Experiment: Nickel (II) chloride 40 uM, aerobic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_02085 and IAI47_02090 are separated by 68 nucleotides IAI47_02090 and IAI47_02095 are separated by 171 nucleotides IAI47_02095 and IAI47_02100 are separated by 99 nucleotides IAI47_02100 and IAI47_02105 overlap by 14 nucleotides
IAI47_02085: IAI47_02085 - elongation factor G, at 448,713 to 450,827
_02085
IAI47_02090: IAI47_02090 - elongation factor Tu, at 450,896 to 452,080
_02090
IAI47_02095: IAI47_02095 - bacterioferritin-associated ferredoxin, at 452,252 to 452,446
_02095
IAI47_02100: IAI47_02100 - bacterioferritin, at 452,546 to 453,019
_02100
IAI47_02105: IAI47_02105 - prepilin peptidase, at 453,006 to 453,779
_02105
Position (kb) 450
451
452
453 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 450.825 kb on + strand at 450.826 kb on - strand at 450.881 kb on + strand at 450.881 kb on + strand at 450.882 kb on - strand at 450.882 kb on - strand at 450.889 kb on + strand at 450.967 kb on + strand at 450.999 kb on + strand at 450.999 kb on + strand at 451.006 kb on + strand at 451.075 kb on + strand, within IAI47_02090 at 451.103 kb on + strand, within IAI47_02090 at 451.157 kb on + strand, within IAI47_02090 at 451.158 kb on - strand, within IAI47_02090 at 451.344 kb on + strand, within IAI47_02090 at 451.376 kb on + strand, within IAI47_02090 at 451.376 kb on + strand, within IAI47_02090 at 451.376 kb on + strand, within IAI47_02090 at 451.376 kb on + strand, within IAI47_02090 at 451.376 kb on + strand, within IAI47_02090 at 451.377 kb on - strand, within IAI47_02090 at 451.377 kb on - strand, within IAI47_02090 at 451.486 kb on + strand, within IAI47_02090 at 451.490 kb on + strand, within IAI47_02090 at 451.490 kb on + strand, within IAI47_02090 at 451.490 kb on + strand, within IAI47_02090 at 451.491 kb on - strand, within IAI47_02090 at 451.491 kb on - strand, within IAI47_02090 at 452.065 kb on + strand at 452.078 kb on + strand at 452.078 kb on + strand at 452.078 kb on + strand at 452.078 kb on + strand at 452.087 kb on + strand at 452.096 kb on + strand at 452.096 kb on + strand at 452.096 kb on + strand at 452.096 kb on + strand at 452.097 kb on - strand at 452.097 kb on - strand at 452.126 kb on + strand at 452.126 kb on + strand at 452.126 kb on + strand at 452.126 kb on + strand at 452.127 kb on - strand at 452.164 kb on + strand at 452.164 kb on + strand at 452.171 kb on + strand at 452.205 kb on - strand at 452.224 kb on - strand at 452.226 kb on - strand at 452.226 kb on - strand at 452.255 kb on + strand at 452.255 kb on + strand at 452.255 kb on + strand at 452.255 kb on + strand at 452.255 kb on + strand at 452.255 kb on + strand at 452.255 kb on + strand at 452.255 kb on + strand at 452.255 kb on + strand at 452.255 kb on + strand at 452.255 kb on + strand at 452.256 kb on - strand at 452.256 kb on - strand at 452.256 kb on - strand at 452.256 kb on - strand at 452.256 kb on - strand at 452.256 kb on - strand at 452.256 kb on - strand at 452.256 kb on - strand at 452.269 kb on + strand at 452.269 kb on + strand at 452.269 kb