb on - strand at 484.042 kb on - strand at 484.054 kb on + strand at 484.080 kb on - strand at 484.080 kb on - strand at 484.091 kb on + strand at 484.091 kb on + strand at 484.103 kb on - strand at 484.135 kb on - strand at 484.135 kb on - strand at 484.157 kb on + strand at 484.157 kb on + strand at 484.157 kb on + strand at 484.157 kb on + strand at 484.157 kb on + strand at 484.157 kb on + strand at 484.157 kb on + strand at 484.157 kb on + strand at 484.157 kb on + strand at 484.157 kb on + strand at 484.158 kb on - strand at 484.158 kb on - strand at 484.158 kb on - strand at 484.158 kb on - strand at 484.158 kb on - strand at 484.158 kb on - strand at 484.158 kb on - strand at 484.158 kb on - strand at 484.158 kb on - strand at 484.158 kb on - strand at 484.158 kb on - strand at 484.158 kb on - strand at 484.158 kb on - strand at 484.158 kb on - strand at 484.158 kb on - strand at 484.158 kb on - strand at 484.158 kb on - strand at 484.208 kb on + strand at 484.237 kb on - strand, within EX31_RS02240 at 484.237 kb on - strand, within EX31_RS02240 at 484.260 kb on - strand, within EX31_RS02240 at 484.274 kb on + strand, within EX31_RS02240 at 484.332 kb on + strand, within EX31_RS02240 at 484.332 kb on + strand, within EX31_RS02240 at 484.332 kb on + strand, within EX31_RS02240 at 484.332 kb on + strand, within EX31_RS02240 at 484.333 kb on - strand, within EX31_RS02240 at 484.333 kb on - strand, within EX31_RS02240 at 484.333 kb on - strand, within EX31_RS02240 at 484.334 kb on + strand, within EX31_RS02240 at 484.335 kb on - strand, within EX31_RS02240 at 484.335 kb on - strand, within EX31_RS02240 at 484.335 kb on - strand, within EX31_RS02240 at 484.335 kb on - strand, within EX31_RS02240 at 484.335 kb on - strand, within EX31_RS02240 at 484.335 kb on - strand, within EX31_RS02240 at 484.335 kb on - strand, within EX31_RS02240 at 484.335 kb on - strand, within EX31_RS02240 at 484.370 kb on + strand, within EX31_RS02240 at 484.371 kb on - strand, within EX31_RS02240 at 484.397 kb on + strand, within EX31_RS02240 at 484.524 kb on + strand, within EX31_RS02240 at 484.524 kb on + strand, within EX31_RS02240 at 484.524 kb on + strand, within EX31_RS02240 at 484.524 kb on + strand, within EX31_RS02240 at 484.524 kb on + strand, within EX31_RS02240 at 484.524 kb on + strand, within EX31_RS02240 at 484.525 kb on - strand, within EX31_RS02240 at 484.525 kb on - strand, within EX31_RS02240 at 484.525 kb on - strand, within EX31_RS02240 at 484.525 kb on - strand, within EX31_RS02240 at 484.525 kb on - strand, within EX31_RS02240 at 484.982 kb on + strand, within EX31_RS02245 at 485.035 kb on + strand, within EX31_RS02245 at 485.035 kb on + strand, within EX31_RS02245 at 485.036 kb on - strand, within EX31_RS02245 at 485.036 kb on - strand, within EX31_RS02245
