Experiment: R2A with Polymyxin B sulfate 0.016 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS20015 and CA265_RS20020 are separated by 222 nucleotides CA265_RS20020 and CA265_RS20025 are separated by 113 nucleotides
CA265_RS20015: CA265_RS20015 - glutamine--tRNA ligase, at 4,948,209 to 4,949,864
_RS20015
CA265_RS20020: CA265_RS20020 - N-acetylmuramoyl-L-alanine amidase, at 4,950,087 to 4,950,923
_RS20020
CA265_RS20025: CA265_RS20025 - Zn-dependent hydrolase, at 4,951,037 to 4,952,716
_RS20025
Position (kb)
4950
4951 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4949.887 kb on + strand at 4949.888 kb on - strand at 4949.888 kb on - strand at 4949.918 kb on - strand at 4950.006 kb on + strand at 4950.006 kb on + strand at 4950.107 kb on + strand at 4950.108 kb on - strand at 4950.110 kb on - strand at 4950.149 kb on + strand at 4950.220 kb on + strand, within CA265_RS20020 at 4950.277 kb on + strand, within CA265_RS20020 at 4950.391 kb on + strand, within CA265_RS20020 at 4950.392 kb on - strand, within CA265_RS20020 at 4950.524 kb on + strand, within CA265_RS20020 at 4950.528 kb on + strand, within CA265_RS20020 at 4950.528 kb on + strand, within CA265_RS20020 at 4950.553 kb on + strand, within CA265_RS20020 at 4950.554 kb on - strand, within CA265_RS20020 at 4950.554 kb on - strand, within CA265_RS20020 at 4950.567 kb on - strand, within CA265_RS20020 at 4950.567 kb on - strand, within CA265_RS20020 at 4950.573 kb on + strand, within CA265_RS20020 at 4950.574 kb on - strand, within CA265_RS20020 at 4950.578 kb on + strand, within CA265_RS20020 at 4950.599 kb on - strand, within CA265_RS20020 at 4950.599 kb on - strand, within CA265_RS20020 at 4950.656 kb on + strand, within CA265_RS20020 at 4950.695 kb on + strand, within CA265_RS20020 at 4950.700 kb on - strand, within CA265_RS20020 at 4950.755 kb on - strand, within CA265_RS20020 at 4950.818 kb on - strand, within CA265_RS20020 at 4950.820 kb on - strand, within CA265_RS20020 at 4950.867 kb on - strand at 4950.867 kb on - strand at 4950.890 kb on + strand at 4950.895 kb on - strand at 4950.923 kb on - strand at 4951.017 kb on + strand at 4951.026 kb on + strand at 4951.046 kb on - strand at 4951.121 kb on - strand at 4951.164 kb on + strand at 4951.206 kb on + strand, within CA265_RS20025 at 4951.222 kb on + strand, within CA265_RS20025 at 4951.222 kb on + strand, within CA265_RS20025 at 4951.223 kb on - strand, within CA265_RS20025 at 4951.223 kb on - strand, within CA265_RS20025 at 4951.233 kb on + strand, within CA265_RS20025 at 4951.233 kb on + strand, within CA265_RS20025 at 4951.233 kb on + strand, within CA265_RS20025 at 4951.234 kb on - strand, within CA265_RS20025 at 4951.299 kb on - strand, within CA265_RS20025 at 4951.375 kb on + strand, within CA265_RS20025 at 4951.401 kb on + strand, within CA265_RS20025 at 4951.412 kb on - strand, within CA265_RS20025 at 4951.523 kb on + strand, within CA265_RS20025 at 4951.579 kb on - strand, within CA265_RS20025 at 4951.582 kb on - strand, within CA265_RS20025 at 4951.753 kb on + strand, within CA265_RS20025 at 4951.753 kb on + strand, within CA265_RS20025 at 4951.783 kb on + strand, within CA265_RS20025 at 4951.792 kb on + strand, within CA265_RS20025 at 4951.793 kb on - strand, within CA265_RS20025 at 4951.863 kb on - strand, within CA265_RS20025
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.016 mg/ml remove 4,949,887 + +2.2 4,949,888 - +0.3 4,949,888 - -0.6 4,949,918 - +0.2 4,950,006 + +0.2 4,950,006 + +0.6 4,950,107 + +0.0 4,950,108 - -0.5 4,950,110 - -1.3 4,950,149 + -0.1 4,950,220 + CA265_RS20020 0.16 +0.5 4,950,277 + CA265_RS20020 0.23 +1.0 4,950,391 + CA265_RS20020 0.36 +0.4 4,950,392 - CA265_RS20020 0.36 -0.5 4,950,524 + CA265_RS20020 0.52 -0.4 4,950,528 + CA265_RS20020 0.53 -0.2 4,950,528 + CA265_RS20020 0.53 +1.8 4,950,553 + CA265_RS20020 0.56 +0.1 4,950,554 - CA265_RS20020 0.56 +0.8 4,950,554 - CA265_RS20020 0.56 -0.4 4,950,567 - CA265_RS20020 0.57 -2.1 4,950,567 - CA265_RS20020 0.57 -0.6 4,950,573 + CA265_RS20020 0.58 +0.9 4,950,574 - CA265_RS20020 0.58 +1.4 4,950,578 + CA265_RS20020 0.59 +0.5 4,950,599 - CA265_RS20020 0.61 +2.4 4,950,599 - CA265_RS20020 0.61 -0.3 4,950,656 + CA265_RS20020 0.68 -1.3 4,950,695 + CA265_RS20020 0.73 -0.5 4,950,700 - CA265_RS20020 0.73 -0.1 4,950,755 - CA265_RS20020 0.80 -2.1 4,950,818 - CA265_RS20020 0.87 -0.6 4,950,820 - CA265_RS20020 0.88 -2.5 4,950,867 - +1.0 4,950,867 - +0.4 4,950,890 + -1.3 4,950,895 - -0.4 4,950,923 - -0.7 4,951,017 + +1.1 4,951,026 + +1.7 4,951,046 - +0.1 4,951,121 - +0.6 4,951,164 + +1.6 4,951,206 + CA265_RS20025 0.10 +0.2 4,951,222 + CA265_RS20025 0.11 +0.6 4,951,222 + CA265_RS20025 0.11 -0.1 4,951,223 - CA265_RS20025 0.11 +0.1 4,951,223 - CA265_RS20025 0.11 -0.1 4,951,233 + CA265_RS20025 0.12 -0.1 4,951,233 + CA265_RS20025 0.12 +0.1 4,951,233 + CA265_RS20025 0.12 +0.8 4,951,234 - CA265_RS20025 0.12 +1.2 4,951,299 - CA265_RS20025 0.16 +1.1 4,951,375 + CA265_RS20025 0.20 -0.1 4,951,401 + CA265_RS20025 0.22 +1.0 4,951,412 - CA265_RS20025 0.22 +0.3 4,951,523 + CA265_RS20025 0.29 +1.2 4,951,579 - CA265_RS20025 0.32 -0.7 4,951,582 - CA265_RS20025 0.32 -0.2 4,951,753 + CA265_RS20025 0.43 +0.3 4,951,753 + CA265_RS20025 0.43 -0.3 4,951,783 + CA265_RS20025 0.44 -0.1 4,951,792 + CA265_RS20025 0.45 +0.6 4,951,793 - CA265_RS20025 0.45 -0.4 4,951,863 - CA265_RS20025 0.49 +0.8
Or see this region's nucleotide sequence