Experiment: R2A with Polymyxin B sulfate 0.016 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS20545 and CA265_RS20550 are separated by 200 nucleotides CA265_RS20550 and CA265_RS20555 are separated by 138 nucleotides CA265_RS20555 and CA265_RS20560 are separated by 47 nucleotides
CA265_RS20545: CA265_RS20545 - 2-amino-3-ketobutyrate CoA ligase, at 5,083,191 to 5,084,453
_RS20545
CA265_RS20550: CA265_RS20550 - hypothetical protein, at 5,084,654 to 5,085,283
_RS20550
CA265_RS20555: CA265_RS20555 - galactose-1-epimerase, at 5,085,422 to 5,086,513
_RS20555
CA265_RS20560: CA265_RS20560 - hypothetical protein, at 5,086,561 to 5,087,649
_RS20560
Position (kb) 5085
5086
5087 Strain fitness (log2 ratio) -2
-1
0
1 at 5084.424 kb on - strand at 5084.482 kb on + strand at 5084.569 kb on + strand at 5084.569 kb on + strand at 5084.569 kb on + strand at 5084.757 kb on + strand, within CA265_RS20550 at 5084.784 kb on + strand, within CA265_RS20550 at 5084.907 kb on - strand, within CA265_RS20550 at 5084.944 kb on + strand, within CA265_RS20550 at 5084.945 kb on - strand, within CA265_RS20550 at 5084.974 kb on + strand, within CA265_RS20550 at 5084.989 kb on + strand, within CA265_RS20550 at 5085.070 kb on - strand, within CA265_RS20550 at 5085.092 kb on - strand, within CA265_RS20550 at 5085.153 kb on + strand, within CA265_RS20550 at 5085.163 kb on - strand, within CA265_RS20550 at 5085.197 kb on - strand, within CA265_RS20550 at 5085.206 kb on + strand, within CA265_RS20550 at 5085.271 kb on + strand at 5085.271 kb on + strand at 5085.283 kb on + strand at 5085.284 kb on - strand at 5085.284 kb on - strand at 5085.314 kb on + strand at 5085.327 kb on + strand at 5085.403 kb on + strand at 5085.444 kb on + strand at 5085.715 kb on + strand, within CA265_RS20555 at 5085.715 kb on + strand, within CA265_RS20555 at 5085.812 kb on + strand, within CA265_RS20555 at 5085.975 kb on + strand, within CA265_RS20555 at 5085.980 kb on + strand, within CA265_RS20555 at 5086.009 kb on + strand, within CA265_RS20555 at 5086.076 kb on + strand, within CA265_RS20555 at 5086.165 kb on + strand, within CA265_RS20555 at 5086.171 kb on + strand, within CA265_RS20555 at 5086.677 kb on + strand, within CA265_RS20560 at 5086.704 kb on + strand, within CA265_RS20560 at 5086.803 kb on + strand, within CA265_RS20560 at 5086.803 kb on + strand, within CA265_RS20560 at 5086.803 kb on + strand, within CA265_RS20560 at 5086.803 kb on + strand, within CA265_RS20560 at 5086.889 kb on + strand, within CA265_RS20560 at 5087.153 kb on + strand, within CA265_RS20560 at 5087.153 kb on + strand, within CA265_RS20560 at 5087.156 kb on + strand, within CA265_RS20560 at 5087.162 kb on + strand, within CA265_RS20560 at 5087.218 kb on + strand, within CA265_RS20560 at 5087.218 kb on + strand, within CA265_RS20560 at 5087.218 kb on + strand, within CA265_RS20560 at 5087.257 kb on + strand, within CA265_RS20560 at 5087.292 kb on + strand, within CA265_RS20560 at 5087.350 kb on + strand, within CA265_RS20560 at 5087.350 kb on + strand, within CA265_RS20560 at 5087.392 kb on + strand, within CA265_RS20560 at 5087.402 kb on + strand, within CA265_RS20560 at 5087.402 kb on + strand, within CA265_RS20560 at 5087.464 kb on + strand, within CA265_RS20560
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.016 mg/ml remove 5,084,424 - -1.1 5,084,482 + +0.1 5,084,569 + -0.2 5,084,569 + -0.4 5,084,569 + +0.1 5,084,757 + CA265_RS20550 0.16 +1.6 5,084,784 + CA265_RS20550 0.21 -1.9 5,084,907 - CA265_RS20550 0.40 -1.6 5,084,944 + CA265_RS20550 0.46 -2.0 5,084,945 - CA265_RS20550 0.46 -0.1 5,084,974 + CA265_RS20550 0.51 -1.5 5,084,989 + CA265_RS20550 0.53 -1.3 5,085,070 - CA265_RS20550 0.66 -1.8 5,085,092 - CA265_RS20550 0.70 +0.3 5,085,153 + CA265_RS20550 0.79 -0.0 5,085,163 - CA265_RS20550 0.81 +1.6 5,085,197 - CA265_RS20550 0.86 -0.3 5,085,206 + CA265_RS20550 0.88 -0.6 5,085,271 + -1.0 5,085,271 + +1.3 5,085,283 + -0.6 5,085,284 - -1.0 5,085,284 - -1.7 5,085,314 + -0.1 5,085,327 + +0.6 5,085,403 + -0.3 5,085,444 + +0.2 5,085,715 + CA265_RS20555 0.27 +0.8 5,085,715 + CA265_RS20555 0.27 -0.3 5,085,812 + CA265_RS20555 0.36 -0.3 5,085,975 + CA265_RS20555 0.51 -1.1 5,085,980 + CA265_RS20555 0.51 +0.3 5,086,009 + CA265_RS20555 0.54 +0.4 5,086,076 + CA265_RS20555 0.60 +0.9 5,086,165 + CA265_RS20555 0.68 -0.7 5,086,171 + CA265_RS20555 0.69 +0.1 5,086,677 + CA265_RS20560 0.11 +0.9 5,086,704 + CA265_RS20560 0.13 +0.6 5,086,803 + CA265_RS20560 0.22 +0.9 5,086,803 + CA265_RS20560 0.22 +0.4 5,086,803 + CA265_RS20560 0.22 +0.2 5,086,803 + CA265_RS20560 0.22 -0.1 5,086,889 + CA265_RS20560 0.30 +0.4 5,087,153 + CA265_RS20560 0.54 +0.5 5,087,153 + CA265_RS20560 0.54 +0.4 5,087,156 + CA265_RS20560 0.55 +0.7 5,087,162 + CA265_RS20560 0.55 -0.1 5,087,218 + CA265_RS20560 0.60 -0.3 5,087,218 + CA265_RS20560 0.60 +0.6 5,087,218 + CA265_RS20560 0.60 +0.9 5,087,257 + CA265_RS20560 0.64 -1.0 5,087,292 + CA265_RS20560 0.67 -0.4 5,087,350 + CA265_RS20560 0.72 -1.6 5,087,350 + CA265_RS20560 0.72 -0.7 5,087,392 + CA265_RS20560 0.76 -0.1 5,087,402 + CA265_RS20560 0.77 -0.4 5,087,402 + CA265_RS20560 0.77 +0.3 5,087,464 + CA265_RS20560 0.83 -1.0
Or see this region's nucleotide sequence