Experiment: Ammonium chloride (N); RCH2 media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS25020 and EX31_RS23785 are separated by 14 nucleotides EX31_RS23785 and EX31_RS23790 are separated by 319 nucleotides
EX31_RS25020: EX31_RS25020 - hypothetical protein, at 521,070 to 522,320
_RS25020
EX31_RS23785: EX31_RS23785 - DUF262 domain-containing protein, at 522,335 to 524,656
_RS23785
EX31_RS23790: EX31_RS23790 - transcriptional regulator, at 524,976 to 525,629
_RS23790
Position (kb) 522
523
524
525 Strain fitness (log2 ratio) -2
-1
0
1
2 at 521.500 kb on + strand, within EX31_RS25020 at 521.502 kb on + strand, within EX31_RS25020 at 521.548 kb on + strand, within EX31_RS25020 at 521.644 kb on - strand, within EX31_RS25020 at 521.802 kb on + strand, within EX31_RS25020 at 521.993 kb on + strand, within EX31_RS25020 at 522.044 kb on + strand, within EX31_RS25020 at 522.178 kb on - strand, within EX31_RS25020 at 522.539 kb on - strand at 522.765 kb on + strand, within EX31_RS23785 at 523.041 kb on - strand, within EX31_RS23785 at 523.100 kb on - strand, within EX31_RS23785 at 523.221 kb on + strand, within EX31_RS23785 at 523.525 kb on - strand, within EX31_RS23785 at 523.544 kb on - strand, within EX31_RS23785 at 523.831 kb on + strand, within EX31_RS23785 at 523.966 kb on - strand, within EX31_RS23785 at 524.089 kb on - strand, within EX31_RS23785 at 524.632 kb on + strand at 524.737 kb on + strand at 524.759 kb on - strand at 524.769 kb on - strand at 524.769 kb on - strand at 524.774 kb on - strand at 524.774 kb on - strand at 524.826 kb on + strand at 524.827 kb on - strand at 524.868 kb on - strand at 524.917 kb on - strand at 524.923 kb on - strand at 524.923 kb on - strand at 524.923 kb on - strand at 524.935 kb on - strand at 524.963 kb on - strand at 524.974 kb on + strand at 524.995 kb on + strand at 525.024 kb on - strand at 525.067 kb on - strand, within EX31_RS23790 at 525.067 kb on - strand, within EX31_RS23790 at 525.067 kb on - strand, within EX31_RS23790 at 525.067 kb on - strand, within EX31_RS23790 at 525.067 kb on - strand, within EX31_RS23790 at 525.145 kb on + strand, within EX31_RS23790 at 525.146 kb on - strand, within EX31_RS23790 at 525.180 kb on - strand, within EX31_RS23790 at 525.181 kb on + strand, within EX31_RS23790 at 525.182 kb on - strand, within EX31_RS23790 at 525.182 kb on - strand, within EX31_RS23790 at 525.182 kb on - strand, within EX31_RS23790 at 525.182 kb on - strand, within EX31_RS23790 at 525.595 kb on - strand at 525.629 kb on + strand at 525.630 kb on - strand at 525.630 kb on - strand at 525.652 kb on + strand at 525.652 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N); RCH2 media; shaking remove 521,500 + EX31_RS25020 0.34 -0.3 521,502 + EX31_RS25020 0.35 +0.1 521,548 + EX31_RS25020 0.38 +1.5 521,644 - EX31_RS25020 0.46 +0.2 521,802 + EX31_RS25020 0.59 -0.4 521,993 + EX31_RS25020 0.74 +0.2 522,044 + EX31_RS25020 0.78 -0.1 522,178 - EX31_RS25020 0.89 +1.4 522,539 - +1.0 522,765 + EX31_RS23785 0.19 -0.2 523,041 - EX31_RS23785 0.30 +0.5 523,100 - EX31_RS23785 0.33 +1.2 523,221 + EX31_RS23785 0.38 -1.0 523,525 - EX31_RS23785 0.51 -1.4 523,544 - EX31_RS23785 0.52 +1.8 523,831 + EX31_RS23785 0.64 +0.1 523,966 - EX31_RS23785 0.70 -0.2 524,089 - EX31_RS23785 0.76 -0.2 524,632 + +0.3 524,737 + -0.6 524,759 - -0.2 524,769 - +0.5 524,769 - +0.2 524,774 - -2.5 524,774 - +1.3 524,826 + +0.2 524,827 - -1.5 524,868 - -0.1 524,917 - +0.8 524,923 - -0.2 524,923 - +0.5 524,923 - -1.0 524,935 - +0.6 524,963 - +1.2 524,974 + +1.3 524,995 + +1.5 525,024 - +0.3 525,067 - EX31_RS23790 0.14 +1.1 525,067 - EX31_RS23790 0.14 -0.9 525,067 - EX31_RS23790 0.14 -0.5 525,067 - EX31_RS23790 0.14 +0.6 525,067 - EX31_RS23790 0.14 -1.5 525,145 + EX31_RS23790 0.26 +0.7 525,146 - EX31_RS23790 0.26 -1.8 525,180 - EX31_RS23790 0.31 +0.1 525,181 + EX31_RS23790 0.31 -0.3 525,182 - EX31_RS23790 0.31 -1.4 525,182 - EX31_RS23790 0.31 +0.8 525,182 - EX31_RS23790 0.31 -0.4 525,182 - EX31_RS23790 0.31 +1.1 525,595 - +0.8 525,629 + +0.1 525,630 - -0.1 525,630 - -2.0 525,652 + -1.0 525,652 + +0.3
Or see this region's nucleotide sequence