on + strand at 452.269 kb on + strand at 452.269 kb on + strand at 452.269 kb on + strand at 452.270 kb on - strand at 452.270 kb on - strand at 452.270 kb on - strand at 452.270 kb on - strand at 452.270 kb on - strand at 452.270 kb on - strand at 452.270 kb on - strand at 452.270 kb on - strand at 452.270 kb on - strand at 452.284 kb on + strand, within IAI47_02095 at 452.284 kb on + strand, within IAI47_02095 at 452.284 kb on + strand, within IAI47_02095 at 452.284 kb on + strand, within IAI47_02095 at 452.284 kb on + strand, within IAI47_02095 at 452.285 kb on - strand, within IAI47_02095 at 452.285 kb on - strand, within IAI47_02095 at 452.312 kb on + strand, within IAI47_02095 at 452.312 kb on + strand, within IAI47_02095 at 452.313 kb on - strand, within IAI47_02095 at 452.327 kb on - strand, within IAI47_02095 at 452.355 kb on + strand, within IAI47_02095 at 452.356 kb on - strand, within IAI47_02095 at 452.356 kb on - strand, within IAI47_02095 at 452.356 kb on - strand, within IAI47_02095 at 452.429 kb on + strand at 452.430 kb on - strand at 452.430 kb on - strand at 452.431 kb on + strand at 452.432 kb on - strand at 452.432 kb on - strand at 452.439 kb on + strand at 452.439 kb on + strand at 452.440 kb on - strand at 452.440 kb on - strand at 452.440 kb on - strand at 452.440 kb on - strand at 452.440 kb on - strand at 452.440 kb on - strand at 452.466 kb on - strand at 452.474 kb on + strand at 452.474 kb on + strand at 452.474 kb on + strand at 452.474 kb on + strand at 452.475 kb on - strand at 452.475 kb on - strand at 452.475 kb on - strand at 452.475 kb on - strand at 452.475 kb on - strand at 452.475 kb on - strand at 452.477 kb on - strand at 452.512 kb on + strand at 452.512 kb on + strand at 452.512 kb on + strand at 452.513 kb on - strand at 452.513 kb on - strand at 452.519 kb on + strand at 452.520 kb on - strand at 452.520 kb on - strand at 452.520 kb on - strand at 452.520 kb on - strand at 452.523 kb on - strand at 452.523 kb on - strand at 452.541 kb on + strand at 452.542 kb on - strand at 452.558 kb on - strand at 452.558 kb on - strand at 452.567 kb on - strand at 452.569 kb on + strand at 452.569 kb on + strand at 452.569 kb on + strand at 452.570 kb on - strand at 452.581 kb on + strand at 452.619 kb on - strand, within IAI47_02100 at 452.678 kb on + strand, within IAI47_02100 at 452.679 kb on - strand, within IAI47_02100 at 452.717 kb on + strand, within IAI47_02100 at 452.717 kb on + strand, within IAI47_02100 at 452.717 kb on + strand, within IAI47_02100 at 452.717 kb on + strand, within IAI47_02100 at 452.717 kb on + strand, within IAI47_02100 at 452.717 kb on + strand, within IAI47_02100 at 452.717 kb on + strand, within IAI47_02100 at 452.718 kb on - strand, within IAI47_02100 at 452.718 kb on - strand, within IAI47_02100 at 452.718 kb on - strand, within IAI47_02100 at 452.718 kb on - strand, within IAI47_02100 at 452.718 kb on - strand, within IAI47_02100 at 452.718 kb on - strand, within IAI47_02100 at 452.718 kb on - strand, within IAI47_02100 at 452.718 kb on - strand, within IAI47_02100 at 452.718 kb on - strand, within IAI47_02100 at 452.718 kb on - strand, within IAI47_02100 at 452.718 kb on - strand, within IAI47_02100 at 452.718 kb on - strand, within IAI47_02100 at 452.718 kb on - strand, within IAI47_02100 at 452.719 kb on + strand, within IAI47_02100 at 452.720 kb on - strand, within IAI47_02100 at 452.720 kb on - strand, within IAI47_02100 at 452.720 kb on - strand, within IAI47_02100 at 452.720 kb on - strand, within IAI47_02100 at 452.780 kb on - strand, within IAI47_02100 at 452.839 kb on + strand, within IAI47_02100 at 452.840 kb on - strand, within IAI47_02100 at 452.857 kb on + strand, within IAI47_02100 at 452.857 kb on + strand, within IAI47_02100 at 452.857 kb on + strand, within IAI47_02100 at 452.857 kb on + strand, within IAI47_02100 at 452.857 kb on + strand, within IAI47_02100 at 452.857 kb on + strand, within IAI47_02100 at 452.857 kb on + strand, within IAI47_02100 at 452.858 kb on - strand, within IAI47_02100 at 452.858 kb on - strand, within IAI47_02100 at 452.858 kb on - strand, within IAI47_02100 at 452.864 kb on + strand, within IAI47_02100 at 452.864 kb on + strand, within IAI47_02100 at 452.864 kb on + strand, within IAI47_02100 at 452.864 kb on + strand, within IAI47_02100 at 452.864 kb on + strand, within IAI47_02100 at 452.864 kb on + strand, within IAI47_02100 at 452.864 kb on + strand, within IAI47_02100 at 452.864 kb on + strand, within IAI47_02100 at 452.864 kb on + strand, within IAI47_02100 at 452.865 kb on - strand, within IAI47_02100 at 452.865 kb on - strand, within IAI47_02100 at 452.865 kb on - strand, within IAI47_02100 at 452.865 kb on - strand, within IAI47_02100 at 452.865 kb on - strand, within IAI47_02100 at 452.865 kb on - strand, within IAI47_02100 at 452.865 kb on - strand, within IAI47_02100 at 452.865 kb on - strand, within IAI47_02100 at 452.865 kb on - strand, within IAI47_02100 at 452.872 kb on + strand, within IAI47_02100 at 452.872 kb on + strand, within IAI47_02100 at 452.873 kb on - strand, within IAI47_02100 at 452.873 kb on - strand, within IAI47_02100 at 452.885 kb on + strand, within IAI47_02100 at 452.885 kb on + strand, within IAI47_02100 at 452.885 kb on + strand, within IAI47_02100 at 452.885 kb on + strand, within IAI47_02100 at 452.885 kb on + strand, within IAI47_02100 at 452.885 kb on + strand, within IAI47_02100 at 452.885 kb on + strand, within IAI47_02100 at 452.885 kb on + strand, within IAI47_02100 at 452.885 kb on + strand, within IAI47_02100 at 452.885 kb on + strand, within IAI47_02100 at 452.885 kb on + strand, within IAI47_02100 at 452.885 kb on + strand, within IAI47_02100 at 452.885 kb on + strand, within IAI47_02100 at 452.886 kb on - strand, within IAI47_02100 at 452.886 kb on - strand, within IAI47_02100 at 452.886 kb on - strand, within IAI47_02100 at 452.886 kb on - strand, within IAI47_02100 at 452.886 kb on - strand, within IAI47_02100 at 452.906 kb on + strand, within IAI47_02100 at 452.990 kb on + strand at 452.990 kb on + strand at 452.990 kb on + strand at 452.990 kb on + strand at 452.990 kb on + strand at 452.990 kb on + strand at 452.990 kb on + strand at 