Experiment: Ammonium chloride (N); RCH2 media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS01220 and EX31_RS01225 are separated by 69 nucleotides EX31_RS01225 and EX31_RS01230 are separated by 51 nucleotides
EX31_RS01220: EX31_RS01220 - peptide MFS transporter, at 271,213 to 272,766
_RS01220
EX31_RS01225: EX31_RS01225 - glutaredoxin-dependent arsenate reductase, at 272,836 to 273,267
_RS01225
EX31_RS01230: EX31_RS01230 - arsenic transporter, at 273,319 to 274,608
_RS01230
Position (kb)
272
273
274 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 271.843 kb on + strand, within EX31_RS01220 at 271.890 kb on + strand, within EX31_RS01220 at 272.011 kb on + strand, within EX31_RS01220 at 272.012 kb on - strand, within EX31_RS01220 at 272.043 kb on + strand, within EX31_RS01220 at 272.044 kb on - strand, within EX31_RS01220 at 272.047 kb on + strand, within EX31_RS01220 at 272.047 kb on + strand, within EX31_RS01220 at 272.185 kb on + strand, within EX31_RS01220 at 272.186 kb on - strand, within EX31_RS01220 at 272.253 kb on + strand, within EX31_RS01220 at 272.423 kb on + strand, within EX31_RS01220 at 272.606 kb on + strand, within EX31_RS01220 at 272.606 kb on + strand, within EX31_RS01220 at 272.606 kb on + strand, within EX31_RS01220 at 272.606 kb on + strand, within EX31_RS01220 at 272.607 kb on - strand, within EX31_RS01220 at 272.607 kb on - strand, within EX31_RS01220 at 272.625 kb on + strand at 272.626 kb on - strand at 272.733 kb on + strand at 272.734 kb on - strand at 272.848 kb on + strand at 272.851 kb on - strand at 272.851 kb on - strand at 272.851 kb on - strand at 272.851 kb on - strand at 272.933 kb on - strand, within EX31_RS01225 at 272.933 kb on - strand, within EX31_RS01225 at 272.933 kb on - strand, within EX31_RS01225 at 272.933 kb on - strand, within EX31_RS01225 at 272.953 kb on + strand, within EX31_RS01225 at 273.071 kb on + strand, within EX31_RS01225 at 273.259 kb on - strand at 273.291 kb on + strand at 273.292 kb on - strand at 273.321 kb on - strand at 273.404 kb on + strand at 273.404 kb on + strand at 273.408 kb on - strand at 273.408 kb on - strand at 273.426 kb on + strand at 273.426 kb on + strand at 273.426 kb on + strand at 273.455 kb on - strand, within EX31_RS01230 at 273.521 kb on + strand, within EX31_RS01230 at 273.522 kb on - strand, within EX31_RS01230 at 273.522 kb on - strand, within EX31_RS01230 at 273.568 kb on - strand, within EX31_RS01230 at 273.598 kb on - strand, within EX31_RS01230 at 273.649 kb on - strand, within EX31_RS01230 at 273.658 kb on + strand, within EX31_RS01230 at 273.658 kb on + strand, within EX31_RS01230 at 273.729 kb on - strand, within EX31_RS01230 at 273.740 kb on + strand, within EX31_RS01230 at 273.740 kb on + strand, within EX31_RS01230 at 273.740 kb on + strand, within EX31_RS01230 at 273.740 kb on + strand, within EX31_RS01230 at 273.741 kb on - strand, within EX31_RS01230 at 273.741 kb on - strand, within EX31_RS01230 at 273.741 kb on - strand, within EX31_RS01230 at 273.741 kb on - strand, within EX31_RS01230 at 273.801 kb on + strand, within EX31_RS01230 at 273.802 