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N); RCH2 media; shaking remove 482,634 + -1.4 482,720 - +0.5 482,720 - +1.1 482,776 + EX31_RS02230 0.15 +0.5 482,776 + EX31_RS02230 0.15 -0.3 482,777 - EX31_RS02230 0.15 +1.3 482,777 - EX31_RS02230 0.15 -1.8 482,846 - EX31_RS02230 0.23 +0.8 482,846 - EX31_RS02230 0.23 -0.8 482,847 + EX31_RS02230 0.24 +0.8 482,848 - EX31_RS02230 0.24 -0.2 482,983 - EX31_RS02230 0.39 -0.6 483,072 + EX31_RS02230 0.50 -0.5 483,073 - EX31_RS02230 0.50 +0.4 483,073 - EX31_RS02230 0.50 -1.2 483,178 + EX31_RS02230 0.62 -0.5 483,179 - EX31_RS02230 0.62 +0.9 483,521 + -1.1 483,537 + +0.6 483,537 + +0.4 483,538 - -0.1 483,579 - -0.0 483,586 - -1.4 483,605 - -1.6 483,605 - +1.8 483,619 + +1.0 483,630 + +0.8 483,703 + EX31_RS02235 0.19 -1.5 483,703 + EX31_RS02235 0.19 -0.3 483,754 - EX31_RS02235 0.31 -1.0 483,849 + EX31_RS02235 0.54 -1.1 483,849 + EX31_RS02235 0.54 +0.4 483,917 + EX31_RS02235 0.71 -1.3 484,026 + +0.1 484,037 + +0.3 484,037 + -0.1 484,038 - +0.0 484,038 - +1.7 484,038 - -1.4 484,038 - -2.3 484,042 - -0.3 484,054 + -0.2 484,080 - +0.5 484,080 - +0.3 484,091 + -0.1 484,091 + +0.0 484,103 - -1.6 484,135 - -1.1 484,135 - -0.3 484,157 + -0.8 484,157 + +0.7 484,157 + +0.8 484,157 + +1.1 484,157 + +0.4 484,157 + +0.2 484,157 + +2.3 484,157 + -1.0 484,157 + -1.6 484,157 + +0.2 484,158 - +1.3 484,158 - +0.4 484,158 - -0.0 484,158 - +0.4 484,158 - +0.4 484,158 - +0.5 484,158 - -0.6 484,158 - +0.4 484,158 - -0.3 484,158 - -0.2 484,158 - -0.2 484,158 - +0.0 484,158 - +0.1 484,158 - +1.3 484,158 - +0.2 484,158 - +0.2 484,158 - +0.4 484,208 + -0.5 484,237 - EX31_RS02240 0.14 -0.2 484,237 - EX31_RS02240 0.14 +0.1 484,260 - EX31_RS02240 0.18 -1.1 484,274 + EX31_RS02240 0.20 -0.2 484,332 + EX31_RS02240 0.31 -0.8 484,332 + EX31_RS02240 0.31 -0.9 484,332 + EX31_RS02240 0.31 -2.2 484,332 + EX31_RS02240 0.31 -2.4 484,333 - EX31_RS02240 0.31 -0.4 484,333 - EX31_RS02240 0.31 +0.3 484,333 - EX31_RS02240 0.31 +0.6 484,334 + EX31_RS02240 0.31 +0.2 484,335 - EX31_RS02240 0.31 +1.4 484,335 - EX31_RS02240 0.31 +1.4 484,335 - EX31_RS02240 0.31 +0.2 484,335 - EX31_RS02240 0.31 -0.6 484,335 - EX31_RS02240 0.31 +1.0 484,335 - EX31_RS02240 0.31 +1.2 484,335 - EX31_RS02240 0.31 -2.6 484,335 - EX31_RS02240 0.31 +0.2 484,370 + EX31_RS02240 0.37 +0.4 484,371 - EX31_RS02240 0.38 +0.9 484,397 + EX31_RS02240 0.42 +0.2 484,524 + EX31_RS02240 0.65 +0.2 484,524 + EX31_RS02240 0.65 -0.8 484,524 + EX31_RS02240 0.65 +0.8 484,524 + EX31_RS02240 0.65 -1.8 484,524 + EX31_RS02240 0.65 -1.9 484,524 + EX31_RS02240 0.65 -0.4 484,525 - EX31_RS02240 0.65 +1.3 484,525 - EX31_RS02240 0.65 -1.2 484,525 - EX31_RS02240 0.65 +1.8 484,525 - EX31_RS02240 0.65 +0.1 484,525 - EX31_RS02240 0.65 +0.4 484,982 + EX31_RS02245 0.13 -1.9 485,035 + EX31_RS02245 0.16 +0.5 485,035 + EX31_RS02245 0.16 -0.0 485,036 - EX31_RS02245 0.16 -1.4 485,036 - EX31_RS02245 0.16 +1.1
Or see this region's nucleotide sequence