Experiment: R2A with Polymyxin B sulfate 0.016 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS02055 and CA265_RS02060 are separated by 30 nucleotides CA265_RS02060 and CA265_RS02065 are separated by 37 nucleotides CA265_RS02065 and CA265_RS02070 are separated by 170 nucleotides
CA265_RS02055: CA265_RS02055 - DNA ligase (NAD(+)) LigA, at 519,277 to 521,292
_RS02055
CA265_RS02060: CA265_RS02060 - hypothetical protein, at 521,323 to 522,825
_RS02060
CA265_RS02065: CA265_RS02065 - hypothetical protein, at 522,863 to 523,537
_RS02065
CA265_RS02070: CA265_RS02070 - tyrosine--tRNA ligase, at 523,708 to 524,994
_RS02070
Position (kb) 521
522
523 Strain fitness (log2 ratio) -2
-1
0
1 at 521.302 kb on - strand at 521.304 kb on - strand at 521.395 kb on - strand at 521.398 kb on - strand at 521.442 kb on - strand at 521.517 kb on - strand, within CA265_RS02060 at 521.517 kb on - strand, within CA265_RS02060 at 521.518 kb on + strand, within CA265_RS02060 at 521.518 kb on + strand, within CA265_RS02060 at 521.519 kb on - strand, within CA265_RS02060 at 521.519 kb on - strand, within CA265_RS02060 at 521.519 kb on - strand, within CA265_RS02060 at 521.519 kb on - strand, within CA265_RS02060 at 521.519 kb on - strand, within CA265_RS02060 at 521.535 kb on + strand, within CA265_RS02060 at 521.619 kb on + strand, within CA265_RS02060 at 521.619 kb on + strand, within CA265_RS02060 at 521.620 kb on - strand, within CA265_RS02060 at 521.620 kb on - strand at 521.620 kb on - strand, within CA265_RS02060 at 521.620 kb on - strand, within CA265_RS02060 at 521.626 kb on - strand, within CA265_RS02060 at 521.652 kb on - strand, within CA265_RS02060 at 521.676 kb on - strand, within CA265_RS02060 at 521.709 kb on + strand, within CA265_RS02060 at 521.709 kb on + strand at 521.710 kb on - strand, within CA265_RS02060 at 521.748 kb on + strand, within CA265_RS02060 at 521.791 kb on - strand, within CA265_RS02060 at 521.798 kb on + strand, within CA265_RS02060 at 521.798 kb on + strand, within CA265_RS02060 at 521.799 kb on - strand, within CA265_RS02060 at 521.799 kb on - strand, within CA265_RS02060 at 521.799 kb on - strand, within CA265_RS02060 at 521.802 kb on + strand, within CA265_RS02060 at 521.806 kb on - strand, within CA265_RS02060 at 521.868 kb on + strand, within CA265_RS02060 at 521.871 kb on + strand, within CA265_RS02060 at 521.899 kb on - strand, within CA265_RS02060 at 521.904 kb on + strand, within CA265_RS02060 at 521.905 kb on - strand, within CA265_RS02060 at 521.907 kb on - strand, within CA265_RS02060 at 521.974 kb on + strand, within CA265_RS02060 at 522.006 kb on + strand, within CA265_RS02060 at 522.147 kb on + strand, within CA265_RS02060 at 522.231 kb on + strand, within CA265_RS02060 at 522.232 kb on - strand, within CA265_RS02060 at 522.316 kb on + strand, within CA265_RS02060 at 522.414 kb on - strand, within CA265_RS02060 at 522.414 kb on - strand, within CA265_RS02060 at 522.414 kb on - strand, within CA265_RS02060 at 522.431 kb on + strand, within CA265_RS02060 at 522.452 kb on - strand, within CA265_RS02060 at 522.513 kb on - strand, within CA265_RS02060 at 522.535 kb on - strand, within CA265_RS02060 at 522.535 kb on - strand, within CA265_RS02060 at 522.544 kb on - strand, within CA265_RS02060 at 522.612 kb on + strand, within CA265_RS02060 at 522.682 kb on + strand at 522.688 kb on + strand at 522.688 kb on + strand at 522.713 kb on + strand at 522.714 kb on - strand at 522.754 kb on - strand at 522.775 kb on - strand at 522.811 kb on + strand at 522.850 kb on + strand at 522.853 kb on - strand at 522.902 kb on - strand at 522.902 kb on - strand at 522.903 kb on + strand at 522.947 kb on - strand at 522.971 kb on + strand, within CA265_RS02065 at 523.043 kb on + strand, within CA265_RS02065 at 523.043 kb on + strand, within CA265_RS02065 at 523.044 kb on - strand, within CA265_RS02065 at 523.055 kb on - strand, within CA265_RS02065 at 523.055 kb on - strand, within CA265_RS02065 at 523.165 kb on - strand, within CA265_RS02065 at 523.175 kb on + strand, within CA265_RS02065 at 523.262 kb on - strand, within CA265_RS02065 at 523.271 kb on + strand, within CA265_RS02065 at 523.271 kb on + strand, within CA265_RS02065 at 523.272 kb on - strand, within CA265_RS02065 at 523.274 kb on - strand, within CA265_RS02065 at 523.274 kb on - strand, within CA265_RS02065 at 523.291 kb on + strand, within CA265_RS02065 at 523.350 kb on - strand, within CA265_RS02065 at 523.498 kb on + strand at 523.499 kb on - strand at 523.518 kb on + strand at 523.568 kb on + strand at 523.599 kb on + strand at 523.600 kb on - strand at 523.638 kb on + strand at 523.639 kb on - strand at 523.660 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.016 mg/ml remove 521,302 - -0.8 521,304 - +1.7 521,395 - +1.1 521,398 - -0.2 521,442 - -0.1 521,517 - CA265_RS02060 0.13 +1.4 521,517 - CA265_RS02060 0.13 -0.1 521,518 + CA265_RS02060 0.13 +1.5 521,518 + CA265_RS02060 0.13 -0.5 521,519 - CA265_RS02060 0.13 +0.5 521,519 - CA265_RS02060 0.13 -1.6 521,519 - CA265_RS02060 0.13 -0.9 521,519 - CA265_RS02060 0.13 -0.3 521,519 - CA265_RS02060 0.13 -0.3 521,535 + CA265_RS02060 0.14 +0.0 521,619 + CA265_RS02060 0.20 -0.4 521,619 + CA265_RS02060 0.20 -1.0 521,620 - CA265_RS02060 0.20 -0.2 521,620 - +1.5 521,620 - CA265_RS02060 0.20 +0.4 521,620 - CA265_RS02060 0.20 -0.0 521,626 - CA265_RS02060 0.20 -0.5 521,652 - CA265_RS02060 0.22 -0.7 521,676 - CA265_RS02060 0.23 +0.1 521,709 + CA265_RS02060 0.26 +0.0 521,709 + +0.5 521,710 - CA265_RS02060 0.26 -0.5 521,748 + CA265_RS02060 0.28 +0.3 521,791 - CA265_RS02060 0.31 -0.5 521,798 + CA265_RS02060 0.32 +0.3 521,798 + CA265_RS02060 0.32 +0.0 521,799 - CA265_RS02060 0.32 -0.2 521,799 - CA265_RS02060 0.32 +0.3 521,799 - CA265_RS02060 0.32 -1.8 521,802 + CA265_RS02060 0.32 +0.8 521,806 - CA265_RS02060 0.32 -0.8 521,868 + CA265_RS02060 0.36 -0.0 521,871 + CA265_RS02060 0.36 -0.8 521,899 - CA265_RS02060 0.38 +0.5 521,904 + CA265_RS02060 0.39 +0.4 521,905 - CA265_RS02060 0.39 -0.2 521,907 - CA265_RS02060 0.39 +0.3 521,974 + CA265_RS02060 0.43 +0.6 522,006 + CA265_RS02060 0.45 +1.4 522,147 + CA265_RS02060 0.55 +0.5 522,231 + CA265_RS02060 0.60 +1.3 522,232 - CA265_RS02060 0.60 -0.5 522,316 + CA265_RS02060 0.66 +0.8 522,414 - CA265_RS02060 0.73 +1.3 522,414 - CA265_RS02060 0.73 +0.2 522,414 - CA265_RS02060 0.73 +0.6 522,431 + CA265_RS02060 0.74 +0.3 522,452 - CA265_RS02060 0.75 -0.7 522,513 - CA265_RS02060 0.79 +0.4 522,535 - CA265_RS02060 0.81 +1.4 522,535 - CA265_RS02060 0.81 -0.5 522,544 - CA265_RS02060 0.81 -1.0 522,612 + CA265_RS02060 0.86 +0.5 522,682 + -0.1 522,688 + -0.6 522,688 + +0.1 522,713 + -0.3 522,714 - -0.2 522,754 - +0.5 522,775 - -0.1 522,811 + +0.7 522,850 + +0.3 522,853 - +0.1 522,902 - -0.1 522,902 - +0.4 522,903 + +0.9 522,947 - -1.3 522,971 + CA265_RS02065 0.16 -0.7 523,043 + CA265_RS02065 0.27 +1.5 523,043 + CA265_RS02065 0.27 +0.3 523,044 - CA265_RS02065 0.27 -0.1 523,055 - CA265_RS02065 0.28 +0.1 523,055 - CA265_RS02065 0.28 +0.9 523,165 - CA265_RS02065 0.45 -0.1 523,175 + CA265_RS02065 0.46 -0.1 523,262 - CA265_RS02065 0.59 +1.3 523,271 + CA265_RS02065 0.60 -0.8 523,271 + CA265_RS02065 0.60 +0.2 523,272 - CA265_RS02065 0.61 -1.2 523,274 - CA265_RS02065 0.61 -0.3 523,274 - CA265_RS02065 0.61 +1.7 523,291 + CA265_RS02065 0.63 +0.0 523,350 - CA265_RS02065 0.72 -0.4 523,498 + +0.5 523,499 - -0.2 523,518 + -0.2 523,568 + -0.2 523,599 + -0.0 523,600 - +0.9 523,638 + +0.3 523,639 - +0.2 523,660 + -0.1
Or see this region's nucleotide sequence