452.991 kb on - strand at 452.991 kb on - strand at 452.991 kb on - strand at 453.033 kb on + strand at 453.033 kb on + strand at 453.033 kb on + strand at 453.033 kb on + strand at 453.033 kb on + strand at 453.033 kb on + strand at 453.033 kb on + strand at 453.034 kb on - strand at 453.034 kb on - strand at 453.034 kb on - strand at 453.034 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) chloride 40 uM, aerobic remove 450,825 + -1.6 450,826 - +0.6 450,881 + +0.1 450,881 + +0.4 450,882 - -0.4 450,882 - +0.3 450,889 + +1.5 450,967 + -0.6 450,999 + +0.3 450,999 + -0.4 451,006 + -0.2 451,075 + IAI47_02090 0.15 +0.6 451,103 + IAI47_02090 0.17 -0.2 451,157 + IAI47_02090 0.22 -0.7 451,158 - IAI47_02090 0.22 -0.7 451,344 + IAI47_02090 0.38 -0.4 451,376 + IAI47_02090 0.41 -1.0 451,376 + IAI47_02090 0.41 -0.7 451,376 + IAI47_02090 0.41 -0.6 451,376 + IAI47_02090 0.41 +1.1 451,376 + IAI47_02090 0.41 -0.9 451,377 - IAI47_02090 0.41 +1.0 451,377 - IAI47_02090 0.41 +0.4 451,486 + IAI47_02090 0.50 -0.0 451,490 + IAI47_02090 0.50 -0.3 451,490 + IAI47_02090 0.50 -1.0 451,490 + IAI47_02090 0.50 -1.2 451,491 - IAI47_02090 0.50 +0.1 451,491 - IAI47_02090 0.50 -0.3 452,065 + -1.6 452,078 + +0.1 452,078 + -0.3 452,078 + +0.4 452,078 + +0.4 452,087 + -0.6 452,096 + -0.7 452,096 + -0.5 452,096 + -0.4 452,096 + +0.8 452,097 - -0.7 452,097 - -1.2 452,126 + -1.1 452,126 + -1.7 452,126 + +1.1 452,126 + -1.1 452,127 - -0.3 452,164 + -0.7 452,164 + +0.5 452,171 + -0.6 452,205 - +2.0 452,224 - +0.3 452,226 - -0.1 452,226 - -1.2 452,255 + +1.6 452,255 + +0.1 452,255 + +0.3 452,255 + +0.6 452,255 + -0.2 452,255 + +2.8 452,255 + +1.2 452,255 + +0.2 452,255 + +0.1 452,255 + +0.1 452,255 + +0.3 452,256 - -0.2 452,256 - -1.4 452,256 - +0.1 452,256 - -0.4 452,256 - +0.3 452,256 - +0.5 452,256 - +0.7 452,256 - -1.4 452,269 + -1.0 452,269 + +1.1 452,269 + -1.2 452,269 + -1.1 452,269 + +1.2 452,269 + -0.0 452,270 - -0.6 452,270 - +1.3 452,270 - +0.7 452,270 - -1.0 452,270 - -0.5 452,270 - +0.2 452,270 - +0.8 452,270 - +0.4 452,270 - +0.9 452,284 + IAI47_02095 0.16 +0.9 452,284 + IAI47_02095 0.16 -0.1 452,284 + IAI47_02095 0.16 -0.1 452,284 + IAI47_02095 0.16 +0.4 452,284 + IAI47_02095 0.16 +0.0 452,285 - IAI47_02095 0.17 -1.0 452,285 - IAI47_02095 0.17 +0.7 452,312 + IAI47_02095 0.31 -1.3 452,312 + IAI47_02095 0.31 +0.7 452,313 - IAI47_02095 0.31 +0.4 452,327 - IAI47_02095 0.38 +0.6 452,355 + IAI47_02095 0.53 -0.1 452,356 - IAI47_02095 0.53 +0.6 452,356 - IAI47_02095 0.53 +0.8 452,356 - IAI47_02095 0.53 -0.4 452,429 + -0.2 452,430 - +0.7 452,430 - +0.3 452,431 + +0.1 452,432 - -0.4 452,432 - +2.6 452,439 + -2.3 452,439 + +1.0 452,440 - +1.2 452,440 - +0.8 452,440 - -0.6 452,440 - +1.0 452,440 - +0.5 452,440 - -0.6 452,466 - -0.8 452,474 + +0.8 452,474 + -0.2 452,474 + +1.6 452,474 + -0.2 452,475 - +1.2 452,475 - +0.3 452,475 - -1.4 452,475 - -0.6 452,475 - -0.5 452,475 - +0.4 452,477 - +0.9 452,512 + +2.0 452,512 + -0.3 452,512 + -0.6 452,513 - -0.7 452,513 - +1.4 452,519 + -0.8 452,520 - +0.4 452,520 - +0.1 452,520 - -0.1 452,520 - -0.2 452,523 - -0.0 452,523 - +1.0 452,541 + +0.6 