Experiment: Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; 1,3-Propanediol_timecourse2; 10 generations
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS01250 and MMP_RS01255 are separated by 33 nucleotides MMP_RS01255 and MMP_RS01260 are separated by 40 nucleotides MMP_RS01260 and MMP_RS01265 are separated by 81 nucleotides MMP_RS01265 and MMP_RS01270 are separated by 14 nucleotides
MMP_RS01250: MMP_RS01250 - tRNA (guanine(26)-N(2))-dimethyltransferase, at 236,852 to 237,973
_RS01250
MMP_RS01255: MMP_RS01255 - amino acid ABC transporter ATP-binding protein, at 238,007 to 238,738
_RS01255
MMP_RS01260: MMP_RS01260 - HDIG domain-containing protein, at 238,779 to 239,465
_RS01260
MMP_RS01265: MMP_RS01265 - hypothetical protein, at 239,547 to 239,915
_RS01265
MMP_RS01270: MMP_RS01270 - prepilin peptidase, at 239,930 to 240,970
_RS01270
Position (kb) 238
239
240 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1 at 237.872 kb on + strand at 237.877 kb on - strand at 237.983 kb on + strand at 237.989 kb on - strand at 237.993 kb on - strand at 237.994 kb on + strand at 237.994 kb on + strand at 238.009 kb on + strand at 238.066 kb on - strand at 238.067 kb on + strand at 238.205 kb on - strand, within MMP_RS01255 at 238.215 kb on + strand, within MMP_RS01255 at 238.262 kb on - strand, within MMP_RS01255 at 238.450 kb on - strand, within MMP_RS01255 at 238.646 kb on - strand, within MMP_RS01255 at 239.228 kb on + strand, within MMP_RS01260 at 239.394 kb on - strand, within MMP_RS01260 at 239.394 kb on - strand, within MMP_RS01260 at 239.394 kb on - strand, within MMP_RS01260 at 239.397 kb on + strand at 239.454 kb on - strand at 239.454 kb on - strand at 239.482 kb on - strand at 239.482 kb on - strand at 239.483 kb on + strand at 239.485 kb on + strand at 239.486 kb on - strand at 239.499 kb on + strand at 239.512 kb on + strand at 239.544 kb on - strand at 239.544 kb on - strand at 239.546 kb on - strand at 239.546 kb on - strand at 239.546 kb on - strand at 239.547 kb on + strand at 239.804 kb on - strand, within MMP_RS01265 at 239.808 kb on + strand, within MMP_RS01265 at 239.868 kb on + strand, within MMP_RS01265 at 239.869 kb on - strand, within MMP_RS01265 at 239.999 kb on - strand at 240.000 kb on + strand at 240.001 kb on - strand at 240.001 kb on - strand at 240.052 kb on + strand, within MMP_RS01270 at 240.132 kb on - strand, within MMP_RS01270 at 240.166 kb on - strand, within MMP_RS01270 at 240.167 kb on + strand, within MMP_RS01270 at 240.175 kb on - strand, within MMP_RS01270 at 240.208 kb on - strand, within MMP_RS01270 at 240.208 kb on - strand, within MMP_RS01270 at 240.208 kb on - strand, within MMP_RS01270 at 240.209 kb on + strand, within MMP_RS01270 at 240.212 kb on - strand, within MMP_RS01270 at 240.213 kb on + strand, within MMP_RS01270 at 240.213 kb on + strand, within MMP_RS01270 at 240.249 kb on - strand, within MMP_RS01270 at 240.250 kb on + strand, within MMP_RS01270 at 240.311 kb on + strand, within MMP_RS01270 at 240.354 kb on - strand, within MMP_RS01270 at 240.400 kb on + strand, within MMP_RS01270 at 240.415 kb on + strand, within MMP_RS01270
Per-strain Table
Position Strand Gene LocusTag Fraction Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; 1,3-Propanediol_timecourse2; 10 generations remove 237,872 + -1.8 237,877 - -2.4 237,983 + +0.2 237,989 - +0.8 237,993 - -0.5 237,994 + +0.1 237,994 + -0.4 238,009 + +0.5 238,066 - +0.4 238,067 + -0.0 238,205 - MMP_RS01255 0.27 +0.1 238,215 + MMP_RS01255 0.28 -0.0 238,262 - MMP_RS01255 0.35 -0.3 238,450 - MMP_RS01255 0.61 -0.2 238,646 - MMP_RS01255 0.87 +0.1 239,228 + MMP_RS01260 0.65 -0.5 239,394 - MMP_RS01260 0.90 -0.1 239,394 - MMP_RS01260 0.90 +0.7 239,394 - MMP_RS01260 0.90 +0.4 239,397 + -0.5 239,454 - +0.1 239,454 - +0.5 239,482 - +0.2 239,482 - +0.7 239,483 + -0.2 239,485 + -0.1 239,486 - +0.5 239,499 + +0.3 239,512 + +0.1 239,544 - -3.3 239,544 - -2.8 239,546 - -3.0 239,546 - -4.1 239,546 - -3.5 239,547 + -3.1 239,804 - MMP_RS01265 0.70 -6.0 239,808 + MMP_RS01265 0.71 -4.7 239,868 + MMP_RS01265 0.87 -5.9 239,869 - MMP_RS01265 0.87 -2.6 239,999 - -1.3 240,000 + +0.2 240,001 - -0.4 240,001 - -0.1 240,052 + MMP_RS01270 0.12 -0.7 240,132 - MMP_RS01270 0.19 +0.1 240,166 - MMP_RS01270 0.23 -0.1 240,167 + MMP_RS01270 0.23 +0.2 240,175 - MMP_RS01270 0.24 -0.0 240,208 - MMP_RS01270 0.27 +0.4 240,208 - MMP_RS01270 0.27 -0.3 240,208 - MMP_RS01270 0.27 -0.6 240,209 + MMP_RS01270 0.27 -0.6 240,212 - MMP_RS01270 0.27 -0.3 240,213 + MMP_RS01270 0.27 -0.3 240,213 + MMP_RS01270 0.27 -0.7 240,249 - MMP_RS01270 0.31 +0.1 240,250 + MMP_RS01270 0.31 -0.1 240,311 + MMP_RS01270 0.37 -0.0 240,354 - MMP_RS01270 0.41 -0.4 240,400 + MMP_RS01270 0.45 +0.1 240,415 + MMP_RS01270 0.47 -0.2
Or see this region's nucleotide sequence