kb on - strand, within EX31_RS01230 at 273.828 kb on + strand, within EX31_RS01230 at 273.828 kb on + strand, within EX31_RS01230 at 273.829 kb on - strand, within EX31_RS01230 at 273.829 kb on - strand, within EX31_RS01230 at 273.829 kb on - strand, within EX31_RS01230 at 273.829 kb on - strand, within EX31_RS01230 at 273.830 kb on + strand, within EX31_RS01230 at 273.831 kb on - strand, within EX31_RS01230 at 273.831 kb on - strand, within EX31_RS01230 at 273.988 kb on - strand, within EX31_RS01230 at 273.992 kb on + strand, within EX31_RS01230 at 273.992 kb on + strand, within EX31_RS01230 at 274.125 kb on + strand, within EX31_RS01230 at 274.125 kb on + strand, within EX31_RS01230 at 274.199 kb on - strand, within EX31_RS01230
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N); RCH2 media; shaking remove 271,843 + EX31_RS01220 0.41 -0.0 271,890 + EX31_RS01220 0.44 +0.8 272,011 + EX31_RS01220 0.51 -0.8 272,012 - EX31_RS01220 0.51 +0.8 272,043 + EX31_RS01220 0.53 +0.3 272,044 - EX31_RS01220 0.53 +2.1 272,047 + EX31_RS01220 0.54 -1.7 272,047 + EX31_RS01220 0.54 +0.2 272,185 + EX31_RS01220 0.63 +0.7 272,186 - EX31_RS01220 0.63 +0.4 272,253 + EX31_RS01220 0.67 -0.4 272,423 + EX31_RS01220 0.78 -0.6 272,606 + EX31_RS01220 0.90 +0.2 272,606 + EX31_RS01220 0.90 +1.3 272,606 + EX31_RS01220 0.90 +1.0 272,606 + EX31_RS01220 0.90 +1.1 272,607 - EX31_RS01220 0.90 -1.4 272,607 - EX31_RS01220 0.90 -1.2 272,625 + +1.0 272,626 - -0.1 272,733 + -1.9 272,734 - +0.9 272,848 + -0.9 272,851 - +1.8 272,851 - -0.8 272,851 - +0.8 272,851 - +2.1 272,933 - EX31_RS01225 0.22 -1.8 272,933 - EX31_RS01225 0.22 -0.4 272,933 - EX31_RS01225 0.22 -0.9 272,933 - EX31_RS01225 0.22 -0.3 272,953 + EX31_RS01225 0.27 -1.5 273,071 + EX31_RS01225 0.54 +1.1 273,259 - -1.8 273,291 + +0.6 273,292 - +0.8 273,321 - -0.1 273,404 + +0.5 273,404 + +0.1 273,408 - +0.7 273,408 - -0.2 273,426 + -1.5 273,426 + +0.8 273,426 + +0.7 273,455 - EX31_RS01230 0.11 -0.1 273,521 + EX31_RS01230 0.16 -1.5 273,522 - EX31_RS01230 0.16 +0.8 273,522 - EX31_RS01230 0.16 +0.6 273,568 - EX31_RS01230 0.19 +1.8 273,598 - EX31_RS01230 0.22 +0.9 273,649 - EX31_RS01230 0.26 -0.8 273,658 + EX31_RS01230 0.26 +0.5 273,658 + EX31_RS01230 0.26 +2.1 273,729 - EX31_RS01230 0.32 +1.1 273,740 + EX31_RS01230 0.33 +1.1 273,740 + EX31_RS01230 0.33 +0.6 273,740 + EX31_RS01230 0.33 +1.3 273,740 + EX31_RS01230 0.33 -0.2 273,741 - EX31_RS01230 0.33 -0.5 273,741 - EX31_RS01230 0.33 -0.6 273,741 - EX31_RS01230 0.33 +1.1 273,741 - EX31_RS01230 0.33 +0.5 273,801 + EX31_RS01230 0.37 +0.2 273,802 - EX31_RS01230 0.37 +0.9 273,828 + EX31_RS01230 0.39 -0.7 273,828 + EX31_RS01230 0.39 +0.1 273,829 - EX31_RS01230 0.40 -0.7 273,829 - EX31_RS01230 0.40 -2.3 273,829 - EX31_RS01230 0.40 +0.4 273,829 - EX31_RS01230 0.40 +0.3 273,830 + EX31_RS01230 0.40 -0.2 273,831 - EX31_RS01230 0.40 -0.2 273,831 - EX31_RS01230 0.40 +1.1 273,988 - EX31_RS01230 0.52 +0.5 273,992 + EX31_RS01230 0.52 +0.6 273,992 + EX31_RS01230 0.52 +0.1 274,125 + EX31_RS01230 0.62 +1.6 274,125 + EX31_RS01230 0.62 +1.1 274,199 - EX31_RS01230 0.68 +0.9
Or see this region's nucleotide sequence