452,542 - +1.4 452,558 - +0.2 452,558 - +0.3 452,567 - -0.4 452,569 + -1.0 452,569 + +1.5 452,569 + +2.0 452,570 - -0.1 452,581 + -2.9 452,619 - IAI47_02100 0.15 +0.5 452,678 + IAI47_02100 0.28 +0.6 452,679 - IAI47_02100 0.28 -1.1 452,717 + IAI47_02100 0.36 -0.1 452,717 + IAI47_02100 0.36 +0.1 452,717 + IAI47_02100 0.36 +1.0 452,717 + IAI47_02100 0.36 -1.0 452,717 + IAI47_02100 0.36 -0.3 452,717 + IAI47_02100 0.36 +0.9 452,717 + IAI47_02100 0.36 -0.3 452,718 - IAI47_02100 0.36 -0.2 452,718 - IAI47_02100 0.36 +1.6 452,718 - IAI47_02100 0.36 +1.2 452,718 - IAI47_02100 0.36 +0.5 452,718 - IAI47_02100 0.36 +0.8 452,718 - IAI47_02100 0.36 +0.4 452,718 - IAI47_02100 0.36 -0.1 452,718 - IAI47_02100 0.36 +0.4 452,718 - IAI47_02100 0.36 -1.3 452,718 - IAI47_02100 0.36 +0.9 452,718 - IAI47_02100 0.36 -0.2 452,718 - IAI47_02100 0.36 +1.0 452,718 - IAI47_02100 0.36 +0.0 452,719 + IAI47_02100 0.36 -0.9 452,720 - IAI47_02100 0.37 +0.5 452,720 - IAI47_02100 0.37 -0.1 452,720 - IAI47_02100 0.37 +1.3 452,720 - IAI47_02100 0.37 -0.7 452,780 - IAI47_02100 0.49 -1.9 452,839 + IAI47_02100 0.62 +1.0 452,840 - IAI47_02100 0.62 -0.7 452,857 + IAI47_02100 0.66 -0.2 452,857 + IAI47_02100 0.66 +1.1 452,857 + IAI47_02100 0.66 -0.7 452,857 + IAI47_02100 0.66 -0.3 452,857 + IAI47_02100 0.66 +1.1 452,857 + IAI47_02100 0.66 -0.2 452,857 + IAI47_02100 0.66 +1.0 452,858 - IAI47_02100 0.66 +0.3 452,858 - IAI47_02100 0.66 +0.4 452,858 - IAI47_02100 0.66 -0.6 452,864 + IAI47_02100 0.67 +0.4 452,864 + IAI47_02100 0.67 +1.1 452,864 + IAI47_02100 0.67 +0.4 452,864 + IAI47_02100 0.67 +0.8 452,864 + IAI47_02100 0.67 +0.2 452,864 + IAI47_02100 0.67 +0.8 452,864 + IAI47_02100 0.67 -0.0 452,864 + IAI47_02100 0.67 +1.0 452,864 + IAI47_02100 0.67 +0.6 452,865 - IAI47_02100 0.67 -0.0 452,865 - IAI47_02100 0.67 +0.6 452,865 - IAI47_02100 0.67 -0.2 452,865 - IAI47_02100 0.67 -0.4 452,865 - IAI47_02100 0.67 +0.3 452,865 - IAI47_02100 0.67 -0.8 452,865 - IAI47_02100 0.67 +0.2 452,865 - IAI47_02100 0.67 +0.7 452,865 - IAI47_02100 0.67 -1.0 452,872 + IAI47_02100 0.69 -0.3 452,872 + IAI47_02100 0.69 +1.0 452,873 - IAI47_02100 0.69 +0.3 452,873 - IAI47_02100 0.69 +0.6 452,885 + IAI47_02100 0.72 +0.7 452,885 + IAI47_02100 0.72 +0.7 452,885 + IAI47_02100 0.72 +0.4 452,885 + IAI47_02100 0.72 +0.0 452,885 + IAI47_02100 0.72 -0.3 452,885 + IAI47_02100 0.72 -0.1 452,885 + IAI47_02100 0.72 -0.6 452,885 + IAI47_02100 0.72 +1.6 452,885 + IAI47_02100 0.72 +1.0 452,885 + IAI47_02100 0.72 +0.3 452,885 + IAI47_02100 0.72 -0.3 452,885 + IAI47_02100 0.72 -1.4 452,885 + IAI47_02100 0.72 +0.4 452,886 - IAI47_02100 0.72 +0.4 452,886 - IAI47_02100 0.72 +1.8 452,886 - IAI47_02100 0.72 -0.1 452,886 - IAI47_02100 0.72 -1.0 452,886 - IAI47_02100 0.72 -0.8 452,906 + IAI47_02100 0.76 +1.3 452,990 + +0.7 452,990 + +0.4 452,990 + -0.1 452,990 + +0.1 452,990 + +1.0 452,990 + +0.3 452,990 + +0.1 452,991 - +0.4 452,991 - -0.0 452,991 - +0.2 453,033 + -1.2 453,033 + -0.9 453,033 + -0.6 453,033 + +1.5 453,033 + +0.3 453,033 + +0.0 453,033 + +1.5 453,034 - +0.7 453,034 - -0.6 453,034 - -1.0 453,034 - -0.4
Or see this region's nucleotide sequence